Draft Genome Sequence of Fructophilic Lactobacillus florum.

Herein we report the first genome sequence for Lactobacillus florum. L. florum 2F was isolated from Valencia orange leaves and is fructophilic, like other strains of this species. The draft genome of L. florum 2F contains 1,261,842 bp with a G+C content of 41.5% in 46 contigs (≥500 bp)

A new family of diverse skin peptides from the microhylid frog genus phrynomantis

A wide range of frogs produce skin poisons composed of bioactive peptides for defence against pathogens, parasites and predators. While several frog families have been thoroughly screened for skin-secreted peptides, others, like the Microhylidae, have remained mostly unexplored. Previous studies of microhylids found no evidence of peptide secretion, suggesting that this defence adaptation was evolutionarily lost. We conducted transcriptome analyses of the skins of Phrynomantis bifasciatus and Phrynomantis microps, two African microhylid species long suspected to be poisonous. Our analyses reveal 17 evolutionary related transcripts that diversified from to those of cytolytic peptides found in other frog families. The 19 peptides predicted to be processed from these transcripts, named phrynomantins, show a striking structural diversity that is distinct from any previously identified frog skin peptide. Functional analyses of five phrynomantins confirm the loss of a cytolytic function and the absence of insecticidal or proinflammatory activity, suggesting that they represent an evolutionary transition to a new, yet unknown function. Our study shows that peptides have been retained in the defence poison of at least one microhylid lineage and encourages research on similarly understudied taxa to further elucidate the diversity and evolution of skin defence molecules

A combined microfinance and training intervention can reduce HIV risk behaviour in young female participants.

OBJECTIVE: To assess effects of a combined microfinance and training intervention on HIV risk behavior among young female participants in rural South Africa. DESIGN: : Secondary analysis of quantitative and qualitative data from a cluster randomized trial, the Intervention with Microfinance for AIDS and Gender Equity study. METHODS: Eight villages were pair-matched and randomly allocated to receive the intervention. At baseline and after 2 years, HIV risk behavior was assessed among female participants aged 14-35 years. Their responses were compared with women of the same age and poverty group from control villages. Intervention effects were calculated using adjusted risk ratios employing village level summaries. Qualitative data collected during the study explored participants' responses to the intervention including HIV risk behavior. RESULTS: After 2 years of follow-up, when compared with controls, young participants had higher levels of HIV-related communication (adjusted risk ratio 1.46, 95% confidence interval 1.01-2.12), were more likely to have accessed voluntary counseling and testing (adjusted risk ratio 1.64, 95% confidence interval 1.06-2.56), and less likely to have had unprotected sex at last intercourse with a nonspousal partner (adjusted risk ratio 0.76, 95% confidence interval 0.60-0.96). Qualitative data suggest a greater acceptance of intrahousehold communication about HIV and sexuality. Although women noted challenges associated with acceptance of condoms by men, increased confidence and skills associated with participation in the intervention supported their introduction in sexual relationships. CONCLUSIONS: In addition to impacts on economic well being, women's empowerment and intimate partner violence, interventions addressing the economic and social vulnerability of women may contribute to reductions in HIV risk behavior

Demonstration of early functional compromise of bone marrow derived hematopoietic progenitor cells during bovine neonatal pancytopenia through in vitro culture of bone marrow biopsies

<p>Abstract</p> <p>Background</p> <p>Bovine neonatal pancytopenia (BNP) is a syndrome characterised by thrombocytopenia associated with marked bone marrow destruction in calves, widely reported since 2007 in several European countries and since 2011 in New Zealand. The disease is epidemiologically associated with the use of an inactivated bovine virus diarrhoea (BVD) vaccine and is currently considered to be caused by absorption of colostral antibody produced by some vaccinated cows (“BNP dams”). Alloantibodies capable of binding to the leukocyte surface have been detected in BNP dams and antibodies recognising bovine MHC class I and β-2-microglobulin have been detected in vaccinated cattle. In this study, calves were challenged with pooled colostrum collected from BNP dams or from non-BNP dams and their bone marrow hematopoietic progenitor cells (HPC) cultured <it>in vitro</it> from sternal biopsies taken at 24 hours and 6 days post-challenge.</p> <p>Results</p> <p>Clonogenic assay demonstrated that CFU-GEMM (colony forming unit-granulocyte/erythroid/macrophage/megakaryocyte; pluripotential progenitor cell) colony development was compromised from HPCs harvested as early as 24 hour post-challenge. By 6 days post challenge, HPCs harvested from challenged calves failed to develop CFU-E (erythroid) colonies and the development of both CFU-GEMM and CFU-GM (granulocyte/macrophage) was markedly reduced.</p> <p>Conclusion</p> <p>This study suggests that the bone marrow pathology and clinical signs associated with BNP are related to an insult which compromises the pluripotential progenitor cell within the first 24 hours of life but that this does not initially include all cell types.</p

Using DNA Metabarcoding to Characterize the Prey Spectrum of Two Co-Occurring Themisto Amphipods in the Rapidly Changing Atlantic-Arctic Gateway Fram Strait

The two congeneric hyperiids Themisto libellula and T. abyssorum provide an important trophic link between lower and higher trophic levels in the rapidly changing Arctic marine ecosystem. These amphipods are characterized by distinct hydrographic affinities and are hence anticipated to be impacted differently by environmental changes, with major consequences for the Arctic food web. In this study, we applied DNA metabarcoding to the stomach contents of these Themisto species, to comprehensively reveal their prey spectra at an unprecedented-high-taxonomic-resolution and assess the regional variation in their diet across the Fram Strait. Both species feed on a wide variety of prey but their diet strongly differed in the investigated summer season, showing overlap for only a few prey taxa, such as calanoid copepods. The spatially structured prey field of T. libellula clearly differentiated it from T. abyssorum, of which the diet was mainly dominated by chaetognaths. Our approach also allowed the detection of previously overlooked prey in the diet of T. libellula, such as fish species and gelatinous zooplankton. We discuss the reasons for the differences in prey spectra and which consequences these may have in the light of ongoing environmental changes

MODEL DEVELOPMENT AND SYSTEM OPTIMIZATION TO MINIMIZE GREENHOUSE GAS EMISSIONS FROM WASTEWATER TREATMENT PLANTS

As greenhouse gas emissions (GHG) reduction has drawn considerable attention, various methods have been established to estimate greenhouse gas emissions from wastewater treatment plants (WWTPs). In order to establish a design and operational strategy for GHG mitigation, accurate estimates are essential. However, the existing approaches (e.g. the IPCC protocol and national greenhouse gas inventories) do not cover emissions from all sources in WWTPs and are not sufficient to predict facility-level emissions. The ultimate goal of this research was to improve the quantification of GHG emissions from WWTPs. This was accomplished by creating a new mathematical model based on an existing activated sludge model. The first part of the research proposed a stepwise methodology using elemental balances in order to derive stoichiometry for state variables used in a mass balance based whole-plant wastewater treatment plant model. The two main advantages of the elemental balance method are the inclusion of carbon dioxide (CO2) into the existing model with no mass loss and ease of tracking elemental pathways. The second part of the research developed an integrated model that includes (1) a direct emission model for onsite emissions from treatment processes and (2) an indirect emission model for offsite emissions caused by plant operation. A sensitivity analysis of the proposed model was conducted to identify key input parameters. An uncertainty analysis was also carried out using a Monte Carlo simulation, which provided an estimate of the potential variability in GHG estimations. Finally, in the third part, the research identified an optimal operational strategy that resulted in minimizing operating costs and GHG emission, while simultaneously treating the wastewater at better levels. To do this, an integrated performance index (IPI) was proposed to combine the three criteria. The IPI was then incorporated into an optimization algorithm. The results obtained in this research demonstrated that the variation of GHG emissions is significant across the range of practical operational conditions. With system optimization, however, WWTPs have the potential to reduce GHG emissions without raising operating costs or reducing effluent quality. Further research should include a mechanistic examination of processes that produce methane (CH4) in the wastewater treatment stream and nitrous oxide (N2O) in the sludge treatment stream

Oral surgery referrals at a UK dental hospital in the context of a managed vlinical network: a mixed-methods study

Background and aims: To inform the first Welsh OS Managed Clinical Network (MCN), a mixed-methods study investigated existing patterns, quality, suitability and reasons for referral to secondary care at the University Dental Hospital in Wales. Materials and methods: A random sample of 298 OS referrals were studied over a six-month period. Data recording proforma included details on referral practitioner, patient and referral diagnosis. Referrals were categorised by Levels of complexity (Levels 1, 2 and 3) and face-to-face, semi-structured and audio-recorded interviews were conducted with five frequent referrers. Results: The age range of patients was between 1 and 92 years, with over 58% (n=174) female. Majority of referrals (80%) were from GDPs. Top six practices accounted for a fifth (21%) of referrals, with three of these practices were corporate dental chains. Approximately, a third of referrals were categorised as Level 1 (37%), Level 2 (33%) and Level 3 (30%) complexity. 16% provided no medical history, and only 13% included supporting radiographs. Five themes emerged as reasons for oral surgery referrals: contract limitations, perception that new graduates lack OS practical skills, communication, practice resources and risk. Conclusions: Priorities for the Wales OS MCN are to reduce inappropriate referrals to secondary care and to ensure quality referrals. Introduction of the pan-Wales electronic Referral Management System in May 2019 is welcome in this context. The newly formed Health Education and Improvement Wales, with lead roles in education, training and shaping the healthcare workforce, will form a vital part in tackling barriers for safe OS in primary care

ISOGEOMETRIC ANALYSIS AND PATCHWISE REPRODUCING POLYNOMIAL PARTICLE METHOD FOR PLATES

Isogeometric analysis (IGA) ([8, 16, 27]) is designed to combine two tasks, design by Computer Aided Design (CAD) and Finite Element Analysis (FEA), so that it drastically reduces the error in the representation of the computational domain and the re-meshing by the use of “exact” CAD geometry directed at the coarsest level of discretization. This is achieved by using B-splines or non-uniform rational B-splines (NURBS) for the description of geometries as well as for the representation of unknown solution fields. In order to handle the singularities arising in the PDEs, Babu?ska and Oh [7] introduced mapping techniques, called the Method of Auxiliary Mapping (MAM), into conventional p-version of Finite Element Methods (FEM). In a similar spirit to MAM, it is possible to construct a novel NURBS geometrical mapping that generates singular functions resembling the singularities. The proposed mapping technique is concerned with constructions of unconventional novel geometrical mappings by which push-forward of B-spline functions defined on the parameter space generates singular functions in a physical domain that resemble the given point singularities. In other words, the pull-back of the singularity into the parameter space by the non standard NURBS mapping becomes highly smooth. However, the mapping technique is not able to handle in the framework of IGA. Thus, we consider how to use the proposed mapping method in IGA of elliptic prob- lems and elasticity containing singularities without changing the design mapping. For this end, we embed the mapping method into the standard IGA that uses NURBS basis functions for which h - p - k-refinements are applicable for improved computational solution. In other words, the mapping method will be used to enrich NURBS basis functions around neighborhood of singularities so that they can capture singular behaviors of the solution to be approximated. Finally, Reproducing Polynomial Particle Method (RPPM) is one of meshless methods that use meshes minimally or do not use meshes at all. In this disserta- tion, the RPPM is employed for free vibration and buckling of the first order shear deformation model (FSDT), called the Reissner-Mindlin plate, and for analysis of boundary layer of the Reissner-Mindlin plate. For numerical implementation, we use flat-top partition of unity functions, introduced by Oh et al, and patchwise RPPM in which approximation functions have high order polynomial reproducing property and shape functions satisfying the Kronecker delta property. Also, we demonstrate that our method is more effective than other existing methods in dealing with Reissner- Mindlin plates with various material properties and boundary conditions