Requirement for Golgi-Localized PI(4)P in Fusion of COPII Vesicles with Golgi Compartments

The role of specific membrane lipids in transport between endoplasmic reticulum (ER) and Golgi compartments is poorly understood. Using cell-free assays that measure stages in ER-to-Golgi transport, we screened a variety of enzyme inhibitors, lipid-modifying enzymes, and lipid ligands to investigate requirements in yeast. The pleckstrin homology (PH) domain of human Fapp1, which binds phosphatidylinositol-4-phosphate (PI(4)P) specifically, was a strong and specific inhibitor of anterograde transport. Analysis of wild type and mutant PH domain proteins in addition to recombinant versions of the Sac1p phosphoinositide-phosphatase indicated that PI(4)P was required on Golgi membranes for fusion with coat protein complex II (COPII) vesicles. PI(4)P inhibition did not prevent vesicle tethering but significantly reduced formation of soluble n-ethylmaleimide sensitive factor adaptor protein receptor (SNARE) complexes between vesicle and Golgi SNARE proteins. Moreover, semi-intact cell membranes containing elevated levels of the ER-Golgi SNARE proteins and Sly1p were less sensitive to PI(4)P inhibitors. Finally, in vivo analyses of a pik1 mutant strain showed that inhibition of PI(4)P synthesis blocked anterograde transport from the ER to early Golgi compartments. Together, the data presented here indicate that PI(4)P is required for the SNARE-dependent fusion stage of COPII vesicles with the Golgi complex

¿Cuántos Cancioneros de Baena?

This article, drawing upon documents that trace the history of the extant Cancionero de Baena (Bibliothèque national de France, MS. Esp. 37; Dutton PN1) from the library of Isabel I (Segovia, 1503) to those that prove that Jorge de Beteta of Soria gave it to Philip II for the Escorial library in 1575 or 1576 and comparing its contents to works of Gonzalo Argote de Molina and BNE MSS/3788 (Dutton MN15), shows that ca.1580 there were two copies of the Cancionero de Baena in the Escorial library as well as one of a “Cancionero de Alfonso Álvarez de Villasandino,” which was in fact probably a third copy

Optical and near-infrared observations of the microquasar V4641 Sagittarii during the 1999 September outburst

We present photometric and spectroscopic optical and near-infrared (NIR) observations (Based on observations collected at the European Southern Observatory, Chile (ESO ID 63.H-0493 and 64.H-0382)) taken during the outburst of the microquasar V4641 Sgr = SAX J1819.3-2525 (in'tzand et al., 2000) in September 1999. We observed an increase in the J-Ks colour between 5 and 8 days after the outburst, which we interpret as likely evidence for the presence of dust around the source. We also observed an extraordinarily strong, broad and variable H_alpha line, with a velocity width of 4560 km/s suggesting the presence of a high-velocity outflow component. We constrain the distance of the system between 3 and 8 kpc, locating it further away than previously derived from radio observations (Hjellming et al., 2000), but consistent with Orosz et al. (2001). We then discuss the nature of this system, showing that the companion star is either a B3-A2 main sequence star, or a B3-A2 sub-giant crossing the Hertzsprung gap. The system is therefore an Intermediate or High Mass X-ray Binary System (IMXB or HMXB). The distance derived by these optical/NIR observations implies that the jets observed by Hjellming et al. (2000) would then exhibit apparent velocities of ~ 10 c. We finally discuss the possibility of an interaction between the jets and surroundings of the source, and also of this source being a ``microblazar''.Comment: 7 pages, 6 figures, accepted for publication in MNRA

A New List of Cuban Crinoids (Echinodermata: Crinoidea)

This review is based on the most recent taxonomic classification of extant Cuban Crinoidea. Existing material in natural history collections in Cuba was analyzed in detail and accurately reflects species presence. The crinoid literature was also reviewed. Several previously unpublished observations of species found in waters off Cuba have also been included. This paper provides the most up to date description of the Cuban crinoid fauna. Habitat, distribution, depth range and localities in Cuba are reported for each species. Collection codes for available specimens are included when known. The work describes a total of 33 species (plus two subspecies) in 20 genera and 10 families representing three of the four living orders of the Class Crinoidea

Concert recording 2019-04-30

[Track 1]. La pastorella mia / Francesca Caccini -- [Track 2]. Per pietà bell\u27idol mio / Marianne Martines -- [Track 3]. From Sechs Lieder, opus 13. Ich stand in dunklen Träumen [Track 4]. Liebeszauber [Track 5]. Ich hab\u27 in Deinem Auge / Clara Schumann -- [Track 6]. From though love be a day. Thy fingers make early flowers [Track 7]. lily has a rose [Track 8]. maggie and millie and molly and may / Gwyneth Walker -- [Track 9]. From Opus 9. Sehnsucht [Track 10]. Verlust [Track 11]. Die Nonne / Fanny Mendelssohn Handel -- [Track 12]. Ihwau Pear Blossom Rain / Lee Won-ju -- [Track 13]. Cantique [Track 14]. La mer / Nadia Boulanger -- [Track 15]. Three Browning songs, opus 44. I sent my heart up to thee [Track 16]. Ah, love, but a day [Track 17]. The year\u27s at the spring / Amy Beach -- [Track 18]. Beyond all price from The scarlet letter / Lori Laitman

Safe Farm: The Impact of an Iowa Public Information Campaign

The 1992 public information campaign, Safe Farm, made farm safety messages available to a diverse and independent target audience of 104,000 full-time and parttime Iowa farm operators and their families. The print portion of the campaign reached 5.03 million Iowa newspaper subscribers. A series of public service announcements received at least 180 h of air time on more than 100 Iowa radio stations and nearly 80,000 farm safety publications were distributed by Iowa State University (ISU) Extension during the campaign.The impact of this public information campaign was measured by a baseline and follow-up telephone survey of 460 Iowa farm operators. The baseline survey showed that farm operators relied heavily on local media for farm safety information, as well as the cooperative extension service.When asked where they obtained safety information, 95% of the respondents said newspapers and magazines, 82% radio, 77% television, 59% relied on publications from ISU Extension, and 33% relied on ISU Extension staff.The follow-up survey measured significant improvements in Iowa farm operators’ awareness, concern, and behavior based on three indices composed of scales common to both surveys. A multiple regression analysis was conducted based on a causal model. The multivariate test indicated that these changes could not be statistically attributed to the Safe Farm campaign

Magnetic-crystallographic phase diagram of superconducting parent compound Fe1+x_{1+x}Te

hrough neutron diffraction experiments, including spin-polarized measurements, we find a collinear incommensurate spin-density wave with propagation vector $\mathbf k =$ ($0.4481(4) \, \,0 \, \, \frac1 2$) at base temperature in the superconducting parent compound Fe$_{1+x}$Te. This critical concentration of interstitial iron corresponds to $x \approx 12$ and leads crystallographic phase separation at base temperature. The spin-density wave is short-range ordered with a correlation length of 22(3) \AA, and as the ordering temperature is approached its propagation vector decreases linearly in the H-direction and becomes long-range ordered. Upon further populating the interstitial iron site, the spin-density wave gives way to an incommensurate helical ordering with propagation vector $\mathbf k =$ ($0.3855(2) \, \,0 \, \, \frac1 2$) at base temperature. For a sample with $x \approx 9(1)$, we also find an incommensurate spin-density wave that competes with the bicollinear commensurate ordering close to the N\'eel point. The shifting of spectral weight between competing magnetic orderings observed in several samples is supporting evidence for the phase separation being electronic in nature, and hence leads to crystallographic phase separation around the critical interstitial iron concentration of 12%. With results from both powder and single crystal samples, we construct a magnetic-crystallographic phase diagram of Fe$_{1+x}$Te for $ 5% < x <17%

The nature of the infrared counterpart of IGR J19140+0951

The INTEGRAL observatory has been (re-)discovering new X-ray sources since the beginning of nominal operations in early 2003. These sources include X-ray binaries, Active Galactic Nuclei, cataclysmic variables, etc. Amongst the X-ray binaries, the true nature of many of these sources has remained largely elusive, though they seem to make up a population of highly absorbed high-mass X-ray binaries. One of these new sources, IGR J19140+0951, was serendipitously discovered on 2003 Mar 6 during an observation of the galactic microquasar GRS 1915+105. We observed IGR J19140+0951 with UKIRT in order to identify the infrared counterpart. Here we present the H- and K-band spectra. We determined that the companion is a B0.5-type bright supergiant in a wind-fed system, at a distance \la 5 kpc.Comment: 5 pages, 2 figures, accepted for publication in MNRA

Characterization of slow and fast phase nystagmus

A current literature review of the analog and digital process of vestibular and optical kinetic nystagmus reveals little agreement in the methods used by various labs. The strategies for detection of saccade (fast phase velocity component of nystagmus) vary between labs, and most of the process have not been evaluated and validated with a standard database. A survey was made of major vestibular labs in the U.S. that perform computer analyses of vestibular and optokinetic reflexes to stimuli, and a baseline was established from which to standardize data acquisition and analysis programs. The concept of an Error Index was employed as the criterium for evaluating the performance of the vestibular analysis software programs. The performance criterium is based on the detection of saccades and is the average of the percentages of missed detections and false detections. Evaluation of the programs produced results for lateral gaze with saccadic amplitude of one, two, three, five, and ten degrees with various signal-to-noise ratios. In addition, results were obtained for sinusoidal pursuit of 0.05, 0.10, and 0.50 Hz with saccades from one to ten degrees at various signal-to-noise ratios. Selection of the best program was made from the performance in the lateral gaze with three degrees of saccadic amplitude and in the 0.10 Hz sinusoid with three degrees of saccadic amplitude