rDNA internal transcribed spacer sequence analysis of Lycoris Hert.

The interspecific relationships of Lycoris species were studied by internal transcribed spacer (ITS) sequences. ITS fragments of 14 species were amplified, sequenced and analysed. The results showed that ITS sequences of 14 species were different from each other and the ITS lengths of 14 species were about 652 bp. The GC content of ITS2 sequences was bigger than that of ITS1. Clustering results based on ITS sequences showed that Lycoris species could be divided into three clades. The classification was basically consistent with those of karyotype and morphology. This paper suggested that the likelihood of hybrid origin of Lycoris species was supported and ITS could be used as a good molecular marker to identify plants of Lycoris.Keywords: Lycoris Hert., internal transcribed spacer (ITS), molecular taxonomy, interspecific relationshipAfrican Journal of Biotechnology Vol. 11(29), pp. 7361-7365, 10 April, 201

A Locus for Brachydactyly Type A-1 Maps to Chromosome 2q35-q36

Brachydactyly type A-1 (BDA1) was, in 1903, the first recorded example of a human anomaly with Mendelian autosomal dominant inheritance. Two large families, the affected members of which were radiographed, were recruited in the study we describe here. Two-point linkage analysis for pedigree 1 (maximum LOD score [Z(max)] 6.59 at recombination fraction [θ] 0.00) and for pedigree 2 (Z(max)=5.53 at θ=0.00) mapped the locus for BDA1 in the two families to chromosome 2q. Haplotype analysis of pedigree 1 confined the locus for family 1 within an interval of <8.1 cM flanked by markers D2S2248 and D2S360, which was mapped to chromosome 2q35-q36 on the cytogenetic map. Haplotype analysis of pedigree 2 confined the locus for family 2 within an interval of <28.8 cM flanked by markers GATA30E06 and D2S427, which was localized to chromosome 2q35-q37. The two families had no identical haplotype within the defined region, which suggests that the two families were not related

Mutations in IHH, encoding Indian hedgehog, cause brachydactyly type A-1

Brachydactyly type A-1 (BDA-1; MIM 112500) is characterized by shortening or missing of the middle phalanges (Fig. 1a). It was first identified by Farabee in 1903 (ref. 2), is the first recorded example of a human anomaly with Mendelian autosomal-dominant inheritance and, as such, is cited in most genetic and biological textbooks. Here we show that mutations in IHH, which encodes Indian hedgehog, cause BDA-1. We have identified three heterozygous missense mutations in the region encoding the amino-terminal signaling domain in all affected members of three large, unrelated families. The three mutant amino acids, which are conserved across all vertebrates and invertebrates studied so far, are predicted to be adjacent on the surface of IHH