Association between high dietary intake of the n-3 polyunsaturated fatty acid docosahexaenoic acid and reduced risk of Crohn's disease

Background: There are plausible mechanisms for how dietary docosahexaenoic acid (DHA), an n−3 polyunsaturated fatty acid, could prevent Crohn's disease (CD). Aim: To conduct a prospective study to investigate the association between increased intake of DHA and risk of CD. Methods: Overall, 229 702 participants were recruited from nine European centres between 1991 and 1998. At recruitment, dietary intakes of DHA and fatty acids were measured using validated food frequency questionnaires. The cohort was monitored through to June 2004 to identify participants who developed incident CD. In a nested case–control analysis, each case was matched with four controls; odds ratios (ORs) were calculated for quintiles of DHA intake, adjusted for total energy intake, smoking, other dietary fatty acids, dietary vitamin D and body mass index. Results: Seventy-three participants developed incident CD. All higher quintiles of DHA intake were inversely associated with development of CD; the highest quintile had the greatest effect size (OR = 0.07; 95% CI = 0.02–0.81). The OR trend across quintiles of DHA was 0.54 (95% CI = 0.30–0.99, Ptrend = 0.04). Including BMI in the multivariate analysis, due to its correlation with dietary fat showed similar associations. There were no associations with the other dietary fatty acids studied. Conclusion: There were inverse associations, with a biological gradient between increasing dietary docosahexaenoic acid intakes and incident Crohn's disease. Further studies in other populations should measure docosahexaenoic acid to determine if the association is consistent and the hypothesis tested in randomised controlled trials of purely docosahexaenoic acid supplementation

In vivo articular cartilage deformation: noninvasive quantification of intratissue strain during joint contact in the human knee

The in vivo measurement of articular cartilage deformation is essential to understand how mechanical forces distribute throughout the healthy tissue and change over time in the pathologic joint. Displacements or strain may serve as a functional imaging biomarker for healthy, diseased, and repaired tissues, but unfortunately intratissue cartilage deformation in vivo is largely unknown. Here, we directly quantified for the first time deformation patterns through the thickness of tibiofemoral articular cartilage in healthy human volunteers. Magnetic resonance imaging acquisitions were synchronized with physiologically relevant compressive loading and used to visualize and measure regional displacement and strain of tibiofemoral articular cartilage in a sagittal plane. We found that compression (of 1/2 body weight) applied at the foot produced a sliding, rigid-body displacement at the tibiofemoral cartilage interface, that loading generated subject- and gender-specific and regionally complex patterns of intratissue strains, and that dominant cartilage strains (approaching 12%) were in shear. Maximum principle and shear strain measures in the tibia were correlated with body mass index. Our MRI-based approach may accelerate the development of regenerative therapies for diseased or damaged cartilage, which is currently limited by the lack of reliable in vivo methods for noninvasive assessment of functional changes following treatment

The Sachs-Wolfe Effect: Gauge Independence and a General Expression

In this paper we address two points concerning the Sachs-Wolfe effect: (i) the gauge independence of the observable temperature anisotropy, and (ii) a gauge-invariant expression of the effect considering the most general situation of hydrodynamic perturbations. The first result follows because the gauge transformation of the temperature fluctuation at the observation event only contributes to the isotropic temperature change which, in practice, is absorbed into the definition of the background temperature. Thus, we proceed without fixing the gauge condition, and express the Sachs-Wolfe effect using the gauge-invariant variables.Comment: 5 pages, closer to published versio

Host Genetics Predict Clinical Deterioration in HCV-Related Cirrhosis

Single nucleotide polymorphisms (SNPs) in the epidermal growth factor (EGF, rs4444903), patatin-like phospholipase domain-containing protein 3 (PNPLA3, rs738409) genes, and near the interleukin-28B (IL28B, rs12979860) gene are linked to treatment response, fibrosis, and hepatocellular carcinoma (HCC) in chronic hepatitis C. Whether these SNPs independently or in combination predict clinical deterioration in hepatitis C virus (HCV)-related cirrhosis is unknown. We genotyped SNPs in EGF, PNPLA3, and IL28B from liver tissue from 169 patients with biopsy-proven HCV cirrhosis. We estimated risk of clinical deterioration, defined as development of ascites, encephalopathy, variceal hemorrhage, HCC, or liver-related death using Cox proportional hazards modeling. During a median follow-up of 6.6 years, 66 of 169 patients experienced clinical deterioration. EGF non-AA, PNPLA3 non-CC, and IL28B non-CC genotypes were each associated with increased risk of clinical deterioration in age, sex, and race-adjusted analysis. Only EGF non-AA genotype was independently associated with increased risk of clinical deterioration (hazard ratio [HR] 2.87; 95% confidence interval [CI] 1.31–6.25) after additionally adjusting for bilirubin, albumin, and platelets. Compared to subjects who had 0–1 unfavorable genotypes, the HR for clinical deterioration was 1.79 (95%CI 0.96–3.35) for 2 unfavorable genotypes and 4.03 (95%CI 2.13–7.62) for unfavorable genotypes for all three loci (Ptrend<0.0001). In conclusion, among HCV cirrhotics, EGF non-AA genotype is independently associated with increased risk for clinical deterioration. Specific PNPLA3 and IL28B genotypes also appear to be associated with clinical deterioration. These SNPs have potential to identify patients with HCV-related cirrhosis who require more intensive monitoring for decompensation or future therapies preventing disease progression

Computational design of syntheses leading to compound libraries or isotopically labelled targets

Although computer programs for retrosynthetic planning have shown improved and in some cases quite satisfactory performance in designing routes leading to specific, individual targets, no algorithms capable of planning syntheses of entire target libraries - important in modern drug discovery - have yet been reported. This study describes how network-search routines underlying existing retrosynthetic programs can be adapted and extended to multi-target design operating on one common search graph, benefitting from the use of common intermediates and reducing the overall synthetic cost. Implementation in the Chematica platform illustrates the usefulness of such algorithms in the syntheses of either (i) all members of a user-defined library, or (ii) the most synthetically accessible members of this library. In the latter case, algorithms are also readily adapted to the identification of the most facile syntheses of isotopically labelled targets. These examples are industrially relevant in the context of hitto-lead optimization and syntheses of isotopomers of various bioactive molecules

Age-Related Attenuation of Dominant Hand Superiority

The decline of motor performance of the human hand-arm system with age is well-documented. While dominant hand performance is superior to that of the non-dominant hand in young individuals, little is known of possible age-related changes in hand dominance. We investigated age-related alterations of hand dominance in 20 to 90 year old subjects. All subjects were unambiguously right-handed according to the Edinburgh Handedness Inventory. In Experiment 1, motor performance for aiming, postural tremor, precision of arm-hand movement, speed of arm-hand movement, and wrist-finger speed tasks were tested. In Experiment 2, accelerometer-sensors were used to obtain objective records of hand use in everyday activities

On the Top-Weight Rational Cohomology of Ag\mathcal{A}_g

We compute the top-weight rational cohomology of $\mathcal{A}_g$ for $g=5$, $6$, and $7$, and we give some vanishing results for the top-weight rational cohomology of $\mathcal{A}_8, \mathcal{A}_9,$ and $\mathcal{A}_{10}$. When $g=5$ and $g=7$, we exhibit nonzero cohomology groups of $\mathcal{A}_g$ in odd degree, thus answering a question highlighted by Grushevsky. Our methods develop the relationship between the top-weight cohomology of $\mathcal{A}_g$ and the homology of the link of the moduli space of principally polarized tropical abelian varieties of rank $g$. To compute the latter we use the Voronoi complexes used by Elbaz-Vincent-Gangl-Soul\'e. Our computations give natural candidates for compactly supported cohomology classes of $\mathcal{A}_g$ in weight $0$ that produce the stable cohomology classes of the Satake compactification of $\mathcal{A}_g$ in weight $0$, under the Gysin spectral sequence for the latter space.Comment: 33 pages, 3 figure

Reactivity of polar organometallic compounds in unconventional reaction media : challenges and opportunities

Developing new green solvents in designing chemical products and processes or successfully employing the already existing ones is one of the key subjects in green chemistry and is especially important in organometallic chemistry, which is an interdisciplinary field. Can we advantageously also use unconventional reaction media in place of current harsh organic solvents for polar organometallic compounds? This microreview critically analyses the state of the art with regard to this topic and showcases recent developments and breakthroughs that are becoming new research directions in this field. Because metals cover a vast swath of the Periodic Table the content is organised into three sections discussing the reactivity of organometallic compounds of s-, p- and d-block elements in unconventional solvents

Intervertebral Disc Internal Deformation Measured by Displacements Under Applied Loading with MRI at 3T

Purpose: Noninvasive assessment of tissue mechanical behavior could enable insights into tissue function in healthy and diseased conditions and permit the development of effective tissue repair treatments. Measurement of displacements under applied loading with MRI (dualMRI) has the potential for such biomechanical characterization on a clinical MRI system. Methods: dualMRI was translated from high-field research systems to a 3T clinical system. Precision was calculated using repeated tests of a silicone phantom. dualMRI was demonstrated by visualizing displacements and strains in an intervertebral disc and compared to T 2 measured during cyclic loading. Results: The displacement and strain precisions were 24 mm and 0.3% strain, respectively, under the imaging parameters used in this study. Displacements and strains were measured within the intervertebral disc, but no correlations were found with the T 2 values. Conclusion: The translation of dualMRI to a 3T system unveils the potential for in vivo studies in a myriad of tissue and organ systems. Because of the importance of mechanical behavior to the function of a variety of tissues, it&apos;s expected that dual-MRI implemented on a clinical system will be a powerful tool in assessing the interlinked roles of structure, mechanics, and function in both healthy and diseased tissues. Key words: displacement; strain; intervertebral disc; elastography; clinical magnetic resonance imaging The mechanical behavior of a biomaterial is intimately linked to its function, both within natural tissues and biocompatible materials. The material properties and also the loading environment affect the mechanical behavior of tissues, particularly as they relate to healthy and pathological conditions within the body. Noninvasive methods to quantify the mechanical behavior of biomaterials and tissues would permit the measurement of material properties while both preserving the native loading environment and accounting for its influence. Such methods would improve longitudinal studies in animals or humans, greatly reducing the number of animals or human subjects needed for clinical trials, among other benefits. Additionally, these methods could also be implemented to assess tissue quality in patients and monitor the long-term effectiveness of pharmacological or tissue engineering treatments. Some studies have attempted to correlate various relaxometry measures, such as T 2 and T 1r , to mechanical properties in a number of tissues (1,2), motivated by the idea that relaxometry measures correspond to biochemistry and, in turn, are related to mechanical behavior. However, these correlations are based on mechanical tests performed on explanted tissue and do not take advantage of the noninvasive nature of MRI to fully assess tissues within their native environment. Because techniques like displacementencoded MRI can measure displacements and strains noninvasively (3), such a technique for biomechanical characterization of tissue could complement other quantitative MRI techniques (4) for the overall assessment of soft tissue function. The goal of this study was to translate a noninvasive imaging technique that can measure displacements under applied loading with MRI (dualMRI) to a clinical 3T MRI system. Previous efforts in our lab have utilized dualMRI on a high-field MRI scanner, where displacements and strains were measured in explants of healthy cartilage (3) and in intact joints, including tibiofemoral cartilage (5) and degenerated intervertebral discs in animal models (6). Despite the advantages proffered by the higher magnetic field of research MRI systems, these systems are often limited to small volumes and are not approved for clinical use on human subjects. Translation of the dualMRI technique to a clinical scanner would allow for studies of live animals and human subjects, as well as larger tissues and biomaterials that would not be able to fit into the limited confines of a small-bore high-field MRI system. The technical challenge of implementing dualMRI on a living animal or human subject is twofold: (1) the translation of a load-synchronized, displacement-encoded MRI sequence and (2) the development of noninvasive, organ-or joint-specific loading systems capable of repeatable cyclic loading of the tissues of interest. In this article, to address the first of these challenges, we report the translation of dualMRI to a clinical MRI system at 3T, the determination of technique precision using imaging phantoms, and the demonstration of this technique within an intact human intervertebral disc section. We also demonstrate that the displacements and strains measured with dualMRI can be compared to the T 2 distribution of the disc measured under cyclic load. 1231 This research represents a critical step toward the noninvasive in vivo measurement of biomaterial mechanical properties, permitting future studies in clinical and animal research. METHODS Displacement-Encoded Imaging dualMRI was implemented on a 3T clinical MRI system (General Electric Signa HDx, Waukesha, WI) using an eight-channel knee volume coil and a custom pulse sequence After complementary phase-cycled scans were combined for CANSEL (8), a phase difference reconstruction across all channels (9) was used to isolate the overall phase difference between the reference and encoded scans. A sum of squares magnitude reconstruction across all channels was used to compute the signal-to-noise ratio (SNR) of a region-of-interest (ROI) with respect to a region of noise (i.e., air). The displacement in the encoding direction was then computed as a function of the phase difference, as previously described (3,10). Orthogonal in-plane displacements were then smoothed with a Gaussian 5 Â 5 kernel (11) prior to estimation of the Green-Lagrange strain tensor (12). Precision of dualMRI The precision of our dualMRI technique was evaluated across five repeated scan series of a silicone gel phantom (Sylgard 527, Dow Corning, Elizabethtown, KY) that was cyclically loaded with an MRI-compatible loading apparatus (4). Displacement encoding was accomplished with an encoding gradient area of 3.9145 mT ms/mm, equivalent to displacement encoding of 0.33 p/mm, based on pilot studies that showed no more than 63 mm of expected internal deformation. A mixing time (TM) of 600 ms, which included spoiler gradients immediately before acquisition, allowed for the onset of loading prior to data acquisition during the load plateau. TM is limited by the capabilities of the load apparatus (i.e., time required to reach desired load); therefore, the minimum TM allowable by the loading system was chosen to maximize the stimulated echo signal. SSFSE acquisition parameters were as follows: effective echo time (TE) ¼ 62 ms, matrix size ¼ 256 Â 256, spatial resolution ¼ 703 Â 703 mm 2 , and eight averages. The SSFSE sequence was limited by the system software to a field of view of 180 Â 180 mm 2 and slice thickness of 3 mm. Because the acquisition and the loading were synchronized, the effective repetition time was dictated by the 3-s duration loading cycles, during which a load of 18.5 N was applied for 1.5 s, based on previous studies (4,6). The experiment time for all scans within each repeated dualMRI experiment was 10 min. Image acquisition was completed before the load was released within each cycle. Imaging data was processed as described above to calculate in-plane displacements. Precision was computed as the pooled standard deviation across the five repeated scan series of displacement and strain values across 16 points within the imaging phantom (3,5). Precision was calculated for raw displacements and displacements and strains after 10-100 cycles of smoothing (11). dualMRI in an Intact Intervertebral Disc Section To demonstrate the dualMRI technique in a biological specimen, cyclic compression and imaging of a cadaveric intervertebral disc section was synchronized. A cadaveric lumbar spine was obtained from a tissue bank (AlloSource, Centennial, CO) and kept frozen until specimen preparation. The donor was a 22-year-old male and weighed 111 kg, with no medical history of disc degeneration or other spine disease. A spine segment inclusive of the fourth lumbar vertebra (L4) to the fifth lumbar Timing of dualMRI on a 3T MRI system. This schematic shows the timing events for dualMRI in a clinical 3T MRI system (a). Cyclic loading was synchronized with radiofrequency (RF) pulses and gradient actions, including the gradient applied for displacement encoding (G de ). Gradient actions in the readout, phase-encode, and slice-select directions are typical for a singleshot fast spin echo (SSFSE) acquisition and, for brevity, are not shown here. RF transmission pulses are shown in the solid lines and receive action (i.e. acquisition) is shown in dashed lines. Standard MRI scans of an undeformed and deformed imaging phantom (b) are also shown to demonstrate the time points during which the key dualMRI actions were performed. The undeformed image was acquired during the equivalent time point when the phase-cycled RF pulses and displacement encoding gradient, with gradient strength k, were applied. After a mixing time (TM), dualMRI data was acquired using SSFSE during the deformed state. 1232 Chan and Neu vertebra (L5) was excised. The spinal processes and facet joints were removed by transecting the pedicles of the vertebral arch, retaining the vertebral bodies superior and inferior to the L4/L5 intervertebral disc. The L4 and L5 vertebral bodies were partially embedded in polymethylmethacrylate, permitting the full disc segment to be anchored into an MRI-compatible loading apparatus for cyclic compression. Prior to cyclic loading, standard MRI was performed to obtain morphological data. Compression of 450 N was then applied with the MRI-compatible cyclic loading apparatus for 1.5 s during a 3-s cycle. Preconditioning of more than 500 loading cycles (based on pilot studies) was applied prior to dualMRI to ensure that the specimen reached a quasi-steady state deformation response to the cyclic loading (13). Displacement-encoded images were acquired during the load plateau period under quasi-steady state. Parameters for dualMRI were same as for the precision studies above. Displacements within the L4/L5 disc were smoothed to 100 smoothing cycles, and strains were computed. During cyclic loading, standard MRI images were also acquired in the undeformed and deformed configurations for the measurement of nominal change in height and width under cyclic compression. The total experiment time for all dualMRI scans was 20 min, excluding the time necessary for the specimen to achieve quasi-steady state (less than 20 min under this experimental loading regime). T 2 Analysis In addition, to investigate whether any correspondence existed between the presence of mechanical loading and mechanical behavior and the measured T 2 , a known correlate to disc biochemistry (14) and stage of degeneration (15), we estimated T 2 prior to cyclic loading, during cyclic loading immediately after dualMRI, and immediately after the cessation of cyclic loading. T 2 was estimated using a set of fast spin echo acquisitions with variable TE (TEs ¼ 20, 60, 100, 140, 180, and 240 ms) of the same imaging plane as dualMRI. Repetition time was set to 3000 ms, which is approximately four to five times T 1 of human disc (16), to minimize T 1 -weighting. An electronic trigger was used to synchronize the acquisitions for T 2 mapping under cyclic loading. T 2 mapping experiments were performed within 10 min under each loading condition. A least squares fit of an exponential decay curve was used to estimate T 2 at each pixel within the disc ROI before, during and after cyclic loading. Histograms of the T 2 values in each ROI were generated using bins of 5 ms intervals for qualitative comparison. The ROIs were also divided into five regions along the width of the disc, and, separately, into five regions along the height of the disc. Average regional T 2 was computed and compared for each of these sections between loading conditions. Average T 2 values are reported as mean 6 standard deviation. T 2 values were correlated pixel-by-pixel to displacements and strains using linear regression. Statistical significance was defined as P &lt; 0.05. RESULTS dualMRI Precision Displacements measured by dualMRI, displacements after smoothing, and estimated strains were visualized in the silicone phantom FIG. 2. Displacement and strains in a silicone phantom used to evaluate precision. A cyclically loaded silicone phantom was used to validate displacements (a) and strains (b), and determine relationships between displacement and strain precision (c). Including raw displacements, and displacements and strains after 10 and 100 smoothing cycles, depict the influence of displacement smoothing on deformation patterns. Displacements and strains were measured with dualMRI under the same loading conditions that resulted in the undeformed and deformed images in Disc Displacements and Strains at 3T with DualMRI 1233 Intervertebral Disc Displacements and Strains dualMRI on a 3T MRI system permitted the visualization of displacements and strains within the L4/L5 intervertebral disc T 2 Values The average T 2 values across the full disc before, during, and after cyclic loading were 76.8 6 33.7, 80.8 6 33.8, and 80.5 6 34.7 ms, respectively DISCUSSION Translation of dualMRI from research (9.4 T/30 cm bore) to clinical (3.0 T/60 cm bore) MRI systems presented several technical challenges associated with the larger imaging volume and weaker magnetic field, including reduced spatial resolution and faster T 1 decay of the displacement-encoded signal. Despite a reduction in spatial resolution and increased slice thickness, the precision of raw displacement measurement, 95 mm, fell within the ranges of raw displacement precisions measured previously on a research MRI system (4,5). This is most likely because the precision of displacement is tied closely to the SNR (4), which is increased with larger voxel sizes. The displacement and strain fields and precision values after smoothing were also comparable to previous studies 1234 Chan and Neu note that increasing the amount of smoothing could increase bias between the smoothed value and the true value, previous studies with displacement smoothing showed that this bias is often smaller than the precision (11). This validation study therefore shows that, if larger voxel sizes are acceptable for the tissue of interest, displacement precisions of better than half the spatial resolution can be achieved without smoothing, and smoothing can improve displacement and strain precisions up to 24 mm and 0.3%, respectively. Although some spatial resolution may be lost in the translation from research to clinical scanner, the ability to measure displacements and strains noninvasively in larger volumes and potentially living subjects far outweigh the disadvantages of the clinical system. Several of the imaging parameters used in this study were limited by the imaging system, loading device, or image processing software. The effect on SNR by T 2 decay with TE (in fast spin echo acquisitions) and by T 1 decay with TM (in stimulated echo applications) are well known physical phenomena (3,4,10). Accordingly, image acquisition parameters were chosen to minimize TE and TM within the limitations of the chosen acquisition size and the timing of the load system, respectively. The displacement encoding of 0.33 p/mm was selected to avoid excessive phase wrapping and ease computational efforts in unwrapping the phase. Based on pilot studies, we expected internal deformations of no more than 3 mm in absolute value and therefore set the displacement encoding to encode 3 mm as p. Spatial resolution in this study was limited by the field of view and the choice in acquisition matrix size. In preliminary studies, we compared displacement precision for square matrix sizes of 256 Â 256, 384 Â 384, and 512 Â 512. The additional lines of acquisition resulted in longer effective TEs and required longer load plateaus to accommodate the longer total acquisition time, resulting in attenuation of signal and loss of precision. Although a smaller matrix size (i.e., 128 Â 128) could have been chosen for higher SNR and potentially improved FIG. 4. T 2 analysis of a cyclically loaded intervertebral disc. T 2 maps were estimated for an intervertebral disc (a), before (blue), during (red), and after (green) cyclic loading in the same imaging slice as was used for dualMRI. ROIs (mask for disc before loading shown) were divided into five regions along the width (b) and the height (c) and used to compare average regional T 2 and a histogram of T 2 values (binned in 5-ms intervals) across the full disc (d). Although no displacements and strains were significantly correlated to T 2 values (P &gt; 0.05), the relationship with E xy after 100 cycles of smoothing for each pixel within the ROI is shown (e) because it had the highest R 2 of all linear regressions. Disc Displacements and Strains at 3T with DualMRI 1235 displacement precision, we desired a spatial resolution of at least 1 mm and therefore chose 256 Â 256 acquisition matrix. Although the aforementioned parameters are limited by the current system, a detailed, parameterized study of the effects of TE, TM, displacement encoding strength, spatial resolution, and other imaging parameters could be useful in further characterizing this technique. In this study, dualMRI was demonstrated on the clinical MRI system with a clinically relevant human intervertebral disc segment In previous studies, texture correlation has also been used with magnitude-based MRI to measure the internal strain of intervertebral discs (18). This previous study reported a displacement resolution of 1/20th of a pixel or 12 mm after interpolation and smoothing; however, the optimal subset of 61 pixels, equivalent to 5.2 mm in their study, reportedly resulted in loss of local strain detail. Compared to the cyclic loading conditions used in this study for dualMRI, the texture correlation study used a statically loaded disc. Although texture correlation can provide accurate strain measures in tissues that provide enough SNR and texture in magnitude images, phase-contrast techniques, including dualMRI, do not depend on image texture, which is especially an advantage in tissues that tend toward spatial homogeneity in MRI signal intensity. In this study, we also measured T 2 before, during, and after cyclic loading with the goal of directly comparing T 2 and displacements and strains during cyclic loading Although this study presents promising results for displacement and strain precision, there are a number of improvements that can expedite implementation in vivo. The use of surface array coils should be considered for tissues that would be inappropriate for a volume coil, with SNR as a key deciding factor. Although SSFSE is a fast sequence, it is limited in terms of spatial resolution and relies on the assumption of k-space symmetry to &quot;fill&quot; the remainder of k-space in post processing. A number of acquisition sequences could be substituted for SSFSE, although each acquisition technique presents its own advantages and disadvantages. Additionally, as a technique that synchronizes displacement-encoded MRI with externally applied loading, dualMRI requires an MRI-compatible loading system that can consistently load the tissue or tissues of interest in the confines of a clinical MRI system. The loading system should be able to bring the tissue of interest to a quasi-steady state load-deformation response (13) to achieve optimal image quality. Mechanically loading parts of a living animal or human volunteer, without inducing unwanted motion artifacts or causing undue discomfort, poses a strong technical challenge. Although there are a number of developments that remain to be surmounted, this study nonetheless demonstrates for the first time the use of dualMRI on a clinical MRI system, establishing the in vivo potential. CONCLUSIONS dualMRI was translated to a clinical 3T MRI system for the precise measurement of internal tissue deformation. The precision of displacement measurements was below half the spatial resolution of the image acquisition. Strain precisions were below 2% after just 10 smoothing cycles, and more smoothing (i.e., 100 smoothing cycles) permitted strain precisions of 0.3%. The sequence was demonstrated with a controlled loading system, permitting the visualization of displacement and strain fields in a cyclically compressed intervertebral disc. T 2 mapping during cyclic loading also provided an indication of the structural and biochemical characteristics of the disc. A combination of dualMRI and quantitative MRI could 1236 Chan and Neu be used in combination to gain insight into the structure and mechanical function of tissue noninvasively. ACKNOWLEDGMENTS The authors gratefully acknowledge the assistance of Kent Butz and Paull Gossett, in the specimen preparation and the design of the loading apparatus, Aaditya Chandramouli for his assistance in specimen preparation, Gregory Tamer for his technical assistance on the clinical MRI system, and Eric Nauman for advice and access to the cadaveric specimen. The purpose of this study was to compare displacement behavior of cyclically loaded cadaveric human intervertebral discs as measured noninvasively on a clinical 3.0 T and a research 9.4 T MRI system. Intervertebral discs were cyclically compressed at physiologically relevant levels with the same MRIcompatible loading device in the clinical and research systems. Displacement-encoded imaging was synchronized t