44 research outputs found

    A comprehensive guide to the Argentinian case-bearer beetle fauna (Coleoptera: Chrysomelidae: Camptosomata)

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    Knowledge of Argentinian Camptosomata has largely remained static for the last 60 years since the last publication by Francisco de Asis Monrós in the 1950’s. One hundred and ninety Camptosomata species (182 Cryptocephalinae and 8 Lamprosomatinae) in 31 genera are recorded herein from Argentina. Illustrated diagnostic keys to the subfamilies, tribes, subtribes and genera of Argentinian Camptosomata, plus species checklists and illustrations for all genera of camptosomatan beetles cited for each political region of Argentina are provided. General notes on the taxonomy and distribution, as well as basic statistics, are also included. This study provides basic information about the Camptosomata fauna in Argentina that will facilitate in the accurate generic-level identification of this group and aid subsequent taxonomic revisions, and phylogenetic, ecological, and biogeographic studies. This information will also facilitate faunistic comparisons between neighboring countries. Two nomenclatural acts are proposed: Temnodachrys (Temnodachrys) argentina (Guérin, 1952), comb. n., and Metallactus bivitticollis (Jacoby, 1907), comb. n. The following are new records for Argentina: Stegnocephala xanthopyga (Suffrian, 1863) and Lamprosoma azureum Germar, 1824. Currently, the most diverse camptosomate tribe in Argentina is Clytrini, with almost twice the number of species of Cryptocephalini. New records for Argentina are predicted.Fil: Agrain, Federico Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Chamorro, María Lourdes. National Museum of Natural History; Estados UnidosFil: Cabrera, Nora Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sassi, Davide. Università degli Studi di Milano; ItaliaFil: Roig, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentin

    ORGANIC FARMING ENHANCES THE RECOVERY OF ANCIENT CROPS AND SEGETAL WEEDS IN CATALONIA (NE OF SPAIN)

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    The Area of Natural Interest of Gallecs (ANIG) is one of the largest areas of arable land managed organically in Catalonia. In 2005 a project was launched to convert fields to organic farming and recuperate traditional crop varieties. The aim of this work is to analyse the changes in diversity of the weed flora, including segetal and rare species, and of crops in 20 fields (ca. 50 ha) after the conversion to organic farming during 5 cropping periods (2005-2010). Number of cultivated crops has increased from 1 to 17 including ancient and local varieties of cereal and legumes. Weed biodiversity increased by a 50% in five years at field and regional scale. Crop rotations and sowing organic seeds from other farmers can contribute to the increase and the conservation of the weed diversity in the arable agroecosystems, including segetal species which are currently rare or even have disappeared from most of the conventional arable fields

    Efecto de los tratamientos tecnológicos en la digestión gastrointestinal in vitro de las proteínas bioactivas de la leche de vaca

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    La leche es una fuente de proteínas con alto valor nutricional y algunas de ellas con actividades biológicas. Estas proteínas bioactivas son esenciales para el desarrollo del recién nacido y también pueden utilizarse como ingredientes de alimentos especiales. Así, algunas proteínas de la leche tienen una función antimicrobiana, como la lactoferrina y las inmunoglobulinas del lactosuero, y la lactadherina y la mucina de la membrana del glóbulo graso. Estas proteínas se utilizan para suplementar las leches de fórmula, con el fin de fortalecer las defensas del bebé frente a algunas bacterias patógenas como Cronobacter sakazakii. Se trata de un patógeno emergente que preocupa a la salud pública, puesto que puede producir infecciones en niños y personas inmunodeprimidas. Los principales casos notificados de infecciones causadas por C. sakazakii en lactantes se han relacionado con el consumo de leches de fórmula en polvo contaminadas, que aunque tienen un tratamiento térmico no son estériles. Por ello, es necesario estudiar el efecto de los tratamientos tecnológicos y de la digestión gastrointestinal en la actividad antibacteriana de las proteínas lácteas defensivas.<br /

    Caracterización de la actividad biológica de proteínas y péptidos derivados de subproductos lácteos

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    La leche contiene compuestos que poseen propiedades bioactivas. Entre ellas, cabe destacar la actividad antimicrobiana de la lactoferrina, la lactadherina y el glicomacropéptido (GMP). El uso de estos compuestos como ingredientes en fórmulas infantiles es de gran interés, ya que podrían disminuir el riesgo de infección por Cronobacter sakazakii, un patógeno emergente que causa infecciones en neonatos prematuros. El objetivo de este Trabajo Fin de Máster ha sido el aislamiento y caracterización de proteínas y péptidos bioactivos derivados de subproductos lácteos. Para ello, la lactadherina y el GMP se han aislado a partir de una fracción enriquecida en membrana del glóbulo graso y del lactosuero, respectivamente. Se ha evaluado la actividad antimicrobiana frente a C. sakazakii en fase exponencial y estacionaria. Asimismo, se ha analizado la viabilidad de las células Caco-2/TC7, como modelo de epitelio intestinal, la inhibición de la adhesión e internalización del patógeno a las células y la expresión de los receptores intestinales TLR2, TLR4 y TLR9. El GMP, a las concentraciones de 1,5 y 3 mg/mL, provoca una reducción del crecimiento de C. sakazakii en fase estacionaria tras 24 h de incubación. Por otro lado, la lactoferrina no afecta a la viabilidad de las células Caco-2/TC7. Además, a las concentraciones de 0,5; 1; 2 y 5 mg/mL, la lactoferrina parece inhibir la adhesión e internalización de C. sakazakii a las células y a 10 mg/mL, parece reducir la expresión de los receptores TLR4 y TLR9 con y sin patógeno. Estos resultados conducen a futuros estudios para comprender mejor la actividad antimicrobiana de las proteínas lácteas sobre C. sakazakii.<br /

    Design of a tourist route for the development of the Natabuela People and the Antonio Ante Canton, Imbabura

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    Este trabajo propone el turismo cultural como una nueva alternativa excelsa para la Parroquia Natabuela y el Cantón Antonio Ante, los cuales, por mucho tiempo, han vivido del turismo de compras que se genera con los textiles, olvidando que si un lugar puede ofrecer un turismo mixto podría llegar a abarcar varios tipos de nichos. La investigación se enmarca dentro de los aspectos histórico-culturales de los pueblos de la región en la línea de investigación; con una modalidad cualitativa–cuantitativa, adentrándose en la investigación etnográfica y de campo. Se realizó un análisis de las entrevistas y encuestas aplicadas para una sustentación cuantitativa. Como resultado, se obtuvo la ruta turística descrita cuyo conocimiento contribuye al desarrollo y generación de recursos económicos en el sector y el interés en mantener su cultura.This work proposes cultural tourism as a new excellent alternative for Natabuela Parish and Antonio Ante Canton. For a long time, we have lived off the shopping tourism generated by textiles, forgetting that a place that can offer mixed tourism could cover several types of niches. The research is framed within the historical-cultural aspects of the region's peoples in the line of research; with a qualitative-quantitative modality, entering into ethnographic and field research. An analysis of the interviews and surveys applied was carried out for quantitative support. As a result, the described tourist route was obtained, which contributes to the development and generation of economic resources in the sector and the interest in maintaining its culture

    Educación Ambiental para el Desarrollo de Capacidades en Comunidades Rurales del Parque Nacional Pico de Orizaba

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    El Área Natural Protegida Parque Nacional Pico de Orizaba, tiene una superficie de 19, 750 ha, es la montaña con mayor altitud en México con 5, 636 msnm. Brinda valiosos servicios ambientales, es una importante área de captación para la recarga de acuíferos, mantiene la red hidrológica superficial de varias cuencas, abasteciendo de agua a más de un millón de personas resguardándolas además, ante fenómenos climáticos extremos. Conservar los bosques de coníferas y páramos de altura de esta zona, contribuye a conservar el único glaciar   presente en la montaña. A pesar de ser importante por los servicios que provee, actualmente enfrenta diferentes problemáticas ambientales (caza de fauna silvestre, tala e incendios forestales, por mencionar algunos), que merman las acciones de conservación implementadas. La pérdida de biodiversidad debido principalmente a las actividades realizadas por las comunidades rurales con un alto grado de marginación que se encuentran colindantes, han afectado a especies de gran importancia. En el presente trabajo se aplicaron diferentes estrategias de Educación Ambiental como: capacitaciones, gestión de proyectos, vinculación con otros sectores y con otras comunidades, fortaleciendo las acciones de conservación. Las estrategias se implementaron en dos comunidades: Puerto Nacional en el municipio de Tlachichuca del estado de Puebla y Rancho Nuevo en el municipio de La Perla del estado de Veracruz. Se propició el desarrollo de capacidades de las comunidades, mediante la aplicación de cinco capacitaciones abordando diferentes temas y la creación de grupos de trabajo dando pauta a alternativas laborales que permitan la conservación de la diversidad biológica del parque

    One-step Sustainable Preparation of Superparamagnetic Iron Oxide Nanoparticles Supported on Mesoporous SiO2

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    Superparamagnetic iron oxide nanoparticles (SPIONs) supported on high surface area mesoporous SiO2 are advanced materials of great interest in catalysis, adsorption and biomedicine. Here we present a new process to prepare SPION/SiO2 materials by the impregnation and insitu decomposition of Fe(NO3)3.9H2O on mesoporous SiO2 supports in a 25-50% mol ethanol + CO2 mixture at 523 K and 25.0 MPa. -Fe2O3 nanoparticles (NPs) of average size between 6-9 nm were distributed homogeneously on the supports. NPs deposited into the SBA-15 mesopores but mostly on the external surface of MCM-41. Materials prepared with the highest ethanol content were very homogeneous. Magnetic measurements confirmed the superparamagnetic nature of the materials at room temperature. The process proposed is sustainable and scalable, avoids tedious preparations and the additional high temperature treatment under a controlled atmosphere, as the metal decomposition is performed insitu in the CO2-expanded liquid mixture

    PPAR-γ Gene Expression in Human Adipose Tissue Is Associated with Weight Loss After Sleeve Gastrectomy

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    [EN] Background The peroxisome proliferator-activated receptor (PPAR)-γ plays a key role in adipose tissue differentiation and fat metabolism. However, it is unclear which factors may regulate its expression and whether obese patients have changes in adipose tissue expression of PPAR-γor potential regulators such as miR-27. Thus, our aims were to analyze PPAR-γ and miR-27 expression in adipose tissue of obese patients, and to correlate their levels with clinical variables. Subjects and Methods. We included 43 morbidly obese subjects who underwent sleeve gastrectomy (31 of them completed 1-year follow-up) and 19 non-obese subjects. mRNA expression of PPAR-γ1 and PPAR-γ2, miR-27a, and miR-27b was measured by qPCR in visceral and subcutaneous adipose tissue. Clinical variables and serum adipokine and hormone levels were correlated with PPAR-γ and miR-27 expression. In addition, a systematic review of the literature regarding PPAR-γ expression in adipose tissue of obese patients was performed. Results We found no differences in the expression of PPAR-γ and miR-27 in adipose tissue of obese patients vs. controls. The literature review revealed discrepant results regarding PPAR-γ expression in adipose tissue of obese patients. Of note, we described a significant negative correlation between pre-operative PPAR-γ1 expression in adipose tissue of obese patients and post-operative weight loss, potentially linked with insulin resistance markers. Conclusion PPAR-γ1 expression in adipose tissue is associated with weight loss after sleeve gastrectomy and may be used as a biomarker for response to surgery.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was funded by the following grants to M.M.: ISCIII and FEDER, PI10/01692, PI16/01548, RD16/0017/0023, and I3SNS-INT12/049, L.H.C.: Junta de Castilla y León GRS 681/A/11, J.-L. T.: GRS 1587/A/17 and GRS1356/A/16, G.S.: ERC 260464, EFSD 2030, MICINNSAF2013-43506-R, and Comunidad de Madrid S2010/BMD-2326. G.S. is an investigator of the Ramón y Cajal Program.Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCL

    PPAR-γ Gene Expression in Human Adipose Tissue Is Associated with Weight Loss After Sleeve Gastrectomy

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    Background: The peroxisome proliferator-activated receptor (PPAR)-γ plays a key role in adipose tissue differentiation and fat metabolism. However, it is unclear which factors may regulate its expression and whether obese patients have changes in adipose tissue expression of PPAR-γor potential regulators such as miR-27. Thus, our aims were to analyze PPAR-γ and miR-27 expression in adipose tissue of obese patients, and to correlate their levels with clinical variables. Subjects and methods: We included 43 morbidly obese subjects who underwent sleeve gastrectomy (31 of them completed 1-year follow-up) and 19 non-obese subjects. mRNA expression of PPAR-γ1 and PPAR-γ2, miR-27a, and miR-27b was measured by qPCR in visceral and subcutaneous adipose tissue. Clinical variables and serum adipokine and hormone levels were correlated with PPAR-γ and miR-27 expression. In addition, a systematic review of the literature regarding PPAR-γ expression in adipose tissue of obese patients was performed. Results: We found no differences in the expression of PPAR-γ and miR-27 in adipose tissue of obese patients vs. controls. The literature review revealed discrepant results regarding PPAR-γ expression in adipose tissue of obese patients. Of note, we described a significant negative correlation between pre-operative PPAR-γ1 expression in adipose tissue of obese patients and post-operative weight loss, potentially linked with insulin resistance markers. Conclusion: PPAR-γ1 expression in adipose tissue is associated with weight loss after sleeve gastrectomy and may be used as a biomarker for response to surgeryThis work was funded by the following grants to M.M.: ISCIII and FEDER, PI10/01692, PI16/01548, RD16/0017/0023, and I3SNS-INT12/049, L.H.C.: Junta de Castilla y León GRS 681/A/11, J.-L. T.: GRS 1587/A/17 and GRS1356/A/16, G.S.: ERC 260464, EFSD 2030, MICINNSAF2013-43506-R, and Comunidad de Madrid S2010/BMD-2326. G.S. is an investigator of the Ramón y Cajal Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ISCIII,PI10/01692,Miguel Marcos,PI16/01548,Miguel Marcos,Gerencia regional de salud,junta de castilla y león,GRS 681/A/11,Lourdes Hernández-Cosido,J.-L. T,Lourdes Hernández-Cosido,Gerencia Regional de Salud,Junta de Castilla y León,GRS 1587/A/17,Jorge-Luis Torres,GRS1356/A/16,Jorge-Luis Torre

    Stable neutralizing antibody levels 6 months after mild and severe COVID-19 episodes

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    Background. Understanding mid-term kinetics of immunity to SARS-CoV-2 is the cornerstone for public health control of the pandemic and vaccine development. However, current evidence is rather based on limited measurements, losing sight of the temporal pattern of these changes. Methods. We conducted a longitudinal analysis on a prospective cohort of COVID-19 patients followed up for >6 months. Neutralizing activity was evaluated using HIV reporter pseudoviruses expressing SARS-CoV-2 S protein. IgG antibody titer was evaluated by ELISA against the S2 subunit, the receptor binding domain (RBD), and the nucleoprotein (NP). Statistical analyses were carried out using mixed-effects models. Findings. We found that individuals with mild or asymptomatic infection experienced an insignificant decay in neutralizing activity, which persisted 6 months after symptom onset or diagnosis. Hospitalized individuals showed higher neutralizing titers, which decreased following a 2-phase pattern, with an initial rapid decline that significantly slowed after day 80. Despite this initial decay, neutralizing activity at 6 months remained higher among hospitalized individuals compared to mild symptomatic. The slow decline in neutralizing activity at mid-term contrasted with the steep slope of anti-RBD, S2, or NP antibody titers, all of them showing a constant decline over the follow-up period. Conclusions. Our results reinforce the hypothesis that the quality of the neutralizing immune response against SARS-CoV-2 evolves over the post-convalescent stage.info:eu-repo/semantics/acceptedVersio
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