Proteome-metabolite interactions in wine. Tracking down the X factor

Mestrado em Viticultura e Enologia - Instituto Superior de AgronomiaProtein haze formation is a recurrent problem in white and rosé wines. Formation of his type of haze is dependent on only for the protein content of the wine, but is strictly related to one or more non-proteinaceous wine components termed as X factor. A selected wine from the variety Moscatel of Alexandria was fractionated using a preparative RP-18 chromatography column in eight methanolic fractions that were subsequently subjected to heat stability test with wine isolated protein in model wine solution. The results obtained indicate that the X factor is not a single compound, but rather an interaction of several compounds with wine proteins. A heat stability test involving the combination of four of these fractions with an isomer of the major compound identified in the water soluble fraction, the erythritol, presented the higher haze formation. Nuclear magnetic resonance (NMR) and mass spectrometer (GCT) analysis allowed the identification of two compounds present in the methanolic fractions. As a whole, this work constitutes a step forward in the difficult process of purification and identification of the wine non-proteinaceous components that modulate the wine protein haze formation

Assessment of Potential Effects of Common Fining Agents Used for White Wine Protein Stabilization

Technical BriefA variety of fining agents are commercially available to the wine industry, including proteins and inorganic ion exchangers. These fining agents are essentially used to control the levels of phenolics in wine, but they also have the potential to interact with other wine components, most often as a side effect. They are therefore expected to influence, at least in part, the potential for wine protein haze formation. Six common fining agents—casein, egg albumin, isinglass, chitosan, chitin, and polyvinylpolypyrrolidone (PVPP)—were analyzed to assess their effects on wine protein haze-forming potential and on the levels of proteins and phenolic compounds in a Muscat of Alexandria wine. Bentonite was selected as the positive control, whereas nonfined wine was used as the negative control. Differential results were detected among the selected fining agents when compared to the controls. Egg albumin and chitosan, although incapable of stabilizing the wine, originated a small but significant decrease in the protein haze formed, whereas chitosan and PVPP were second to bentonite in removing the most polyphenols from the wine. Thus, while chitosan fining removes a fraction of polyphenols from the wine and seems to induce a small decrease in its haze-forming potential, PVPP eliminates more polyphenols while leaving its haze-forming potential unaltered. The fining agents analyzed did not significantly affect wine protein content but did remove considerable levels of polyphenols and presented no apparent effect on protein stabilization of the fined wines. Results show that these fining agents do not contribute significantly to protein stabilization in white wines, confirming that bentonite was the most effective agent in wine protein stabilizationinfo:eu-repo/semantics/publishedVersio

The effect of dicarboxymethyl cellulose on the prevention of protein haze formation on white wine

Funding Information: This research was funded by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) and by Funda??o para a Ci?ncia e a Tecnologia (FCT) under the PhD grant DFA/BD/5529/2020. FCT/MCTES is also acknowledged for the National NMR Facility (RECI/BBB-BQB/0230/2012 and RECI/BBB-BEP/0124/2012). We acknowledge the NMR laboratory and the analysis laboratory at LAQV REQUIMTE, Chemistry Department, NOVA School of Science and Technology, Portugal for the NMR, ICP-AES and XRD data obtained. We thank Enartis for sharing their knowledge, insight, and experience. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Wine clarity is a critical aspect in the commercialization of white wines. The formation of wine haze can be attributed to the aggregation and precipitation of heat-unstable wine proteins. Bentonite fining is the commonly used method in winemaking for protein removal, but it is responsible for loss of wine volume and quality. Dicarboxymethyl cellulose (DCMC) was developed as a potential alternative to bentonite. Water-insoluble DCMC was prepared via catalyzed heterogeneous etherification using sodium chloromalonate and potassium iodide. White wine fining trials were benchmarked with different dosages of DCMC against a bentonite. A high-performance liquid chromatography method was optimized for protein quantification. The samples underwent heat stability tests to evaluate wine turbidity before and after fining. Results show that DCMC successfully reduced the wine protein content and turbidity. DCMC produced heat-stable wines with dosages higher than 0.25 g/L. The innovative application of DCMC in the wine sector shows potential due to its ability to stabilize white wines while overcoming problems associated with bentonite, such as lees production and loss of wine, contributing to a more sustainable process.publishersversionpublishe

Synthesis and characterization of dicarboxymethyl cellulose

Associate Laboratory for Green Chemistry-LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). FCT, Portugal (ROTEIRO/0031/2013–PINFRA/22161/2016, co-financed by FEDER through COMPETE 2020, POCI, and PORL and FCT through PIDDAC).Synthesis of dicarboxymethyl cellulose (DCMC) under heterogeneous conditions was examined. Cellulose was etherified using sodium bromomalonate in isopropanol/water in the presence of NaOH. The reaction was performed with five different NaOH concentrations (5–30 w/v %) and the products were characterized by anion-exchange high-performance liquid chromatography (AE-HPLC), inductively coupled plasma atomic emission- (ICP-AES), Fourier transform infrared- (FTIR), and nuclear magnetic resonance (1D-/2D-NMR) spectroscopy. Adjusting the amount of NaOH resulted in increasing functionalization of the cellulose achieving an average degree of substitution (DS) between 0.05 and 0.51. Both ICP-AES and AE-HPLC gave comparable DS values. NMR spectroscopic analysis showed that etherification occurred preferably at O-6 and, to a certain extent, at the secondary positions depending on the reaction conditions. Graphic abstract: [Figure not available: see fulltext.].authorsversionpublishe

A transferência de conhecimento para a melhoria do trabalho numa academia de formação

Neste tempo de mudança célere, quase imediata, onde os resultados reclamados pelas organizações são acompanhados de exigência contígua aos colaboradores, a capacidade de aprender mais rápido do que os adversários pode ser uma das vantagens competitivas mais sustentáveis para uma organização. A formação é entendida como o pilar essencial para a interligação entre as políticas de Gestão de Pessoas, as estratégias e os objetivos de negócio de qualquer empresa, podendo contribuir para uma experiência única dos colaboradores com impactos reais nos resultados organizacionais. Mediante a análise realizada ao contexto da empresa e às políticas de Gestão de Pessoas, resulta a proposta de uma academia de formação e a revisão dos processos de avaliação formativo, nomeadamente, a transferência do conhecimento. Para a estruturação da Academia de Formação deste projeto, foi realizado um benchmark sobre duas academias empresariais em Portugal, através de entrevistas realizadas a dois Diretores de Recursos Humanos. Relativamente ao processo de transferência de conhecimento, foram realizadas 8 entrevistas a formadores com o objetivo de perceber os desafios, as estratégias e as práticas utilizadas para transferir conhecimento para o posto de trabalho. Adicionalmente, foi efetuado um teste com um grupo piloto por forma a identificar os níveis de transferência de conhecimento numa determinada formação na empresa em estudo.In a time of fast, almost immediate change, where the results claimed by organizations are accompanied by contiguous demands on employees, the ability to learn faster than opponents can be one of the most sustainable competitive advantages for an organization. Training is understood as the essential pillar for the interconnection between People Management policies, strategies and business objectives of any company, and can contribute to a unique experience of employees with real impacts on organizational results. Through the analysis carried out in the context of the company and the people management policies, results the proposal of a training academy and the revision of the formative evaluation processes, namely, the knowledge transfer. For the structuring of the Training Academy of this project, a reference was made in two printed academies in Portugal, through interviews with two Human Resources Directors. Regarding the process of knowledge transfer, 8 interviews were conducted with trainers in order to understand the challenges, strategies and practices used to transfer knowledge to the workplace. Additionally, a pilot group test was conducted to identify the levels of knowledge transfer in a given training in the company under study

Ocorrência do besouro tigre Neotropical, Cylindera suturalis helvaea (W. Horn, 1903) (Coleoptera, Cicindelidae), em bancos de areia do Reservatório da Barragem da Pedra, Rio de Contas, Bahia, Brasil

The occurrence of the tiger beetle Cylindera (Plectographa) suturalis helvaea (W. Horn, 1903) in sandbanks of Reservoir Barragem da Pedra, Bahia, Brazil, is registered. Adult beetles were collected using entomological nets on sandbanks between May and September 2003. Cicindelids are one of the most conspicuous groups among invertebrates. That is why thay are well suited for bioindication of biodiversity as well as of disturbance and modification in terrestrial ecosystems.Neste estudo é registrada a ocorrência do besouro tigre Cylindera (Plectographa) suturalis helvaea (W. Horn, 1903) em bancos de areia no Reservatório da Barragem da Pedra, Bahia, Brasil. Indivíduos adultos foram coletados utilizando-se de redes entomológicas, entre os meses de Maio e Setembro de 2003. Cicindelídeos são um dos mais conspícuos grupos de invertebrados, sendo considerados como bons indicadores de biodiversidade assim como de distúrbios e modificações nos ecossistemas terrestres

Rede de bibliotecas em ambientes de saúde mental

Dissertação (mestrado profissional) - Universidade do Estado de Santa Catarina, Centro de Ciências Humanas e da Educação, Programa de Pós-Graduação em Gestão da Informação, Florianópolis, 2017.O objetivo geral desta pesquisa consistiu em apresentar uma proposta de implantação de uma rede de bibliotecas nos Centros de Atenção Psicossocial (CAPS) de Florianópolis (SC), pautada no potencial social e cultural das atividades da instituição biblioteca, para a promoção à saúde mental de pessoas em sofrimento psíquico. Na fundamentação conceitual abordou-se a história da loucura e o deslocamento da atenção em saúde mental dos modelos manicomiais para as novas abordagens dos serviços em saúde mental baseada na comunidade. Enfatizou-se a importância da interdisciplinaridade como possibilidade da criação do diálogo entre os campos da Biblioteconomia e da Saúde Mental. Discutiu-se a relação entre bibliotecas, sociedade e a atuação ético-política do bibliotecário. Tratou-se do conceito de redes e dos serviços de biblioteca, tais como: formação e desenvolvimento da coleção, tratamento técnico da coleção, disseminação e mediação da informação, a ação cultural e a biblioterapia. Na fundamentação teórico-metodológica abordou-se a fenomenologia do mundo da vida de Edmund Husserl e a fenomenologia social de Alfred Schütz. Tratou-se, também, do construtivismo social de Norbert Elias, Berger e Luckmann, além da teoria das representações sociais de Serge Moscovici. A pesquisa é do tipo qualitativa e foram entrevistados profissionais e usuários dos CAPS, mediante roteiro de entrevista e questionário de caracterização. Para a análise dos discursos utilizou-se a técnica do Discurso do Sujeito Coletivo (DSC). A partir dos discursos coletados nas entrevistas e das representações sociais resumidas nos DSCs, constatou-se a necessidade da criação de uma rede de bibliotecas nos Centros de Atenção Psicossocial (CAPS), com o intuito de potencializar a rede de Saúde Mental do Município de Florianópolis (SC). Apresentou-se, por fim, uma proposta teórico-metodológica para a implantação da Rede de bibliotecas CAPS com a atuação e presença de um profissional bibliotecário.Abstract : The general objective of this research was to present a proposal for the implementation of a network of libraries in the Centers for Psychosocial Attention (CAPS) in Florianópolis (SC), based on the social and cultural potential of the activities of the library institution in promoting the mental health of people in psychological distress. In the conceptual basis, the history of madness and the displacement of the attention in mental health of the manicomial models for the new approaches of the services in mental health based on the community were approached. It was emphasized the importance of interdisciplinarity as a possibility for the creation of dialogue between the fields of Librarianship and Mental Health. The relationship between libraries, society and the librarian's ethical-political action was discussed. It was the concept of library networks and services, such as: collection training and development, collection technical treatment, information dissemination and mediation, cultural action and bibliotherapy. In the theoretical-methodological foundations the phenomenology of the world of the life of Edmund Husserl and the social phenomenology of Alfred Schütz was approached. It was also the social constructivism of Norbert Elias, Berger and Luckmann, as well as Serge Moscovici's theory of social representations. The research is of the qualitative type and interviewed professionals and users of the CAPS, through interview script and characterization questionnaire. For the analysis of the speeches the technique of the Collective Subject Discourse (DSC) was used. From the discourses collected in interviews and social representations summarized in the DSCs, it was verified the need to create a network of libraries in the Center for Psychosocial Attention (CAPS), with the aim of strengthening the Mental Health Network of the Municipality of Florianópolis (SC). Finally, a theoretical-methodological proposal for the implementation of the "Network of CAPS libraries" was presented, with the presence and presence of a professional librarian

Do nada de ser ao ser possível: a construção de si como movimento da consciência em Sartre

TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Filosofia e Ciências Humanas. Filosofia.Esta pesquisa tem como objetivo compreender o paradoxo da consciência enquanto movimento na construção de si na filosofia de Jean-Paul Sartre, uma vez que essa construção implica um nada de ser e ao mesmo tempo aponta para um ser possível, para um projeto fundamental. Mas afinal, como que a consciência que é fundamentada como um nada de ser pode possibilitar um ser possível? Em outras palavras, como algo que é um nada de ser pode dar origem a um ser possível? Para resolver essa implicação paradoxal foi preciso partir da importante distinção que Sartre realizou em sua teoria entre consciência e psiquismo. Para o filósofo francês, jamais a consciência poderá ser entendida como uma substância ou como um lugar que habita o Eu, mas ela deve ser compreendida como uma estrutura vazia e intencional voltada para o mundo em busca de realização. Sendo a consciência uma estrutura vazia, possibilitará a construção de si como deslize entre o nada de ser e o ser possível, ou seja, é quando o homem diante do nada e, projetado para o futuro, construirá o seu ter-de-ser como valor, isto é, a sua essência. Conclui-se que a consciência apresenta um papel primordial para a construção de si, mesmo diante desse paradoxo apresentado acima e que será discutido neste estudo.This research aims to understand the paradox of consciousness as a movement in the construction of self in the philosophy of Jean-Paul Sartre, since this construction implies a nothingness and at the same time points to a possible being, to a fundamental project. But after all, how can consciousness that is grounded as a nothingness enable a possible being? In other words, how can something that is a nothingness give rise to a possible being? To solve this paradoxical implication it was necessary to start from the important distinction Sartre made in his theory between consciousness and psyche. For the French philosopher, consciousness can never be understood as a substance or as a place that inhabits the Self, but it must be understood as an empty and intentional structure turned to the world for fulfillment. As consciousness is an empty structure, it will enable the construction of itself as a slip between nothingness and being possible, that is, when man facing nothingness and, projected into the future, will build your having to be as value, that is, its essence. It is concluded that consciousness has a primordial role for the construction of itself, even in face of this paradox presented above and that will be discussed in this study