Early acute microvascular kidney transplant rejection in the absence of anti-HLA antibodies is associated with preformed IgG antibodies against diverse glomerular endothelial cell antigens

International audienceBACKGROUND: Although anti-HLA antibodies (Abs) cause most antibody-mediated rejections of renal allografts, non-anti-HLA Abs have also been postulated to contribute. A better understanding of such Abs in rejection is needed.METHODS: We conducted a nationwide study to identify kidney transplant recipients without anti-HLA donor-specific Abs who experienced acute graft dysfunction within 3 months after transplantation and showed evidence of microvascular injury, called acute microvascular rejection (AMVR). We developed a crossmatch assay to assess serum reactivity to human microvascular endothelial cells, and used a combination of transcriptomic and proteomic approaches to identify non-HLA Abs.RESULTS: We identified a highly selected cohort of 38 patients with early acute AMVR. Biopsy specimens revealed intense microvascular inflammation and the presence of vasculitis (in 60.5%), interstitial hemorrhages (31.6%), or thrombotic microangiopathy (15.8%). Serum samples collected at the time of transplant showed that previously proposed anti-endothelial cell Abs-angiotensin type 1 receptor (AT1R), endothelin-1 type A and natural polyreactive Abs-did not increase significantly among patients with AMVR compared with a control group of stable kidney transplant recipients. However, 26% of the tested AMVR samples were positive for AT1R Abs when a threshold of 10 IU/ml was used. The crossmatch assay identified a common IgG response that was specifically directed against constitutively expressed antigens of microvascular glomerular cells in patients with AMVR. Transcriptomic and proteomic analyses identified new targets of non-HLA Abs, with little redundancy among individuals.CONCLUSIONS: Our findings indicate that preformed IgG Abs targeting non-HLA antigens expressed on glomerular endothelial cells are associated with early AMVR, and that cell-based assays are needed to improve risk assessments before transplant

Subarachnoid anesthesia in sedated calves undergoing umbilical surgery: Comparison of procaine 2% and lidocaine 2% associated with xylazine 2%

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Nouvelle lecture des phases de la dernière déglaciation dans le Loch Sunart (Nord-Ouest Ecosse) basée sur des traceurs géochimiques

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Nouvelle lecture des phases de la dernière déglaciation dans le Loch Sunart (Nord-Ouest Ecosse) basée sur des traceurs géochimiques

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Nouvelle lecture des phases de la dernière déglaciation dans le Loch Sunart (Nord-Ouest Ecosse) basée sur des traceurs géochimiques

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Aminoaciduria caused by fanconi syndrome in a heifer

A case study of renal tubular dysfunction consistent with idiopathic Fanconi syndrome is reported in an 18-month-old Holstein heifer. The clinical, biochemical, and histopathological features are described. The heifer had clinical signs of growth retardation, wasting, and persistent diarrhea. Biochemical blood analysis identified hypokalemia, hyponatremia, and hypo-chloremia. Urinalysis identified glycosuria, proteinuria, and acidic pH. Histological examination of the kidney disclosed mild tubular necrosis with proteinaceous casts in the lumina of renal tubules. We performed LC-HRMS on urine to confirm Fanconi syndrome. Using this technique, we identified severe generalized aminoaciduria suggestive of idiopathic renal Fanconi syndrome in this heifer

Pan-European Satellite-Derived Coastal Bathymetry-Review, User Needs and Future Services

Low-lying coastal zones are home to around 10% of the world’s population and to many megacities. Coastal zones are largely vulnerable to the dynamics of natural and human-induced changes. Accurate large-scale measurements of key parameters, such as bathymetry, are needed to understand and predict coastal changes. However, nearly 50% of the world’s coastal waters remain unsurveyed and for a large number of coastal areas of interest, bathymetric information is unavailable or is often decades old. This lack of information is due to the high costs in time, money and safety involved in collecting these data using conventional echo sounder on ships or LiDAR on aircrafts. Europe is no exception, as European seas are not adequately surveyed according to the International Hydrographic Organisation. Bathymetry influences ocean waves and currents, thereby shaping sediment transport which may alter coastal morphology over time. This paper discusses state-of-the-art coastal bathymetry retrieval methods and data, user requirements and key drivers for many maritime sectors in Europe, including advances in Satellite-Derived Bathymetry (SDB). By leveraging satellite constellations, cloud services and by combining complementary methods, SDB appears as an effective emerging tool with the best compromise in time, coverage and investment to map coastal bathymetry and its temporal evolution

EasyMatch-R: a web application to facilitate donor query in Hematopoietic Stem Cell Transplantation

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Easy-HLA : logiciel en ligne d’haplotypage HLA accessible à tous délivrant un panel complet destiné aux analyses génétiques

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SNP-HLA Reference Consortium : HLA and SNP data sharing for promoting HLA centric analyses in genomics

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