Gut microbiome specific changes in different behavioral profiles in a mouse social defeat stress model.

Comunicación de tipo PósterThe gut microbiome has arisen as one important modulator of general health, including brain function. In fact, disturbances in brain health are commonly mirrored in the microbiome, which could be contributing to pathology. One of the most common brain disorders is depression, which is tightly linked to environmental factors such as stress and drives alterations in regular behavior. However, not much is known about the role of the gut microbiome in response to stress and its relationship to behavior. In this study, the social defeat stress (SDS) paradigm was used as a depressive-like symptoms inducer in 8 w.o. male C57BL/6J mice for 10 days. Mice were segregated in stress resilient and sensitive according to behavior using K-means clustering and behavioral data was interpreted using principal component analysis. Then, the mice microbiome was extracted from fecal pellets after the stress protocol. DNA was extracted and purified followed by 16S (V3-V4) region amplification for sequencing. These data were analyzed to obtain diversity indexes and identify bacterial taxa within samples and groups. Data revealed that mice responded differently to the same stressor. Half the mice were found to have mild depressive-like symptoms whereas the other half showed profound alterations. Behavioral data was found to be explained in three factors: anhedonia, exploration, and motility. Stressed mice showed overall differences in their microbiome, being less diverse and populations associated with higher inflammation. Moreover, the healthy gut associated Verrucomicrobiae class was only identified in stress resilient mice, suggesting a possible relationship with their behavioral phenotype. Altogether, these results show a different behavioral response to stress in animals that reflects in their microbiome, which could be a key factor in determining stress resilience.This study was supported by Ministerio de Ciencia e Innovación - Plan Nacional I+D+I from Spain (PID2020-117464RB-I00) to CP and MP-M; FEDER/Junta de Andalucía - Proyectos I+D+I en el marco del Programa Operativo FEDER Andalucía 2014-2020 (UMA20-FEDERJA-112) to CP and MP-M; Consejería de Conocimiento, Investigación y Universidades, Junta de Andalucía (P20_00460) to CP. PC-P has been funded by the research project PID2020-117464RB-I00. Ministerio de Educación, Cultura y Deporte from Spain (FPU19/03629 to Infantes-López MI). Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

The original version of this article unfortunately contained a mistake

Comparación de regiones hipervariables del gen rRNA 16S para el estudio de la microbiota intestinal en acuicultura

Skin lesions are a frequent fact associated with intensive conditions affecting farmed fish. Knowing that the use of probiotics can improve fish skin health, SpPdp11 dietary administration has demonstrated beneficial effects for farmed fish, so its potential on the skin needs to be studied more deeply. The wounded specimens that received the diet with SpPdp11 showed a decrease in the abundance of Enterobacteriaceae, Photobacterium and Achromobacter related to bacterial biofilm formation, as well as the overexpression of genes involved in signaling mechanisms (itpr3), cell migration and differentiation (panxa, ttbk1a, smpd3, vamp5); and repression of genes related to cell proliferation (vstm4a, areg), consistent with a more efficient skin healing processes than that observed in the wounded control group. In addition, among the groups of damaged skin with different diets, Achromobacter, f_Ruminococcaceae, p_Bacteroidetes, Fluviicola and Flavobacterium genera with significant differences showed positive correlations with genes related to cell migration and negative correlations with inflammation and cell proliferation and may be the target of future studies.Este estudio fue apoyado por subvenciones del Ministerio de Economía y Competitividad (MINECO) (AGL-2017-83370-C1-R y AGL-2017-83370-C3-R). Partial funding for open access charge: Universidad de Málag

Comparación de regiones hipervariables del gen rRNA 16S para el estudio de la microbiota intestinal en acuicultura

El mantenimiento de la biodiversidad en el intestino de los peces es esencial para su estado de salud y su defensa frente a posibles patógenos. Una manera habitual de estudiar esta biodiversidad es mediante la técnica de metabarcoding, que consiste en la secuenciación de regiones hipervariables del gen rRNA 16S. En este trabajo, se han comparado las regiones hipervariables V1V3, V3V4 y V4V5 mediante un flujo bioinformático para determinar cuál es la más apropiada para el estudio de la microbiota de dos especies de interés en acuicultura: la dorada y el lenguado. Tanto los resultados de diversidad alfa como de taxonomía obtenidos apuntan a que la región idónea para ello en estos modelos es la V3V4. Por tanto, es la región que debería emplearse para conocer la biodiversidad mediante metabarcoding en el intestino de peces cultivables, algo de gran relevancia en muchos estudios en acuicultura, puesto que es un indicador de la salud de los individuos.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Respuesta transcriptómica del intestino de Solea senegalensis tras la administración dietética del probiótico Shewanella putrefaciens Pdp11

El uso de probióticos en acuicultura mejora la salud y el bienestar de los animales, en el caso del probiótico SpPdp11 se han observado numerosos beneficios a nivel de inmunidad, estrés y microbiota intestinal. Sin embargo, no se ha realizado un análisis transcriptomico a nivel intestinal del efecto de este microorganismo en la dieta. Se realizó una extracción de ARN de una sección anterior y posterior del intestino Solea senegalensis y un siguiente análisis de RNA-seq. Los resultados mostraron en general una disminución de genes relacionados con la división celular en intestino anterior frente al aumento en el metabolismo de lípidos de la parte posterior. La inclusión de sPpd11 en la dieta también mostró una disminución de la oxidación en ácidos grasos, estrés oxidativo o respuesta inflamatoria en el intestino anterior de los peces de estudio.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech