Cerebellar–M1 connectivity changes associated with motor learning are somatotopic specific

One of the functions of the cerebellum in motor learning is to predict and account for systematic changes to the body or environment. This form of adaptive learning is mediated by plastic changes occurring within the cerebellar cortex. The strength of cerebellar-to-cerebral pathways for a given muscle may reflect aspects of cerebellum-dependent motor adaptation. These connections with motor cortex (M1) can be estimated as cerebellar inhibition (CBI): a conditioning pulse of transcranial magnetic stimulation delivered to the cerebellum before a test pulse over motor cortex. Previously, we have demonstrated that changes in CBI for a given muscle representation correlate with learning a motor adaptation task with the involved limb. However, the specificity of these effects is unknown. Here, we investigated whether CBI changes in humans are somatotopy specific and how they relate to motor adaptation. We found that learning a visuomotor rotation task with the right hand changed CBI, not only for the involved first dorsal interosseous of the right hand, but also for an uninvolved right leg muscle, the tibialis anterior, likely related to inter-effector transfer of learning. In two follow-up experiments, we investigated whether the preparation of a simple hand or leg movement would produce a somatotopy-specific modulation of CBI. We found that CBI changes only for the effector involved in the movement. These results indicate that learning-related changes in cerebellar– M1 connectivity reflect a somatotopy-specific interaction. Modulation of this pathway is also present in the context of interlimb transfer of learning

A FLAMINGOS Deep Near Infrared Imaging Survey of the Rosette Complex I: Identification and Distribution of the Embedded Population

We present the results of a deep near-infrared imaging survey of the Rosette Complex. We studied the distribution of young embedded sources using a variation of the Nearest Neighbor Method applied to a carefully selected sample of near-infrared excess (NIRX) stars which trace the latest episode of star formation in the complex. Our analysis confirmed the existence of seven clusters previously detected in the molecular cloud, and identified four more clusters across the complex. We determined that 60% of the young stars in the complex and 86% of the stars within the molecular cloud are contained in clusters, implying that the majority of stars in the Rosette formed in embedded clusters. We compare the sizes, infrared excess fractions and average extinction towards individual clusters to investigate their early evolution and expansion. We found that the average infrared excess fraction of clusters increases as a function of distance from NGC 2244, implying a temporal sequence of star formation across the complex. This sequence appears to be primordial, possibly resulting from the formation and evolution of the molecular cloud and not from the interaction with the HII region.Comment: Accepted by Astrophysical Journa

Laterality Differences in Cerebellar-Motor Cortex Connectivity

Lateralization of function is an important organizational feature of the motor system. Each effector is predominantly controlled by the contralateral cerebral cortex and the ipsilateral cerebellum. Transcranial magnetic stimulation studies have revealed hemispheric differences in the stimulation strength required to evoke a muscle response from the primary motor cortex (M1), with the dominant hemisphere typically requiring less stimulation than the nondominant. The current study assessed whether the strength of the connection between the cerebellum and M1 (CB-M1), known to change in association with motor learning, have hemispheric differences and whether these differences have any behavioral correlate. We observed, in right-handed individuals, that the connection between the right cerebellum and left M1 is typically stronger than the contralateral network. Behaviorally, we detected no lateralized learning processes, though we did find a significant effect on the amplitude of reaching movements across hands. Furthermore, we observed that the strength of the CB-M1 connection is correlated with the amplitude variability of reaching movements, a measure of movement precision, where stronger connectivity was associated with better precision. These findings indicate that lateralization in the motor system is present beyond the primary motor cortex, and points to an association between cerebellar M1 connectivity and movement execution

Consensus Paper: Neurophysiological Assessments of Ataxias in Daily Practice

The purpose of this consensus paper is to review electrophysiological abnormalities and to provide a guideline of neurophysiological assessments in cerebellar ataxias. All authors agree that standard electrophysiological methods should be systematically applied in all cases of ataxia to reveal accompanying peripheral neuropathy, the involvement of the dorsal columns, pyramidal tracts and the brainstem. Electroencephalography should also be considered, although findings are frequently non-specific. Electrophysiology helps define the neuronal systems affected by the disease in an individual patient and to understand the phenotypes of the different types of ataxia on a more general level. As yet, there is no established electrophysiological measure which is sensitive and specific of cerebellar dysfunction in ataxias. The authors agree that cerebellar brain inhibition (CBI), which is based on a paired-pulse transcranial magnetic stimulation (TMS) paradigm assessing cerebellar-cortical connectivity, is likely a useful measure of cerebellar function. Although its role in the investigation and diagnoses of different types of ataxias is unclear, it will be of interest to study its utility in this type of conditions. The authors agree that detailed clinical examination reveals core features of ataxia (i.e., dysarthria, truncal, gait and limb ataxia, oculomotor dysfunction) and is sufficient for formulating a differential diagnosis. Clinical assessment of oculomotor function, especially saccades and the vestibulo-ocular reflex (VOR) which are most easily examined both at the bedside and with quantitative testing techniques, is of particular help for differential diagnosis in many cases. Pure clinical measures, however, are not sensitive enough to reveal minute fluctuations or early treatment response as most relevant for pre-clinical stages of disease which might be amenable to study in future intervention trials. The authors agree that quantitative measures of ataxia are desirable as biomarkers. Methods are discussed that allow quantification of ataxia in laboratory as well as in clinical and real-life settings, for instance at the patients' home. Future studies are needed to demonstrate their usefulness as biomarkers in pharmaceutical or rehabilitation trials

Turbulent Gas Flows in the Rosette and G216-2.5 Molecular Clouds: Assessing Turbulent Fragmentation Descriptions of Star Formation

The role of turbulent fragmentation in regulating the efficiency of star formation in interstellar clouds is examined from new wide field imaging of 12CO and 13CO J=1-0 emission from the Rosette and G216-2.5 molecular clouds. The Rosette molecular cloud is a typical star forming giant molecular cloud and G215-2.5 is a massive molecular cloud with no OB stars and very little low mass star formation. The properties of the turbulent gas flow are derived from the set of eigenvectors and eigenimages generated by Principal Component Analysis of the spectroscopic data cubes. While the two clouds represent quite divergent states of star formation activity, the velocity structure functions for both clouds are similar. The sonic scale, lambda_S, defined as the spatial scale at which turbulent velocity fluctuations are equivalent to the local sound speed, and the turbulent Mach number evaluated at 1 pc, M_{1pc}, are derived for an ensemble of clouds including the Rosette and, G216-2.5 regions that span a large range in star formation activity. We find no evidence for the positive correlations between these quantities and the star formation efficiency, that are predicted by turbulent fragmentation models. A correlation does exist between the star formation efficiency and the sonic scale for a subset of clouds with L_{FIR}/M(H_2) > 1 that are generating young stellar clusters. Turbulent fragmentation must play a limited and non-exclusive role in determining the yield of stellar masses within interstellar clouds.Comment: Accepted by ApJ, 22 pages, 7 figure

Ten Million Degree Gas in M 17 and the Rosette Nebula: X-ray Flows in Galactic H II Regions

We present the first high-spatial-resolution X-ray images of two high-mass star forming regions, the Omega Nebula (M 17) and the Rosette Nebula (NGC 2237--2246), obtained with the Chandra X-ray Observatory Advanced CCD Imaging Spectrometer (ACIS) instrument. The massive clusters powering these H II regions are resolved at the arcsecond level into >900 (M 17) and >300 (Rosette) stellar sources similar to those seen in closer young stellar clusters. However, we also detect soft diffuse X-ray emission on parsec scales that is spatially and spectrally distinct from the point source population. The diffuse emission has luminosity L_x ~ 3.4e33 ergs/s in M~17 with plasma energy components at kT ~0.13 and ~0.6 keV (1.5 and 7 MK), while in Rosette it has L_x \~6e32 ergs/s with plasma energy components at kT ~0.06 and ~0.8 keV (0.7 and 9 MK). This extended emission most likely arises from the fast O-star winds thermalized either by wind-wind collisions or by a termination shock against the surrounding media. We establish that only a small portion of the wind energy and mass appears in the observed diffuse X-ray plasma; in these blister H II regions, we suspect that most of it flows without cooling into the low-density interstellar medium. These data provide compelling observational evidence that strong wind shocks are present in H II regions.Comment: 35 pages, including 11 figures; to appear in ApJ, August 20, 2003. A version with high-resolution figures is available at ftp://ftp.astro.psu.edu/pub/townsley/diffuse.ps.g

The dark matter halo shape of edge-on disk galaxies - I. HI observations

This is the first paper of a series in which we will attempt to put constraints on the flattening of dark halos in disk galaxies. We observe for this purpose the HI in edge-on galaxies, where it is in principle possible to measure the force field in the halo vertically and radially from gas layer flaring and rotation curve decomposition respectively. In this paper, we define a sample of 8 HI-rich late-type galaxies suitable for this purpose and present the HI observations.Comment: Accepted for publication by Astronomy & Astrophysics. For a higher resolution version see http://www.astro.rug.nl/~vdkruit/jea3/homepage/12565.pd

A Robotic Wide-Angle H-Alpha Survey of the Southern Sky

We have completed a robotic wide-angle imaging survey of the southern sky (declination less than +15 degrees) at 656.3 nm wavelength, the H-alpha emission line of hydrogen. Each image of the resulting Southern H-Alpha Sky Survey Atlas (SHASSA) covers an area of the sky 13 degrees square at an angular resolution of approximately 0.8 arcminute, and reaches a sensitivity level of 2 rayleigh (1.2 x 10^-17 erg cm^-2 s^-1 arcsec^-2) per pixel, corresponding to an emission measure of 4 cm^-6 pc, and to a brightness temperature for microwave free-free emission of 12 microkelvins at 30 GHz. Smoothing over several pixels allows features as faint as 0.5 rayleigh to be detected.Comment: LATEX, 33 pages, 15 figures. Accepted for publication in PASP, 113, November 2001. Further information at http://amundsen.swarthmore.edu/SHASSA

A Chandra Study of the Rosette Star-Forming Complex. I. The Stellar Population and Structure of the Young Open Cluster NGC 2244

We present the first high spatial resolution Chandra X-ray study of NGC 2244, the 2 Myr old stellar cluster immersed in the Rosette Nebula. Over 900 X-ray sources are detected; 77% have optical or FLAMINGOS near-infrared (NIR) stellar counterparts and are mostly previously uncatalogued young stellar cluster members. All known OB stars with spectral type earlier than B1 are detected and the X-ray selected stellar population is estimated to be nearly complete between 0.5 and 3 Msun. The X-ray luminosity function (XLF) ranges from 29.4<logLx<32 ergs/s in the hard (2-8keV) band. By comparing the NGC 2244 and Orion Nebula Cluster XLFs, we estimate a total population of 2000 stars in NGC 2244. A number of further results emerge from our analysis: The XLF and the associated K-band luminosity function indicate a normal Salpeter initial mass function (IMF) for NGC 2244. This is inconsistent with the top-heavy IMF reported from earlier optical studies that lacked a good census of <4Msun stars. The spatial distribution of X-ray stars is strongly concentrated around the central O5 star, HD 46150. The other early O star, HD 46223, has few companions. The cluster's stellar radial density profile shows two distinctive structures. This double structure, combined with the absence of mass segregation, indicates that this cluster is not in dynamical equilibrium. The spatial distribution of X-ray selected K-excess disk stars and embedded stars is asymmetric with an apparent deficit towards the north. The fraction of X-ray-selected cluster members with K-band excesses caused by inner protoplanetary disks is 6%, slightly lower than the 10% disk fraction estimated from the FLAMINGOS study based on the NIR-selected sample. This is due to the high efficiency of X-ray surveys in locating disk-free T Tauri stars.[Abridged]Comment: Accepted for publication in ApJ (March 1, 2008 v675 issue). 61 pages, 20 figures, 7 tables. Updated a statement on NGC 2244-334 and added a referenc

Occlusion of LTP-Like Plasticity in Human Primary Motor Cortex by Action Observation

Passive observation of motor actions induces cortical activity in the primary motor cortex (M1) of the onlooker, which could potentially contribute to motor learning. While recent studies report modulation of motor performance following action observation, the neurophysiological mechanism supporting these behavioral changes remains to be specifically defined. Here, we assessed whether the observation of a repetitive thumb movement – similarly to active motor practice – would inhibit subsequent long-term potentiation-like (LTP) plasticity induced by paired-associative stimulation (PAS). Before undergoing PAS, participants were asked to either 1) perform abductions of the right thumb as fast as possible; 2) passively observe someone else perform thumb abductions; or 3) passively observe a moving dot mimicking thumb movements. Motor evoked potentials (MEP) were used to assess cortical excitability before and after motor practice (or observation) and at two time points following PAS. Results show that, similarly to participants in the motor practice group, individuals observing repeated motor actions showed marked inhibition of PAS-induced LTP, while the “moving dot” group displayed the expected increase in MEP amplitude, despite differences in baseline excitability. Interestingly, LTP occlusion in the action-observation group was present even if no increase in cortical excitability or movement speed was observed following observation. These results suggest that mere observation of repeated hand actions is sufficient to induce LTP, despite the absence of motor learning