FICCIONES DE LO REAL. UN ACERCAMIENTO A LA VIOLENCIA MEDIANTE LA PINTURA Y LA FOTOGRAFIA

[EN] Fictions of the real aims to be an artistic project that approaches a plastic mode of representation of violence from a debtor iconography of cinema, literature and music, and allowing, in turn, reflect on the human ambiguity from pictorial and photographic image. The beauty of the grotesque and the human turbid. The picture as a window into a world where prevailing poetic anguish, where our senses are dimmed and where the doubts and questions arise. Fictionalized realities and fictions that show more than reality. That which is hidden, paradoxically, is closest to the truth. Following the words of John Berger, "only what he is capable of narrating can make us understand", I understand that this narrative that allows the image through symbols, metaphors and other figures of speech is leading the viewer to a new reality fraught mysteries, new questions by suggestion.[ES] Ficciones de lo real pretende ser un proyecto artístico que se acerque de un modo plástico a la representación de la violencia desde una iconografía deudora del cine, la literatura y la música, y que permita, a su vez, reflexionar sobre la ambigüedad humana desde de la imagen pictórica y fotográfica. La belleza de lo grotesco y lo turbio del ser humano. El cuadro como ventana a un mundo donde imperan poéticas de angustia, donde nuestros sentidos se eclipsan y donde surgen las dudas y los interrogantes. Realidades ficcionadas y ficciones que nos muestran más que la propia realidad. Aquello que se encuentra oculto, es paradójicamente, lo más cercano a la verdad. Siguiendo las palabras de John Berger, “sólo lo que es capaz de narrar puede hacernos comprender”1, entiendo que esta narración que permite la imagen mediante símbolos, metáforas y demás figuras retóricas es la que conduce al espectador a una nueva realidad plagada de misterios, sugestionándole nuevos interrogantes.Ciscar Cebria, A. (2015). FICCIONES DE LO REAL. UN ACERCAMIENTO A LA VIOLENCIA MEDIANTE LA PINTURA Y LA FOTOGRAFIA. http://hdl.handle.net/10251/57261.TFG

The act of seeing with no eyes: a videographic project about visuality as an instrument of power

[EN] This article focuses on the video pieces from the project The act of seeing with no eyes (2020). The project consists of two videos that explore the concept of visuality as an instrument of power and a legitimising tool for the politics of domination and inequality. By the use of appropriated archive images, and using collage as the main montage technique, as well as a deconstructive strategy of appropriated material - the images are cut, superimposed and juxtaposed in a plastic way -, relations and contrasts are established between images captured by control and surveillance devices, together with different tests of visual perception. Through the formal and thematic analysis of the video pieces proposed in the project, as well as the study and comparison with other artistic references, the intention is to rethink the dialectic of the montage used, and how it allows us to re-signify the appropriated images, questioning visuality as a discourse of progress and emancipationAyuda Predoctoral Conselleria (ACIF/2019/010) con cofinanciación del Fondo Social Europeo.Císcar-Cebria, A. (2021). The act of seeing with no eyes: a videographic project about visuality as an instrument of power. Avanca - Cinema (Online). 2021:259-264. http://hdl.handle.net/10251/186640S259264202

NARRACIÓN Y SABOTAJE

[EN] This project stems from the reflection on how photography and painting are able to create a narrative built, open to mu ltiple readings. Based on production arising pictorial and photographic work by appropriating images of old press, we intend to analyze and to question the veracity of the document image. An appropriate material of the context of reality, but nevertheless, in his painting process acquires another meaning away from the original to be decontextualized and fragmented . It aims to offer the viewer a new and contradictory, ambiguous visual work, giving you the ability to rearrange and rebuild, is precisely the ar rangement and juxtaposition of images allowing that they can finally be read and reread by multiple fictional directions[ES] El presente proyecto nace de la reflex ión sobre cómo la fotografía y la pintura son capaces de crear una narración construida, abierta a múltiples lecturas . En base a una producción de obra pictórica y fotográfica surgida mediante la apropiación de imágenes de prensa antigua , se pretende anali zar y cuestionar la veracidad de la imagen documento. Un material apropiado del contexto de lo real, pero que sin embargo, en su traduc ción pictórica adquiere otro significa do alejado del origina l al estar descontextualizado y fragmen tado. Se pretende o frecer al espectador una obra plástica nueva y contradictoria, ambigua, dándole la posibi lidad de reordenar y reconstruir, sien do precisamente la disposición y yuxtaposición de las imágenes lo que permite que éstas puedan finalmente ser leídas y releídas según múltiples direcciones más cercanas a lo meramente ficcionalCíscar Cebria, A. (2016). NARRACIÓN Y SABOTAJE. http://hdl.handle.net/10251/74426.TFG

Evolution of the EGFR pathway in Metazoa and its diversification in the planarian Schmidtea mediterranea

This study was funded by grants, BFU2012-31701 and BFU2015-65704-P(Ministerio de Economia y Competitividad/Feder (Spain)) and 2009-SGR-1018 from the Generalitat de Catalunya to FC. SB was supported by a FI fellowship from the Generalitat de Catalunya and a collaboration fellowship from IBUB. JMMD was supported by Marie Curie IEF 329024 fellowship and Sars core budg

The TALE Class Homeobox Gene Smed-prep Defines the Anterior Compartment for Head Regeneration

Planaria continue to blossom as a model system for understanding all aspects of regeneration. They provide an opportunity to understand how the replacement of missing tissues from preexisting adult tissue is orchestrated at the molecular level. When amputated along any plane, planaria are capable of regenerating all missing tissue and rescaling all structures to the new size of the animal. Recently, rapid progress has been made in understanding the developmental pathways that control planarian regeneration. In particular Wnt/beta-catenin signaling is central in promoting posterior fates and inhibiting anterior identity. Currently the mechanisms that actively promote anterior identity remain unknown. Here, Smed-prep, encoding a TALE class homeodomain, is described as the first gene necessary for correct anterior fate and patterning during planarian regeneration. Smed-prep is expressed at high levels in the anterior portion of whole animals, and Smed-prep(RNAi) leads to loss of the whole brain during anterior regeneration, but not during lateral regeneration or homeostasis in intact worms. Expression of markers of different anterior fated cells are greatly reduced or lost in Smed-prep(RNAi) animals. We find that the ectopic anterior structures induced by abrogation of Wnt signaling also require Smed-prep to form. We use double knockdown experiments with the S. mediterranea ortholog of nou-darake (that when knocked down induces ectopic brain formation) to show that Smed-prep defines an anterior fated compartment within which stem cells are permitted to assume brain fate, but is not required directly for this differentiation process. Smed-prep is the first gene clearly implicated as being necessary for promoting anterior fate and the first homeobox gene implicated in establishing positional identity during regeneration. Together our results suggest that Smed-prep is required in stem cell progeny as they form the anterior regenerative blastema and is required for specifying anterior cell fates and correct patterning

A Low Percent Ethanol Method for Immobilizing Planarians

Planarians have recently become a popular model system for the study of adult stem cells, regeneration and polarity. The system is attractive for both undergraduate and graduate research labs, since planarian colonies are low cost and easy to maintain. Also in situ hybridization, immunofluorescence and RNA-interference (RNAi) gene knockdown techniques have been developed for planarian studies. However, imaging of live worms (particularly at high magnifications) is difficult because animals are strongly photophobic; they quickly move away from light sources and out of frame. The current methods available to inhibit movement in planarians include RNAi injection and exposure to cold temperatures. The former is labor and time intensive, while the latter precludes the use of many fluorescent reporter dyes. Here, we report a simple, inexpensive and reversible method to immobilize planarians for live imaging. Our data show that a short 1 hour treatment with 3% ethanol (EtOH) is sufficient to inhibit both the fine and gross movements of Schmidtea mediterranea planarians, of the typical size used (4–6 mm), with full recovery of movement within 3–4 hours. Importantly, EtOH treatment did not interfere with regeneration, even after repeated exposure, nor lyse epithelial cells (as assayed by H&E staining). We demonstrate that a short exposure to a low concentration of EtOH is a quick and effective method of immobilizing planarians, one that is easily adaptable to planarians of all sizes and will increase the accessibility of live imaging assays to planarian researchers

Combining Classical and Molecular Approaches Elaborates on the Complexity of Mechanisms Underpinning Anterior Regeneration

The current model of planarian anterior regeneration evokes the establishment of low levels of Wnt signalling at anterior wounds, promoting anterior polarity and subsequent elaboration of anterior fate through the action of the TALE class homeodomain PREP. The classical observation that decapitations positioned anteriorly will regenerate heads more rapidly than posteriorly positioned decapitations was among the first to lead to the proposal of gradients along an anteroposterior (AP) axis in a developmental context. An explicit understanding of this phenomenon is not included in the current model of anterior regeneration. This raises the question what the underlying molecular and cellular basis of this temporal gradient is, whether it can be explained by current models and whether understanding the gradient will shed light on regenerative events. Differences in anterior regeneration rate are established very early after amputation and this gradient is dependent on the activity of Hedgehog (Hh) signalling. Animals induced to produce two tails by either Smed-APC-1(RNAi) or Smed-ptc(RNAi) lose anterior fate but form previously described ectopic anterior brain structures. Later these animals form peri-pharyngeal brain structures, which in Smed-ptc(RNAi) grow out of the body establishing a new A/P axis. Combining double amputation and hydroxyurea treatment with RNAi experiments indicates that early ectopic brain structures are formed by uncommitted stem cells that have progressed through S-phase of the cell cycle at the time of amputation. Our results elaborate on the current simplistic model of both AP axis and brain regeneration. We find evidence of a gradient of hedgehog signalling that promotes posterior fate and temporarily inhibits anterior regeneration. Our data supports a model for anterior brain regeneration with distinct early and later phases of regeneration. Together these insights start to delineate the interplay between discrete existing, new, and then later homeostatic signals in AP axis regeneration

Post-collisional Tertiary–Quaternary mafic alkalic magmatism in the Carpathian–Pannonian region: a review

Mafic alkalic volcanism was widespread in the Carpathian–Pannonian region (CPR) between 11 and 0.2 Ma. It followed the Miocene continental collision of the Alcapa and Tisia blocks with the European plate, as subduction-related calc-alkaline magmatism was waning. Several groups of mafic alkalic rocks from different regions within the CPR have been distinguished on the basis of ages and/or trace-element compositions. Their trace element and Sr–Nd–Pb isotope systematics are consistent with derivation from complex mantle-source regions, which included both depleted asthenosphere and metasomatized lithosphere. The mixing of DMM-HIMU-EMII mantle components within asthenosphere-derived magmas indicates variable contamination of the shallow asthenosphere and/or thermal boundary layer of the lithosphere by a HIMU-like component prior to and following the introduction of subduction components. Various mantle sources have been identified: Lower lithospheric mantle modified by several ancient asthenospheric enrichments (source A); Young asthenospheric plumes with OIB-like trace element signatures that are either isotopically enriched (source B) or variably depleted (source C); Old upper asthenosphere heterogeneously contaminated by DM-HIMU-EMII-EMI components and slightly influenced by Miocene subduction-related enrichment (source D); Old upper asthenosphere heterogeneously contaminated by DM-HIMU-EMII components and significantly influenced by Miocene subduction-related enrichment (source E). Melt generation was initiated either by: (i) finger-like young asthenospheric plumes rising to and heating up the base of the lithosphere (below the Alcapa block), or (ii) decompressional melting of old asthenosphere upwelling to replace any lower lithosphere or heating and melting former subducted slabs (the Tisia block)

In utero undernutrition in male mice programs liver lipid metabolism in the second-generation offspring involving altered lxra DNA methylation

SummaryObesity and type 2 diabetes have a heritable component that is not attributable to genetic factors. Instead, epigenetic mechanisms may play a role. We have developed a mouse model of intrauterine growth restriction (IUGR) by in utero malnutrition. IUGR mice developed obesity and glucose intolerance with aging. Strikingly, offspring of IUGR male mice also developed glucose intolerance. Here, we show that in utero malnutrition of F1 males influenced the expression of lipogenic genes in livers of F2 mice, partly due to altered expression of Lxra. In turn, Lxra expression is attributed to altered DNA methylation of its 5′ UTR region. We found the same epigenetic signature in the sperm of their progenitors, F1 males. Our data indicate that in utero malnutrition results in epigenetic modifications in germ cells (F1) that are subsequently transmitted and maintained in somatic cells of the F2, thereby influencing health and disease risk of the offspring