42 research outputs found

    Guía práctica de la educación digital

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    En esta guía se ofrece recomendaciones pedagógicas sobre el uso educativo de las tabletas en el aula apoyadas en la investigación del proyecto Samsung Smart School. Por un lado, se busca conocer cómo es la práctica educativa con tabletas en las aulas en el que la aplica el proyecto (cómo es) y, desde esta evidencia, proponer cómo se podría mejorar y potenciar esa práctica a través de una serie de recomendaciones pedagógicas (cómo puede ser). La estructura es: Prólogo de Francesc Pedró (UNESCO) Cap. 1: A modo de introducción Cap. 2: Ecosistema educativo con tecnología: “Samsung Smart School” Cap. 3: “Samsung Smart School” desde la práctica docente Cap. 4: Construcción del aula con tecnología. Recomendaciones desde “Samsung Smart School” Cap. 5: Lo que hemos aprendido en “Samsung Smart School” Anexo: Ejemplos de integración curricula

    Un nuevo híbrido del género Ophrys L. (Orchidaceae) en el litoral valenciano

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    [EN] Here we describe a new hybrid of genus Ophrys L. (Orchidaceae) found in Cullera (Valencia): Ophrys x donatiae, which comes from the crossing between Ophrys dianica and O. tenthredinifera, and has intermediate characters. Distribution area and phenology are shared by parental plants.[ES] Se describe un nuevo híbrido del género Ophrys L. (Orchidaceae) encontrado en Cullera (Valencia): Ophrys x donatiae. Es el resultado del cruce entre O. dianica y O. tenthredinifera, mostrando caracteres intermedios. El área de distribución de los parentales y la fenología son coincidentes.Tejedor, P.; Català, S.; Sospedra, J. (2012). Un nuevo híbrido del género Ophrys L. (Orchidaceae) en el litoral valenciano. Flora Montiberica. 51:93-96. http://hdl.handle.net/10251/36441S93965

    Campylobacter epidemiology from breeders to their progeny in Eastern Spain

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    [EN] While horizontal transmission is a route clearly linked to the spread of Campylobacter at the farm level, few studies support the transmission of Campylobacter spp. from breeder flocks to their offspring. Thus, the present study was carried out to investigate the possibility of vertical transmission. Breeders were monitored from the time of housing day-old chicks, then throughout the laying period (0 to 60 wk) and throughout their progeny (broiler fattening, 1 to 42 d) until slaughter. All samples were analyzed according with official method ISO 10272:2006. Results revealed that on breeder farms, Campylobacter isolation started from wk 16 and reached its peak at wk 26, with 57.0% and 93.2% of positive birds, respectively. After this point, the rate of positive birds decreased slightly to 86.0% at 60 wk. However, in broiler production all day-old chicks were found negative for Campylobacter spp, and the bacteria was first isolated at d 14 of age (5.0%), with a significant increase in detection during the fattening period with 62% of Campylobacter positive animals at the end of the production cycle. Moreover, non-positive sample was determined from environmental sources. These results could be explained because Campylobacter may be in a low concentration or in a non-culturable form, as there were several studies that successfully detected Campylobacter DNA, but failed to culture. This form can survive in the environment and infect successive flocks; consequently, further studies are needed to develop more modern, practical, cost-effective and suitable techniques for routine diagnosis.We thank the ASAV (Valencian Poultry Association) and the Santander bank for the financial support of the project and the staff of the CECAV (Center for Poultry and Animal Feed Quality of the Valencian Region) for their assistance. Sofia Ingresa was supported by a research grant from the Spanish Ministry of Education (programme FPU13/03306). English text version was revised by N. Macowan English Language Service.Ingresa-Capaccioni, S.; Jiménez Trigos, ME.; Marco Jiménez, F.; Català, P.; Vega García, S.; Marin Orenga, C. (2016). Campylobacter epidemiology from breeders to their progeny in Eastern Spain. Poultry Science. 95(3):676-683. https://doi.org/10.3382/ps/pev338S67668395

    High-throughput amplicon sequencing-based analysis of active fungal communities inhabiting grapevine after hot-water treatments reveals unexpectedly high fungal diversity

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    [EN] The ecology of total fungal communities in grapevine is so far largely derived from studies on culture-dependent methods or cultivation-independent rDNA approaches. Sequencing the ribosomal RNA transcripts (rRNA) would rather reveal the functionally and metabolically active important taxa of the fungal community and provide insights into its activity in the wood. The present study investigated changes in the potentially active fungal communities of internal grapevine wood after Hot-Water Treatment (HWT) in planting material from Czech Republic and Spain at two different times during the propagation process and from two plant zones. We examined fungal communities using both traditional isolation into culture and high-throughput amplicon sequencing (HTAS) of the internal transcribed spacer 2 (ITS2) region in extracted total RNA. HTAS from metatranscriptomic RNA increased the resolution of the fungal community analysis and revealed a highly diverse mycobiota of grapevine wood compared to the traditional method. Fungal diversity differed between grapevine genotypes and showed a temporal variation over the vegetative period. Grapevine planting materials exhibited high fungal diversity after HWT, which demonstrates that the HWT process does not sterilize the internal wood of grapevine. HWT reduced the infection caused by fungal trunk disease pathogens but was not completely effective in eliminating their growth. This study provides important and practically useful insights into the dynamics of active fungal communities in hot-water treated plants, and represents the first study of active fungal communities on grapevine grafted plants by comparing traditional and next-generation sequencing methods. (C) 2018 Elsevier Ltd and British Mycological Society. All rights reserved.This work was supported by Projects No. LD14051 from the Ministry of Education, Youth and Sports, Czech Republic covered by COST Action FA1303, and No. CZ.02.1.01/0.0/0.0/16_025/0007314 "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" from EFRR (Grant provider: Ministerstvo Skolstvi, mladeze a telovychovy Ceske republiky (MSMT). Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the programme "Projects of Large Research, Development, and Innovations Infrastructures" (CESNET LM2015042), is greatly appreciated. David Gramaje was supported by the DOC-INIA program from the National Institute for Agronomic Research (INIA), co-funded by the European Social Fund. The authors are grateful for the technical assistance of Jesus Alfonso Crespo and Aitor Gavara Vidal.Eichmeier, A.; Pecenka, J.; Penazova, E.; Baranek, M.; Català, S.; León Santana, M.; Armengol Fortí, J.... (2018). High-throughput amplicon sequencing-based analysis of active fungal communities inhabiting grapevine after hot-water treatments reveals unexpectedly high fungal diversity. Fungal Ecology. 36:26-38. https://doi.org/10.1016/j.funeco.2018.07.011S26383

    Characterization of Mycosphaerellaceae species associated with citrus greasy spot in Panama and Spain

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    [EN] Greasy spot of citrus, caused by Zasmidium citri-griseum (= Mycosphaerella citri), is widely distributed in the Caribbean Basin, inducing leaf spots, premature defoliation, and yield loss. Greasy spot-like symptoms were frequently observed in humid citrus-growing regions in Panama as well as in semi-arid areas in Spain, but disease aetiology was unknown. Citrus-growing areas in Panama and Spain were surveyed and isolates of Mycosphaerellaceae were obtained from citrus greasy spot lesions. A selection of isolates from Panama (n = 22) and Spain (n = 16) was assembled based on their geographical origin, citrus species, and affected tissue. The isolates were characterized based on multi-locus DNA (ITS and EF-1 alpha) sequence analyses, morphology, growth at different temperatures, and independent pathogenicity tests on the citrus species most affected in each country. Reference isolates and sequences were also included in the analysis. Isolates from Panama were identified as Z. citri-griseum complex, and others from Spain attributed to Amycosphaerella africana. Isolates of the Z. citri-griseum complex had a significantly higher optimal growth temperature (26.8 degrees C) than those of A. africana (19.3 degrees C), which corresponded well with their actual biogeographical range. The isolates of the Z. citri-griseum complex from Panama induced typical greasy spot symptoms in 'Valencia' sweet orange plants and the inoculated fungi were reisolated. No symptoms were observed in plants of the 'Ortanique' tangor inoculated with A. africana. These results demonstrate the presence of citrus greasy spot, caused by Z. citri-griseum complex, in Panama whereas A. africana was associated with greasy spot-like symptoms in Spain.Research was partially funded by 'Programa de Formacion de los INIA Iberoamerica' and INIA RTA2010-00105-00-00-FEDER to Vidal Aguilera Cogley.. We thank J. Martinez-Minaya (UV) for assistance with INLAAguilera-Cogley, VA.; Berbegal Martinez, M.; Català, S.; Collison Brentu, F.; Armengol Fortí, J.; Vicent Civera, A. (2017). Characterization of Mycosphaerellaceae species associated with citrus greasy spot in Panama and Spain. PLoS ONE. 12(12):1-19. https://doi.org/10.1371/journal.pone.0189585S1191212Crous, P. W., Summerell, B. A., Carnegie, A. J., Wingfield, M. J., Hunter, G. C., Burgess, T. I., … Groenewald, J. Z. (2009). Unravelling Mycosphaerella: do you believe in genera? Persoonia - Molecular Phylogeny and Evolution of Fungi, 23(1), 99-118. doi:10.3767/003158509x479487Mondal, S. N., & Timmer, L. W. (2006). Greasy Spot, a Serious Endemic Problem for Citrus Production in the Caribbean Basin. Plant Disease, 90(5), 532-538. doi:10.1094/pd-90-0532Whiteside, J. O. (1970). Etiology and Epidemiology of Citrus Greasy Spot. Phytopathology, 60(10), 1409. doi:10.1094/phyto-60-1409Huang, F., Groenewald, J. Z., Zhu, L., Crous, P. W., & Li, H. (2015). Cercosporoid diseases of Citrus. Mycologia, 107(6), 1151-1171. doi:10.3852/15-059Wellings, C. R. (1981). Pathogenicity of fungi associated with citrus greasy spot in New South Wales. Transactions of the British Mycological Society, 76(3), 495-499. doi:10.1016/s0007-1536(81)80080-0Marco, G. M. (1986). A Disease Similar to Greasy Spot but of Unknown Etiology on Citrus Leaves in Argentina. Plant Disease, 70(11), 1074a. doi:10.1094/pd-70-1074aVidal Aguilera-Cogley, & Antonio Vicent. (2015). FUNGAL DISEASES OF CITRUS IN PANAMA. Acta Horticulturae, (1065), 947-952. doi:10.17660/actahortic.2015.1065.118Honger J. Aetiology and importance of foliage diseases affecting citrus in the nursery at the Agricultural Research Station (ARS). PhD Thesis. Accra: University of Ghana; 2004.Vicent A, Álvarez A, León M, García-Jiménez J. Mycosphaerella sp. asociada a manchas foliares de cítricos en España. In: Proceedings of the 13th Congress of the Spanish Phytopathological Society. 2006; Murcia; Spain.Abdelfattah, A., Cacciola, S. O., Mosca, S., Zappia, R., & Schena, L. (2016). Analysis of the Fungal Diversity in Citrus Leaves with Greasy Spot Disease Symptoms. Microbial Ecology, 73(3), 739-749. doi:10.1007/s00248-016-0874-xQuaedvlieg, W., Binder, M., Groenewald, J. Z., Summerell, B. A., Carnegie, A. J., Burgess, T. I., & Crous, P. W. (2014). Introducing the Consolidated Species Concept to resolve species in the Teratosphaeriaceae. Persoonia - Molecular Phylogeny and Evolution of Fungi, 33(1), 1-40. doi:10.3767/003158514x681981Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5), 1792-1797. doi:10.1093/nar/gkh340Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8), 772-772. doi:10.1038/nmeth.2109Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D. L., Darling, A., Höhna, S., … Huelsenbeck, J. P. (2012). MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61(3), 539-542. doi:10.1093/sysbio/sys029Rambaut A. FigTree v1. 4.0, a graphical viewer of phylogenetic trees. Edinburgh, Scotland: University of Edinburgh; 2016.Spiegelhalter, D. J., Best, N. G., Carlin, B. P., & van der Linde, A. (2002). Bayesian measures of model complexity and fit. Journal of the Royal Statistical Society: Series B (Statistical Methodology), 64(4), 583-639. doi:10.1111/1467-9868.00353Rue, H., Martino, S., & Chopin, N. (2009). Approximate Bayesian inference for latent Gaussian models by using integrated nested Laplace approximations. Journal of the Royal Statistical Society: Series B (Statistical Methodology), 71(2), 319-392. doi:10.1111/j.1467-9868.2008.00700.xChristensen RH. Ordinal—regression models for ordinal data. R package version 2015.1–21. 2015. http://www.cran.r-project.org/package=ordinal/ Accessed 8 May 2017.Hunter, G. C., Wingfield, B. D., Crous, P. W., & Wingfield, M. J. (2006). A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves. Studies in Mycology, 55, 147-161. doi:10.3114/sim.55.1.147Braun, U., & Urtiaga, R. (2013). New species and new records of cercosporoid hyphomycetes from Cuba and Venezuela (Part 2). Mycosphere, 4(2), 172-214. doi:10.5943/mycosphere/4/2/3Braun, U., Crous, P. W., & Nakashima, C. (2014). Cercosporoid fungi (Mycosphaerellaceae) 2. Species on monocots (Acoraceae to Xyridaceae, excluding Poaceae). IMA Fungus, 5(2), 203-390. doi:10.5598/imafungus.2014.05.02.04Aptroot A. Mycosphaerella and its anamorphs: conspectus of Mycosphaerella CBS Biodiversity Series 5. Utrecht: CBS-KNAW Fungal Biodiversity Centre; 2006.Crous, P. W., & Wingfield, M. J. (1996). Species of Mycosphaerella and Their Anamorphs Associated with Leaf Blotch Disease of Eucalyptus in South Africa. Mycologia, 88(3), 441. doi:10.2307/3760885Aguín, O., Sainz, M. J., Ares, A., Otero, L., & Pedro Mansilla, J. (2013). Incidence, severity and causal fungal species of Mycosphaerella and Teratosphaeria diseases in Eucalyptus stands in Galicia (NW Spain). Forest Ecology and Management, 302, 379-389. doi:10.1016/j.foreco.2013.03.021Maxwell, A., Dell, B., Neumeister-Kemp, H. G., & Hardy, G. E. S. J. (2003). Mycosphaerella species associated with Eucalyptus in south-western Australia: new species, new records and a key. Mycological Research, 107(3), 351-359. doi:10.1017/s0953756203007354Otero L, Aguín O, Mansilla J, Hunter G, Wingfield M. Identificación de especies de Mycosphaerella en Eucalyptus globulus y E. nitens en Galicia. In: Proceedings of the 13th Congress of the Spanish Phytopathological Society; 2006; Murcia, Spain.ZHAN, J., & McDONALD, B. A. (2011). Thermal adaptation in the fungal pathogen Mycosphaerella graminicola. Molecular Ecology, 20(8), 1689-1701. doi:10.1111/j.1365-294x.2011.05023.xPeel, M. C., Finlayson, B. L., & McMahon, T. A. (2007). Updated world map of the Köppen-Geiger climate classification. Hydrology and Earth System Sciences, 11(5), 1633-1644. doi:10.5194/hess-11-1633-200

    Detectable clonal mosaicism in blood as a biomarker of cancer risk in Fanconi anemia

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    Detectable clonal mosaicism for large chromosomal events has been associated with aging and an increased risk of hematological and some solid cancers. We hypothesized that genetic cancer predisposition disorders, such as Fanconi anemia (FA), could manifest a high rate of chromosomal mosaic events (CMEs) in peripheral blood, which could be used as early biomarkers of cancer risk. We studied the prevalence of CMEs by single-nucleotide polymorphism (SNP) array in 130 FA patients' blood DNA and their impact on cancer risk. We detected 51 CMEs (4.4-159 Mb in size) in 16 out of 130 patients (12.3%), of which 9 had multiple CMEs. The most frequent events were gains at 3q (n = 6) and 1q (n = 5), both previously associated with leukemia, as well as rearrangements with breakpoint clustering within the major histocompatibility complex locus (P = 7.3 x 10(-9)). Compared with 15 743 age-matched population controls, FA patients had a 126 to 140 times higher risk of detectable CMEs in blood (P < 2.2 x 10(-16)). Prevalent and incident hematologic and solid cancers were more common in CME carriers (odds ratio [OR] = 11.6, 95% confidence interval [CI] = 3.4-39.3, P = 2.8 x 10(-5)), leading to poorer prognosis. The age-adjusted hazard risk (HR) of having cancer was almost 5 times higher in FA individuals with CMEs than in those without CMEs. Regarding survival, the HR of dying was 4 times higher in FA individuals having CMEs (HR = 4.0, 95% CI = 2.0-7.9, P = 5.7 x 10(-5)). Therefore, our data suggest that molecular karyotyping with SNP arrays in easy-to-obtain blood samples could be used for better monitoring of bone marrow clonal events, cancer risk, and overall survival of FA patients

    The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain

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    [EN] Phytophthora is one of the most important and aggressive plant pathogenic genera in agriculture and forestry. Early detection and identification of its pathways of infection and spread are of high importance to minimize the threat they pose to natural ecosystems. eDNA was extracted from soil and water from forests and plantations in the north of Spain. Phytophthora-specific primers were adapted for use in high-throughput Sequencing (HTS). Primers were tested in a control reaction containing eight Phytophthora species and applied to water and soil eDNA samples from northern Spain. Different score coverage threshold values were tested for optimal Phytophthora species separation in a custom-curated database and in the control reaction. Clustering at 99% was the optimal criteria to separate most of the Phytophthora species. Multiple Molecular Operational Taxonomic Units (MOTUs) corresponding to 36 distinct Phytophthora species were amplified in the environmental samples. Pyrosequencing of amplicons from soil samples revealed low Phytophthora diversity (13 species) in comparison with the 35 species detected in water samples. Thirteen of the MOTUs detected in rivers and streams showed no close match to sequences in international sequence databases, revealing that eDNA pyrosequencing is a useful strategy to assess Phytophthora species diversity in natural ecosystems.This project has been supported by the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (EUPHRESCO-CEP: "Current and Emerging Phytophthoras: Research Supporting Risk Assessment And Risk Management"). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Català, S.; Pérez Sierra, AM.; Abad Campos, P. (2015). The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain. PLoS ONE. 10(3):1-14. doi:10.1371/journal.pone.0119311S114103REICHARD, S. H., & WHITE, P. (2001). Horticulture as a Pathway of Invasive Plant Introductions in the United States. BioScience, 51(2), 103. doi:10.1641/0006-3568(2001)051[0103:haapoi]2.0.co;2Brasier, C. M. (2008). The biosecurity threat to the UK and global environment from international trade in plants. Plant Pathology, 57(5), 792-808. doi:10.1111/j.1365-3059.2008.01886.xTABERLET, P., COISSAC, E., HAJIBABAEI, M., & RIESEBERG, L. H. (2012). Environmental DNA. Molecular Ecology, 21(8), 1789-1793. doi:10.1111/j.1365-294x.2012.05542.xSogin, M. L., Morrison, H. G., Huber, J. A., Welch, D. M., Huse, S. M., Neal, P. R., … Herndl, G. J. (2006). Microbial diversity in the deep sea and the underexplored «rare biosphere». Proceedings of the National Academy of Sciences, 103(32), 12115-12120. doi:10.1073/pnas.0605127103Roesch, L. F. W., Fulthorpe, R. R., Riva, A., Casella, G., Hadwin, A. K. M., Kent, A. D., … Triplett, E. W. (2007). Pyrosequencing enumerates and contrasts soil microbial diversity. The ISME Journal, 1(4), 283-290. doi:10.1038/ismej.2007.53Acosta-Martínez, V., Dowd, S., Sun, Y., & Allen, V. (2008). Tag-encoded pyrosequencing analysis of bacterial diversity in a single soil type as affected by management and land use. Soil Biology and Biochemistry, 40(11), 2762-2770. doi:10.1016/j.soilbio.2008.07.022Jumpponen, A., & Jones, K. L. (2009). Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperateQuercus macrocarpaphyllosphere. New Phytologist, 184(2), 438-448. doi:10.1111/j.1469-8137.2009.02990.xNilsson, R. H., Ryberg, M., Abarenkov, K., Sjökvist, E., & Kristiansson, E. (2009). The ITS region as a target for characterization of fungal communities using emerging sequencing technologies. FEMS Microbiology Letters, 296(1), 97-101. doi:10.1111/j.1574-6968.2009.01618.xCoince, A., Caël, O., Bach, C., Lengellé, J., Cruaud, C., Gavory, F., … Buée, M. (2013). Below-ground fine-scale distribution and soil versus fine root detection of fungal and soil oomycete communities in a French beech forest. Fungal Ecology, 6(3), 223-235. doi:10.1016/j.funeco.2013.01.002Vannini, A., Bruni, N., Tomassini, A., Franceschini, S., & Vettraino, A. M. (2013). Pyrosequencing of environmental soil samples reveals biodiversity of thePhytophthoraresident community in chestnut forests. FEMS Microbiology Ecology, 85(3), 433-442. doi:10.1111/1574-6941.12132Jerde, C. L., Mahon, A. R., Chadderton, W. L., & Lodge, D. M. (2011). «Sight-unseen» detection of rare aquatic species using environmental DNA. Conservation Letters, 4(2), 150-157. doi:10.1111/j.1755-263x.2010.00158.xMonchy, S., Sanciu, G., Jobard, M., Rasconi, S., Gerphagnon, M., Chabé, M., … Sime-Ngando, T. (2011). Exploring and quantifying fungal diversity in freshwater lake ecosystems using rDNA cloning/sequencing and SSU tag pyrosequencing. Environmental Microbiology, 13(6), 1433-1453. doi:10.1111/j.1462-2920.2011.02444.xJobard, M., Rasconi, S., Solinhac, L., Cauchie, H.-M., & Sime-Ngando, T. (2012). Molecular and morphological diversity of fungi and the associated functions in three European nearby lakes. Environmental Microbiology, 14(9), 2480-2494. doi:10.1111/j.1462-2920.2012.02771.xLivermore, J. A., & Mattes, T. E. (2013). Phylogenetic detection of novel Cryptomycota in an Iowa (United States) aquifer and from previously collected marine and freshwater targeted high-throughput sequencing sets. Environmental Microbiology, 15(8), 2333-2341. doi:10.1111/1462-2920.12106NAKAYAMA, J., JIANG, J., WATANABE, K., CHEN, K., NINXIN, H., MATSUDA, K., … LEE, Y.-K. (2013). Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing. Bioscience of Microbiota, Food and Health, 32(2), 69-76. doi:10.12938/bmfh.32.69CREER, S., & SINNIGER, F. (2012). Cosmopolitanism of microbial eukaryotes in the global deep seas. Molecular Ecology, 21(5), 1033-1035. doi:10.1111/j.1365-294x.2012.05437.xDavey, M. L., Heegaard, E., Halvorsen, R., Kauserud, H., & Ohlson, M. (2012). Amplicon-pyrosequencing-based detection of compositional shifts in bryophyte-associated fungal communities along an elevation gradient. Molecular Ecology, 22(2), 368-383. doi:10.1111/mec.12122Weber, C. F., Vilgalys, R., & Kuske, C. R. (2013). Changes in Fungal Community Composition in Response to Elevated Atmospheric CO2 and Nitrogen Fertilization Varies with Soil Horizon. Frontiers in Microbiology, 4. doi:10.3389/fmicb.2013.00078Bergmark, L., Poulsen, P. H. B., Al-Soud, W. A., Norman, A., Hansen, L. H., & Sørensen, S. J. (2012). Assessment of the specificity of Burkholderia and Pseudomonas qPCR assays for detection of these genera in soil using 454 pyrosequencing. FEMS Microbiology Letters, 333(1), 77-84. doi:10.1111/j.1574-6968.2012.02601.xLi, L., Abu Al-Soud, W., Bergmark, L., Riber, L., Hansen, L. H., Magid, J., & Sørensen, S. J. (2013). Investigating the Diversity of Pseudomonas spp. in Soil Using Culture Dependent and Independent Techniques. Current Microbiology, 67(4), 423-430. doi:10.1007/s00284-013-0382-xSCHENA, L., HUGHES, K. J. D., & COOKE, D. E. L. (2006). Detection and quantification ofPhytophthora ramorum,P. kernoviae,P. citricolaandP. quercinain symptomatic leaves by multiplex real-time PCR. Molecular Plant Pathology, 7(5), 365-379. doi:10.1111/j.1364-3703.2006.00345.xTooley, P. W., Martin, F. N., Carras, M. M., & Frederick, R. D. (2006). Real-Time Fluorescent Polymerase Chain Reaction Detection ofPhytophthora ramorumandPhytophthora pseudosyringaeUsing Mitochondrial Gene Regions. Phytopathology, 96(4), 336-345. doi:10.1094/phyto-96-0336Pavón, C. F., Babadoost, M., & Lambert, K. N. (2008). Quantification of Phytophthora capsici Oospores in Soil by Sieving-Centrifugation and Real-Time Polymerase Chain Reaction. Plant Disease, 92(1), 143-149. doi:10.1094/pdis-92-1-0143Than, D. J., Hughes, K. J. D., Boonhan, N., Tomlinson, J. A., Woodhall, J. W., & Bellgard, S. E. (2013). A TaqMan real-time PCR assay for the detection ofPhytophthora‘taxon Agathis’ in soil, pathogen of Kauri in New Zealand. Forest Pathology, 43(4), 324-330. doi:10.1111/efp.12034Chen, W., Djama, Z. R., Coffey, M. D., Martin, F. N., Bilodeau, G. J., Radmer, L., … Lévesque, C. A. (2013). Membrane-Based Oligonucleotide Array Developed from Multiple Markers for the Detection of Many Phytophthora Species. Phytopathology, 103(1), 43-54. doi:10.1094/phyto-04-12-0092-rScibetta, S., Schena, L., Chimento, A., Cacciola, S. O., & Cooke, D. E. L. (2012). A molecular method to assess Phytophthora diversity in environmental samples. 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    Els bolets de la Safor i zones limítrofes

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    Un repàs per la diversitat fúngica de la Safor i les zones limítrofes. Conté una fitxa per a cada espècie on es troba el nom científic i vulgar, descripció, comestibilitat, hàbitat i observacions, junt a una foto i un mapa de localització.Un repàs per la diversitat fúngica de la Safor i les zones limítrofes. Conté una fitxa per a cada espècie on es troba el nom científic i vulgar, descripció, comestibilitat, hàbitat i observacions, junt a una foto i un mapa de localització.García Català, S. (2010). Els bolets de la Safor i zones limítrofes. CEIC Alfons El Vell. http://hdl.handle.net/10251/8545

    Percepción docente sobre la transformación digital del aula a través de tabletas: un estudio en el contexto español

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    This study examines the transformation of classroom dynamics brought about by the use of tablets for educational purposes. The empirical bases of this study were defined by the “Samsung Smart School” project, which was developed by Samsung and Spain’s Ministry of Education during academic year 2014-15, in which teachers and 5th and 6th year students attending 15 primary schools across several Autonomous Communities in Spain were provided with tablets. The research sample comprised 166 teachers. A qualitative analysis strategy was applied by means of: a) non-participant observation, b) focus groups, c) semi-structured interviews with teachers, and d) content analysis of teaching units. These techniques enabled us to extract and examine six dimensions of teaching (educational objective, teaching approach, organization of content and activities, teaching resources, space and time, and learning assessment). Our findings show that teachers tend to apply a transversal approach when using tablets to work on different competencies, focusing more on activities than on content through the use of apps. They reclaim the act of play as part of the learning process, and tablet use encourages project-based learning. To sum up, this study shows that teachers view tablets not only as a technological challenge, but also as an opportunity to rethink their traditional teaching models.El presente estudio examina la trasformación de la dinámica del aula a través del uso educativo de las tabletas. La base empírica de este estudio se enmarca en el proyecto «Samsung Smart School», desarrollado entre Samsung y el Ministerio de Educación de España en el curso 2014-15. Se dotó de tabletas a profesores y alumnos de aulas de 5º y 6º de primaria de 15 centros de Educación Primaria de distintas comunidades autónomas del territorio Español. En suma el estudio se llevó a cabo con una muestra comprendida por 166 docentes. Se empleó una estrategia analítica cualitativa mediante: a) observación no participante, b) grupos focales, c) entrevistas semiestructuradas al profesorado y d) análisis de contenido de unidades didácticas. Dichas técnicas permitieron abordar el estudio de seis dimensiones pedagógicas (finalidad educativa, enfoque pedagógico, organización de contenidos y actividades, recursos didácticos, espacio y tiempo y evaluación del aprendizaje). Los hallazgos evidencian la tendencia del profesorado a trabajar con tabletas de forma transversal distintas competencias, centrarse en las actividades más que el contenido a través de las apps, asumir el reto de recuperar el juego como parte del aprendizaje y poner en práctica el aprendizaje basado en proyectos. En suma, la principal evidencia es que los docentes entienden la tableta no solo como un reto tecnológico, sino como la oportunidad para repensar sus modelos pedagógicos tradicionales
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