Optimizing CRISPR/Cas9 for high-expression genome loci in industrial yeast strains

Book of Abstracts of CEB Annual Meeting 2017[Excerpt] The yeast Saccharomyces cerevisiae is one of the key cell factories for the production of bio-based chemicals, from fuels and bulk chemicals to active pharmaceuticals. Generally recognized as safe (GRAS) by the U. S. Food and Drug Administration and with a broad array of tools available at the molecular level, S. cerevisiae has been successfully manipulated for a wide range of applications. For large-scale fermentations, particularly in biorefineries, yeast cells must perform under harsh conditions, such as fluctuating pH and temperature, high osmotic pressure and presence of inhibitors derived from biomass hydrolysis. In this context, robust and stress-tolerant yeast chassis are required to attain high titers and product yields [1]. Industrial environments have been identified as a bioresource of yeast strains with higher robustness and fermentation performance and distinct strains have been isolated. [...]info:eu-repo/semantics/publishedVersio

Order-preserving reflectors and injectivity

AbstractWe investigate a Galois connection in poset enriched categories between subcategories and classes of morphisms, given by means of the concept of right-Kan injectivity, and, specially, we study its relationship with a certain kind of subcategories, the KZ-reflective subcategories. A number of well-known properties concerning orthogonality and full reflectivity can be seen as a particular case of the ones of right-Kan injectivity and KZ-reflectivity. On the other hand, many examples of injectivity in poset enriched categories encountered in the literature are closely related to the above connection. We give several examples and show that some known subcategories of the category of T0-topological spaces are right-Kan injective hulls of a finite subcategory

Tabaco e a patologia neoplásica pulmonar

The necessity of a tourist and recreative planning concerted on both sides of a frontier, in an area in which the standards of living are in the main relatively high is specially obvious when important distortions incite the particularly developed sectors to extend their influence over other ones less equited quantitatively and qualitatively. This phenomenon, closely linked to the differences in general economic structures, is followed today by social reactions in which the very nature of the development is reflected upon, specially in the weakest regions where the central constituted authorities are led to re-examine their policy, both as regards their support to the peripheral zones and their conception of the spatial planning. But the belated elaboration of a total action renders the solution difficult in a system in which the many and mighty private interests put tight pressures in the matter of real estates and transferable securities. The case of the upper Rhine lands is eloquent in that respect.La nécessité d'un aménagement touristique et récréatif interfrontalier dans un espace à niveaux de vie relativement élevés dans leur ensemble, apparaît surtout là où d'importantes distorsions incitent les secteurs particulièrement développés à étendre leur influence sur les secteurs moins équipés quantitativement et qualitativement. Ce phénomène, étroitement lié aux différences de structures économiques générales, s'accompagne aujourd'hui de réactions sociales qui mettent en cause la nature même du développement, surtout dans les régions les plus faibles, dont les pouvoirs publics centraux sont conduits à réviser leur politique, tant au point de vue du soutien aux zones périphériques qu'à celui de la conception de l'aménagement spatial. Mais l'élaboration tardive d'une action globale rend les solutions difficiles dans un système à l'intérieur duquel les intérêts privés, nombreux et puissants, exercent de fortes pressions foncières et mobilières. Le cas des pays du Rhin Supérieur est éloquent à cet égard.Wackermann Gabriel. La frontière, source de préoccupations d'aménagement touristique et récréatif dans les pays du Rhin Supérieur. In: Hommes et Terres du Nord, 1977/2. pp. 101-110

The proposed acquisition of Danone by Unilever

The purpose of this dissertation is to propose an acquisition in the Consumer Goods Sector. This Sector has been consistently growing in the last years, and Investment Analysts forecast it as one of the fastest moving in the next years. The proposed companies are Unilever as acquirer and Danone as target. Danone is a unique opportunity for Unilever to acquire a company that has brands loved by million families across countries and a strong pathway for delivering long term sustainable value to the economy and environment. Unilever and Danone have an intrinsic value of €123 046million and €44 507million, respectively. The acquisition would follow a friendly takeover with a bid price of €56 265 million, which represents a 30% premium over the market capitalization of the target as of 29th May 2020. The offer would be 80% cash and 20% equity, financed by cash reserves and the issuance of debt. In term of shares, Unilever would need to issue 242million shares for the acquisition. It is expected that the proposed acquisition yield €13 967million in synergies and deliver €2 208 million value to acquirer shareholders.Esta dissertação tem como objetivo propor uma aquisição no setor de Bens de Consumo. Este setor tem vindo a crescer nos últimos anos, e analistas preveem como um dos setores com maior mudança nos próximos anos. Unilever é a empresa proposta como investidora e Danone como empresa adquirida. Danone é uma oportunidade única para Unilever adquirir uma empresa que tem marcas amadas por milhões de famílias em vários países e um plano forte para criar valor sustentável a longo prazo para a economia e ambiente. Unilever e Danone têm valor intrínseco de €123 046 milhões e €44 507 milhões, respetivamente. A aquisição seguiria uma proposta amigável com uma oferta de €56 265 milhões, que representa um prémio de 30% acima do valor de mercado da empresa adquirida a 29 Maio 2020. A oferta seria em dinheiro e ações, financiada por capital próprio e emissão de dívida. Unilever emitiria 242 milhões de novas ações. Espera-se que a aquisição proposta gere sinergias no valor de €13 967m e crie um valor capturado de €2 208 milhões para os acionistas da Unilever

Suitable tools for entrepreneurs manage open innovation: the case of matchmaking in water sector

Trabalho apresentado em 9.ª Conferência Ibérica de Empreendedorismo – CIEM 19, 14-15 novembro, Porto, PortugalN/

Equity research - Semapa - Sociedade de Investimento e Gestão SGPS, S.A.

Mestrado em FinançasEste Trabalho Final de Mestrado pretende apresentar uma avaliação da empresa Semapa - Sociedade de Investimento e Gestão, SGPS, S.A. como a soma das partes das empresas que possui - The Navigator Company, Secil e ETSA. Esta avaliação segue o formato recomendado pelo CFA Institute. A escolha da Semapa deriva de um interesse neste sector e conhecimento prévio. Este relatório foi realizado através da informação pública disponível até setembro de 2019, e por isso, qualquer informação e evento após esta data não é considerada no trabalho. Para a obtenção do preço-alvo, foram aplicados dois métodos: um absoluto e um relativo. O absoluto foi o Método do Discounted Cash Flow (DCF) e o relativo foi o Método de Múltiplos de Mercado. Na abordagem do DCF foi atingido o preço-alvo de 13.47€ para o fim do ano de 2019, que representa um potencial de valorização de 10.27% em relação ao preço registado pela empresa a 9 setembro de 2019, de 12.22€, sendo a nossa recomendação final para a Semapa de compra. Em relação a abordagem pelo Método de Múltiplos, retornou um preço-alvo de 8.13€, neste caso, inferior ao preço de 9 setembro de 2019 registado pelas ações da Semapa, este fato foi devido às especialidades das subsidiárias (The Navigator Company e Secil) em relação aos seus pares. A nossa avaliação do risco estima ainda um risco baixo para a empresa.This Master's Final Work aims to present a company valuation of Semapa - Sociedade de Investimento e Gestão, SGPS, S.A. as the sum of the parts of the companies it owns - The Navigator Company, Secil and ETSA. This Equity Research follows the format recommended by the CFA Institute. We choose Semapa because it came from an interest in this industry and previous knowledge. This report was made using the public information available at September 2019, and therefore any information and events after this date were not considered. To obtain the target price, two methods were applied: one absolute and one relative. The absolute was the Discounted Cash Flow (DCF) method and the relative was the Market Multiples Approach. In the valuation of DCF was reached the Price Target of EUR 13.47 for the end of the year 2019, that represents an upside potential of 10.27% regarding the price registered by the company on September 9, 2019, of EUR 12.22, our final recommendation for Semapa is to buy. In relation to a Market Multiples Approach, that returned a Price Target of EUR 8.13, in this case, lower than the price on September 9, 2019, recorded by the Semapa shares, this fact was attributed to the specificities of the Semapa, regarding its's peers. Our risk assessment still estimates a low risk for the company.info:eu-repo/semantics/publishedVersio

Curriculum learning for multilevel budgeted combinatorial problems

Learning heuristics for combinatorial optimization problems through graph neural networks have recently shown promising results on some classic NP-hard problems. These are single-level optimization problems with only one player. Multilevel combinatorial optimization problems are their generalization, encompassing situations with multiple players taking decisions sequentially. By framing them in a multi-agent reinforcement learning setting, we devise a value-based method to learn to solve multilevel budgeted combinatorial problems involving two players in a zero-sum game over a graph. Our framework is based on a simple curriculum: if an agent knows how to estimate the value of instances with budgets up to $B$, then solving instances with budget $B+1$ can be done in polynomial time regardless of the direction of the optimization by checking the value of every possible afterstate. Thus, in a bottom-up approach, we generate datasets of heuristically solved instances with increasingly larger budgets to train our agent. We report results close to optimality on graphs up to $100$ nodes and a $185 \times$ speedup on average compared to the quickest exact solver known for the Multilevel Critical Node problem, a max-min-max trilevel problem that has been shown to be at least $\Sigma_2^p$-hard