2,868 research outputs found
Comparison of rectal and tympanic membrane temperature in healthy exercising dogs
The ability to monitor body temperature in athletes at risk of hyperthermia is essential in all species. Currently, the only commonly accepted temperature monitoring site in dogs is the rectum. This is impractical in field situations as it takes time, requires additional handlers to restrain the dog and is not tolerated by all animals. Tympanic membrane temperature (TMT) monitoring may provide a rapid measure of body temperature to facilitate identification of heat stress and heat stroke in canine athletes. In human studies, TMT diverges from rectal temperature (RT) as body temperature increases during exercise induced hyperthermia so is not recommended for monitoring human athletes. If the same divergence occurs in dogs, TMT may not be suitable for use when monitoring the temperature of canine athletes. The aim of the study was to determine if TMT diverged from RT following exercise in healthy dogs. 24 healthy dogs were recruited to the study. Body temperature was measured using a veterinary auricular infrared thermometer (VetTemp) to record tympanic membrane temperature and an electric predictive rectal thermometer. Temperatures were recorded pre and post exercise in a non-clinical setting, familiar to the dogs. The mixed model approach showed that exercise had no effect on the difference between RT and TMT (F(1,201) =0.026, P=0.872). The overall mean difference of RT minus TMT was 0.39°C (n = 116). 68.4% of readings fell within the accepted 0.5°C difference in temperature recording method. In line with previously reported TMT to RT comparison studies in dogs, this study found that TMT measured consistently lower than RT. Using a correction factor of 0.4°C minimised the difference. The hypothesis that dogs would show greater differences between TMT and RT following exercise was not supported, suggesting that TMT could be used to monitor body temperature in exercising dogs where RT is not possible
Field testing two animal-specific non-contact thermometers on healthy horses
This study investigated the accuracy of two animal specific non-contact infrared thermometers (Rycom and Thermofocus) when compared to rectal temperature in healthy horses. One hundred rectal and eye temperatures were measured in 22 horses. Fewer than 50% of the readings taken with the Thermofocus device were within ± 0.5°C of rectal temperature. Over 80% of eye temperature readings taken with the Rycom device were within ± 0.5°C of rectal temperature measured from the medial canthus region. The Rycom thermometer also appears to detect hyperthermia when measuring the left eye temperature; however, clinical patient testing is needed before its use can be recommended
Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression: a Gynaecological Oncology Group Study.
BackgroundLobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human papillomavirus (H-HPV) infection. Carbonic anhydrase-IX (CA-IX) is highly expressed in conventional glandular lesions (CGLs). However, expression of CA-IX in LEGH or GA has not been studied.MethodsIn all, 12 CGLs, 7 LEGHs, 6 LEGHs with coexisting adenocarcinoma in situ (AIS, 3) and GA (3) were identified from Japanese women with a cytological diagnosis of atypical glandular cells of undetermined significance. Immunostaining was used to detect CA-IX and p16(INK)4(a) (hereafter termed p16) protein expression in the tissues and CA-IX protein expression in the Papanicolaou smears (PSs). Polymerase chain reaction was used to detect H-HPV DNA in liquid-based cytology.ResultsOut of 12 (83%) CGLs, 10 were positive with H-HPV and high levels of CA-IX expression were seen in all (100%) cases. P16 protein expression was observed in 11 out of 12 (92%) cases. None of the LEGHs, LEGHs with AIS or GA were positive for H-HPV and only 8 out of 13 (62%) showed focal weak (1+) p16 expression. In contrast, all cases (100%) exhibited strong CA-IX protein expression.ConclusionOur study suggests that there are different molecular mechanisms of carcinogenesis resulting in CGLs vs LEGHs associated with AIS or GA. There is also a possible link between LEGHs and GAs. Furthermore, CA-IX expression may serve as a useful biomarker for the detection of GAs in the absence of H-HPV infection
Drugs, dogs, and driving: the potential for year-round thermal stress in UK vehicles
Background: Dogs are regularly transported or housed in vehicles, with guidelines for housing dogs suggesting that the ambient temperature should be maintained between 15°C and 24°C. Veterinary drugs are routinely stored and carried in vehicles providing ambulatory veterinary care. Non-refrigerated medications typically require storage between 8°C and 25°C.
Aim: This study aims to investigate the potential for thermal stress associated with vehicular storage and transportation of drugs and dogs in a temperate climate, such as the United Kingdom.
Methods: The study used data loggers to continuously record internal temperatures of four vehicles at 15-minute intervals over a two-year period, to investigate the effect of seasonality and time of day on the internal car temperature.
Results: The internal car temperature ranged from −7.4°C to 54.5°C during the study period. Temperatures fell below 8°C every month, except June and July. The internal car temperature exceeded typical drug storage recommendations (>25°C) during every month, and exceeded the canine thermoneutral zone (>35°C) from April to September. Peak temperatures occurred between 14:00 and 17:00 hours.
Conclusion: The results demonstrate the year-round potential for thermal stress of both dogs and drugs left in cars. Public awareness campaigns highlighting the risks of leaving dogs in hot cars are typically launched in late spring, but should consider launching earlier in light of these findings. Veterinary surgeons transporting drugs should take measures to ensure that drugs are stored within the manufacturer’s temperature range year-round. This will limit the potential for drug degradation and decreased efficacy
Investigating factors affecting the body temperature of dogs competing in cross country (canicross) races in the UK
Increasing numbers of people are running with their dogs, particularly in harness through the sport canicross. Whilst canicross races are typically held in the winter months, some human centred events are encouraging running with dogs in summer months, potentially putting dogs at risk of heat related injuries, including heatstroke. The aim of this project was to investigate the effects of ambient conditions and running speed on post-race temperature of canicross dogs in the UK, and investigate the potential risk of heatstroke to canicross racing dogs. The effects of canine characteristics (e.g. gender, coat colour) were explored in order to identify factors that could increase the risk of exercise-induced hyperthermia (defined as body temperature exceeding the upper normal limit of 38.8 °C).108 dogs were recruited from 10 race days, where ambient conditions ranged from -5 to 11 °C measured as universal thermal comfort index (UTCI). 281 post race tympanic membrane temperatures were recorded, ranging from 37.0–42.5 °C. There was a weak correlation between speed and post-race temperature (r = 0.269, P < 0.001). Whilst no correlation between any single environmental factor or UTCI and post-race temperature was found, the proportion of dogs developing exercise-induced hyperthermia during the race increased with UTCI (r = 0.688, P = 0.028). Male dogs (χ(1) = 18.286, P < 0.001), and dark coated dogs (χ(2) = 8.234, P = 0.014), were significantly more likely to finish the race with a temperature exceeding 40.6 °C. Prolonged elevation of body temperature above this temperature is likely to cause heatstroke. At every race dogs exceeded this critical temperature, with 10.7% (n = 30) of the overall study population exceeding this temperature throughout the study period. The results suggest male dogs, dark coloured dogs, and increased speed of running all increase the risk of heatstroke in racing canicross dogs. Further research is required to investigate the impact of environmental conditions on post-race cooling, to better understand safe running conditions for dogs
Establishing a reference range for normal canine tympanic membrane temperature measured with a veterinary aural thermometer
Studies have shown that tympanic membrane temperature (TMT) under reports body temperature when compared to rectal temperature. This could lead to misinterpretation of the TMT, if comparing the result to a rectal temperature range. The aim of this study was to establish a normal canine TMT reference range. 416 TMT’s were taken from 157 healthy dogs, in a range of ambient temperatures. The normal reference range for canine TMT was found to be 36.6-38.8°C. This range should be considered by pet owners and veterinary professionals when interpreting TMT measured with a veterinary aural thermometer, to avoid misinterpretation of the results
Synergism between Medihoney and Rifampicin against Methicillin-Resistant Staphylococcus aureus (MRSA)
Skin and chronic wound infections caused by highly antibiotic resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) are an increasing and urgent health problem worldwide, particularly with sharp increases in obesity and diabetes. New Zealand manuka honey has potent broad-spectrum antimicrobial activity, has been shown to inhibit the growth of MRSA strains, and bacteria resistant to this honey have not been obtainable in the laboratory. Combinational treatment of chronic wounds with manuka honey and common antibiotics may offer a wide range of advantages including synergistic enhancement of the antibacterial activity, reduction of the effective dose of the antibiotic, and reduction of the risk of antibiotic resistance. The aim of this study was to investigate the effect of Medihoney in combination with the widely used antibiotic rifampicin on S. aureus. Using checkerboard microdilution assays, time-kill curve experiments and agar diffusion assays, we show a synergism between Medihoney and rifampicin against MRSA and clinical isolates of S. aureus. Furthermore, the Medihoney/rifampicin combination stopped the appearance of rifampicin-resistant S. aureus in vitro. Methylglyoxal (MGO), believed to be the major antibacterial compound in manuka honey, did not act synergistically with rifampicin and is therefore not the sole factor responsible for the synergistic effect of manuka honey with rifampicin. Our findings support the idea that a combination of honey and antibiotics may be an effective new antimicrobial therapy for chronic wound infections. © 2013 Müller et al
Surveillance of heat-related illness in small animals presenting to veterinary practices in the UK between 2013-2018
Background: Heat-related illness (HRI) can affect all companion animals and is likely to become more common as global temperatures rise. The misconception that HRI is primarily a result of dogs being trapped in hot cars, highlights a lack of awareness of HRI risk factors within the UK companion animal population.
Aim: This project aimed to review all species of small animal presentations of HRI to UK veterinary practices participating in the Small Animal Veterinary Surveillance Network (SAVSNET), describe the inciting triggers and seasonality of HRI events, and review the clinical grade of canine patients presenting with HRI.
Methods: Electronic consultation records were submitted by volunteer veterinary practices across Great Britain to SAVSNET. Cases were defined as animals presented for consultation with strong evidence of current, or recent heat induced illness during the study period (2013-2018).
Results: The HRI cases included 146 dogs, 16 cats, eight guinea pigs, three rabbits and one ferret. Of the 118 HRI cases with a recorded trigger, exercise was the primary trigger for dogs presenting (73.5%); seven (6.9%) canine HRI events followed vehicular confinement. Environmental HRI was recorded as a trigger for the remaining dogs (19.6%), and for all cats, guinea pigs, rabbits and the ferret. Brachycephalic breeds comprised 21.2% of canine HRI cases, and all rabbits were brachycephalic breeds. Dogs presented for HRI between April and October, with 42.5% during July, typically the UK’s hottest month of the year. Cats with HRI were presented between May and September, with 75.0% during June and July. The smaller companion species - ferrets, rabbits and guinea pigs – were presented during the UK’s summer months June to August.
Conclusion: This study highlights the risk of HRI to all pet animals during the UK’s warmer summer months (June to August). The findings support previous claims that exercise is the most common trigger of HRI in dogs, whilst environmental HRI (a hot ambient temperature) accounted for all HRI events in cats, rabbits, guinea pigs and ferrets. Both brachycephalic dogs and rabbits were overrepresented, adding further evidence that owners of these animals should be particularly vigilant for HRI during hot weather
Selection on an antagonistic behavioral trait can drive rapid genital coevolution in the burying beetle, Nicrophorus vespilloides
This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.Male and female genital morphology varies widely across many taxa, and even among populations. Disentangling potential sources of selection on genital morphology is problematic because each sex is predicted to respond to adaptations in the other due to reproductive conflicts of interest. To test how variation in this sexual conflict trait relates to variation in genital morphology we used our previously developed artificial selection lines for high and low repeated mating rates. We selected for high and low repeated mating rates using monogamous pairings to eliminate contemporaneous female choice and male-male competition. Male and female genital shape responded rapidly to selection on repeated mating rate. High and low mating rate lines diverged from control lines after only 10 generations of selection. We also detected significant patterns of male and female genital shape coevolution among selection regimes. We argue that because our selection lines differ in sexual conflict, these results support the hypothesis that sexually antagonistic coevolution can drive the rapid divergence of genital morphology. The greatest divergence in morphology corresponded with lines in which the resolution of intrasexual conflict over mating rate was biased in favor of male interests.Funding was provided by Natural Environment Research Council grants NE/I025468/1 to N.J.R. and A.J.M., and NE/H003738/1 to A.J.M
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