A Survey of Fertility Program Responses of Kentucky Dark Fire-Cured Tobacco (Nicotiana tabacum L.) Yield and Quality for Cigars Manufacture in the Benevento Province (Southern Italy)

Summary Nitrogen (N) fertilization of Kentucky dark fire-cured tobacco can be used to increase weight of high quality cured leaves for cigar manufacture. We conducted field experiments at 11 different locations in the province of Benevento (Southern Italy) where the following four N treatments were compared: 1) unfertilized control (N0); 2) a site-specific N rate, calculated by a N fertilization plan (NFP) based on physical and chemical soil characteristics, which ranged between 113 and 145 kg N ha−1; 3) 200 kg N ha−1 (rate commonly used by farmers, N200); 4) 100 kg N ha−1 (half of the rate commonly used by farmers, N100). Yields of the following five commercial quality categories of cured leaves were measured: i) wrappers, ii) heavy filler (Fh), iii) light filler (Fl), iv) heavy shredded (Sh) and v) light shredded (Sl). Fh cured products of B1, B4, B6 and B10 locations were analyzed for: total alkaloids, reducing sugars, chlorides, total N (Kjeldahl), ammonium-N (NH4-N), nitrate-N (NO3-N), and tobacco specific nitrosamines (TSNA). Color parameters: Lightness (L), Chroma (C) and Hue (H) were determined on five cured leaves / plot of both Fh and Fl types at B1, B2, B3, B6, B8 and B10. A blind evaluation of cured leaves collected across locations was conducted by a panel test who considered the main basic characteristics of cured leaves (stalk position, leaf structure, texture, etc.). The total yield of cured products increased with fertilization across locations, up to NFP treatment, without any statistically significant increase at N200 treatment. Fertilization increased yield of wrappers at B1 up to NFP treatment (113.5 kg N ha−1), without any significant increase at N200 treatment. Yield of light filler product was positively influenced by fertilization up to the maximum dose only in 5 out of 11 locations. Total alkaloids significantly increased with increasing fertilization up to 100 kg N ha−1 without any significant changes at higher N rate. Fertilization hardly increased L and C of Fl leaves, which appeared light-brown but brilliant-colored. There was a reduction in the H value of Fh products which indicated a darker tone of those leaves with respect to Fl. Increasing N rate affected production costs more than revenues. According to agronomic results, in most locations the best results in terms of net revenues were obtained by NFP treatment. Considering that N rates above the NFP would disqualify growers from the economic aids provided by EU agrienvironmental measures, by economic point of view the NFP treatment would be recommended over the current grower standard (N200)

Compost and microbial biostimulant applications improve plant growth and soil biological fertility of a grass-based phytostabilization system

In this work, a grass-based phytoremediation system integrated with an organic amendment and biostimulants was evaluated for remediating contaminated sites. Plant growth and biological fertility were monitored to assess the efficacy of a vegetative cap used as a safety measure to reduce sanitary and environmental risks of industrially contaminated soils and soil-washing sludges. Both matrices were potentially contaminated with Pb and Zn with an ecological risk index from low to moderate. According to potentially toxic elements (PTEs) bioaccessibility tests, the exposure to the released fine particulate matter may cause serious risks to human beings, in particular to children. The grass mixture was well adapted to both the substrates and a low PTEs mobility was detected, thus, reducing the leaching risk to ground water sources. Compost addition augmented significantly nitrogenase reductase (nifH) and ammonia monooxygenase (amoA) gene expression abundance in both substrates. Furthermore, a positive interaction between compost fertilization and a Trichoderma-based biostimulant inoculation was recorded in sludges resulting in a significant stimulation of nitrogen-fixing and ammonia-oxidizing bacteria. The application of compost and biostimulant increased soil fertility and plant growth. Furthermore, there was a slight reduction in PTE bioaccessibility, thus, improving the efficiency of the phytostabilization, limiting the resuspension and dispersion of the health-risk soil particulate

Reliability of MRI findings in candidates for lumbar disc prosthesis

Introduction: Limited reliability data exist for localised magnetic resonance imaging (MRI) findings relevant to planning of treatment with lumbar disc prosthesis and later outcomes. We assessed the reliability of such findings in chronic low back pain patients who were accepted candidates for disc prosthesis. Methods: On pretreatment MRI of 170 patients (mean age 41 years; 88 women), three experienced radiologists independently rated Modic changes, disc findings and facet arthropathy at L3/L4, L4/L5 and L5/S1. Two radiologists rerated 126 examinations. For each MRI finding at each disc level, agreement was analysed using the kappa statistic and differences in prevalence across observers using a fixed effects model. Results: All findings at L3/L4 and facet arthropathy at L5/S1 had a mean prevalence <10% across observers and were not further analysed, ensuring interpretable kappa values. Overall interobserver agreement was generally moderate or good (kappa 0.40–0.77) at L4–S1 for Modic changes, nucleus pulposus signal, disc height (subjective and measured), posterior high-intensity zone (HIZ) and disc contour, and fair (kappa 0.24) at L4/L5 for facet arthropathy. Posterior HIZ at L5/S1 and severely reduced subjective disc height at L4/L5 differed up to threefold in prevalence between observers (p< 0.0001). Intraobserver agreement was mostly good or very good (kappa 0.60–1.00). Conclusion: In candidates for disc prosthesis, mostly moderate interobserver agreement is expected for localised MRI findings

Phytomanagement of contaminated and marginal lands with Ricinus communis L.

Soils are a non-renewable resource increasingly endangered by human activities at an unsustainable rate. Suffice it to say that the extent of all forms of soil degradation is estimated at 10 million hectares per year. Biophysical degradation of soils caused by land misuse and/or overuse and its consequential economic effects lead to the generation of the so-called marginal lands. According to global demographic estimates, there will be a minimum of nine billion people worldwide by the year 2050, leading to an increase in the demand for food and energy production. As follows, this entails that marginal soils must be recovered, reclaimed, and utilized in the best possible way while restoring ecosystem services. However, marginal areas' biophysical limitations might reduce crop yield, being frequently tackled by low fertility often coupled with a shortage of freshwater supplies, and contaminations with potentially toxic elements (PTEs) that can limit crop growth. With this in mind, the present work looks at the phytomanagement of marginal areas using the multipurpose crop Ricinus communis L. and analyzes different techniques that can be employed to increase its production and remediation efficacy. In order to accomplish these claims, the trials here reported were carried out testing a variety of organic amendments (waste by-products, i.e., compost obtained from the solid fraction of urban waste, anaerobically digested municipal sewage sludge, biochar produced from lignocellulosic biomasses employed for phytoremediation purposes) and remediation methods at different scales, such as field, greenhouse, growth chamber, and laboratory. Ricinus communis L. was tested for its tolerance to extremely low fertility and saline conditions, as well as soil contamination (specifically soil contaminated with Pb, As, and Sb due to shooting activities), and qualified for its suitability for phytomanagement of marginal lands, through the measurement of several physiological and biochemical indices. The results obtained emphasize the viability of using Ricinus communis L. for the phytomanagement of marginal lands. When grown on extremely poor substrates, some cultivars of castor bean were able to germinate, grow and produce oil seeds and residual biomass, even under saline irrigation. As an example, the germination of one of the tested cultivars was enhanced by saline irrigation at 4 dS m-1 (+ 3.3% more than the control at 0 dS m-1). Nevertheless, castor bean production was increased when supported by a combination of compost and biochar as organic amendments (up to 5.4-fold, compared to the unamended control). This demonstrates how by-products of organic waste can be used to restore marginal land while improving the growth of bioenergy crops, enhancing carbon sequestration, and disposing of organic waste in an environmentally responsible manner. Moreover, the use of organic amendments proved the immobilization of some PTEs and the mobilization of others. As an example, the reduction of the exchangeable soil fraction of Sb was achieved with the application of digestate produced from the anaerobic digestion of municipal sewage sludge, while biochar application enhanced its bioavailability. The application of arbuscular mycorrhiza fungi limited the accumulation of Pb, As, and Sb in Ricinus plants (on average -14% in leaves, stem and roots for all the PTEs). The findings of this work contribute to the possibilities of the eco-restoration of degraded, contaminated and marginal lands, through the adaptative capacities of Ricinus communis L

A Survey of Fertility Program Responses of Kentucky Dark Fire-Cured Tobacco (Nicotiana tabacum L.) Yield and Quality for Cigars Manufacture in the Benevento Province (Southern Italy)

Nitrogen (N) fertilization of Kentucky dark fire-cured tobacco can be used to increase weight of high quality cured leaves for cigar manufacture. We conducted field experiments at 11 different locations in the province of Benevento (Southern Italy) where the following four N treatments were compared: 1) unfertilized control (N0); 2) a site-specific N rate, calculated by a N fertilization plan (NFP) based on physical and chemical soil characteristics, which ranged between 113 and 145 kg N ha−1; 3) 200 kg N ha−1 (rate commonly used by farmers, N200); 4) 100 kg N ha−1 (half of the rate commonly used by farmers, N100). Yields of the following five commercial quality categories of cured leaves were measured: i) wrappers, ii) heavy filler (Fh), iii) light filler (Fl), iv) heavy shredded (Sh) and v) light shredded (Sl). Fh cured products of B1, B4, B6 and B10 locations were analyzed for: total alkaloids, reducing sugars, chlorides, total N (Kjeldahl), ammonium-N (NH4-N), nitrate-N (NO3-N), and tobacco specific nitrosamines (TSNA). Color parameters: Lightness (L), Chroma (C) and Hue (H) were determined on five cured leaves / plot of both Fh and Fl types at B1, B2, B3, B6, B8 and B10. A blind evaluation of cured leaves collected across locations was conducted by a panel test who considered the main basic characteristics of cured leaves (stalk position, leaf structure, texture, etc.). The total yield of cured products increased with fertilization across locations, up to NFP treatment, without any statistically significant increase at N200 treatment. Fertilization increased yield of wrappers at B1 up to NFP treatment (113.5 kg N ha−1), without any significant increase at N200 treatment. Yield of light filler product was positively influenced by fertilization up to the maximum dose only in 5 out of 11 locations. Total alkaloids significantly increased with increasing fertilization up to 100 kg N ha−1 without any significant changes at higher N rate. Fertilization hardly increased L and C of Fl leaves, which appeared light-brown but brilliant-colored. There was a reduction in the H value of Fh products which indicated a darker tone of those leaves with respect to Fl. Increasing N rate affected production costs more than revenues. According to agronomic results, in most locations the best results in terms of net revenues were obtained by NFP treatment. Considering that N rates above the NFP would disqualify growers from the economic aids provided by EU agrienvironmental measures, by economic point of view the NFP treatment would be recommended over the current grower standard (N200)

Decomposition and organic amendments chemistry explain contrasting effects on plant growth promotion and suppression of Rhizoctonia solani damping off.

Organic Amendments (OAs) has been used in agroecosystems to promote plant growth and control diseases caused by soilborne pathogens. However, the role of OAs chemistry and decomposition time on plant growth promotion and disease suppression is still poorly explored. In this work, we studied the effect of 14 OAs at four decomposition ages (3, 30, 100, and 300 days) on the plant-pathogen system Lactuca sativa-Rhizoctonia solani. OAs chemistry was characterized via 13C-CPMAS NMR spectroscopy as well as for standard chemical (i.e. N content, pH, EC) and biological parameters (i.e. phytotoxicity and R. solani proliferation bioassay). OAs have shown variable effects, ranging from inhibition to stimulation of Lactuca sativa and Lepidium sativum growth. We recorded that N rich OAs with high decomposability were conducive in the short-term, while converting suppressive in the long term (300 days). On the other hand, cellulose-rich OAs with high C/N ratio impaired L. sativa growth but were more consistent in providing protection from damping-off, although this property has significantly shifted during decomposition time. These results, for the first time, highlight a consistent trade-off between plant growth promotion and disease control capability of OAs. Finally, we found that OAs effects on growth promotion and disease protection can be hardly predictable based on the chemical characteristic, although N content and some 13C CPMAS NMR regions (alkyl C, methoxyl C, and carbonyl C) showed some significant correlations. Therefore, further investigations are needed to identify the mechanism(s) behind the observed suppressive and conducive effects and to identify OAs types and application timing that optimize plant productivity and disease suppression in different agro-ecosystems

Surgeons’ practice and preferences for the anal fissure treatment: results from an international survey

The best nonoperative or operative anal fissure (AF) treatment is not yet established, and several options have been proposed. Aim is to report the surgeons' practice for the AF treatment. Thirty-four multiple-choice questions were developed. Seven questions were about to participants' demographics and, 27 questions about their clinical practice. Based on the specialty (general surgeon and colorectal surgeon), obtained data were divided and compared between two groups. Five-hundred surgeons were included (321 general and 179 colorectal surgeons). For both groups, duration of symptoms for at least 6 weeks is the most important factor for AF diagnosis (30.6%). Type of AF (acute vs chronic) is the most important factor which guide the therapeutic plan (44.4%). The first treatment of choice for acute AF is ointment application for both groups (59.6%). For the treatment of chronic AF, this data is confirmed by colorectal surgeons (57%), but not by the general surgeons who prefer the lateral internal sphincterotomy (LIS) (31.8%) (p = 0.0001). Botulin toxin injection is most performed by colorectal surgeons (58.7%) in comparison to general surgeons (20.9%) (p = 0.0001). Anal flap is mostly performed by colorectal surgeons (37.4%) in comparison to general surgeons (28.3%) (p = 0.0001). Fissurectomy alone is statistically significantly most performed by general surgeons in comparison to colorectal surgeons (57.9% and 43.6%, respectively) (p = 0.0020). This analysis provides useful information about the clinical practice for the management of a debated topic such as AF treatment. Shared guidelines and consensus especially focused on operative management are required to standardize the treatment and to improve postoperative results