51 research outputs found
NARRATIVAS ORAIS CONTRIBUEM PARA O DESENVOLVIMENTO LINGUÍSTICO DE CRIANÇAS COM CEGUEIRA?
O artigo propõe um Programa (PRONARRO) de estímulo à criação de narrativas orais de criança com cegueira como ferramenta para minimizar a defasagem linguística que existe entre a criança cega e a que vê e apresenta condutas para preparação do profissional e da família dessas crianças. Os procedimentos empregados para a aplicação das etapas do referido Programasão desenvolvidos através da “eliciação” (perguntas dirigidas à criança) ocorrida durante as sessões, quando o adulto interlocutor dirige perguntas à criança tentando levá-la a produzir narrativas. Estas orientações obedecem ao modelo laboviano de narrativas que estabelecem a necessidade da dependência temporal entre enunciados e o uso de verbos de ação. Ressalta a linguagem como principal elemento para a aprendizagem no processo de socialização entre a pessoa que vê e aquela que não vê
BASES PARA A APRENDIZAGEM DE LÍNGUAS ESTRANGEIRAS
Este trabalho evidencia uma reflexão sobre as bases deaprendizagem da língua estrangeira, a partir da nossa experiência comoprofessoras de prática de ensino. Destacamos ser a motivação um dospilares para o avanço em qualquer situação de aprendizagem, o que temsido deixado de lado em instâncias como a escola pública. Um outropilar, ao qual nos referimos, com bastante rigor, é o professor competentecomo a melhor base de sustentação na efetiva aprendizagem
Kinetics of optical properties of colorectal muscle during optical clearing
In this paper, we describe a simple and indirect method to evaluate the kinetics of the optical properties for biological tissues under optical clearing treatments. We use the theoretical formalism in this method to process experimental data obtained from colorectal muscle samples to evaluate and characterize the dehydration and refractive index matching mechanisms
Kinetics of optical properties of human colorectal tissues during optical clearing: a comparative study between normal and pathological tissues
To characterize the optical clearing treatments in human colorectal tissues and possibly to differentiate
between treatments of normal and pathological tissues, we have used a simple indirect method derived
from Mie scattering theory to estimate the kinetics of the reduced scattering coefficient. A complementary
method to estimate the kinetics of the scattering coefficient is also used so that the kinetics of the anisotropy
factor and of the refractive index are also calculated. Both methods rely only on the thickness and collimated
transmittance measurements made during treatment. The results indicate the expected time dependencies
for the optical properties of both tissues: an increase in the refractive index and anisotropy factor and a decrease in the scattering coefficients. The similarity in the kinetics obtained for normal and pathological tissues indicates that optical clearing treatments can be applied also in pathological tissues to produce similar effects. The estimated time dependencies using experimental spectral data in the range from 400 to 1000 nm allowed us to compare the kinetics of the optical properties between different wavelengths.info:eu-repo/semantics/publishedVersio
Como lidar com o bullying e preveni-lo : uma proposta de ação no contexto da Unidade Letiva 1 do 5º ano do programa de Educação Moral Religiosa Católica : Viver juntos
Como professora de EMRC apercebo-me que existe uma grande mudança nas crianças que
passam do 1º Ciclo para o 2º Ciclo. Esta transição desencadeia, por vezes, comportamentos
desadequados que podem promover o desenvolvimento de Bullying. Sendo este um tema
que abordo regularmente nas aulas de EMRC, gostaria que o mesmo fosse trabalhado no
contexto da Unidade Letiva 1 – Viver juntos. Com este exercício pretendo sublinhar o
contributo que a EMRC pode dar na abordagem desta realidade, para a qual temos de estar
diariamente atentos.As an EMRC teacher I realize that there is a big change in the children who go from 1st Cycle
to 2nd Cycle. This transition sometimes triggers inappropriate behaviors that can promote
the development of bullying. As this is a theme I regularly work in EMRC classes, I would like
it to be worked on in the context of Teaching Unit 1 - Living Together. With this exercise I
want to underline the contribution that EMRC can make in addressing this reality, to which
we must be aware daily
Diffuse reflectance and machine learning techniques to differentiate colorectal cancer ex vivo
In this study, we used machine learning techniques to reconstruct the wavelength dependence of the absorption coefficient of human
normal and pathological colorectal mucosa tissues. Using only diffuse reflectance spectra from the ex vivo mucosa tissues as input to
algorithms, several approaches were tried before obtaining good matching between the generated absorption coefficients and the ones
previously calculated for the mucosa tissues from invasive experimental spectral measurements. Considering the optimized match for the
results generated with the multilayer perceptron regression method, we were able to identify differentiated accumulation of lipofuscin in
the absorption coefficient spectra of both mucosa tissues as we have done before with the corresponding results calculated directly from
invasive measurements. Considering the random forest regressor algorithm, the estimated absorption coefficient spectra almost matched
the ones previously calculated. By subtracting the absorption of lipofuscin from these spectra, we obtained similar hemoglobin ratios at
410/550 nm: 18.9-fold/9.3-fold for the healthy mucosa and 46.6-fold/24.2-fold for the pathological mucosa, while from direct calculations,
those ratios were 19.7-fold/10.1-fold for the healthy mucosa and 33.1-fold/17.3-fold for the pathological mucosa. The higher values obtained
in this study indicate a higher blood content in the pathological samples used to measure the diffuse reflectance spectra. In light of such
accuracy and sensibility to the presence of hidden absorbers, with a different accumulation between healthy and pathological tissues, good
perspectives become available to develop minimally invasive spectroscopy methods for in vivo early detection and monitoring of colorectal
cancer.The application of machine learning methods to noninvasivelike diffuse reflectance spectra allowed us to reconstruct the
absorption coefficient spectra of human healthy and pathological
mucosa tissues from the colorectal wall. Consequently, we were
able to obtain differentiated blood and pigment content in both
tissues, which can be used for the development of new noninvasive
diagnostic methods for colorectal cancer.The work of L. M. Oliveira was supported by the Portuguese Science Foundation (Grant No. FCT-UIDB/04730/2020). The work of V. V. Tuchin was supported by a grant of the Government of the Russian Federation (Registration No. 2020-220-08-2389).info:eu-repo/semantics/publishedVersio
Tissue Spectroscopy and Optical Clearing of Colorectal Mucosa in the Pursuit of New Cancer Diagnostic Approaches
In this paper we present three studies that demonstrate the applicability of spectroscopy methods and optical clearing treatments in pathology identification and monitoring. In the first study, by obtaining the absorption spectra of human healthy and pathological (adenocarcinoma) colorectal mucosa tissues, it was possible to identify a higher content of a pigment in the diseased tissues. This study also shows that machine learning methods can be used to reach the same differentiated results in vivo through diffuse reflectance spectroscopy. In the second study, the combination of collimated transmittance spectroscopy with optical clearing treatments allowed to obtain the diffusion coefficients of glucose in healthy and pathological colorectal mucosa as: Dglucose=5.8x10–7 cm2/s and Dglucose=4.4x10–7 cm2/s, respectively. This study also demonstrated that the diseased tissues contains about 5% more mobile water than the healthy tissues. The third study was performed to evaluate the protein dissociation mechanism of optical clearing. By treating both healthy and pathological colorectal mucosa tissues with 93%-glycerol, a protein dissociation rate of about 3 times higher was obtained for the pathological mucosa. All the discriminating parameters that result from these studies can be obtained in the in vivo situation through diffuse reflectance spectroscopy and further studies to evaluate their values in different stages of cancer progression are of great importance to develop disease monitoring protocols.The authors of the article knew well and communicated
with Ekaterina Borisova for many years, especially
Valery Tuchin and in recent years Luís Oliveira. We had
many joint plans to organize conferences and joint
research projects, but COVID-19 mercilessly interrupted
our communication. We have lost a great scientist and a
person with a huge soul, sociable, but at the same time
modest and kind. We will always remember our warm
meetings and fruitful work with Ekaterina.
This research was supported by the Portuguese grant
FCT-UIDB/04730/2020.
VVT was supported by grant under the Decree of the
Government of the Russian Federation No. 220 of 09
April 2010 (Agreement No. 075-15-2021-615 of 04 June
2021).info:eu-repo/semantics/publishedVersio
Fast calculation of spectral optical properties and pigment content detection in human normal and pathological kidney
A fast calculation method was used to obtain the spectral optical properties of human normal and pathological (chromophobe renal cell carcinoma) kidney tissues. Using total transmittance, total reflectance and collimated transmittance spectra acquired from ex vivo kidney samples, the spectral optical properties of both tissues, namely the absorption, the scattering and the reduced scattering coefficients, as well as the scattering anisotropy, dispersion and light penetration depth, were calculated between 200 and 1000 nm. Analysis of the mean ab sorption coefficient spectra of the kidney tissues showed that both contain melanin and lipofuscin, and that 83 % of the melanin in the normal kidney converts into lipofuscin in the pathological kidney.info:eu-repo/semantics/publishedVersio
A Multiplex Test Assessing MiR663ame and VIMme in Urine Accurately Discriminates Bladder Cancer from Inflammatory Conditions
Bladder cancer (BlCa) is a common malignancy with significant morbidity and mortality. Current diagnostic methods are invasive and costly, showing the need for newer biomarkers. Although several epigenetic-based biomarkers have been proposed, their ability to discriminate BlCa from common benign conditions of the urinary tract, especially inflammatory diseases, has not been adequately explored. Herein, we sought to determine whether VIMme and miR663ame might accurately discriminate those two conditions, using a multiplex test. Performance of VIMme and miR663ame in tissue samples and urines in testing set confirmed previous results (96.3% sensitivity, 88.2% specificity, area under de curve (AUC) 0.98 and 92.6% sensitivity, 75% specificity, AUC 0.83, respectively). In the validation sets, VIMme-miR663ame multiplex test in urine discriminated BlCa patients from healthy donors or patients with inflammatory conditions, with 87% sensitivity, 86% specificity and 80% sensitivity, 75% specificity, respectively. Furthermore, positive likelihood ratio (LR) of 2.41 and negative LR of 0.21 were also disclosed. Compared to urinary cytology, VIMme-miR663ame multiplex panel correctly detected 87% of the analysed cases, whereas cytology only forecasted 41%. Furthermore, high miR663ame independently predicted worse clinical outcome, especially in patients with invasive BlCa. We concluded that the implementation of this panel might better stratify patients for confirmatory, invasive examinations, ultimately improving the cost-effectiveness of BlCa diagnosis and management. Moreover, miR663ame analysis might provide relevant information for patient monitoring, identifying patients at higher risk for cancer progression
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