Pandemic (H1N1) 2009 outbreak on pig farm, Argentina

In June-July 2009, an outbreak of pandemic (H1N1) 2009 infection occurred on a pig farm in Argentina. Molecular analysis indicated that the virus was genetically related to the pandemic (H1N1) 2009 influenza virus strain. The outbreak presumably resulted from direct human-to-pig transmission.Facultad de Ciencias Veterinaria

Pandemic (H1N1) 2009 Outbreak on Pig Farm, Argentina

In June–July 2009, an outbreak of pandemic (H1N1) 2009 infection occurred on a pig farm in Argentina. Molecular analysis indicated that the virus was genetically related to the pandemic (H1N1) 2009 influenza virus strain. The outbreak presumably resulted from direct human-to-pig transmission

Viral determination and molecular characterization of Aujeszky’s disease virus in slaughter plants and wild boar, as part as the epidemiological surveillance official program in Argentina

La enfermedad de Aujeszky afecta principalmente a la producción porcina y ocasiona pérdidas económicas importantes. En la Argentina, esta enfermedad es considerada endémica, lo que puede resultar en restricciones de comercialización para la región. Las exigencias del mercado externo obligan a avanzar con los planes de control y erradicación de la enfermedad de Aujezsky. Esta enfermedad limita las posibilidades económicas del sector porcino y la comercialización internacional, lo cual influye negativamente en la rentabilidad de las explotaciones y en la calidad de los productos de origen animal. Las técnicas serológicas utilizadas solo detectan la presencia de anticuerpos específicos y no permiten detectar directamente la presencia del patógeno en el organismo ni una infección latente. La utilización de la biología molecular para la confirmación y diagnóstico de la enfermedad de Aujeszky en los planes de vigilancia puede convertirse en una herramienta eficaz para la detección temprana de animales infectados con el virus, así como también para la identificación de las variantes genotípicas que circulan en el país. Este conocimiento aportaría información para establecer políticas sanitarias enfocadas en el control de la enfermedad de Aujeszky, tendientes a la erradicación del virus Herpesvirus porcino tipo 1 (SuHV-1) circulante en la Argentina.Aujezky´s disease principally affects the porcine production, and it generate important economical lost. Currently, In Argentina, Aujeszky is consider an endemic disease and could result in regional commercial restrictions. International requirements for commercialization oblates to develop plans for control and eradication of Aujezsky´s disease. Serological techniques currently used do not allow to identify if the animal is free of pathogen or if it could be a healthy carrier. In surveillance programs, the implementation of Molecular techniques to confirm the diagnostic of this disease is a necessary tool for obtaining early and rapid results. Furthermore, it is also necessary to achieve the molecular characterization of the circulating strains in Argentina in order to establish sanitary politics for eradication of Aujeszky´s disease. This information could allow establishing sanitary politics based on the control of Aujeszky’s diseases and eradication of the circulating Suid Herpesvirus type 1 (SuHV-1).Facultad de Ciencias Veterinaria

Viral determination and molecular characterization of Aujeszky’s disease virus in slaughter plants and wild boar, as part as the epidemiological surveillance official program in Argentina

La enfermedad de Aujeszky afecta principalmente a la producción porcina y ocasiona pérdidas económicas importantes. En la Argentina, esta enfermedad es considerada endémica, lo que puede resultar en restricciones de comercialización para la región. Las exigencias del mercado externo obligan a avanzar con los planes de control y erradicación de la enfermedad de Aujezsky. Esta enfermedad limita las posibilidades económicas del sector porcino y la comercialización internacional, lo cual influye negativamente en la rentabilidad de las explotaciones y en la calidad de los productos de origen animal. Las técnicas serológicas utilizadas solo detectan la presencia de anticuerpos específicos y no permiten detectar directamente la presencia del patógeno en el organismo ni una infección latente. La utilización de la biología molecular para la confirmación y diagnóstico de la enfermedad de Aujeszky en los planes de vigilancia puede convertirse en una herramienta eficaz para la detección temprana de animales infectados con el virus, así como también para la identificación de las variantes genotípicas que circulan en el país. Este conocimiento aportaría información para establecer políticas sanitarias enfocadas en el control de la enfermedad de Aujeszky, tendientes a la erradicación del virus Herpesvirus porcino tipo 1 (SuHV-1) circulante en la Argentina.Aujezky´s disease principally affects the porcine production, and it generate important economical lost. Currently, In Argentina, Aujeszky is consider an endemic disease and could result in regional commercial restrictions. International requirements for commercialization oblates to develop plans for control and eradication of Aujezsky´s disease. Serological techniques currently used do not allow to identify if the animal is free of pathogen or if it could be a healthy carrier. In surveillance programs, the implementation of Molecular techniques to confirm the diagnostic of this disease is a necessary tool for obtaining early and rapid results. Furthermore, it is also necessary to achieve the molecular characterization of the circulating strains in Argentina in order to establish sanitary politics for eradication of Aujeszky´s disease. This information could allow establishing sanitary politics based on the control of Aujeszky’s diseases and eradication of the circulating Suid Herpesvirus type 1 (SuHV-1).Facultad de Ciencias Veterinaria

Viral determination and molecular characterization of Aujeszky’s disease virus in slaughter plants and wild boar, as part as the epidemiological surveillance official program in Argentina

La enfermedad de Aujeszky afecta principalmente a la producción porcina y ocasiona pérdidas económicas importantes. En la Argentina, esta enfermedad es considerada endémica, lo que puede resultar en restricciones de comercialización para la región. Las exigencias del mercado externo obligan a avanzar con los planes de control y erradicación de la enfermedad de Aujezsky. Esta enfermedad limita las posibilidades económicas del sector porcino y la comercialización internacional, lo cual influye negativamente en la rentabilidad de las explotaciones y en la calidad de los productos de origen animal. Las técnicas serológicas utilizadas solo detectan la presencia de anticuerpos específicos y no permiten detectar directamente la presencia del patógeno en el organismo ni una infección latente. La utilización de la biología molecular para la confirmación y diagnóstico de la enfermedad de Aujeszky en los planes de vigilancia puede convertirse en una herramienta eficaz para la detección temprana de animales infectados con el virus, así como también para la identificación de las variantes genotípicas que circulan en el país. Este conocimiento aportaría información para establecer políticas sanitarias enfocadas en el control de la enfermedad de Aujeszky, tendientes a la erradicación del virus Herpesvirus porcino tipo 1 (SuHV-1) circulante en la Argentina.Aujezky´s disease principally affects the porcine production, and it generate important economical lost. Currently, In Argentina, Aujeszky is consider an endemic disease and could result in regional commercial restrictions. International requirements for commercialization oblates to develop plans for control and eradication of Aujezsky´s disease. Serological techniques currently used do not allow to identify if the animal is free of pathogen or if it could be a healthy carrier. In surveillance programs, the implementation of Molecular techniques to confirm the diagnostic of this disease is a necessary tool for obtaining early and rapid results. Furthermore, it is also necessary to achieve the molecular characterization of the circulating strains in Argentina in order to establish sanitary politics for eradication of Aujeszky´s disease. This information could allow establishing sanitary politics based on the control of Aujeszky’s diseases and eradication of the circulating Suid Herpesvirus type 1 (SuHV-1).Facultad de Ciencias Veterinaria

Pandemic (H1N1) 2009 outbreak on pig farm, Argentina

In June-July 2009, an outbreak of pandemic (H1N1) 2009 infection occurred on a pig farm in Argentina. Molecular analysis indicated that the virus was genetically related to the pandemic (H1N1) 2009 influenza virus strain. The outbreak presumably resulted from direct human-to-pig transmission.Facultad de Ciencias Veterinaria

Light-based vat-polymerization bioprinting

Light-based vat-polymerization bioprinting enables computer-aided patterning of 3D cell-laden structures in a point-by-point, layer-by-layer or volumetric manner, using vat (vats) filled with photoactivatable bioresin (bioresins). This collection of technologies — divided by their modes of operation into stereolithography, digital light processing and volumetric additive manufacturing — has been extensively developed over the past few decades, leading to broad applications in biomedicine. In this Primer, we illustrate the methodology of light-based vat-polymerization 3D bioprinting from the perspectives of hardware, software and bioresin selections. We follow with discussions on methodological variations of these technologies, including their latest advancements, as well as elaborating on key assessments utilized towards ensuring qualities of the bioprinting procedures and products. We conclude by providing insights into future directions of light-based vat-polymerization methods

Spontaneous non-canonical assembly of CcmK hexameric components from β-carboxysome shells of cyanobacteria

CcmK proteins are major constituents of icosahedral shells of beta-carboxysomes, a bacterial microcompartment that plays a key role for CO2 fixation in nature. Supported by the characterization of bidimensional (2D) layers of packed CcmK hexamers in crystal and electron microscopy structures, CcmK are assumed to be the major components of icosahedral flat facets. Here, we reassessed the validity of this model by studying CcmK isoforms from Synechocystis sp. PCC6803. Native mass spectrometry studies confirmed that CcmK are hexamers in solution. Interestingly, potential pre-assembled intermediates were also detected with CcmK2. Atomic-force microscopy (AFM) imaging under quasi-physiological conditions confirmed the formation of canonical flat sheets with CcmK4. Conversely, CcmK2 formed both canonical and striped-patterned patches, while CcmK1 assembled into remarkable supra-hexameric curved honeycomb-like mosaics. Mutational studies ascribed the propensity of CcmK1 to form round assemblies to a combination of two features shared by at least one CcmK isoform in most beta-cyanobacteria: a displacement of an alpha helical portion towards the hexamer edge, where a potential phosphate binding funnel forms between packed hexamers, and the presence of a short C-terminal extension in CcmK1. All-atom molecular dynamics supported a contribution of phosphate molecules sandwiched between hexamers to bend CcmK1 assemblies. Formation of supra-hexameric curved structures could be reproduced in coarse-grained simulations, provided that adhesion forces to the support were weak. Apart from uncovering unprecedented CcmK self-assembly features, our data suggest the possibility that transitions between curved and flat assemblies, following cargo maturation, could be important for the biogenesis of beta-carboxysomes, possibly also of other BMC

Correction to: Light-based vat-polymerization bioprinting (Nature Reviews Methods Primers, (2023), 3, 1, (47), 10.1038/s43586-023-00231-0)

Correction to: Nature Reviews Methods Primershttps://doi.org/10.1038/s43586-023-00231-0, published online 22 June 2023 In the version of the article initially published, the orientation of the dose test panels in Fig. 3c was incorrect. The panels have now been amended in the HTML and PDF versions of the article

Light-based vat-polymerization bioprinting

Light-based vat-polymerization bioprinting enables computer-aided patterning of 3D cell-laden structures in a point-by-point, layer-by-layer or volumetric manner, using vat (vats) filled with photoactivatable bioresin (bioresins). This collection of technologies — divided by their modes of operation into stereolithography, digital light processing and volumetric additive manufacturing — has been extensively developed over the past few decades, leading to broad applications in biomedicine. In this Primer, we illustrate the methodology of light-based vat-polymerization 3D bioprinting from the perspectives of hardware, software and bioresin selections. We follow with discussions on methodological variations of these technologies, including their latest advancements, as well as elaborating on key assessments utilized towards ensuring qualities of the bioprinting procedures and products. We conclude by providing insights into future directions of light-based vat-polymerization methods.ISSN:2662-844