The mesolithic occupation at Isolidda (San Vito Lo Capo), Sicily

‘Gruppo dell’Isolidda’ is a complex of five caves along a rocky cliff on the eastern side of the promontory of San Vito Lo Capo (Trapani) in NW Sicily. In 2004 archaeological excavations in the slope below the caves revealed a stratified deposit, partially in secondary position, containing levels with Late Epigravettian and Mesolithic stone tool assemblages. Early Mesolithic stone tool industries, characterized by backed microlithic tools, were distributed in two contiguous layers (SU 21 and SU 25), the lowest of which (SU 21) also contained Epigravettian tools, probably due to sediment reworking. Three AMS dates on Phorcus turbinatus shells (~9520-8990 cal. BP) are chronologically compatible with the Early Mesolithic materials and suggest that the bulk of the deposit accumulated then. A third level, lying above the previous ones, contained material culture associated to the Late Mesolithic or Early Neolithic. Faunal remains from the site represent mainly food refuse and included abundant shells of intertidal molluscs (e.g. Phorcus turbinatus and Patella sp.), along with few fragmented bones of terrestrial herbivores (e.g. Cervus elaphus and Sus scrofa). Oxygen isotope analyses on shell carbonates of Phorcus turbinatus show that, around 9520-9000 cal. BP, marine molluscs were exploited year-round, albeit more often in autumn and winter

180 years of marine animal diversity as perceived by public media in southern Brazil

Unidad de excelencia María de Maeztu CEX2019-000940-MCommoditization of marine resources has dramatically increased anthropogenic footprints on coastal and ocean systems, but the scale of these impacts remain unclear due to a pervasive lack of historical baselines. Through the analysis of historical newspapers, this paper explores changes in marine animals (vertebrates and invertebrates) targeted by historical fisheries in southern Brazil since the late 19th century. The investigation of historical newspaper archives revealed unprecedented information on catch composition, and perceived social and economic importance of key species over decades, predating official national-level landing records. We show that several economically and culturally important species have been under persistent fishing pressure at least since the first national-scale subsidies were introduced for commercial fisheries in Brazil in the late 19th and early 20th centuries. Our work expands the current knowledge on historical fish catch compositions in the southwestern Atlantic Ocean, while advocating for the integration of historical data in ocean sustainability initiatives

Elemental mapping of Mg/Ca intensity ratios in marine mollusc shells using laser-induced breakdown spectroscopy

Records of past environmental conditions in shell carbonate are usually derived from compositional analysis (i.e. trace elements, stable oxygen, carbon, and nitrogen isotopes) performed along the direction of the shell’s growth and thus through time. However, compositional variations within isochronous parts of the shell can distort the environmental record and are difficult to assess without extensively mapping the whole shell. Here we apply Laser Induced Breakdown Spectroscopy (LIBS) to efficiently map the elemental change throughout the growth increments of three mollusc shells (Conomurex fasciatus, Ostrea edulis, Anomalocardia flexuosa). We employ an automated LIBS setup to map the Mg/Ca composition of whole shell sections with over 2,000 data points per hour. By assessing the spatial variability of Mg/Ca intensity ratios this method has the potential to mitigate distorted results while increasing the resolution of derived palaeoenvironmental information

Archaeological bone lipids as palaeodietary markers

Rationale Stable isotope analysis of archaeological and fossil bone samples can provide important insights into past environments, ecologies and diets. Previous studies have focused on stable carbon and nitrogen isotopes in bone collagen, or carbon isotopes in bone mineral (bioapatite). Carbon isotope analysis of lipids from archaeological bone has received much less attention, partly due to the lack of suitable methodologies allowing sufficient recovery of compounds for structural and isotopic characterisation. Here we show that lipids can be easily and reliably recovered from archaeological bone using a modified protocol, and that these provide complementary dietary information to other bone components. Methods Human and animal bones were obtained from a variety of archaeological contexts. Lipids were sequentially extracted using solvent extraction (dichloromethane/methanol), followed by acidified methanol extraction (methanol/H2SO4). The lipids were then analysed by gas chromatography/mass spectrometry (GC/MS) and gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). Results Appreciable amounts of endogenous lipid were recovered from archaeological bone. Importantly, a comparison between compound-specific and bulk collagen isotopic data shows that archaeological bone lipids reflect dietary input and can be used to distinguish between marine and terrestrial consumers, as well as between C3 and C4 plant consumers. Furthermore, the presence of essential fatty acids directly incorporated from diet to bone may provide additional palaeodietary information. Conclusions Our findings suggest that archaeological bone lipids are a hitherto untapped resource of dietary information that offer additional insights to those gained from other isotopic analyses of bone

Stable isotope evidence for dietary diversification in the pre-Columbian Amazon

Unidad de excelencia María de Maeztu CEX2019-000940-MArchaeological research is radically transforming the view that the Amazon basin and surrounding areas witnessed limited societal development before European contact. Nevertheless, uncertainty remains on the nature of the subsistence systems and the role that aquatic resources, terrestrial mammalian game, and plants had in supporting population growth, geographic dispersal, cultural adaptations and political complexity during the later stages of the pre-Columbian era. This is exacerbated by the general paucity of archaeological human remains enabling individual dietary reconstructions. Here we use stable carbon and nitrogen isotope analysis of bone collagen to reconstruct the diets of human individuals from São Luís Island (Brazilian Amazon coast) dated between ca. 1800 and 1000 cal BP and associated with distinct ceramic traditions. We expanded our analysis to include previously published data from Maracá and Marajó Island, in the eastern Amazon. Quantitative estimates of the caloric contributions from food groups and their relative nutrients using a Bayesian Mixing Model revealed distinct subsistence strategies, consisting predominantly of plants and terrestrial mammals and variably complemented with aquatic resources. This study offers novel quantitative information on the extent distinct food categories of polyculture agroforestry systems fulfilled the caloric and protein requirements of Late Holocene pre-Columbian populations in the Amazon basin

The loess deposits of Buca Dei Corvi section (Central Italy): Revisited

Loess deposits have been described in the past for the upper section of Buca Dei Corvi succession (Central Italy). In this paper the depositswere re-analysed to clarify the depositional environment and to attempt a paleoclimate reconstruction. Two radiocarbon dates on pedogenic carbonate constrain the ages to the Late Glacial, and are consistent with previous OSL dating of the top of the succession. The non-marine mollusc assemblage shows typical character of cold and dry climatic conditions, testified by strong oligotypical composition. Mineralogy and geochemistry of the sediments indicate the abundant presence of exotic quartz mineral which can be explained only by wind transport. Probably,wind transportwas also responsible of deposition of carbonatewhich then dissolved and re-precipitated producing pedogenic concretions. Stable isotopes (13C/12C and 18O/16O ratios) of the concretions are consistent with a climate drier than present conditions, with an environment characterized by sparse vegetation

New criteria for the molecular identification of cereal grains associated with archaeological artefacts.

The domestication and transmission of cereals is one of the most fundamental components of early farming, but direct evidence of their use in early culinary practices and economies has remained frustratingly elusive. Using analysis of a well-preserved Early Bronze Age wooden container from Switzerland, we propose novel criteria for the identification of cereal residues. Using gas chromatography mass spectrometry (GC-MS), we identified compounds typically associated with plant products, including a series of phenolic lipids (alkylresorcinols) found only at appreciable concentration in wheat and rye bran. The value of these lipids as cereal grain biomarkers were independently corroborated by the presence of macrobotanical remains embedded in the deposit, and wheat and rye endosperm peptides extracted from residue. These findings demonstrate the utility of a lipid-based biomarker for wheat and rye bran and offer a methodological template for future investigations of wider range of archaeological contexts. Alkylresorcinols provide a new tool for residue analysis which can help explore the spread and exploitation of cereal grains, a fundamental component of the advent and spread of farming

Shell sclerochronology and stable isotopes of the bivalve Anomalocardia flexuosa (Linnaeus, 1767) from southern Brazil: : implications for environmental and archaeological studies

We conduct the first stable isotopic and sclerochronological calibration of the bivalve Anomalocardia flexuosa (Linnaeus, 1767) in relation to environmental variables in a subtropical coastal area of southern Brazil. We investigate incremental shell growth patterns and δ18O and δ13C values of modern specimens collected alive from the Laguna Lagoonal System (LLS). As shells of Anomalocardia flexuosa are also the main components of pre-Columbian archaeological shell mounds and middens distributed along the Brazilian coastline, late Holocene archaeological specimens from a local shell mound (Cabeçuda) were selected to compare their stable carbon and oxygen isotopes with those of modern specimens. Shell growth increments, δ18O and δ13C values respond to a complex of environmental conditions, involving, for example, the effects of temperature and salinity. The isotopic information extracted from archaeological specimens from Cabeçuda shell midden in the LLS indirectly indicates that environmental conditions during the late Holocene were different from present day. In particular, intra-shell δ18O and δ13C values of archaeological shells reveal a stronger marine influence at 3 ka cal BP, which is in contrast to the seasonal freshwater/seawater balance that currently prevails at the LLS

Middle Holocene plant cultivation on the Atlantic Forest coast of Brazil?

This work provides robust oral pathology and stable isotope evidence on Bayesian mixing model for an unexpectedly high consumption of carbohydrates by a Middle Holocene coastal population of the Atlantic Forest of South America, an area traditionally viewed as peripheral to early centres of food production on the continent. A diversified economy with substantial consumption of plant resources was in place at the shellmound (or sambaqui) of Morro do Ouro, in Babitonga Bay, and supported a dense population at ca 4500 cal BP. This dietary composition is unique when compared with that of other contemporary and later groups in the region, including peoples who used ceramics and domesticated crops. The results corroborate independent dietary evidence, such as stone tool artefacts for plant processing and plant microremains in dental calculus of the same individuals, and suggest plant cultivation possibly took place in this region at the same time as the development of early agriculture in Amazonia and the La Plata Basin. Our study situates the Atlantic Forest coast of Brazil on the map of early plant management in the Neotropics

Dating human occupation and adaptation in the southern European last glacial refuge : The chronostratigraphy of Grotta del Romito (Italy)

Grotta del Romito has been the subject of numerous archaeological, chronological and palaeoenvironmental investigations for more than a decade. During the Upper Palaeolithic period the site contains evidence of human occupation through the Gravettian and Epigravettian periods, multiple human burials, changes in the pattern of human occupation, and faunal, isotopic and sedimentological evidence for local environmental change. In spite of this rich record, the chronological control is insufficient to resolve shifts in subsistence and mobility patterns at sufficiently high resolution to match the abrupt climate fluctuations at this time. To resolve this we present new radiocarbon and tephrostratigraphic dates in combination with existing radiocarbon dates, and develop a Bayesian age model framework for the site. This improved chronology reveals that local environmental conditions reflect abrupt and long-term changes in climate, and that these also directly influence changing patterns of human occupation of the site. In particular, we show that the environmental record for the site, based on small mammal habitat preferences, is chronologically in phase with the main changes in climate and environment seen in key regional archives from Italy and Greenland. We also calculate the timing of the transitions between different cultural phases and their spans. We also show that the intensification in occupation of the site is chronologically coincident with a rapid rise in Mesic Woody taxa seen in key regional pollen records and is associated with the Late Epigravettian occupation of the site. This change in the record of Grotta del Romito is also closely associated stratigraphically with a new tephra (the ROM-D30 tephra), which may act as a critical marker in environmental records of the region