Avaliação de desempenho : ferramenta para crescimento profissional

Orientador : Lis Andrea Pereira Soboll.Artigo (especialização) - Universidade Federal do Paraná, Setor de Ciências Sociais Aplicadas. Curso de Especialização MBA em Gestão de Talentos e Comportamento HumanoInclui referênciasResumo : O presente trabalho apresenta um estudo bibliográfico, apresentando as diversas formas de realizar avaliação de desempenho, seus modelos e métodos; e tem por objetivo verificar como a avaliação de desempenho se dá dentro das organizações, já que se supõe que uma avaliação de desempenho bem aplicada pode se tornar um instrumento para o crescimento profissional do empregado, bem como ser uma estratégia para o alcance dos objetivos organizacionais globais. Para tanto, foi realizado uma vasta pesquisa bibliográfica, explorando os diversos conceitos sobre o tema. Após o embasamento teórico, partiu-se para a verificação de como uma empresa do setor financeiro aplica determinado modelo de avaliação de desempenho e em seguida o trabalho aponta uma série de discussões sobre o modelo escolhido pela organização em estudo, apresentando seus pontos positivos e negativos. Por fim, conclui-se sobre o tema Avaliação de Desempenho, demonstrando a importância sobre a aplicação de conceitos e modelos para as organizações

Lysophosphatidylcholine: A Novel Modulator of Trypanosoma cruzi Transmission

Lysophosphatidylcholine is a bioactive lipid that regulates a large number of cellular processes and is especially present during the deposition and infiltration of inflammatory cells and deposition of atheromatous plaque. Such molecule is also present in saliva and feces of the hematophagous organism Rhodnius prolixus, a triatominae bug vector of Chagas disease. We have recently demonstrated that LPC is a modulator of Trypanosoma cruzi transmission. It acts as a powerful chemoattractant for inflammatory cells at the site of the insect bite, which will provide a concentrated population of cells available for parasite infection. Also, LPC increases macrophage intracellular calcium concentrations that ultimately enhance parasite invasion. Finally, LPC inhibits NO production by macrophages stimulated by live T. cruzi, and thus interferes with the immune system of the vertebrate host. In the present paper, we discuss the main signaling mechanisms that are likely used by such molecule and their eventual use as targets to block parasite transmission and the pathogenesis of Chagas disease

De Novo Assembly And Transcriptome Analysis Of The Rubber Tree (hevea Brasiliensis) And Snp Markers Development For Rubber Biosynthesis Pathways.

Hevea brasiliensis (Willd. Ex Adr. Juss.) Muell.-Arg. is the primary source of natural rubber that is native to the Amazon rainforest. The singular properties of natural rubber make it superior to and competitive with synthetic rubber for use in several applications. Here, we performed RNA sequencing (RNA-seq) of H. brasiliensis bark on the Illumina GAIIx platform, which generated 179,326,804 raw reads on the Illumina GAIIx platform. A total of 50,384 contigs that were over 400 bp in size were obtained and subjected to further analyses. A similarity search against the non-redundant (nr) protein database returned 32,018 (63%) positive BLASTx hits. The transcriptome analysis was annotated using the clusters of orthologous groups (COG), gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Pfam databases. A search for putative molecular marker was performed to identify simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). In total, 17,927 SSRs and 404,114 SNPs were detected. Finally, we selected sequences that were identified as belonging to the mevalonate (MVA) and 2-C-methyl-D-erythritol 4-phosphate (MEP) pathways, which are involved in rubber biosynthesis, to validate the SNP markers. A total of 78 SNPs were validated in 36 genotypes of H. brasiliensis. This new dataset represents a powerful information source for rubber tree bark genes and will be an important tool for the development of microsatellites and SNP markers for use in future genetic analyses such as genetic linkage mapping, quantitative trait loci identification, investigations of linkage disequilibrium and marker-assisted selection.9e10266

Protocolo de assistência do serviço de condicionamento físico para indivíduos com doenças neuromusculares e musculoesqueléticas

Physical fitness supervised by Physical Education professionals inserted in a rehabilitation program for patients with neuromuscular and musculoskeletal disorders aims to increase muscle strength, improve motor coordination, dynamic balance, aerobic capacity, functional capacity and health-related physical fitness. This is an important intervention to prevent and mitigate the synergy between loss of physical function and exacerbation of various comorbidities that compromise functional independence, modify risk factors, increase quality of life and longevity with reduced mortality. The aim of this paper is to present the assistance protocol of the Physical Conditioning Service of the Institute of Physical Medicine and Rehabilitation of the Clinical Hospital of the University of São Paulo Medical School (IMREA-HCFMUSP), designed for the intervention of physical exercises, respecting the profile and physical limitations of these patients, as well as observing their clinical markers.O condicionamento físico supervisionado por profissionais de Educação Física inserido em um programa de reabilitação para pacientes com distúrbios neuromusculares e musculoesqueléticos visa o aumento da força muscular, melhora da coordenação motora, equilíbrio dinâmico, capacidade aeróbia, capacidade funcional e da aptidão física voltada à saúde. Trata-se de importante intervenção para prevenir e atenuar a sinergia entre a perda de função física e a exacerbação de várias comorbidades que comprometem a independência funcional, modificar fatores de risco, aumentar a qualidade de vida e longevidade com redução da mortalidade. O objetivo desse artigo é apresentar o protocolo de assistência do Serviço de Condicionamento Físico do Instituto de Medicina Física e Reabilitação do Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (IMREA-HCFMUSP), elaborado para que a intervenção de exercícios físicos seja adequada, respeitando o perfil e limitações físicas desses pacientes, bem como na observação de seus marcadores clínicos

REMOÇÃO DE MICROCYSTIS AERUGINOSA E MICROCISTINA EM ÁGUA EUTROFIZADA ATRAVÉS DO PROCESSO COMBINADO DE COAGULAÇÃO/FLOCULAÇÃO SEGUIDO DE NANOFILTRAÇÃO

O processo de tratamento convencional é capaz de remover as células de cianobactérias, mas são pouco eficientes na remoção das cianotoxinas, necessitando de técnicas complementares para a remoção dessa fração dissolvida. Nesse contexto, a nanofiltração apresenta-se como tecnologia eficaz na remoção de cianotoxinas. Assim, a associação dos processos de coagulação/floculação/sedimentação (C/F/S) e nanofiltração (NF), como uma sequência de tratamento para águas oriundas de ambientes eutrofizados, torna-se relevante e, desta forma, foi adotada como objeto do presente estudo. Para os testes, foi preparada uma "água sintética", utilizando água deionizada e posterior contaminação com células de Microcystisaeruginosa, para obter concentração da ordem de 106 – 107céls/mL. A metodologia adotada neste trabalho foi realizada em duas etapas: 1) processo C/F/S usando os Tanfloc SG, SL e SS como coagulantes naturais para determinação da concentração ótima do coagulante 2) processo de NF utilizando as membranas NF-90 e NF-270, com características ligeiramente distintas, na pressão de 5 bar. O desempenho do tratamento como um todo C/F/S+NF, avaliado a partir dos parâmetros físico-químicos (turbidez, cor e pH) e microbiológicos (contagem de células de cianobactéria e concentração de microcistina-LR).AbstractThe conventional treatment process is capable of removing cyanobacteria cells but are inefficient in removing cyanotoxins, requiring additional techniques for removing dissolved fraction of that. In this context, nanofiltration, is presented as a technology effective in removing cyanotoxins and, for this reason, the combination of the processes of coagulation / flocculation / sedimentation (C / F / S) and nanofiltration (NF), as a result of treatment for waters originating from eutrophic environments, it becomes wide relevance, having been adopted as the object of this study. For the tests was prepared a "synthetic water", using deionized water and subsequent contamination with cells from Microcystisaeruginosa, to obtain concentration in the range 106-107 cells / mL. The methodology adopted in this study was performed in two steps: 1) Case C / F / S using Tanfloc SG, SL and SS as natural coagulants for determining the optimum concentration of coagulant 2) process using NF membranes NF-90 and NF -270 with slightly different characteristics, at a pressure of 5 bar. The performance of the treatment as a whole C / F / S + NF was evaluated from the physico-chemical parameters (turbidity, color and pH) and microbial (cyanobacterial cell counts and concentration of microcystin-LR)

Risk factors associated with death in Brazilian children with severe dengue: a case-control study

Objective: The purpose of this case-control study was to evaluate risk factors associated with death in children with severe dengue. Methods: The clinical condition of hospitalized patients with severe dengue who died (cases, n = 18) was compared with that of hospitalized patients with severe dengue who survived (controls, n = 77). The inclusion criteria for this study were age under 13 years; hospital admission in São Luis, northeastern Brazil; and laboratory-confirmed diagnosis of dengue. Results: Severe bleeding (hemoptysis), a defining criterion for dengue severity, was the factor most strongly associated with death in our study. We also found that epistaxis and persistent vomiting, both included as warning signs in the World Health Organization (WHO) classification of dengue, were strongly associated with death. No significant association was observed between any of the laboratory findings and death. Conclusions: The finding that epistaxis and persistent vomiting were also associated with death in children with severe dengue was unexpected and deserves to be explored in future studies. Because intensive care units are often limited in resource-poor settings, any information that can help to distinguish patients with severe dengue with a higher risk to progress to death may be crucial

Growth of Fungal Cells and the Production of Mycotoxins

Some filamentous fungi are able to grow in food and produce toxic metabolites. It occurs mainly in grains, cereals, oilseeds and some by-products. The growth of fungi in a particular food is governed largely by a series of physical and chemical parameters. The production of toxic metabolites is not confined to a single group of molds irrespective of whether they are grouped according to structure, ecology, or phylogenetic relationships. Mycotoxins can be carcinogenic and cause several harmful effects to both human and animal organisms, in addition to generating large economic losses. The major mycotoxins found in food are the aflatoxins, fumonisins, ochratoxins, patulin, zearalenone, and trichothecenes, generally stable at high temperatures and long storage periods. Considering the difficult prevention and control, international organizations for food safety establish safe levels of these toxins in food destined for both human and animal consumption. Good agricultural practices and control of temperature and moisture during storage are factors which contribute significantly to inhibit the production of mycotoxins. The use of some fungistatic products, such as essential oils and antioxidants, as well as physical, mechanical, chemical, or thermal processing, represents important methods to have the concentration of mycotoxins reduced in food