A proposito di nome, contenuti, programmi e prospettive per la nostra area culturale

[ES] Sin resumenCardone, V. (2005). A proposito di nome, contenuti, programmi e prospettive per la nostra area culturale. EGA. Revista de Expresión Gráfica Arquitectónica. (10):48-57. https://doi.org/10.4995/ega.2005.10329SWORD48571

EGA's succes amongst Italian academics

[EN] Since a long time I have been convinced that EGA is the best architectural representation magazine – or maybe of all graphic representation ones – and it’s not a mistery. It is obvious that not everyone will share this oppinion and someone could get angry when I say it, but it is meditated oppinion, supported by irrefutable data, released from my personal relationship with the magazine and the colleagues who participate in it.[IT] Da molti anni sono convinto che EGA sia la migliore rivista di rappresentazione dell’architettura – se non addirittura di tutte quelle che si interessano di rappresentazione grafica – e non ne faccio mistero. È ovvio che non tutti condividono e qualcuno si arrabbia quando lo affermo, ma si tratta di opinione meditata, suffragata da dati inoppugnabili, svincolati dal mio personale rapporto con la rivista e i colleghi che la curano.Cardone, V. (2018). Il successo di EGA presso gli studiosi italiani. EGA. Revista de Expresión Gráfica Arquitectónica. 23(34):206-211. doi:10.4995/ega.2018.10852SWORD2062112334AMORUSO, G., 2018. El espacio ilusorio barroco en las perspectivas arquitectónicas de Girolamo Curti y Angelo Michele Colonna en el Palacio de la Ciudad de Bolonia, EGA Expresión Gráfica Arquitectónica, 33, pp. 78-89. https://doi.org/10.4995/ega.2018.10389Barba, S., Messina, B. (in charge of), 2005. Il disegno dei viaggiatori. Salerno: CUES.Bartolomei, C., Ippolito, A., 2015. Faros italianos entre geometría y simbolismo, EGA Expresión Gráfica Arquitectónica, 25, pp. 192-199. https://doi.org/10.4995/ega.2015.3679Borja Molero, A., Barba, S., Álvaro Tordesillas, A., 2016., Documentación del patrimonio cultural. Método basado en la fusión de técnicas fotogramétricas y de escaneado óptico de triangulación, EGA Expresión Gráfica Arquitectónica, 28, pp. 236-245. https://doi.org/10.4995/ega.2016.6308Cabezos Bernal, P., Rossi, A., 2017. Técnicas de musealización virtual. Los capiteles del Monasterio de San Cugat, EGA Expresión Gráfica Arquitectónica, 29, pp. 48-57. https://doi.org/10.4995/ega.2017.7340Calisi, D., Cianci, M.G., 2018. De lo virtual a lo real. Un modelo de madera para la reconstrucción filológica del barrio Alessandrino en la zona arqueológica central de Roma, EGA Expresión Gráfica Arquitectónica, 33, pp. 90-102. https://doi.org/10.4995/ega.2018.8924Capone, M., Nigro, E., 2017. Desde la geometría hasta la representación generativa. La búsqueda de una solución optimizada en el proyecto del Club Táchira (Caracas, 1955), E GA Expresión Gráfica Arquitectónica, 31, pp. 172-183.Cardone, V., 2002. Pedro Luis Escrivá: forma e funzione nel disegno delle fortificazioni, EGA Expresión Gráfica Arquitectónica, 7, pp. 36-47.Cardone, V., 2003. Pedro Luis Escrivá, ingegnere militare del Regno di Napoli. Salerno: CUES.Cardone, V., 2005. A proposito di nome, contenuti, programmi e prospettive per la nostra area culturale, EGA Expresión Gráfica Arquitectónica, 10, pp. 48-57. https://doi.org/10.4995/ega.2005.10329Cardone, V., 2017. Editoriale, diségno, n.1, luglio-dicembre 2017, pp. 5-8.Cipriani, L., Fantini, F., Bertacchi, S., 2015. El color en las piedras y en los mosaicos de Rávena: nuevas imágenes de los monumentos antiguos a través de la fotogrametría no convencional de última generación, EGA Expresión Gráfica Arquitectónica, 26, pp. 190-201. https://doi.org/10.4995/ega.2015.4052De Sanctis, A., Fortunato, G., Zappani, A.A., 2017. Nuevos levantamientos y documentos de archivo para el conocimiento de los bienes arquitectónicos: la construcción en el siglo xvii de un nuevo vestíbulo en el Convento- Santuario de San Francisco de Paula (Paula- Italia), EGA Expresión Gráfica Arquitectónica, 30, pp. 118-129. https://doi.org/10.4995/ega.2017.7835Fantini, F., 2012. Modelos con nivel de detalle variable realizados mediante un levantamiento digital aplicados a la arqueología, EGA Expresión Gráfica Arquitectónica, 19, pp. 306-317. https://doi.org/10.4995/ega.2012.1383Gombrich, E.H., 1993. Historia del arte y psicología en Viena, hace cincuenta años, EGA Expresión Gráfica Arquitectónica, 1, pp. 38-41.Leserri, M., Rossi, G., 2013. Arquitecturas de piedra seca, un levantamiento problemático, EGA Expresión Gráfica Arquitectónica, 22, pp. 184-195. https://doi.org/10.4995/ega.2013.1532Maestri, D., 1993. Axonometría, dibujo y arquitectura, EGA Expresión Gráfica Arquitectónica, 1, pp. 99-108.Rossi, A., Palmero, L., DeGregorio, S., 2018. El análisis de la forma en el diseño arquitectónico: desde el proyecto a la ejecución, EGA Expresión Gráfica Arquitectónica, 32, pp. 186-197. https://doi.org/10.4995/ega.2018.894

Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

<p>Abstract</p> <p>Objective</p> <p>Cholesterol changes have been described in prion-cell models and in experimental rodent scrapie; yet, the pattern of this association is still controversial.</p> <p>Methods</p> <p>To shed light on the matter, we analysed and compared cholesterol variations in ScN2a cells and in brains of Scrapie-infected C57Bl/6 mice, using two different methods: a fluorimetric-enzymatic cholesterol assay, and high performance liquid chromatography-mass spectroscopy (HPLC-MS).</p> <p>Results</p> <p>Compared to uninfected controls, similar cholesterol metabolism anomalies were observed in infected cells and brains by both methods; however, only HPLC-MS revealed statistically significant cholesterol variations, particularly in the cholesteryl esters (CE) fraction. HPLC-MS analyses also revealed different fatty acid composition of the CE fraction in cells and brains. In N2a cells, their profile reflected that of serum, while in normal brains cholesteryl-linoleate only was found at detectable levels. Following prion infection, most CE species were increased in the CE pool of ScN2a cells, whereas a conspicuous amount of cholesteryl-arachidonate only was found to contribute to the cerebral increase of CE. Of interest, oral pravastatin administration to Scrapie-infected mice, was associated with a significant reduction of cerebral free cholesterol (FC) along with a concomitant further increase of the CE pool, which included increased amounts of both cholesteryl-linoleate and cholesteryl-arachidonate.</p> <p>Conclusion</p> <p>Although mechanistic studies are needed to establish the pathophysiological relevance of changes in cerebral CE concentrations, to the best of our knowledge this is the first report to provide evidence of increased cholesterol esterification in brains of prion-infected mice, untreated and treated with pravastatin.</p

Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish

BACKGROUND: Scrapie and bovine spongiform encephalopathy (BSE) belongs to the group of animal transmissible spongiform encephalopathy (TSE). BSE epidemic in the UK and elsewhere in Europe has been linked to the use of bovine meat and bone meals (MBM) in the feeding of cattle. There is concern that pigs, poultry and fish bred for human consumption and fed with infected MBM would eventually develop BSE or carry residual infectivity without disease. Although there has been no evidence of infection in these species, experimental data on the susceptibility to the BSE agent of farm animals other than sheep and cow are limited only to pigs and domestic chicken. In the framework of a EU-granted project we have challenged two species of fish largely used in human food consumption, rainbow trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus), with a mouse-adapted TSE strain (scrapie 139A), to assess the risk related to oral consumption of TSE contaminated food. In trout, we also checked the "in vitro" ability of the pathological isoform of the mouse prion protein (PrP(Sc)) to cross the intestinal epithelium when added to the mucosal side of everted intestine. RESULTS: Fish challenged with a large amount of scrapie mouse brain homogenate by either oral or parenteral routes, showed the ability to clear the majority of infectivity load. None of the fish tissues taken at different time points after oral or parenteral inoculation was able to provoke scrapie disease after intracerebral inoculation in recipient mice. However, a few recipient mice were positive for PrP(Sc )and spongiform lesions in the brain. We also showed a specific binding of PrP(Sc )to the mucosal side of fish intestine in the absence of an active uptake of the prion protein through the intestinal wall. CONCLUSION: These results indicate that scrapie 139A, and possibly BSE, is quickly removed from fish tissues despite evidence of a prion like protein in fish and of a specific binding of PrP(Sc )to the mucosal side of fish intestine

Cetuximab continuation after first progression in metastatic colorectal cancer (CAPRI-GOIM): A randomized phase II trial of FOLFOX plus cetuximab versus FOLFOX

Background: Cetuximab plus chemotherapy is a first-line treatment option in metastatic KRAS and NRAS wild-type colorectal cancer (CRC) patients. No data are currently available on continuing anti-epidermal growth factor receptor (EGFR) therapy beyond progression. Patients and methods: We did this open-label, 1:1 randomized phase II trial at 25 hospitals in Italy to evaluate the efficacy of cetuximab plus 5-fluorouracil, folinic acid and oxaliplatin (FOLFOX) as second-line treatment of KRAS exon 2 wild-type metastatic CRC patients treated in first line with 5-fluorouracil, folinic acid and irinotecan (FOLFIRI) plus cetuximab. Patients received FOLFOX plus cetuximab (arm A) or FOLFOX (arm B). Primary end point was progressionfree survival (PFS). Tumour tissues were assessed by next-generation sequencing (NGS). This report is the final analysis. Results: Between 1 February 2010 and 28 September 2014, 153 patients were randomized (74 in arm A and 79 in arm B). Median PFS was 6.4 [95% confidence interval (CI) 4.7-8.0] versus 4.5 months (95% CI 3.3-5.7); [hazard ratio (HR), 0.81; 95% CI 0.58-1.12; P = 0.19], respectively. NGS was performed in 117/153 (76.5%) cases; 66/117 patients (34 in arm A and 32 in arm B) had KRAS, NRAS, BRAF and PIK3CA wild-type tumours. For these patients, PFS was longer in the FOLFOX plus cetuximab arm [median 6.9 (95% CI 5.5-8.2) versus 5.3 months (95% CI 3.7-6.9); HR, 0.56 (95% CI 0.33-0.94); P = 0.025]. There was a trend in better overall survival: median 23.7 [(95% CI 19.4-28.0) versus 19.8 months (95% CI 14.9-24.7); HR, 0.57 (95% CI 0.32-1.02); P = 0.056]. Conclusions: Continuing cetuximab treatment in combination with chemotherapy is of potential therapeutic efficacy in molecularly selected patients and should be validated in randomized phase III trials

Association of kidney disease measures with risk of renal function worsening in patients with type 1 diabetes

Background: Albuminuria has been classically considered a marker of kidney damage progression in diabetic patients and it is routinely assessed to monitor kidney function. However, the role of a mild GFR reduction on the development of stage 653 CKD has been less explored in type 1 diabetes mellitus (T1DM) patients. Aim of the present study was to evaluate the prognostic role of kidney disease measures, namely albuminuria and reduced GFR, on the development of stage 653 CKD in a large cohort of patients affected by T1DM. Methods: A total of 4284 patients affected by T1DM followed-up at 76 diabetes centers participating to the Italian Association of Clinical Diabetologists (Associazione Medici Diabetologi, AMD) initiative constitutes the study population. Urinary albumin excretion (ACR) and estimated GFR (eGFR) were retrieved and analyzed. The incidence of stage 653 CKD (eGFR &lt; 60 mL/min/1.73 m2) or eGFR reduction &gt; 30% from baseline was evaluated. Results: The mean estimated GFR was 98 \ub1 17 mL/min/1.73m2 and the proportion of patients with albuminuria was 15.3% (n = 654) at baseline. About 8% (n = 337) of patients developed one of the two renal endpoints during the 4-year follow-up period. Age, albuminuria (micro or macro) and baseline eGFR &lt; 90 ml/min/m2 were independent risk factors for stage 653 CKD and renal function worsening. When compared to patients with eGFR &gt; 90 ml/min/1.73m2 and normoalbuminuria, those with albuminuria at baseline had a 1.69 greater risk of reaching stage 3 CKD, while patients with mild eGFR reduction (i.e. eGFR between 90 and 60 mL/min/1.73 m2) show a 3.81 greater risk that rose to 8.24 for those patients with albuminuria and mild eGFR reduction at baseline. Conclusions: Albuminuria and eGFR reduction represent independent risk factors for incident stage 653 CKD in T1DM patients. The simultaneous occurrence of reduced eGFR and albuminuria have a synergistic effect on renal function worsening