Determination of masses of the central black holes in NGC524 and NGC2549 using Laser Guide Star Adaptive Optics

[abridged] We present observations of NGC524 and NGC2549 with LGS AO obtained at GEMINI North telescope using the NIFS IFU in the K band. The purpose of these observations, together with previously obtained observations with the SAURON IFU, is to determine the masses (Mbh) of the supermassive black holes (SMBH). The targeted galaxies were chosen to have central light profiles showing a core (NGC524) and a cusp (NGC2549), to probe the feasibility of using the galaxy centre as the NGS required for LGS AO. We employ an innovative `open loop' technique. The data have spatial resolution of 0.23" and 0.17" FWHM, showing that high quality LGS AO observations of these objects are possible. We construct axisymmetric three-integral dynamical models which are constrained with both the NIFS and SAURON data. The best fitting models yield Mbh=(8.3 +2.7 -1.3) x 10^8 Msun for NGC524 and Mbh=(1.4 +0.2 -1.3) x 10^7 Msun for NGC2549 (all errors are at the 3 sigma CL). We demonstrate that the wide-field SAURON data play a crucial role in the M/L determination increasing the accuracy of M/L by a factor of at least 5, and constraining the upper limits on Mbh. The NIFS data are crucial in constraining the lower limits of Mbh and in combination with the large scale data reducing the uncertainty by a factor of 2 or more. We find that the orbital structure of NGC524 shows significant tangential anisotropy, while at larger radii both galaxies are consistent with having almost perfectly oblate velocity ellipsoids. Tangential anisotropy in NGC524 coincides with the size of SMBH sphere of influence and the core region in the light profile. We test the accuracy to which Mbh can be measured using seeings obtained from typical LGS AO observations, and conclude that for a typical conditions and Mbh the expected uncertainty is of the order of 50%.Comment: 19 pages, 14 figure

Functional rescue of dystrophin deficiency in mice caused by frameshift mutations using Campylobacter jejuni Cas9

Duchenne muscular dystrophy (DMD) is a fatal, X-linked muscle wasting disease caused by mutations in the DMD gene. In 51% of DMD cases, a reading frame is disrupted because of deletion of several exons. Here, we show that CjCas9 derived from Campylobacter jejuni can be used as a gene editing tool to correct an out-of-frame Dmd exon in Dmd knockout mice. Herein, we used Cas9 derived from S. pyogenes to generate Dmd knockout (KO) mice with a frameshift mutation in Dmd gene. Then, we expressed CjCas9, its single-guide RNA, and the eGFP gene in the tibialis anterior muscle of the Dmd KO mice using an all-in-one adeno-associated virus (AAV) vector. CjCas9 cleaved the target site in the Dmd gene efficiently in vivo and induced small insertions or deletions at the target site. This treatment resulted in conversion of the disrupted Dmd reading frame from out-of-frame to in-frame, leading to the expression of dystrophin in the sarcolemma. Importantly, muscle strength was enhanced in the CjCas9-treated muscles, without off-target mutations, indicating high efficiency and specificity of CjCas9. This work suggests that in vivo DMD frame correction, mediated by CjCas9 has great potential for the treatment of DMD and other neuromuscular diseases

Galaxy Masses

Galaxy masses play a fundamental role in our understanding of structure formation models. This review addresses the variety and reliability of mass estimators that pertain to stars, gas, and dark matter. The different sections on masses from stellar populations, dynamical masses of gas-rich and gas-poor galaxies, with some attention paid to our Milky Way, and masses from weak and strong lensing methods, all provide review material on galaxy masses in a self-consistent manner.Comment: 145 pages, 28 figures, to appear in Reviews of Modern Physics. Figure 22 is missing here, and Figs. 15, 26-28 are at low resolution. This version has a slightly different title and some typos fixed in Chapter 5. For the full review with figures, please consult: http://www.astro.queensu.ca/~courteau/GalaxyMasses_28apr2014.pd

A SAURON look at galaxy bulges

Kinematic and population studies show that bulges are generally rotationally flattened systems similar to low-luminosity ellipticals. However, observations with state-of-the-art integral field spectrographs, such as SAURON, indicate that the situation is much more complex, and allow us to investigate phenomena such as triaxiality, kinematic decoupling and population substructure, and to study their connection to current formation and evolution scenarios for bulges of early-type galaxies. We present the examples of two S0 bulges from galaxies in our sample of nearby galaxies: one that shows all the properties expected from classical bulges (NGC5866), and another case that presents kinematic features appropriate for barred disk galaxies (NGC7332).Comment: 4 pages, 3 figures, accepted for publishing in AN (refereed conf. proc. of the Euro3D Science workshop, IoA Cambridge, May 2003

Correlations Between Central Massive Objects And Their Host Galaxies: From Bulgeless Spirals to Ellipticals

Recent observations by Ferrarese et al. (2006) and Wehner et al. (2006) reveal that a majority of galaxies contain a central massive object (CMO), either a supermassive black hole (SMBH) or a compact stellar nucleus, regardless of the galaxy mass or morphological type, and that there is a tight relation between the masses of CMOs and those of the host galaxies. Several recent studies show that feedback from black holes can successfully explain the \msigma correlation in massive elliptical galaxies that contain SMBHs. However, puzzles remain in spirals or dwarf spheroids that do not appear to have black holes but instead harbor a compact central stellar cluster. Here we use three-dimensional, smoothed particle hydrodynamics simulations of isolated galaxies to study the formation and evolution of CMOs in bulgeless disk galaxies, and simulations of merging galaxies to study the transition of the CMO--host mass relation from late-type bulgeless spirals to early-type ellipticals. Our results suggest that the observed correlations may be established primarily by the depletion of gas in the central region by accretion and star-formation, and may hold for all galaxy types. A systematic search for CMOs in the nuclei of bulgeless disk galaxies would offer a test of this conclusion. (Abridged)Comment: 11 pages, 8 figures, accepted to Ap

SAURON Observations of Disks in Spheroids

The panoramic integral-field spectrograph SAURON is currently being used to map the stellar kinematics, gaseous kinematics, and stellar populations of a large number of early-type galaxies and bulges. Here, we describe SAURON observations of cold stellar disks embedded in spheroids (NGC3384, NGC4459, NGC4526), we illustrate the kinematics and ionization state of large-scale gaseous disks (NGC4278, NGC7742), and we show preliminary comparisons of SAURON data with barred galaxy N-body simulations (NGC3623).Comment: 8 pages including 5 figures. To appear in Galaxies: The Third Dimension, eds. M. Rosado, L. Binnette, & L. Arias (ASP: San Francisco

SAURON: An Innovative Look at Early-Type Galaxies

A summary of the SAURON project and its current status is presented. SAURON is a panoramic integral-field spectrograph designed to study the stellar kinematics, gaseous kinematics, and stellar populations of spheroids. Here, the sample of galaxies and its properties are described. The instrument is detailed and its capabilities illustrated through observational examples. These includes results on the structure of central stellar disks, the kinematics and ionization state of gaseous disks, and the stellar populations of galaxies with decoupled cores.Comment: 10 pages, 6 figures. To appear in "The Dynamics, Structure & History of Galaxies", eds. G. S. Da Costa & E. M. Sadler (San Francisco: ASP). Version with full resolution images available at http://www.strw.leidenuniv.nl/~dynamics/Instruments/Sauron/pub_list.htm

Towards a new classification of early-type galaxies: an integral-field view

In this proceeding we make use of the two-dimensional stellar kinematics of a representative sample of E and S0 galaxies obtained with the SAURON integral-field spectrograph to reveal that early-type galaxies appear in two broad flavours, depending on whether they exhibit clear large-scale rotation or not. We measure the level of rotation via a new parameter LambdaR and use it as a basis for a new kinematic classification that separates early-type galaxies into slow and fast rotators. With the aid of broad-band imaging we will reinforce this finding by comparing our kinematic results to the photometric properties of these two classes.Comment: 4 pages, 2 figures, to appear in "Pathways Through an Eclectic Universe", J. H. Knappen, T. J. Mahoney, and A. Vazedekis (Eds.), ASP Conf. Ser., 200

A SAURON view of galaxies

We have measured the two-dimensional kinematics and line-strength distributions of 72 representative nearby early-type galaxies, out to approximately one effective radius, with our panoramic integral-field spectrograph SAURON. The resulting maps reveal a rich variety in kinematical structures and linestrength distributions, indicating that early-type galaxies are more complex systems than often assumed. We are building detailed dynamical models for these galaxies, to derive their intrinsic shape and dynamical structure, and to determine the mass of the supermassive central black hole. Here we focus on two examples, the compact elliptical M32 and the E3 galaxy NGC4365. These objects represent two extreme cases: M32 has very regular kinematics which can be represented accurately by an axisymmetric model in which all stars rotate around the short axis, while NGC4365 is a triaxial galaxy with a prominent kinematically decoupled core, with an inner core that rotates about an axis that is nearly perpendicular to the rotation axis of the main body of the galaxy. Our dynamical models for these objects demonstrate that two-dimensional observations are essential for deriving the intrinsic orbital structure and dark matter content of galaxies.Comment: 7 pages (3 figures, full resolution Fig. 1 available at http://www.strw.leidenuniv.nl/~verolme/M32.ps). Contributed talk to the Athens Workshop on Galaxies and Chaos, Theory and Observations; Proceedings to appear in "Galaxies and Chaos", eds. G. Contopoulos and N. Vogli