Establishing a Community of Practice for Doctoral Studies Amidst the COVID-19 Pandemic

In this discussion paper, we describe our experience completing the first year of the doctorate in nursing program at a large urban academic centre during the COVID-19 pandemic. We highlight the current nursing shortage and the importance of supporting all nursing students, including nurses in doctoral programs, towards successful graduation. We describe the development of a virtual community of practice incorporating five key strategies: Building community, fostering collaboration, strengthening connection, enhancing creativity, and promoting consistency. We believe that utilizing these strategies will contribute to our success and may be relevant to nursing leaders seeking to support the development of more doctorally prepared nurses. Participation in a community of practice early on in doctoral education will not only better prepare students for success in their program, but also continued success as they progress through their careers. It is important for students to not only make connections with peers in their area of academic study, but to also reach out to peers in other disciplines to improve both individual and interdisciplinary growth. Program administrators and educators can encourage the formation of community of practice among novice doctoral students. This encouragement can be achieved using a virtual platform, or in-person networking opportunities. Inviting incoming graduate students to connect with each other and with students from previous cohorts also fosters community of practice formation. Résumé Dans ce texte de discussion, nous décrivons notre expérience relative à la première année du doctorat en sciences infirmières dans un grand centre universitaire urbain pendant la pandémie de COVID-19. Nous soulignons la pénurie actuelle d’infirmières et l’importance de soutenir toutes les étudiantes en sciences infirmières, y compris les infirmières inscrites à un programme de doctorat, vers la réussite de leurs études. Nous décrivons le développement d’une communauté virtuelle de pratique intégrant cinq stratégies clés : créer une communauté, favoriser la collaboration, renforcer les liens, mettre en valeur la créativité et promouvoir la cohérence. Nous pensons que l’utilisation de ces stratégies contribuera à notre succès et pourrait être pertinente pour les infirmières chefs de file qui cherchent à soutenir le développement d’un plus grand nombre d’infirmières préparées au doctorat. La participation à une communauté de pratique dès le début de la formation doctorale permettra non seulement de mieux préparer les étudiantes à réussir dans leur programme, mais favorisera également leur poursuite d’une carrière fructueuse. Il est important pour les étudiantes d’établir des liens non seulement avec des pairs dans leur domaine d’études universitaires, mais qu’ils entrent également en contact avec des pairs d’autres disciplines pour améliorer à la fois leur croissance individuelle et interdisciplinaire. Les administratrices de programme et les enseignantes peuvent encourager la formation d’une communauté de pratique parmi les doctorantes novices. Cet encouragement peut être réalisé à l’aide d’une plateforme virtuelle ou d’occasions de réseautage en personne. Inviter les étudiantes diplômées à entrer en contact les unes avec les autres et avec les étudiantes des cohortes précédentes favorise également la formation d’une communauté de pratique

Phosphodiesterase 10A Upregulation Contributes to Pulmonary Vascular Remodeling

Phosphodiesterases (PDEs) modulate the cellular proliferation involved in the pathophysiology of pulmonary hypertension (PH) by hydrolyzing cAMP and cGMP. The present study was designed to determine whether any of the recently identified PDEs (PDE7-PDE11) contribute to progressive pulmonary vascular remodeling in PH. All in vitro experiments were performed with lung tissue or pulmonary arterial smooth muscle cells (PASMCs) obtained from control rats or monocrotaline (MCT)-induced pulmonary hypertensive (MCT-PH) rats, and we examined the effects of the PDE10 inhibitor papaverine (Pap) and specific small interfering RNA (siRNA). In addition, papaverine was administrated to MCT-induced PH rats from day 21 to day 35 by continuous intravenous infusion to examine the in vivo effects of PDE10A inhibition. We found that PDE10A was predominantly present in the lung vasculature, and the mRNA, protein, and activity levels of PDE10A were all significantly increased in MCT PASMCs compared with control PASMCs. Papaverine and PDE10A siRNA induced an accumulation of intracellular cAMP, activated cAMP response element binding protein and attenuated PASMC proliferation. Intravenous infusion of papaverine in MCT-PH rats resulted in a 40%–50% attenuation of the effects on pulmonary hypertensive hemodynamic parameters and pulmonary vascular remodeling. The present study is the first to demonstrate a central role of PDE10A in progressive pulmonary vascular remodeling, and the results suggest a novel therapeutic approach for the treatment of PH

Table 1: Cannabis Survey Results

Supplemental data for, 'Receptiveness to participating in cannabis research in pregnancy: a survey study at The Ottawa Hospital'

Current methods for the isolation of extracellular vesicles

Extracellular vesicles (EVs), including microvesicles and exosomes, are nano- to micron-sized vesicles, which may deliver bioactive cargos that include lipids, growth factors and their receptors, proteases, signaling molecules, as well as mRNA and non-coding RNA, released from the cell of origin, to target cells. EVs are released by all cell types and likely induced by mechanisms involved in oncogenic transformation, environmental stimulation, cellular activation, oxidative stress, or death. Ongoing studies investigate the molecular mechanisms and mediators of EVs-based intercellular communication at physiological and oncogenic conditions with the hope of using this information as a possible source for explaining physiological processes in addition to using them as therapeutic targets and disease biomarkers in a variety of diseases. A major limitation in this evolving discipline is the hardship and the lack of standardization for already challenging techniques to isolate EVs. Technical advances have been accomplished in the field of isolation with improving knowledge and emerging novel technologies, including ultracentrifugation, microfluidics, magnetic beads and filtration-based isolation methods. In this review, we will discuss the latest advances in methods of isolation methods and production of clinical grade EVs as well as their advantages and disadvantages, and the justification for their support and the challenges that they encounter

Adult height and the risk of cause-specific death and vascular morbidity in 1 million people: individual participant meta-analysis.

BACKGROUND: The extent to which adult height, a biomarker of the interplay of genetic endowment and early-life experiences, is related to risk of chronic diseases in adulthood is uncertain. METHODS: We calculated hazard ratios (HRs) for height, assessed in increments of 6.5 cm, using individual-participant data on 174374 deaths or major non-fatal vascular outcomes recorded among 1085949 people in 121 prospective studies. RESULTS: For people born between 1900 and 1960, mean adult height increased 0.5-1 cm with each successive decade of birth. After adjustment for age, sex, smoking and year of birth, HRs per 6.5 cm greater height were 0.97 (95% confidence interval: 0.96-0.99) for death from any cause, 0.94 (0.93-0.96) for death from vascular causes, 1.04 (1.03-1.06) for death from cancer and 0.92 (0.90-0.94) for death from other causes. Height was negatively associated with death from coronary disease, stroke subtypes, heart failure, stomach and oral cancers, chronic obstructive pulmonary disease, mental disorders, liver disease and external causes. In contrast, height was positively associated with death from ruptured aortic aneurysm, pulmonary embolism, melanoma and cancers of the pancreas, endocrine and nervous systems, ovary, breast, prostate, colorectum, blood and lung. HRs per 6.5 cm greater height ranged from 1.26 (1.12-1.42) for risk of melanoma death to 0.84 (0.80-0.89) for risk of death from chronic obstructive pulmonary disease. HRs were not appreciably altered after further adjustment for adiposity, blood pressure, lipids, inflammation biomarkers, diabetes mellitus, alcohol consumption or socio-economic indicators. CONCLUSION: Adult height has directionally opposing relationships with risk of death from several different major causes of chronic diseases