Radiological study of reconstruction of the anterior cruciate ligament by transtibial approach

Introduction: the rupture of the anterior cruciate ligament (ACL) is often due to sports activities and its reconstruction methods have undergone constant changes due to improvements in the surgical techniques. Objective: To evaluate the radiological placement of the tibial and femoral tunnels using the transtibial technique assisted by the femoral pre-drilling. Method: Radiological analysis (AP and lateral), at 4 weeks postoperatively in 98 patients, totaling 100 cases of ACL reconstruction. Three examiners evaluated the placement of the tibial and femoral tunnels. Methods for assessing the positioning of the tunnels were: Scanlan, Staubli and Rauschning and Bernard. Results: the a angle (AP) was 64.13 degrees (+/- 4.29 degrees) and the beta angle (lateral) was 57.28 degrees (+/- 4.41 degrees). the mean tibial positioning was 41.99% (+/- 5.14%). the ACL graft was inserted into the lateral condyle of the femur and the average percentage of radiographic positioning in the green quadrant was 62%, the yellow quadrant, 37% and 1% in the red quadrant. Conclusions: the transtibial technique for ACL reconstruction, assisted by the femoral pre-drilling provides the anatomical position of the graft in the majority of the cases, as radiological evidence.Hosp Coracao HCor, Inst Joelho, São Paulo, SP, BrazilInst Assistencia Med Servidor Publ Estadual IAMSP, São Paulo, SP, BrazilUniversidade Federal de São Paulo Unifesp, São Paulo, SP, BrazilUniversidade Federal de São Paulo Unifesp, São Paulo, SP, BrazilWeb of Scienc

Efeitos da Familiaridade da Palavra no Desenvolvimento de Ditado em Leitores Iniciantes

A nomeação de figuras é uma das habilidades componentes da rede de relações comportamentais que caracteriza os repertórios de leitura e escrita de acordo com o paradigma de equivalência de estímulos. Sendo assim, o processo de aprendizagem de leitura e escrita de nomes de objetos do cotidiano do estudante poderia ser mais eficiente do que a aquisição envolvendo palavras desconhecidas ou pseudopalavras. O presente estudo avaliou se a familiaridade da palavra afeta a emergência da escrita sob controle de ditado em um procedimento de ensino de seleção da palavra impressa a partir de palavra ditada. Dez alunos com idades entre sete e dez anos, ainda não alfabetizados, realizaram sessões de ensino informatizado e verificou-se que o número de sessões até o critério aumentou progressivamente de acordo com o tipo de palavras – familiares, não-familiares e pseudopalavras. Todos os estudantes aprenderam a ler as palavras com 100% de acertos; a escrita emergiu gradualmente, iniciando por composições aleatórias, passando por construções com omissões, inclusões ou trocas, até correção completa, para a maioria dos alunos. Os resultados confirmam que a familiaridade das palavras é uma variável relevante para a aprendizagem de leitura e estendem essa relação para a aprendizagem da escrita.Palavras-chave: ensino; leitura; escrita; equivalência de estímulos; familiaridade

OSTEOCHONDRAL AUTOLOGOUS TRANSPLANTATION FOR TREATING CHONDRAL LESIONS IN THE PATELLA

ABSTRACTObjective: The primary aim of this study was to assess the clinical and functional evolution of patients with total-thickness symptomatic cartilaginous injury of the patellar joint surface, treated by means of osteochondral autologous transplantation. Methods: This prospective study was conducted from June 2008 to March 2011 and involved 17 patients. The specific questionnaires of Lysholm, Kujala and Fulkerson were completed preoperatively and one year postoperatively in order to assess the affected knee, and SF-36 was used to assess these patients’ general quality of life. The nonparametric paired Wilcoxon test was used for statistical analysis on the pre and postoperative questionnaires. The data were analyzed using the SPSS for Windows software, version 16.0, and a significance level of 5% was used. Results: The Lysholm preoperative and postoperative average scores were 54.59 and 75.76 points (p < 0.05). The Fulkerson pre and postoperative average scores were 52.53 and 78.41 points (p < 0.05). Conclusions: We believe that autologous osteochondral transplantation is a good treatment method for total-thickness symptomatic chondral lesions of the joint surface of the patella

Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing

OBJECTIVES: The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures. METHODS: A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. RESULTS: Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group. CONCLUSION: Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Universidade Federal do Vale do Sao Francisco Colegiado de MedicinaUniversidade Federal de São Paulo (UNIFESP) Departamento de CirurgiaUniversidade Federal do Vale do Sao Francisco Colegiado de Medicina VeterinariaSociedade Brasileira de Traumatologia Esportiva e ArtroscopiaUNIFESP, Depto. de Cirurgia104698SciEL

Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing

OBJECTIVES: The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures. METHODS: A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. RESULTS: Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group. CONCLUSION: Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model

Yield parameters and water productivity of tropical and overseeded winter forages

Intensive pasture exploitation with tropical forages is one of the most efficient forms ruminant production. However, the results depends on the knowledge about these forages and the experience in its management. The objective of this work was to evaluate water productivity (WP) and yield parameters of Megathyrsus maximum cv. Mombaça “Guinea grass” and Cynodon spp. “Bermuda grass”, in single culture and overseeded with oats + ryegrass in autumn/winter period. Irrigation management was done considering a depletion factor of 0.3 (70% of the humidity the field capacity). The experimental design was a randomized complete block design with four replications, in which the forages are the treatments with evaluation at the time of the cycles. For all evaluated parameters, the best results were obtained with the Guinea grass in exclusive cultivation. The TFP (Total Forage Productivity) of Guinea grass was superior to Bermuda grass (59.3 and 30.2 Mg ha-1, respectively), in accordance with its average LAI (Leaf area index) values of 4.8 and 2.5, respectively. The most efficient use of water occurred for Guinea grass in exclusive cultivation, and the intercropped crops did not present increases in the WP. In the autumn/winter period, the accumulated TFP of the exclusive crop of Guinea grass was higher than the other crops. However, the SDM of Bermuda grass was higher than the other crops. In winter, the highest LAI occurred in Guinea grass in exclusive cultivation, followed by the overseeded Guinea grass, 4.8 and 4.5, respectively. Consortia did not increase forage yield during the winter. The consortiums did not increase forage yield during the winter

Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential

Background Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature

The inventory of geological heritage of the state of São Paulo, Brazil: Methodological basis, results and perspectives

An inventory of geological sites based on solid and clear criteria is a first step for any geoconservation strategy. This paper describes the method used in the geoheritage inventory of the State of São Paulo, Brazil, and presents its main results. This inventory developed by the geoscientific community aimed to identify geosites with scientific value in the whole state, using a systematic approach. All 142 geosites representative of 11 geological frameworks were characterised and quantitatively evaluated according to their scientific value and risk of degradation, in order to establish priorities for their future management. An online database of the inventory is under construction, which will be available to be easily consulted and updated by the geoscientific community. All data were made available to the State Geological Institute as the backbone for the implementation of a future state geoconservation strategy.The authors acknowledge the Science Without Borders Programme, Process 075/2012, which supported this study and the São Paulo Research Foundation (FAPESP), Process 2011/17261-6. We also thanks C. Mazoca for his help with maps and figures.info:eu-repo/semantics/acceptedVersio