Isotope ratio mass spectrometry (IRMS) methods for distinguishing organic from conventional food products: a review

The use of isotopic analytical methods for food authentication was established years ago. Changes in food technology and consumer behavior, as well as the increasing number of cases of food fraud, necessitate ongoing research for reliable analytical authentication techniques. This literature review examines recent applications of stable isotope ratio analysis that can be used in cases of organic food mislabeling. Different isotope ratio mass spectrometry (IRMS) techniques are described in this article, including bulk IRMS analysis and the combination of IRMS with novel sample preparation and compound extraction techniques. Compound-specific IRMS analysis comprising mainly hyphenated techniques, such as gas chromatography GC-IRMS, was also considered, and was found to frequently overcome the limitations exhibited by bulk analysis. A wide range of food product categories were covered, including cereals, vegetables, fruit, animal products, and seafood, while the importance of statistical analysis was underlined in determining which stable isotopic compositions (δ(15N), δ(34S), δ(18O), δ(13C), or δ(2H)) could be used as reliable organic authenticity marker

NMR profiling of grape musts from some Italian regions

With wine fraud, being a widespread problem [1], the need for more sophisticated and precise analytical methods of its detection remains ever persistent. Nuclear magnetic resonance (NMR) spectroscopy has been widely used for analysis of wine in recent years [2,3], but wine musts were much less studied; in fact, only one paper dealt with the NMR spectra of actual musts [4]. Difficulties arise mostly because grape musts are “live” objects, which undergo rapid fermentation at room temperature, if not inhibited either by freezing or chemical preservative; but even such measures are not sufficient to halt it completely [5]. We have investigated over 300 samples of grape must from 17 of 20 different Italian regions using 1H NMR spectroscopy with water signal suppression, postprocessing in the MatLab software with dynamic alignment [6] and optimized binning [7] to alleviate the effect of fermentation on the chemical shifts of mobile protons. After that, multivariate statistics was performed with techniques such as PCA, PLS-DA and OPLS-DA with respect to various group parameters such as regions, vitivinicultural zones, harvest periods and grape varieties. Advantages and drawbacks of each method were addresse

Elemental and isotopic profiling: a tool for distinguishing the botanical origin of oenological tannins

Much contemporary evidence underscores the pathophysiological importance of Ca2+ handling by acidic organelles such as lysosomes. Whereas our knowledge of how Ca2+ is released from these acidic Ca2+ stores (the ‘outs’) is advancing, we know relatively little about how Ca2+ uptake is effected (the ‘ins’). Here I highlight new work identifying animal Ca2+/H+ (CAX) exchangers that localize to acidic organelles, mediate Ca2+ uptake and regulate cell migration in vivo. Continued molecular definition of the acidic Ca2+ store toolkit provides new insight into Ca2+-dependent function

Noncooperative and Cooperative Transmission Schemes with Precoding and Beamforming

The next generation mobile networks are expected to provide multimedia applications with a high quality of service. On the other hand, interference among multiple base stations (BS) that co-exist in the same location limits the capacity of wireless networks. In conventional wireless networks, the base stations do not cooperate with each other. The BSs transmit individually to their respective mobile stations (MS) and treat the transmission from other BSs as interference. An alternative to this structure is a network cooperation structure. Here, BSs cooperate with other BSs to simultaneously transmit to their respective MSs using the same frequency band at a given time slot. By doing this, we significantly increase the capacity of the networks. This thesis presents novel research results on a noncooperative transmission scheme and a cooperative transmission scheme for multi-user multiple-input-multiple-output orthogonal frequency division multiplexing (MIMO-OFDM). We first consider the performance limit of a noncooperative transmission scheme. Here, we propose a method to reduce the interference and increase the throughput of orthogonal frequency division multiplexing (OFDM) systems in co-working wireless local area networks (WLANs) by using joint adaptive multiple antennas(AMA) and adaptive modulation (AM) with acknowledgement (ACK) Eigen-steering. The calculation of AMA and AM are performed at the receiver. The AMA is used to suppress interference and to maximize the signal-to-interference-plus-noise ratio (SINR). The AM scheme is used to allocate OFDM sub-carriers, power, and modulation mode subject to the constraints of power, discrete modulation, and the bit error rate (BER). The transmit weights, the allocation of power, and the allocation of sub-carriers are obtained at the transmitter using ACK Eigen-steering. The derivations of AMA, AM, and ACK Eigen-steering are shown. The performance of joint AMA and AM for various AMA configurations is evaluated through the simulations of BER and spectral efficiency (SE) against SIR. To improve the performance of the system further, we propose a practical cooperative transmission scheme to mitigate against the interference in co-working WLANs. Here, we consider a network coordination among BSs. We employ Tomlinson Harashima precoding (THP), joint transmit-receive beamforming based on SINR (signal-to-interference-plus-noise-ratio) maximization, and an adaptive precoding order to eliminate co-working interference and achieve bit error rate (BER) fairness among different users. We also consider the design of the system when partial channel state information (CSI) (where each user only knows its own CSI) and full CSI (where each user knows CSI of all users) are available at the receiver respectively. We prove analytically and by simulation that the performance of our proposed scheme will not be degraded under partial CSI. The simulation results show that the proposed scheme considerably outperforms both the existing noncooperative and cooperative transmission schemes. A method to design a spectrally efficient cooperative downlink transmission scheme employing precoding and beamforming is also proposed. The algorithm eliminates the interference and achieves symbol error rate (SER) fairness among different users. To eliminate the interference, Tomlinson Harashima precoding (THP) is used to cancel part of the interference while the transmit-receive antenna weights cancel the remaining one. A new novel iterative method is applied to generate the transmit-receive antenna weights. To achieve SER fairness among different users and further improve the performance of MIMO systems, we develop algorithms that provide equal SINR across all users and order the users so that the minimum SINR for each user is maximized. The simulation results show that the proposed scheme considerably outperforms existing cooperative transmission schemes in terms of the SER performance and complexity and approaches an interference free performance under the same configuration. We could improve the performance of the proposed interference cancellation further. This is because the proposed interference cancellation does not consider receiver noise when calculating the transmit-receive weight antennas. In addition, the proposed scheme mentioned above is designed specifically for a single-stream multi-user transmission. Here, we employ THP precoding and an iterative method based on the uplink-downlink duality principle to generate the transmit-receive antenna weights. The algorithm provides an equal SINR across all users. A simpler method is then proposed by trading off the complexity with a slight performance degradation. The proposed methods are extended to also work when the receiver does not have complete Channel State Informations (CSIs). A new method of setting the user precoding order, which has a much lower complexity than the VBLAST type ordering scheme but with almost the same performance, is also proposed. The simulation results show that the proposed schemes considerably outperform existing cooperative transmission schemes in terms of SER performance and approach an interference free performance. In all the cooperative transmission schemes proposed above, we use THP to cancel part of the interference. In this thesis, we also consider an alternative approach that bypasses the use of THP. The task of cancelling the interference from other users now lies solely within the transmit-receive antenna weights. We consider multiuser Gaussian broadcast channels with multiple antennas at both transmitter and receivers. An iterative multiple beamforming (IMB) algorithm is proposed, which is flexible in the antenna configuration and performs well in low to moderate data rates. Its capacity and bit error rate performance are compared with the ones achieved by the traditional zero-forcing method

Multidimensional natal isotopic niches refect migratory patterns in birds

7openInternationalBothNaturally occurring stable isotope ratios in animal tissues allow estimation of species trophic position and ecological niche. Measuring multiple isotopes of migratory species along flyway bottlenecks offers the opportunity to sample multiple populations and species whose tissues carry information at continental scales. We measured δ2H, δ18O, δ13C, δ15N in juvenile feathers of 21 bird species captured at a migratory bottleneck in the Italian Alps. We examined if trends in individual isotopes reflected known migratory strategies and whether dietary (δ13C–δ15N) and spatially-explicit breeding origin (δ2H–δ18O) niche breadth (NB) differed among long-distance trans-Saharan (TS), short-distance (IP) and irruptive (IR) intra-Palearctic migrants, and whether they correlated with reported populations long-term trends. In both TS and IP groups, species δ2H declined with capture date, indicating that northern populations reached the stopover site later in the season, following a Type-I migration strategy. Values of δ2H indicated that breeding range of TS migrants extended farther north than IP and IR migrants. The breeding season was longer for IP migrants whose δ13C and δ15N values declined and increased, respectively, with time of capture. Average species dietary NB did not differ among migratory groups, but TS migrants displayed wider breeding origin niches, suggesting that long-distant migration is linked to broader ecological niches. Isotope origin NB well reflected species geographic range extent, while dietary NB did not correlate with literature accounts of species’ diet. We found no relationship between species breeding NB and population trends in Europe, suggesting that conditions in the breeding grounds, as inferred by stable isotopes, are not the only determinant of species’ long-term persistence. We demonstrate that ringing activities and isotopic measurements of passerines migrating through a bottleneck represents a unique opportunity to investigate large-scale life-history phenomena relevant to conservation.openFranzoi, A.; Larsen, S.; Franceschi, P.; Hobson, K. A.; Pedrini, P.; Camin, F.; Bontempo, L.Franzoi, A.; Larsen, S.; Franceschi, P.; Hobson, K.A.; Pedrini, P.; Camin, F.; Bontempo, L

Aromatic characterization of Moscato Giallo by GC-MS/MS and stable isotopic ratio analysis of the major volatile compounds

Among the Moscato grapes, Moscato Giallo is a winegrape variety characterized by a highcontent of free and glycosylated monoterpenoids, which gives very aromatic wines. Thearomatic bouquet of Moscato Giallo is strongly influenced by the high concentration of linalool, geraniol, linalool oxides, limonene, α-terpineol, citronellol, HO-trienol, HO-diols, 8-Hydroxylinalool, geranic acid and β-myrcene, that give citrus, rose, and peach notes. Except the quali-quantitative analysis, no investigations regarding the isotopic values of the target volatile compounds are documented in literature. Stable isotope ratio analysis represents a modern and powerful tool used by the laboratories responsible for official consumer protection, for the food quality and genuineness assessment. In this study, samples of Moscato Giallo were collected during the harvest season in 2019 from two Italian regions:Trentino – Alto Adige and Veneto, known lands for the cultivation of this aromatic variety. The flavor compounds were extracted from grapes and wines, after alcoholic fermentation of grape juice, and analysed by GC-MS/MS. The results confirmed the presence of typical terpenoids both in free and glycosylated form, responsible for the characteristic aroma of Moscato Giallo variety. The aromatic compounds were also analysed by GC-C\Py-IRMS for a preliminary investigation. The compound-specific isotope ratio analysis allowed to determine the carbon (δ13C) and hydrogen (δ2H) isotopic signatures of the major volatile compounds for the first time