Comparation of the new rebound tonometer IOPen and the Goldmann tonometer, and their relationship to corneal properties

Purpose To compare the intraocular pressures (IOPs) obtained with the IOPen rebound tonometer, Goldmann applanation tonometer (GAT) and the ocular response analyzer (ORA) and investigate the effects of corneal biomechanical properties on IOPen measurements. Methods A total of 198 normal eyes were included in this cross-sectional and randomized study. Three measurements were taken using IOPen. Agreement between tonometers was calculated using the Bland and Altman limits of agreement (LoA) analysis. Results The median IOPen IOP was 3mmHg below the GAT (Po0.001), 3mmHg below the ORA IOP similar to Goldmann (IOPg), and 3mmHg below the ORA IOP corrected using corneal parameters (IOPcc)(Po0.01). The LoA width between the IOPen and GAT IOPs varied between 13.92 (mean IOPen IOP) and 15.99mmHg (third IOPen measurement). The central corneal thickness (CCT) was unrelated to IOPen measurements (P40.05). Corneal hysteresis (CH) and corneal rigidity factor (CRF) were correlated with IOPen and GAT. Conclusions IOPen underestimated the IOP compared with GAT and ORA. The effect of measurement quality or measurement order on IOPen was low. CCT did not affect the IOPen, but the CH and CRF did. The LoA width between the IOPen and GAT IOPs was higher than between the ORA IOPg or ORA IOPcc and GAT IOPs

Acrysof® toric intraocular lens implantation in cataract surgery

Aim: To assess the medium term outcomes of Acrysof® toric intraocular lens implantation in 54 patients (54 eyes). Methods: Uncorrected visual acuity (UCVA), best corrected visual acuity (BCVA), preoperative astigmatism, residual postoperative astigmatism, and global average and model-specific intraocular lens (IOL) rotation grade were analyzed. Results: At 2-months of follow-up,the mean UCVA was 0.83 (SD: 0.14) Snellen scale, with 73.9% of the patients ≥0.8, and 32.6% with 1.0. Mean BCVA achieved was 0.94 (SD: 0.10). Mean preoperatory astigmatism was –2.25 diopters (D) (SD: 0.78), and mean postoperative astigmatism was -0.32 D (SD: 0.56), with significant differences between both groups (p<0.001). Model-specific mean residual astigmatism was –0.1 D for T3, –0.27 D for T4 and –0.43 D for T5, without significant differences between the three models (p=0.483). Mean IOL-axis rotation grade was 3.87±3.25 degrees, with 91.6% of implanted lens within 10° of predicted axis. Discussion: T3, T4 and T5 Acrysof® Toric intraocular lenses can correct preoperative astigmatism with a high success rate in terms of UCVA, and residual postoperative astigmatism, with minimum IOL-rotation grade at 2 months follow-up period

Intravitreal bevacizumab associated with grid laser photocoagulation in macular edema secondary to branch retinal vein occlusion

To evaluate intravitreal bevacizumab (IVB) combined with grid laser photocoagulation in macular edema (ME) secondary to branch retinal vein occlusion (BRVO). METHODS: Eight eyes (8 patients) with ME associated with BRVO with at least 3 months of evolution since symptom onset were included. All subjects underwent measurement of best-corrected visual acuity (BCVA) and imaging with spectral domain optical coherence tomography (SD-OCT) at baseline and 1, 3, 6, and 12 months. Intravitreal bevacizumab was administered at baseline and macular grid laser photocoagulation 1 month later. During follow-up, additional IVB was administered based on physician discretion if persistent or recurrent intraretinal fluid (cysts) was observed on SD-OCT. The mean BCVA and SD-OCT central subfield thickness (CST) values were determined at each time point. Fisher exact test was performed to assess differences between baseline and post-treatment BCVA and SD-OCT measurements. RESULTS: The mean baseline BCVA was 0.28+/-0.14 (mean+/-SD), and the mean CST was 479+/-137 microm. The mean BCVAs at 1, 3, 6, and 12 months were 0.47+/-0.18 (p=0.031), 0.56+/-0.50 (p=0.031), 0.65+/-0.60 (p=0.008), and 0.66+/-0.65 (p=0.016), respectively. The mean CST values at 1, 3, 6, and 12 months were 295+/-60 microm (p=0.008), 333+/-114 microm (p=0.070), 339+/-80 microm (p=0.008), and 335+/-109 microm (p=0.008). A mean 2.13 injections were administered; the second injection was administered a mean of 2.71 months after baseline. CONCLUSIONS: Combined treatment with IVB and macular grid photocoagulation provided good results and may be considered as an alternative therapy for ME in BRVO. Further studies are needed to assess these preliminary results

Towards the development of agent-based organizations through MDD

Electronic version of an article published as International Journal on Artificial Intelligence Tools, 22, 2, 2013, DOI 10.1142/S0218213013500024 © World Scientific Publishing Company http://www.worldscientific.com/worldscinet/ijaitVirtual Organizations are a mechanism where agents can demonstrate their social skills since they can work in a cooperative and collaborative way. Nonetheless, the development of organizations using Multi-Agent Systems (MAS) requires extensive experience in different methodologies and platforms. Model-Driven Development (MDD) is a technique for generating application code that is developed from basic models and meta-models using a variety of automatic transformations. This paper presents an approach to develop and deploy organization-oriented Multi-Agent Systems using a model-driven approach. Based on this idea, we introduce a relatively generic agent-based meta-model for a Virtual Organization, which was created by a comprehensive analysis of the organization-oriented methodologies used in MAS. Following the MDD approach, the concepts and relationships obtained were mapped into two different platforms available for MAS development, allowing the validation of our proposal. In this way, the resultant approach can generate Virtual Organization deployments from unified meta-models, facilitating the development process of agent-based software from the user point of view.This work was partially supported by TIN2009-13839-C03-01 and PROMETEO/2008/051 projects of the Spanish government and CONSOLIDER-INGENIO 2010 under grant CSD2007-00022.Agüero, J.; Carrascosa Casamayor, C.; Rebollo Pedruelo, M.; Julian Inglada, VJ. (2013). Towards the development of agent-based organizations through MDD. International Journal on Artificial Intelligence Tools. 22(2):1-34. https://doi.org/10.1142/S0218213013500024S134222Argente, E., Julian, V., & Botti, V. (2006). Multi-Agent System Development Based on Organizations. Electronic Notes in Theoretical Computer Science, 150(3), 55-71. doi:10.1016/j.entcs.2006.03.005Bézivin, J. (2005). On the unification power of models. Software & Systems Modeling, 4(2), 171-188. doi:10.1007/s10270-005-0079-0Bresciani, P., Perini, A., Giorgini, P., Giunchiglia, F., & Mylopoulos, J. (2004). Tropos: An Agent-Oriented Software Development Methodology. Autonomous Agents and Multi-Agent Systems, 8(3), 203-236. doi:10.1023/b:agnt.0000018806.20944.efFoster, I., Kesselman, C., & Tuecke, S. (2001). The Anatomy of the Grid: Enabling Scalable Virtual Organizations. The International Journal of High Performance Computing Applications, 15(3), 200-222. doi:10.1177/109434200101500302Hahn, C., Madrigal-Mora, C., & Fischer, K. (2008). A platform-independent metamodel for multiagent systems. Autonomous Agents and Multi-Agent Systems, 18(2), 239-266. doi:10.1007/s10458-008-9042-0HORLING, B., & LESSER, V. (2004). A survey of multi-agent organizational paradigms. The Knowledge Engineering Review, 19(4), 281-316. doi:10.1017/s0269888905000317Huhns, M. N., & Singh, M. P. (2005). Service-oriented computing: key concepts and principles. IEEE Internet Computing, 9(1), 75-81. doi:10.1109/mic.2005.21Huhns, M. N., Singh, M. P., Burstein, M., Decker, K., Durfee, E., Finin, T., … Zavala, L. (2005). Research Directions for Service-Oriented Multiagent Systems. IEEE Internet Computing, 9(6), 65-70. doi:10.1109/mic.2005.132Kolp, M., Giorgini, P., & Mylopoulos, J. (2006). Multi-Agent Architectures as Organizational Structures. Autonomous Agents and Multi-Agent Systems, 13(1), 3-25. doi:10.1007/s10458-006-5717-6OHTANI, T., CASE, S., AZARMI, N., & THINT, M. (2002). AN INTELLIGENT SYSTEM FOR MANAGING AND UTILIZING INFORMATION RESOURCES OVER THE INTERNET. International Journal on Artificial Intelligence Tools, 11(01), 117-138. doi:10.1142/s0218213002000800Omicini, A., Ricci, A., & Viroli, M. (2005). RBAC for Organisation and Security in an Agent Coordination Infrastructure. Electronic Notes in Theoretical Computer Science, 128(5), 65-85. doi:10.1016/j.entcs.2004.11.045Papazoglou, M. P., & Georgakopoulos, D. (2003). Introduction. Communications of the ACM, 46(10), 24. doi:10.1145/944217.944233Papazoglou, M. P., Traverso, P., Dustdar, S., & Leymann, F. (2007). Service-Oriented Computing: State of the Art and Research Challenges. Computer, 40(11), 38-45. doi:10.1109/mc.2007.400Selic, B. (2003). The pragmatics of model-driven development. IEEE Software, 20(5), 19-25. doi:10.1109/ms.2003.1231146SKARMEAS, N. P., & CLARK, K. L. (2002). COMPONENT BASED AGENT CONSTRUCTION. International Journal on Artificial Intelligence Tools, 11(01), 139-163. doi:10.1142/s0218213002000812Zambonelli, F., Jennings, N. R., & Wooldridge, M. (2003). Developing multiagent systems. ACM Transactions on Software Engineering and Methodology, 12(3), 317-370. doi:10.1145/958961.95896

Antimycobacterial drug discovery using Mycobacteria-infected amoebae identifies anti-infectives and new molecular targets

Tuberculosis remains a serious threat to human health world-wide, and improved efficiency of medical treatment requires a better understanding of the pathogenesis and the discovery of new drugs. In the present study, we performed a whole-cell based screen in order to complete the characterization of 168 compounds from the GlaxoSmithKline TB-set. We have established and utilized novel previously unexplored host-model systems to characterize the GSK compounds, i.e. the amoeboid organisms D. discoideum and A. castellanii, as well as a microglial phagocytic cell line, BV2. We infected these host cells with Mycobacterium marinum to monitor and characterize the anti-infective activity of the compounds with quantitative fluorescence measurements and high-content microscopy. In summary, 88.1% of the compounds were confirmed as antibiotics against M. marinum, 11.3% and 4.8% displayed strong anti-infective activity in, respectively, the mammalian and protozoan infection models. Additionally, in the two systems, 13-14% of the compounds displayed pro-infective activity. Our studies underline the relevance of using evolutionarily distant pathogen and host models in order to reveal conserved mechanisms of virulence and defence, respectively, which are potential "universal" targets for intervention. Subsequent mechanism of action studies based on generation of over-expresser M. bovis BCG strains, generation of spontaneous resistant mutants and whole genome sequencing revealed four new molecular targets, including FbpA, MurC, MmpL3 and GlpK

Genomics-assisted breeding in four major pulse crops of developing countries: present status and prospects

The global population is continuously increasing and is expected to reach nine billion by 2050. This huge population pressure will lead to severe shortage of food, natural resources and arable land. Such an alarming situation is most likely to arise in developing countries due to increase in the proportion of people suffering from protein and micronutrient malnutrition. Pulses being a primary and affordable source of proteins and minerals play a key role in alleviating the protein calorie malnutrition, micronutrient deficiencies and other undernourishment-related issues. Additionally, pulses are a vital source of livelihood generation for millions of resource-poor farmers practising agriculture in the semi-arid and sub-tropical regions. Limited success achieved through conventional breeding so far in most of the pulse crops will not be enough to feed the ever increasing population. In this context, genomics-assisted breeding (GAB) holds promise in enhancing the genetic gains. Though pulses have long been considered as orphan crops, recent advances in the area of pulse genomics are noteworthy, e.g. discovery of genome-wide genetic markers, high-throughput genotyping and sequencing platforms, high-density genetic linkage/QTL maps and, more importantly, the availability of whole-genome sequence. With genome sequence in hand, there is a great scope to apply genome-wide methods for trait mapping using association studies and to choose desirable genotypes via genomic selection. It is anticipated that GAB will speed up the progress of genetic improvement of pulses, leading to the rapid development of cultivars with higher yield, enhanced stress tolerance and wider adaptability