Suivi photométrique de candidates exoplanètes identifiées par le Transiting Exoplanet Survey Satellite

La majorité des exoplanètes connues à ce jour ont été découvertes par la méthode du transit, qui infère indirectement l’existence de tels objets, si l’alignement le permet, en mesurant la baisse temporaire et répétée de la brillance d’une étoile lors du passage d’une exoplanète devant celle-ci. La recherche de biosignatures, donc de vie, dans l’atmosphère d’une exoplanète est désormais le principal objectif dans ce domaine d’études, et pour maintes raisons, celles de taille de moins d’approximativement deux rayons terrestres autour d’étoiles naines rouges sont particulièrement convoitées. Afin de connaître davantage de tels systèmes dans le voisinage solaire et dans toutes les régions du ciel, le Transiting Exoplanet Survey Satellite (TESS) fut lancé en avril 2018. Le grand échantillonnage de 21'' par pixel des caméras à bord de TESS résulte fréquemment à une contamination des données des étoiles d’intérêt montrant un signal prometteur de transit, par le flux d’autres étoiles à proximité. Lorsque l’une de ces sources contaminantes est une étoile binaire à éclipses, phénomène astrophysique pouvant mimer un transit, la détection constitue très souvent un événement faux positif. Ainsi, de nouvelles observations photométriques et spectroscopiques sont généralement requises pour identifier les véritables exoplanètes. Ce mémoire présente les résultats du suivi photométrique de neuf candidates exoplanètes identifiées par TESS à l’Observatoire du Mont-Mégantic avec la caméra Planètes Extra-Solaires en Transit et Occultations (PESTO). Une routine d’ajustement de courbe de transit développée durant cette maîtrise procure une estimation de certains paramètres physiques (rayons, demi-grand axe et inclinaison) des candidates. Parmi celles-ci, TOI 1452.01 ressort du lot, car cette probable exoplanète d’environ deux rayons terrestres orbite dans la zone habitable de son hôte naine rouge, c’est-à-dire à une distance permettant la présence d’eau liquide à sa surface.The majority of the exoplanets known to date have been discovered using the transit method, which indirectly infers the existence of such objects by measuring a temporary and repeated drop in the brightness of a star when, for the right alignement, an exoplanet passes in front of it. The search for biosignatures, thus life, in an exoplanet atmosphere is now the main objective in this field of study, and for several reasons, planets with a radius less than approximately two Earth radii around red dwarfs are particularly targeted. With the goal of finding more such systems in the solar neighbourhood and in all regions of the sky, the Transiting Exoplanet Survey Satellite (TESS) was launched in April 2018. The large image sampling of 21'' per pixel of the cameras on board TESS often results in data contamination of stars showing promising transit signal, by the flux of nearby stars. If one of these contaminating sources happens to be an eclipsing binary, an astrophysical phenomenon able to mimic a transit, the detection is most likely a false positive event. Thus, follow-up observations in photometry and in spectroscopy are generally required to identify the genuine exoplanets. This thesis presents the results of a photometric monitoring campaign at the Observatoire du Mont-Mégantic with the Planètes Extra-Solaires en Transit et Occultations (PESTO) camera of nine exoplanet candidates identified by TESS. A transit curve fitting routine developed during this master’s provides an estimation for certain physical parameters (radius, semi-major axis and inclination) of these candidates. Among them, TOI 1452.01 stands out, because this probable exoplanet has an estimated radius close to two Earth radii, in addition to being located within the habitable zone of its red dwarf host, i.e. at a distance allowing the presence of liquid water on its surface

Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice

<p>Abstract</p> <p>Background</p> <p><it>Lats1 </it>(large tumor suppressor 1) codes for a serine/threonine kinase that plays a role in the progression through mitosis. Genetic studies demonstrated that the loss of LATS1 in mouse, and of its ortholog <it>wts </it>(warts) in Drosophila, is associated with increased cancer incidence. There are conflicting reports, however, as to whether overexpression of <it>Lats1 </it>inhibits cell proliferation. CUX1 is a transcription factor that exists in different isoforms as a result of proteolytic processing or alternative transcription initiation. Expression of p110 and p75 CUX1 in transgenic mice increases the susceptibility to cancer in various organs and tissues. In tissue culture, p110 CUX1 was shown to accelerate entry into S phase and stimulate cell proliferation.</p> <p>Results</p> <p>Genome-wide location arrays in cell lines of various cell types revealed that <it>Lats1 </it>was a transcriptional target of CUX1. Scanning ChIP analysis confirmed that CUX1 binds to the immediate promoter of <it>Lats1</it>. Expression of <it>Lats1 </it>was reduced in cux1^-/-MEFs, whereas it was increased in cells stably or transiently expressing p110 or p75 CUX1. Reporter assays confirmed that the immediate promoter of <it>Lats1 </it>was sufficient to confer transcriptional activation by CUX1. <it>Lats1 </it>was found to be overexpressed in tumors from the mammary gland, uterus and spleen that arise in p110 or p75 CUX1 transgenic mice. In tissue culture, such elevated LATS1 expression did not hinder cell cycle progression in cells overexpressing p110 CUX1.</p> <p>Conclusion</p> <p>While inactivation of <it>Lats1</it>/<it>wts </it>in mouse and Drosophila can increase cancer incidence, results from the present study demonstrate that <it>Lats1 </it>is a transcriptional target of CUX1 that can be overexpressed in tumors of various tissue-types. Interestingly, two other studies documented the overexpression of <it>LATS1 </it>in human cervical cancers and basal-like breast cancers. We conclude that, similarly to other genes involved in mitotic checkpoint, cancer can be associated with either loss-of-function or overexpression of <it>Lats1</it>.</p

TOI-1452 b: SPIRou and TESS Reveal a Super-Earth in a Temperate Orbit Transiting an M4 Dwarf

Exploring the properties of exoplanets near or inside the radius valley provides insight on the transition from the rocky super-Earths to the larger, hydrogen-rich atmosphere mini-Neptunes. Here, we report the discovery of TOI-1452b, a transiting super-Earth (R-p = 1.67 +/- 0.07 R-circle times) in an 11.1 day temperate orbit (T-eq = 326 +/- 7 K) around the primary member (H = 10.0, T-eff = 3185 +/- 50 K) of a nearby visual-binary M dwarf. The transits were first detected by the Transiting Exoplanet Survey Satellite, then successfully isolated between the two 3.'' 2 companions with ground-based photometry from the Observatoire du Mont-Megantic and MuSCAT3. The planetary nature of TOI-1452b was established through high-precision velocimetry with the near-infrared SPTRou spectropolarimeter as part of the ongoing SPIRou Legacy Survey. The measured planetary mass (4.8 +/- 1.3 M-circle times) and inferred bulk density (5.6(-)(1.)(6)(+1.8) g cm(-3)) is suggestive of a rocky core surrounded by a volatile-rich envelope. More quantitatively, the mass and radius of TOI-1452b, combined with the stellar abundance of refractory elements (Fe, Mg, and Si) measured by SPTRou, is consistent with a core-mass fraction of 18% +/- 6% and a water-mass fraction of 22(-13)(+21)%. The water world candidate TOI-14521) is a prime target for future atmospheric characterization with JWST, featuring a transmission spectroscopy metric similar to other well-known temperate small planets such as LHS 1140b and K2-18 b. The system is located near Webb's northern continuous viewing zone, implying that is can be followed at almost any moment of the year

APERO: A PipelinE to Reduce Observations -- Demonstration with SPIRou

With the maturation of near-infrared high-resolution spectroscopy, especially when used for precision radial velocity, data reduction has faced unprecedented challenges in terms of how one goes from raw data to calibrated, extracted, and corrected data with required precisions of thousandths of a pixel. Here we present APERO (A PipelinE to Reduce Observations), specifically focused on SPIRou, the near-infrared spectropolarimeter on the Canada--France--Hawaii Telescope (SPectropolarim\`etre InfraROUge, CFHT). In this paper, we give an overview of APERO and detail the reduction procedure for SPIRou. APERO delivers telluric-corrected 2D and 1D spectra as well as polarimetry products. APERO enables precise stable radial velocity measurements on sky (via the LBL algorithm), good to at least ~2 m/s over the current 5-year lifetime of SPIRou.Comment: Accepted for publication in PASP. 55 pages, 29 figures, 10 pages of Appendice

CUX1 transcription factor is required for optimal ATM/ATR-mediated responses to DNA damage

The p110 Cut homeobox 1 (CUX1) transcription factor regulates genes involved in DNA replication and chromosome segregation. Using a genome-wide-approach, we now demonstrate that CUX1 also modulates the constitutive expression of DNA damage response genes, including ones encoding ATM and ATR, as well as proteins involved in DNA damage-induced activation of, and signaling through, these kinases. Consistently, RNAi knockdown or genetic inactivation of CUX1 reduced ATM/ATR expression and negatively impacted hallmark protective responses mediated by ATM and ATR following exposure to ionizing radiation (IR) and UV, respectively. Specifically, abrogation of CUX1 strongly reduced ATM autophosphorylation after IR, in turn causing substantial decreases in (i) levels of phospho-Chk2 and p53, (ii) γ-H2AX and Rad51 DNA damage foci and (iii) the efficiency of DNA strand break repair. Similarly remarkable reductions in ATR-dependent responses, including phosphorylation of Chk1 and H2AX, were observed post-UV. Finally, multiple cell cycle checkpoints and clonogenic survival were compromised in CUX1 knockdown cells. Our results indicate that CUX1 regulates a transcriptional program that is necessary to mount an efficient response to mutagenic insult. Thus, CUX1 ensures not only the proper duplication and segregation of the genetic material, but also the preservation of its integrity

Comprehensive High-resolution Chemical Spectroscopy of Barnard's Star with SPIRou

Determination of fundamental parameters of stars impacts all fields of astrophysics, from galaxy evolution to constraining the internal structure of exoplanets. This paper presents a detailed spectroscopic analysis of Barnard's star that compares an exceptionally high-quality (signal-to-noise ratio of $>$2500 in the $H$ band), high-resolution NIR spectrum taken with CFHT/SPIRou to PHOENIX-ACES stellar atmosphere models. The observed spectrum shows thousands of lines not identified in the models with a similar large number of lines present in the model but not in the observed data. We also identify several other caveats such as continuum mismatch, unresolved contamination and spectral lines significantly shifted from their expected wavelengths, all of these can be a source of bias for abundance determination. Out of $>10^4$ observed lines in the NIR that could be used for chemical spectroscopy, we identify a short list of a few hundred lines that are reliable. We present a novel method for determining the effective temperature and overall metallicity of slowly-rotating M dwarfs that uses several groups of lines as opposed to bulk spectral fitting methods. With this method, we infer $T_{\rm eff}$ = 3231 $\pm$ 21 K for Barnard's star, consistent with the value of 3238 $\pm$ 11 K inferred from the interferometric method. We also provide abundance measurements of 15 different elements for Barnard's star, including the abundances of four elements (K, O, Y, Th) never reported before for this star. This work emphasizes the need to improve current atmosphere models to fully exploit the NIR domain for chemical spectroscopy analysis.Comment: 24 pages, 18 figures, submitted to Ap

Revisiting radial velocity measurements of the K2-18 system with the line-by-line framework

The cross-correlation function and template matching techniques have dominated the world of precision radial velocities for many years. Recently, a new technique, named line-by-line, has been developed as an outlier resistant way to efficiently extract radial velocity content from high resolution spectra. We apply this new method to archival HARPS and CARMENES data sets of the K2-18 system. After reprocessing the HARPS data set with the line-by-line framework, we are able to replicate the findings of previous studies. Furthermore, by splitting the full wavelength range into sub-domains, we were able to identify a systematic chromatic correlation of the radial velocities in the reprocessed CARMENES data set. After post-processing the radial velocities to remove this correlation, as well as rejecting some outlier nights, we robustly uncover the signal of both K2-18 b and K2-18 c, with masses that agree with those found from our analysis of the HARPS data set. We then combine both the HARPS and CARMENES velocities to refine the parameters of both planets, notably resulting in a revised mass and period for K2-18 c of 6.99+0.96−0.99 M⊕ and 9.2072 ± 0.0065 d, respectively. Our work thoroughly demonstrates the power of the line-by-line technique for the extraction of precision radial velocity information. © 2022 The Author(s) Published by Oxford University Press on behalf of Royal Astronomical Society.MR would like to acknowledge funding from the National Sciences and Research Council of Canada (NSERC), the Fonds de Recherche du Québec - Nature et Technologies (FRQNT), and the Institut de Recherche sur les Exoplanétes (iREx) for support towards his doctoral studies. PJA, JAC, and IR acknowledge financial support from the Spanish Agencia Estatal de Investigación of the Ministerio de Ciencia e Innovación (AEI-MCIN) and the European FEDER/ERF funds through projects PGC2018-098153-B-C33, PID2019-109522GB-C51/52/53/54, and the Centre of Excellence ‘Severo Ochoa’ and ‘María de Maeztu’ awards to the Instituto de Astrofísica de Andalucía (SEV-2017-0709), Centro de Astrobiología (MDM-2017-0737), and the Institut de Ciències de l’Espai (CEX2020-001058-M).Peer reviewe

CO or no CO? Narrowing the CO abundance constraint and recovering the H2O detection in the atmosphere of WASP-127 b using SPIRou

Precise measurements of chemical abundances in planetary atmospheres are necessary to constrain the formation histories of exoplanets. A recent study of WASP-127b, a close-in puffy sub-Saturn orbiting its solar-type host star in 4.2 d, using HST and Spitzer revealed a feature-rich transmission spectrum with strong excess absorption at 4.5 um. However, the limited spectral resolution and coverage of these instruments could not distinguish between CO and/or CO2 absorption causing this signal, with both low and high C/O ratio scenarios being possible. Here we present near-infrared (0.9--2.5 um) transit observations of WASP-127 b using the high-resolution SPIRou spectrograph, with the goal to disentangle CO from CO2 through the 2.3 um CO band. With SPIRou, we detect H2O at a t-test significance of 5.3 sigma and observe a tentative (3 sigma) signal consistent with OH absorption. From a joint SPIRou + HST + Spitzer retrieval analysis, we rule out a CO-rich scenario by placing an upper limit on the CO abundance of log10[CO]<-4.0, and estimate a log10[CO2] of -3.7^(+0.8)_(-0.6), which is the level needed to match the excess absorption seen at 4.5um. We also set abundance constraints on other major C-, O-, and N-bearing molecules, with our results favoring low C/O (0.10^(+0.10)_(-0.06)), disequilibrium chemistry scenarios. We further discuss the implications of our results in the context of planet formation. Additional observations at high and low-resolution will be needed to confirm these results and better our understanding of this unusual world.Comment: 23 pages, 13 figures, Submitted for publication in the Monthly Notice of the Royal Astronomical Societ

Training family physicians and residents in family medicine in shared decision making to improve clinical decisions regarding the use of antibiotics for acute respiratory infections: protocol for a clustered randomized controlled trial

<p>Abstract</p> <p>Background</p> <p>To explore ways to reduce the overuse of antibiotics for acute respiratory infections (ARIs), we conducted a pilot clustered randomized controlled trial (RCT) to evaluate DECISION+, a training program in shared decision making (SDM) for family physicians (FPs). This pilot project demonstrated the feasibility of conducting a large clustered RCT and showed that DECISION+ reduced the proportion of patients who decided to use antibiotics immediately after consulting their physician. Consequently, the objective of this study is to evaluate, in patients consulting for ARIs, if exposure of physicians to a modified version of DECISION+, DECISION+2, would reduce the proportion of patients who decide to use antibiotics immediately after consulting their physician.</p> <p>Methods/design</p> <p>The study is a multi-center, two-arm, parallel clustered RCT. The 12 family practice teaching units (FPTUs) in the network of the Department of Family Medicine and Emergency Medicine of Université Laval will be randomized to a DECISION+2 intervention group (experimental group) or to a no-intervention control group. These FPTUs will recruit patients consulting family physicians and residents in family medicine enrolled in the study. There will be two data collection periods: pre-intervention (baseline) including 175 patients with ARIs in each study arm, and post-intervention including 175 patients with ARIs in each study arm (total n = 700). The primary outcome will be the proportion of patients reporting a decision to use antibiotics immediately after consulting their physician. Secondary outcome measures include: 1) physicians and patients' decisional conflict; 2) the agreement between the parties' decisional conflict scores; and 3) perception of patients and physicians that SDM occurred. Also in patients, at 2 weeks follow-up, adherence to the decision, consultation for the same reason, decisional regret, and quality of life will be assessed. Finally, in both patients and physicians, intention to engage in SDM in future clinical encounters will be assessed. Intention-to-treat analyses will be applied and account for the nested design of the trial will be taken into consideration.</p> <p>Discussion</p> <p>DECISION+2 has the potential to reduce antibiotics use for ARIs by priming physicians and patients to share decisional process and empowering patients to make informed, value-based decisions.</p> <p>Trial Registration</p> <p>ClinicalTrials.gov: <a href="NCT01116076">NCT01116076</a></p

Vetting of 384 TESS Objects of Interest with TRICERATOPS and Statistical Validation of 12 Planet Candidates

We present TRICERATOPS, a new Bayesian tool that can be used to vet and validate TESS Objects of Interest (TOIs). We test the tool on 68 TOIs that have been previously confirmed as planets or rejected as astrophysical false positives. By looking in the false positive probability (FPP) -- nearby false positive probability (NFPP) plane, we define criteria that TOIs must meet to be classified as validated planets (FPP < 0.015 and NFPP < 10^-3), likely planets (FPP 10^-1). We apply this procedure on 384 unclassified TOIs and statistically validate 12, classify 125 as likely planets, and classify 52 as likely nearby false positives. Of the 12 statistically validated planets, 9 are newly validated. TRICERATOPS is currently the only TESS vetting and validation tool that models transits from nearby contaminant stars in addition to the target star. We therefore encourage use of this tool to prioritize follow-up observations that confirm bona fide planets and identify false positives originating from nearby stars.Comment: Accepted to A