Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy

Determination of βS haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil

βS haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio

Occurrence of insects and use of natural and synthetic insecticides in the storage of seeds of wild radish<br>Ocorrência de insetos e uso de inseticidas naturais e sintéticos no armazenamento de sementes de nabo forrageiro

Knowing the importance of preventive use of insecticides for seed treatment, this study aimed to identify potential insect pests of storage for seeds of radish by means of traps set on the field for six months and to evaluate the effect of natural and synthetic substances insecticides on the emergence of radish seedlings stored for 120 days. The bioassay, field-level, was conducted over a period of six months in the following environments: savannah, area of cultivation of crops, pasture and forest at the Federal Institute Goiano. For each of these environments were placed five traps made of PET bottles, containing respectively in maize, millet, soybean, crambe and wild radish. The traps were inspected every seven days to verify the the occurrence of insects plague. The substances were: 1) saffron, 2) limestone, 3) Gray, 4) neem, 5) diatomaceous earth, 6) chlorpyrifos, 7) deltamethrin and 8) control treatment. The experimental design was randomized blocks in factorial scheme 8 x 2 (8 substances and two storage periods, is not stored seeds and seeds stored for 120 days) with four replications. Were evaluated the percentage of emergence, hold rate of emergence, time to reach 50% emergence and the record of the dry mass of seedlings. The natural and synthetic substances do not interfere with seedling emergence in seeds of wild radish, even after storage for 120 days in a room. In the traps in the field, containing seeds of wild radish noted the presence of the insect Carpophilus sp.<p><p> Sabendo da importância do uso preventivo de inseticidas no tratamento de sementes, esta pesquisa teve por objetivos identificar possíveis insetos praga de armazenamento para sementes de nabo forrageiro por meio de armadilhas montadas em campo durante seis meses e avaliar o efeito de substâncias inseticidas, naturais e sintéticas na emergência de plântulas em sementes de nabo forrageiro armazenadas por 120 dias. O bioensaio, em nível de campo, foi conduzido por um período de seis meses nos seguintes ambientes: cerrado, área de cultivo de grandes culturas, pastagem e mata do Instituto Federal Goiano. Para cada um desses ambientes foram colocadas cinco armadilhas confeccionadas em garrafas PET, contendo respectivamente sementes de milho, milheto, soja, crambe e nabo forrageiro. As armadilhas foram inspecionadas a cada sete dias para a verificação da ocorrência de insetos praga. As substâncias consistiram de: 1) açafrão, 2) calcário, 3) cinza, 4) nim, 5) terra de diatomáceas, 6) clorpirifós, 7) deltametrina e 8) tratamento controle. O delineamento experimental foi em blocos ao acaso, no esquema fatorial 8 x 2, sendo oito substâncias e armazenamento por zero e 120 dias, com 4 repetições. Avaliaram-se porcentagem de emergência, índice de velocidade de emergência, tempo para atingir 50% da emergência e massa seca das plântulas emergidas. As substâncias naturais e sintéticas não interferem na emergência de plântulas em sementes de nabo forrageiro, mesmo após o armazenamento de 120 dias em sala climatizada. Nas armadilhas em campo contendo sementes de nabo forrageiro, foi observado a presença do inseto Carpophilus sp

Storage crambe seed treated with insecticides

O uso de sementes de baixa qualidade, com um menor potencial fisiológico, reflete numa das principais causas da baixa produtividade. Sendo assim as condições de armazenamento de sementes devem ser levadas em consideração. Esta pesquisa teve por objetivo avaliar a influência de inseticidas naturais e sintéticos na emergência e no armazenamento de sementes de crambe, já que tais sustâncias são indispensáveis para evitar a infestação das sementes de outras espécies por organismos nocivos. O delineamento experimental foi em blocos ao acaso, no esquema fatorial 8 x 3 (8 substâncias e 3 tempos de armazenamento), com 4 repetições. Avaliaram-se os seguintes inseticidas naturais: açafrão, calcário, cinza, nim, terra de diatomáceas; e sintéticos: clorpirifós e deltametrina; além do tratamento controle, constituído de sementes sem qualquer tipo de tratamento. Avaliaram-se a porcentagem de emergência, o índice de velocidade de emergência e tempo para atingir 50% da emergência. Em todas as características avaliadas, constatou-se nenhuma influência do nim sobre o vigor das sementes. Verificou-se desvantagens na aplicação dos inseticidas clorpirifós e terra de diatomáceas, que interferiram na velocidade de emergência das sementes de crambe. As sementes tratadas com os demais inseticidas tiveram comportamento diferenciado das sementes não tratadas após os 120 dias de armazenamento ao avaliar o tempo que as mesmas demoram a atingir 50% de emergência. The use of low quality seeds with a lower physiological reflects one of the major causes of low productivity. Thus the storage conditions of seed must be taken into consideration. This research aimed to evaluate the influence of natural and synthetic insecticides on emergence and seed storage of crambe, as these substances are essential to prevent infestation of seeds of other species by harmful organisms. The experimental design was a randomized block in factorial 3 x 8 ( 8 substances and 3 storage times ) with 4 replications. We assessed the following natural insecticides: saffron, lime, ash, neem, diatomaceous earth, and synthetic: chlorpyrifos and deltamethrin; besides the control consisting of seeds without any treatment. We evaluated the percentage of emergence, speed of emergence index and time to reach 50 % of emergency. In all characteristics, it was found that no influence of neem on seed vigor. There are disadvantages in the application of the insecticides chlorpyrifos and diatomaceous earth, which interfered with the emergence rate of seeds of crambe. The seeds treated with other insecticides had different behavior of untreated seeds after 120 days of storage to assess the time that they take to reach 50 % germination

Adesão aos Universitários aos Diversos Níveis de Atividade Física

Este estudo objetiva identificar os níveis de atividade física existentes na UCG e os fatores relacionados com a adesão dos universitários a essas atividades. O trabalho sugere a necessidade de um programa de promoção da atividade física para todos os estudantes, em especial para os de Zootecnia, com altos índices de sedentarismo, a fim de prevenir danos futuros à  saúde

Disclosing the Nutritional Quality Diversity of Portuguese Common Beans—The Missing Link for Their Effective Use in Protein Quality Breeding Programs

© 2021 by the authors.The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions’ nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap, a representative collection (106 accessions) was cropped under two contrasting environments (traditional versus heat stress) and evaluated in terms of nutritional quality by near-infrared spectroscopy. Protein quality was assessed, under the stressful environment, considering the individual amino acid contents and the activity of trypsin inhibitors through mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. On top of strong genotypic control, the nutritional composition (protein, fat, fiber, moisture and ash) was also highly influenced by the environment and by genotype × environment interaction, with a clear nutritional quality ranking change for the accessions in heat stress conditions. Classified into three clusters, the accessions from the cluster with the highest individual amino acid and protein contents also showed higher trypsin inhibitor activity (TIA). Since different levels of TIA had no translation into contrasting protein digestibility, breeders focusing on common beans’ protein quality improvement, especially under challenging warming climate conditions, may take advantage of this group of accessions.This research was funded by FCT, Portugal, through BEGEQA project (PTDC/AGRTEC/3555/2012), E.M. PhD fellowship (SFRH/BD/89287/2012 and R&D unit, UIDB/04551/2020 (GREEN-IT–Bioresources for sustainability), PORTUGAL 2020, grant number LISBOA-01-0145- FEDER-402-022125 and PDR2020 Operação 7.8.4, Recursos genéticos (PDR2020-784-042734).Peer reviewe