Visió històrica de la veterinària a partir d'un text del segle XVII

Inclou la reproducció fotocopiada de l'obra Llibre dels secrets de Agricultura, casa rústica y pastoril. Per Fra Miquel Agustí. Barcelona: Esteve Liberós, 1617.Treball presentat a l'assignatura de Deontologia i Veterinària Legal (21223

Assessing the role of livestock and sympatric wild ruminants in spreading antimicrobial resistant Campylobacter and Salmonella in alpine ecosystems

Background: Livestock play an important role as reservoir of enteric pathogens and antimicrobial resistance (AMR), a health and economic concern worldwide. However, little is known regarding the transmission and maintenance of these pathogens at the wildlife-livestock interface. In this study, we assessed the occurrence, genetic diversity and AMR of Campylobacter spp. and Salmonella spp. shed by sympatric free-ranging livestock and a wild herbivore in an alpine ecosystem. Results: Campylobacter spp. was isolated from 23.3 % of cattle and 7.7 % of sheep but was not isolated from horses nor Pyrenean chamois (Rupicapra pyrenaica). Campylobacter jejuni was the most frequent species. A high genetic diversity and certain host specificity of C. jejuni isolates was observed. The main AMR detected in Campylobacter isolates was to nalidixic acid (88.2 %), ciprofloxacin (82.4 %) and tetracycline (82.4 %); only 11.7 % of the isolates were pan-susceptible and 17.6 % were multi-resistant. Salmonella ser. Newport was isolated only from one Pyrenean chamois and was pan-susceptible. Conclusions: Results show that free-ranging cattle and sheep are spreaders of Campylobacter as well as their AMR strains in the alpine environment. Therefore, contaminated alpine pastures or streams may constitute a source for the dissemination of AMR enteropathogens. However, apparently, alpine wild ungulates such as Pyrenean chamois play a negligible role in the epidemiology of zoonotic enteropathogens and AMR, and are not potential bioindicators of the burden of alpine environments.info:eu-repo/semantics/publishedVersio

e14a2 Transcript Favors Treatment-Free Remission in Chronic Myeloid Leukemia When Associated with Longer Treatment with Tyrosine Kinase Inhibitors and Sustained Deep Molecular Response

BCR-ABL fusion gene; Chronic myeloid leukemia; Tyrosine kinase inhibitorsGen de fusión BCR-ABL; Leucemia mieloide crónica; Inhibidores de la tirosina quinasaGen de fusió BCR-ABL; Leucèmia mieloide crònica; Inhibidors de la tirosina cinasae13a2 and e14a2 are the most frequent transcript types of the BCR::ABL1 fusion gene in chronic myeloid leukemia (CML). The current goal with tyrosine kinase inhibitors (TKI) is to achieve sustained deep molecular response (DMR) in order to discontinue TKI treatment and remain in the so-called treatment-free remission (TFR) phase, but biological factors associated with these goals are not well established. This study aimed to determine the effect of transcript type on TFR in patients receiving frontline treatment with imatinib (IM) or second-generation TKI (2G-TKI). Patients treated at least 119 months with IM presented less post-discontinuation relapse than those that discontinued IM before 119 months (p = 0.005). In addition, cases with the e14a2 transcript type treated at least 119 months with IM presented a better TFR (p = 0.024). On the other hand, the type of transcript did not affect the cytogenetic or molecular response in 2G-TKI treated patients; however, the use of 2G-TKI may be associated with higher and earlier DMR in patients with the e14a2 transcript.This work was supported in part by 2017 SGR288 (GRC) Generalitat de Catalunya. We thank the CERCA Programme/Generalitat de Catalunya, Josep Carreras International Foundation and “La Caixa” Foundation for institutional support

Application of multi-regression machine learning algorithms to solve ocean water mass mixing in the Atlantic Ocean

The distribution of any non-conservative variable in the deep open ocean results from the circulation and mixing of water masses (WMs) of contrasting origin and from the initial preformed composition, modified during ongoing simultaneous biological and/or geochemical processes. Estimating the contribution of the WMs composing a sample is useful to trace the distribution of each water mass and to quantitatively separate the physical (mixing) and biogeochemical components of the variability of any, non- conservative variable (e.g., dissolved organic carbon, prokaryote biomass) in the ocean. Other than potential temperature and salinity, additional semi-conservative and non-conservative variables have been used to solve the mixing of more than three water masses using Optimum Multi-Parameter (OMP) approaches. Successful application of an OMP analysis requires knowledge of the characteristics of the water masses in their source regions as well as their circulation and mixing patterns. Here, we propose the application of multi-regression machine learning models to solve ocean water mass mixing. The models tested were trained using the solutions from OMP analyses previously applied to samples from cruises in the Atlantic Ocean. Extremely Randomized Trees algorithm yielded the highest score (R2 = 0.9931; mse = 0.000227). Our model allows solving the mixing of water masses in the Atlantic Ocean using potential temperature, salinity, latitude, longitude and depth. Therefore, basic hydrographic data collected during typical research cruises or autonomous systems can be used as input variables and provide results in real time. The model can be fed with new solutions from compatible OMP analyses as well as with new water masses not previously considered in it. Our tool will provide knowledge on water mass composition and distribution to a broader community of marine scientists not specialized in OMP analysis and/or in the oceanography of the studied area. This will allow a quantitative analysis of the effect of water mass mixing on the variables or processes under study

Relative age effect on amateur football in Cantabria (Spain)

Título en portugués: Efeito da idade relativa no futebol amador da Cantabria (Espanha)[ES] El concepto efecto de la edad relativa (RAE) alude a una serie de ventajas que obtienen los jóvenes nacidos en los primeros meses del año deportivo respecto a los nacidos a finales de año, debido a su mayor grado de madurez física, mental y emocional. El objetivo del presente estudio consistió en verificar si el efecto de la edad relativa está presente en las categorías amateurs del fútbol español, o si por el contrario, este se va atenuando a medida que nos alejamos del análisis de los equipos de alto nivel. La muestra comprendía un total de 1488 jugadores pertenecientes a las 4 categorías de fútbol amateur en Cantabria (3ªDivisión, n=379; Regional Preferente, n=357; Primera Regional, n=340; y Segunda Regional, n=412). Los resultados indican que existe poco efecto del RAE en el conjunto total de todas las posiciones en función del trimestre de nacimiento, pero sí que existe un efecto grande en función del semestre. Así mismo, en función de la categoría existe un efecto claro en 3ªDivisión en función del trimestre de nacimiento. Por tanto y como conclusión general, podemos afirmar que el RAE tiene un menor impacto en el fútbol amateur que en el fútbol profesional.[EN] The term Relative Age Effect (RAE) refers to the advantages youths born in the first half of the year have as compared to the ones born in the second half, as a result of their greater levels of physical, psychological and emotional maturity. The main purpose of this study was to verify if Relative Age Effect has an impact on amateur football or if its influence weakens as the level of the competition decreases. The sample comprised all 1488 players of the four existing amateur soccer leagues in Cantabria (3ªDivisión, n=379; Regional Preferente, n=357; Primera Regional, n=340; y Segunda Regional, n=412). The main outcomes revealed that RAE has little effect on playing positions when analyzed by quartiles, but a bigger effect is detected when analyzed by semesters. Furthermore, RAE also has an effect on competitive levels. RAE shows a clear effect on 3ªDivisión when analyzed by quartiles. In conclusion, Relative Age Effect has a minor impact on amateur soccer as compared to elite soccer. Keywords: RAE; soccer; amateur.[PT] O conceito de efeito relativo da idade (RAE) refere-se a uma série de vantagens obtidas por jovens nascidos nos primeiros meses do ano esportivo, comparados aos nascidos no final do ano, devido ao maior grau de maturidade física, mental e emocional. O objetivo do presente estudo foi verificar se o efeito da idade relativa está presente nas categorias amadoras do futebol espanhol, ou se, ao contrário, isso é atenuado à medida que nos afastamos da análise de equipes de alto nível. A amostra incluiu um total de 1488 jogadores pertencentes às 4 categorias de futebol amador na Cantábria (3ª Divisão, n = 379; Regional Preferida, n = 357; Primeira Regional, n = 340; e Segunda Regional, n = 412). Os principais resultados indicam que há pouco efeito do RAE no conjunto total de todas as posições, dependendo do prazo de nascimento, mas há um grande efeito dependendo do semestre. Da mesma forma, dependendo da categoria, o RAE também tem um efeito claro na Terceira Divisão, dependendo do prazo de nascimento. Portanto, e como conclusão geral, podemos afirmar que o Efeito da Idade Relativa tem menor impacto no futebol amador do que no futebol profissional.S

Classical Swine Fever Virus vs. Classical Swine Fever Virus : the Superinfection Exclusion Phenomenon in Experimentally Infected Wild Boar

Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms

African swine fever virus infection in Classical swine fever subclinically infected wild boars

Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression.This research was supported by grants AGL2013–48998 and AGL2015–66907 from the Spanish government. S.M and A.C ha

A Phase I-II multicenter trial with Avelumab plus autologous dendritic cell vaccine in pre-treated mismatch repair-proficient (MSS) metastatic colorectal cancer patients; GEMCAD 1602 study

[Background]: Immune check-point blockade (ICB) has shown clinical beneft in mismatch repair-defcient/microsatellite instability high metastatic colorectal cancer (mCRC) but not in mismatch repair-profcient/microsatellite stable patients. Cancer vaccines with autologous dendritic cells (ADC) could be a complementary therapeutic approach to ICB as this combination has the potential to achieve synergistic efects. [Methods]: This was a Phase I/II multicentric study with translational sub-studies, to evaluate the safety, pharmacodynamics and anti-tumor efects of Avelumab plus ADC vaccine in heavily pre-treated MSS mCRC patients. Primary objective was to determine the maximum tolerated dose and the efcacy of the combination. The primary end-point was 40% progressionfree survival at 6 months with a 2 Simon Stage. [Results]: A total of 28 patients were screened and 19 pts were included. Combined therapy was safe and well tolerated. An interim analysis (Simon design frst-stage) recommended early termination because only 2/19 (11%) patients were disease free at 6 months. Median PFS was 3.1 months [2.1–5.3 months] and overall survival was 12.2 months [3.2–23.2 months]. Stimulation of immune system was observed in vitro but not clinically. The evaluation of basal RNA-seq noted signifcant changes between pre and post-therapy liver biopsies related to lipid metabolism and transport, infammation and oxidative stress pathways. [Conclusions]: The combination of Avelumab plus ADC vaccine is safe and well tolerated but exhibited modest clinical activity. Our study describes, for the frst-time, a de novo post-therapy metabolic rewiring, that could represent novel immunotherapyinduced tumor vulnerabilities.The study was funded by grants from the FIS PI17/00732 from Instituto de Salud Carlos III, Premi Fi de Residència Emili Letang from Hospital Clínic Barcelona, Plan Nacional de I + D (PID-107139RB-C21 to DB-R and PID2020-115051RB-I00 to MC) and Grupo Español Multidisciplinar en Cáncer Digestivo (GEMCAD). The study was funded with Grants from Catalan Agency for Management of University and Research Grants (AGAUR) (2014-SGR-474, 2017-SGR-1174 and 2017-SGR-1033), Fundació la Marató de TV3 (201330.10), Instituto de Salud Carlos III (PI13/01728 and PI19/00740) and Fundacion Olga Torres (Modalitat A. 2019/2020) to JM. IMMETCOLS signature is under patent protection (EP21382772.8.) This research was financially supported by GEMCAD and (OR Avelumab was provided) by Merck, S.L.U., Madrid, Spain, an affiliate of Merck KGaA, Darmstadt, Germany, as part of an alliance between the healthcare business of Merck KGaA, Darmstadt, Germany (CrossRef Funder ID: https://doi.org/10.13039/100009945) and Pfizer