The Function Acquisition Speed Test (FAST) as a Measure of Verbal Stimulus Relations in the Context of Condom Use

The Function Acquisition Speed Test (FAST) has shown recent evidence as an effective tool for the quantification of stimulus relatedness. The current study assessed the potential of the FAST in measuring the effects of the presentation of positively or negatively valenced messages on relatedness between stimulus relations with regard to safe-sex behavior, namely condom use. Fifty-one participants were assigned to one of three conditions comprised of valenced message interventions regarding the impact of condom use on the enjoyment of sexual behavior (each condition n = 17): a positive-message condition, a negative-message condition, or a no-message control condition. A significant Strength of Relation (SoR) score was found across positive and negative FAST test trials in the positive-message condition only, with no significant differences in SoR scores observed for either the Negative-message or Control conditions. These data suggest that the FAST may have utility as a sensitive behavioral tool for measuring changes in stimulus relations concerning safe-sex behavior on the basis of brief message interventions

Nuclear Quadrupole Hyperfine Structure in HC14N/H14NC and DC15N/D15NC Isomerization: A Diagnostic Tool for Characterizing Vibrational Localization

Large-amplitude molecular motions which occur during isomerization can cause significant changes in electronic structure. These variations in electronic properties can be used to identify vibrationally-excited eigenstates which are localized along the potential energy surface. This work demonstrates that nuclear quadrupole hyperfine interactions can be used as a diagnostic marker of progress along the isomerization path in both the HC14N/H14NC and DC15N/D15NC chemical systems. Ab initio calculations at the CCSD(T)/cc-pCVQZ level indicate that the hyperfine interaction is extremely sensitive to the chemical bonding of the quadrupolar 14N nucleus and can therefore be used to determine in which potential well the vibrational wavefunction is localized. A natural bonding orbital analysis along the isomerization path further demonstrates that hyperfine interactions arise from the asphericity of the electron density at the quadrupolar nucleus. Using the CCSD(T) potential surface, the quadrupole coupling constants of highly-excited vibrational states are computed from a one-dimensional internal coordinate path Hamiltonian. The excellent agreement between ab initio calculations and recent measurements demonstrates that nuclear quadrupole hyperfine structure can be used as a diagnostic tool for characterizing localized HCN and HNC vibrational states.Comment: Accepted by Physical Chemistry Chemical Physic

rpSPH: a novel Smoothed Particle Hydrodynamics Algorithm

We suggest a novel discretisation of the momentum equation for Smoothed Particle Hydrodynamics (SPH) and show that it significantly improves the accuracy of the obtained solutions. Our new formulation which we refer to as relative pressure SPH, rpSPH, evaluates the pressure force in respect to the local pressure. It respects Newtons first law of motion and applies forces to particles only when there is a net force acting upon them. This is in contrast to standard SPH which explicitly uses Newtons third law of motion continuously applying equal but opposite forces between particles. rpSPH does not show the unphysical particle noise, the clumping or banding instability, unphysical surface tension, and unphysical scattering of different mass particles found for standard SPH. At the same time it uses fewer computational operations. and only changes a single line in existing SPH codes. We demonstrate its performance on isobaric uniform density distributions, uniform density shearing flows, the Kelvin-Helmholtz and Rayleigh-Taylor instabilities, the Sod shock tube, the Sedov-Taylor blast wave and a cosmological integration of the Santa Barbara galaxy cluster formation test. rpSPH is an improvement these cases. The improvements come at the cost of giving up exact momentum conservation of the scheme. Consequently one can also obtain unphysical solutions particularly at low resolutions.Comment: 17 pages, 13 figures. Final version. Including section of how to break i

Ranking hazards pertaining to human health concerns from land application of anaerobic digestate

peer-reviewedAnaerobic digestion (AD) has been identified as one of the cleanest producers of green energy. AD typically uses organic materials as feedstock and, through a series of biological processes, produces methane. Farmyard manure and slurry (FYM&S) are important AD feedstock and are typically mixed with agricultural waste, grass and/or food wastes. The feedstock may contain many different pathogens which can survive the AD process and hence also possibly be present in the final digestate. In this study, a semi-quantitative screening tool was developed to rank pathogens of potential health concern emerging from AD digestate. A scoring system was used to categorise likely inactivation during AD, hazard pathways and finally, severity as determined from reported human mortality rates, number of global human-deaths and infections per 100,000 populations. Five different conditions including mesophilic and thermophilic AD and three different pasteurisation conditions were assessed in terms of specific pathogen inactivation. In addition, a number of scenarios were assessed to consider foodborne incidence data from Ireland and Europe and to investigate the impact of raw FYM&S application (without AD and pasteurisation). A sensitivity analysis revealed that the score for the mortality rate (S3) was the most sensitive parameter (rank coefficient 0.49) to influence the final score S; followed by thermal inactivation score (S1, 0.25) and potential contamination pathways (S2, 0.16). Across all the scenarios considered, the screening tool prioritised Cryptosporidium parvum, Salmonella spp., norovirus, Streptococcus pyogenes, enteropathogenic E. coli (EPEC), Mycobacterium spp., Salmonella typhi (followed by S. paratyphi), Clostridium spp., Listeria monocytogenes and Campylobacter coli as the highest-ranking pathogens of human health concern resulting from AD digestate in Ireland. This tool prioritises potentially harmful pathogens which can emerge from AD digestate and highlights where regulation and intervention may be required

A Bayesian inference approach to quantify average pathogen loads in farmyard manure and slurry using open-source Irish datasets

peer-reviewedFarm-to-fork quantitative microbial risk assessments (QMRA) typically start with a preliminary estimate of initial concentration (Cinitial) of microorganism loading at farm level, consisting of an initial estimate of prevalence (P) and the resulting pathogen levels in animal faeces. An average estimation of the initial concentration of pathogens can be achieved by combining P estimates in animal populations and the levels of pathogens in colonised animals' faeces and resulting cumulative levels in herd farmyard manure and slurry (FYM&S). In the present study, 14 years of data were collated and assessed using a Bayesian inference loop to assess the likely P of pathogens. In this regard, historical and current survey data exists on P estimates for a number of pathogens, including Cryptosporidium parvum, Mycobacterium avium subspecies paratuberculosis (MAP), Salmonella spp., Clostridium spp., Campylobacter spp., pathogenic E. coli, and Listeria monocytogenes in several species (cattle, pigs, and sheep) in Ireland. The results revealed that Cryptosporidium spp. has potentially the highest mean P (Pmean) (25.93%), followed by MAP (15.68%) and Campylobacter spp. (8.80%) for cattle. The Pmean of E. coli is highest (7.42%) in pigs, while the Pmean of Clostridium spp. in sheep was estimated to be 7.94%. Cinitial for Cryptosporidium spp., MAP., Salmonella spp., Clostridium spp., and Campylobacter spp. in cattle faeces were derived with an average of 2.69, 4.38, 4.24, 3.46, and 3.84 log10 MPN g −1, respectively. Average Cinitial of Cryptosporidium spp., Salmonella spp., Clostridium spp., and E. coli in pig slurry was estimated as 1.27, 3.12, 3.02, and 4.48 log10 MPN g −1, respectively. It was only possible to calculate the average Cinitial of Listeria monocytogenes in sheep manure as 1.86 log10 MPN g −1. This study creates a basis for future farm-to-fork risk assessment models to base initial pathogen loading values for animal faeces and enhance risk assessment efforts

Anaerobic digestion of agricultural manure and biomass – Critical indicators of risk and knowledge gaps

peer-reviewedAnaerobic digestion (AD) has been identified as a potential green technology to treat food and municipal waste, agricultural residues, including farmyard manure and slurry (FYM&S), to produce biogas. FYM&S and digestate can act as soil conditioners and provide valuable nutrients to plants; however, it may also contain harmful pathogens. This study looks at the critical indicators in determining the microbial inactivation potential of AD and the possible implications for human and environmental health of spreading the resulting digestate on agricultural land. In addition, available strategies for risk assessment in the context of EU and Irish legislation are assessed. Storage time and process parameters (including temperature, pH, organic loading rate, hydraulic retention time), feedstock recipe (carbon-nitrogen ratio) to the AD plant (both mesophilic and thermophilic) were all assessed to significantly influence pathogen inactivation. However, complete inactivation of all pathogens is unlikely. There are limited studies evaluating risks from FYM&S as a feedstock in AD and the spreading of resulting digestate. The lack of process standardisation and varying feedstocks between AD farms means risk must be evaluated on a case by case basis and calls for a more unified risk assessment methodology. In addition, there is a need for the enhancement of AD farm-based modelling techniques and datasets to help in advancing knowledge in this area.Department of Agriculture, Food and the Marin

Quantitative microbial risk assessment associated with ready-to-eat salads following the application of farmyard manure and slurry or anaerobic digestate to arable lands

peer-reviewedFarmyard manure and slurry (FYM&S) and anaerobic digestate are potentially valuable soil conditioners providing important nutrients for plant development and growth. However, these organic fertilisers may pose a microbial health risk to humans. A quantitative microbial risk assessment (QMRA) model was developed to investigate the potential human exposure to pathogens following the application of FYM&S and digestate to agricultural land. The farm-to-fork probabilistic model investigated the fate of microbial indicators (total coliforms and enterococci) and foodborne pathogens in the soil with potential contamination of ready-to-eat salads (RTEs) at the point of human consumption. The processes examined included pathogen inactivation during mesophilic anaerobic digestion (M-AD), post-AD pasteurisation, storage, dilution while spreading, decay in soil, post-harvest washing processes, and finally, the potential growth of the pathogen during refrigeration/storage at the retail level in the Irish context. The QMRA highlighted a very low annual probability of risk (Pannual) due to Clostridium perfringens, norovirus, and Salmonella Newport across all scenarios. Mycobacterium avium may result in a very high mean Pannual for the application of raw FYM&S, while Cryptosporidium parvum and pathogenic E. coli showed high Pannual, and Listeria monocytogenes displayed moderate Pannual for raw FYM&S application. The use of AD reduces this risk; however, pasteurisation reduces the Pannual to an even greater extent posing a very low risk. An overall sensitivity analysis revealed that mesophilic-AD's inactivation effect is the most sensitive parameter of the QMRA, followed by storage and the decay on the field (all negatively correlated to risk estimate). The information generated from this model can help to inform guidelines for policymakers on the maximum permissible indicator or pathogen contamination levels in the digestate. The QMRA can also provide the AD industry with a safety assessment of pathogenic organisms resulting from the digestion of FYM&S

Water masses and currents in the upper tropical Northeast Atlantic off Northwest Africa

Recent current measurements in the tropical eastern North Atlantic reproduce the components of the large scale flow field. However, the observations as well as the 1/12°-FLAME model computations indicate that a lot of eddy scale variability is superimposed on the mean flow field. Despite of the disturbance by variability the signature of the Guinea Dome is well present. In November 2002 the Guinea Dome transport from direct observations was about 2.8 Sv above σ θ = 25.8 kg/m3 and 4 Sv between σ θ = 25.8 and 27.1 kg/m3. The oxygen minimum in the shadow zone comprises the central water and the Antarctic Intermediate Water (AAIW) layers and is located between the equatorial current system and the North Equatorial Current. The North Equatorial Counter- and Undercurrents at 3° to 6°N are major oxygen sources for the central water layer of the low-oxygen regions in the northeastern tropical Atlantic. A second, northern North Equatorial Countercurrent (nNECC) band exists at 8° to 10°N. The nNECC carries oxygen rich water from the southern hemisphere eastward but with an admixture of water from the northern hemisphere. A float at 200 m depth was spreading eastward in the North Equatorial Undercurrent (NEUC), at 28°W it shifted northward into the nNECC, and then was trapped in the Guinea Dome region for more than 3 years. The model indicates the region 22° to 32°W as the area of exchange between the NECC/NEUC and the nNECC bands. In the AAIW layer the northern Intermediate Countercurrent acts as oxygen source for the oxygen minimum zone