MPA in Labor: Securing the Pearl Cays of Nicaragua

Implementation of Marine Protected Areas (MPAs) has always a step-zero, i.e., an initial phase when the idea is incepted, communicated and negotiated among stakeholders. What happens during this phase is likely to have an impact later on. If not done right, the management of the MPA may encounter problems at later stage that will be difficult to correct. Inspired by this working theory, this article describes the effort to establish the Pearl Cays off the Caribbean coast of Nicaragua as a protected area. This case-study illustrates the critical actions to be taken during step-zero, i.e., what needs to be considered and done before an MPA is formally declared. The area investigated consists of a number of small islands (cays) and coral reefs, fishing grounds and marine turtle nesting areas. Throughout history, the cays have played an important role in sustaining livelihoods of nearby communities. Although the idea of an MPA was originally conservation, the communities saw it as an opportunity to regain ownership and control of the cays. By Nicaraguan law, in order to establish protected areas, consultation and approval from local people is required. In the case of the Pearl Cays, this has proved difficult. The article demonstrates how MPA initiatives must sometimes relate to already ongoing complex social processes in the area where they are to be instigated

A finer grained approach to psychological capital and work performance

Purpose Psychological capital is a set of personal resources comprised by hope, efficacy, optimism, and resilience, which previous research has supported as being valuable for general work performance. However, in today’s organizations, a multidimensional approach is required to understanding work performance, thus, we aimed to determine whether psychological capital improves proficiency, adaptivity, and proactivity, and also whether hope, efficiency, resilience, and optimism have a differential contribution to the same outcomes. Analyzing the temporal meaning of each psychological capital dimension, this paper theorizes the relative weights of psychological capital dimensions on proficiency, adaptivity, and proactivity, proposing also that higher relative weight dimensions are helpful to cope with job demands and perform well. Methodology Two survey studies, the first based on cross-sectional data and the second on two waves of data, were conducted with employees from diverse organizations, who provided measures of their psychological capital, work performance, and job demands. Data was modeled with regression analysis together with relative weights analysis. Findings Relative weights for dimensions of psychological capital were supported as having remarkable unique contributions for proficient, adaptive, and proactive behavior, particularly when job demands were high. Originality/Value We concluded that organizations facing high job demands should implement actions to enhance psychological capital dimensions; however, those actions should focus on the specific criterion of performance of interest

Metal-Free ALS Variants of Dimeric Human Cu,Zn-Superoxide Dismutase Have Enhanced Populations of Monomeric Species

Amino acid replacements at dozens of positions in the dimeric protein human, Cu,Zn superoxide dismutase (SOD1) can cause amyotrophic lateral sclerosis (ALS). Although it has long been hypothesized that these mutations might enhance the populations of marginally-stable aggregation-prone species responsible for cellular toxicity, there has been little quantitative evidence to support this notion. Perturbations of the folding free energy landscapes of metal-free versions of five ALS-inducing variants, A4V, L38V, G93A, L106V and S134N SOD1, were determined with a global analysis of kinetic and thermodynamic folding data for dimeric and stable monomeric versions of these variants. Utilizing this global analysis approach, the perturbations on the global stability in response to mutation can be partitioned between the monomer folding and association steps, and the effects of mutation on the populations of the folded and unfolded monomeric states can be determined. The 2- to 10-fold increase in the population of the folded monomeric state for A4V, L38V and L106V and the 80- to 480-fold increase in the population of the unfolded monomeric states for all but S134N would dramatically increase their propensity for aggregation through high-order nucleation reactions. The wild-type-like populations of these states for the metal-binding region S134N variant suggest that even wild-type SOD1 may also be prone to aggregation in the absence of metals

Transcriptional Regulation of Human Dual Specificity Protein Phosphatase 1 (DUSP1) Gene by Glucocorticoids

Background: Glucocorticoids are potent anti-inflammatory agents commonly used to treat inflammatory diseases. They convey signals through the intracellular glucocorticoid receptor (GR), which upon binding to ligands, associates with genomic glucocorticoid response elements (GREs) to regulate transcription of associated genes. One mechanism by which glucocorticoids inhibit inflammation is through induction of the dual specificity phosphatase-1 (DUSP1, a.k.a. mitogen-activated protein kinase phosphatase-1, MKP-1) gene. Methodology/Principal Findings: We found that glucocorticoids rapidly increased transcription of DUSP1 within 10 minutes in A549 human lung adenocarcinoma cells. Using chromatin immunoprecipitation (ChIP) scanning, we located a GR binding region between 21421 and 21118 upstream of the DUSP1 transcription start site. This region is active in a reporter system, and mutagenesis analyses identified a functional GRE located between 21337 and 21323. We found that glucocorticoids increased DNase I hypersensitivity, reduced nucleosome density, and increased histone H3 and H4 acetylation within genomic regions surrounding the GRE. ChIP experiments showed that p300 was recruited to the DUSP1 GRE, and RNA interference experiments demonstrated that reduction of p300 decreased glucocorticoid-stimulated DUSP1 gene expression and histone H3 hyperacetylation. Furthermore, overexpression of p300 potentiated glucocorticoid-stimulated activity of a reporter gene containing the DUSP1 GRE, and this coactivation effect was compromised when the histone acetyltransferase domain was mutated. ChIP-reChIP experiments using GR followed by p300 antibodies showed significant enrichment of the DUSP1 GRE upon glucocorticoid treatment, suggesting that GR and p300 are in the same protein complex recruited to the DUSP1 GRE. Conclusions/Significance: Our studies identified a functional GRE for the DUSP1 gene. Moreover, the transcriptional activation of DUSP1 by glucocorticoids requires p300 and a rapid modification of the chromatin structure surrounding the GRE. Overall, understanding the mechanism of glucocorticoid-induced DUSP1 gene transcription could provide insights into therapeutic approaches against inflammatory diseases. © 2010 Shipp et al

Interoception in anxiety and depression

We review the literature on interoception as it relates to depression and anxiety, with a focus on belief, and alliesthesia. The connection between increased but noisy afferent interoceptive input, self-referential and belief-based states, and top-down modulation of poorly predictive signals is integrated into a neuroanatomical and processing model for depression and anxiety. The advantage of this conceptualization is the ability to specifically examine the interface between basic interoception, self-referential belief-based states, and enhanced top-down modulation to attenuate poor predictability. We conclude that depression and anxiety are not simply interoceptive disorders but are altered interoceptive states as a consequence of noisily amplified self-referential interoceptive predictive belief states

Role and regulation of MKP-1 in airway inflammation

Mitogen-activated protein kinase (MAPK) phosphatase 1 (MKP-1) is a protein with anti-inflammatory properties and the archetypal member of the dual-specificity phosphatases (DUSPs) family that have emerged over the past decade as playing an instrumental role in the regulation of airway inflammation. Not only does MKP-1 serve a critical role as a negative feedback effector, controlling the extent and duration of pro-inflammatory MAPK signalling in airway cells, upregulation of this endogenous phosphatase has also emerged as being one of the key cellular mechanism responsible for the beneficial actions of clinically-used respiratory medicines, including beta(2)-agonists, phosphodiesterase inhibitors and corticosteroids. Herein, we review the role and regulation of MKP-1 in the context of airway inflammation. We initially outline the structure and biochemistry of MKP-1 and summarise the multi-layered molecular mechanisms responsible for MKP-1 production more generally. We then focus in on some of the key in vitro studies in cell types relevant to airway disease that explain how MKP-1 can be regulated in airway inflammation at the transcriptional, post-translation and post-translational level. And finally, we address some of the potential challenges with MKP-1 upregulation that need to be explored further to fully exploit the potential of MKP-1 to repress airway inflammation in chronic respiratory disease

Relationship between fluid-escape pipes and hydrate distribution in offshore Sabah (NW Borneo)

Fluid-escape pipes represent seismic evidence for the focused cross-stratal migration of fluids. In natural gas hydrate systems, these features serve both as conduits for methane-rich fluids and as preferred locations for the formation of gas hydrates. In this study, 3D seismic, well-log and core data from offshore Sabah (NW Borneo) are used to investigate the controls on the occurrence of fluid-escape pipes and their impact on hydrate distribution in a system dominated by the vertical leakage of thermogenic hydrocarbons. The pipes are observed within a gas hydrate stability zone (GHSZ) that extends 100 m below a bottom simulating reflector (BSR), located at 155 m below the seafloor (mbsf). Pipes are restricted to an area with evidence of free gas-bearing sediments, suggesting a causative link where the free gas promotes the build-up of critical fluid pressures. The stacking of the upper terminus of fluid-escape pipes at discrete stratigraphic intervals suggests that fluid flow to the seabed has been episodically enhanced. Possible triggers for cyclical increases of pore fluid pressures are sea-level and temperature fluctuations, tectonic activity and gas leakage from deep reservoirs. This fluid flow system further impacts the gas hydrate distribution. The fluid-escape pipes can be locations where hydrates occur at high concentrations up to the seafloor if the pipe is presently active. Therefore, the observed up-bending of the stratigraphic reflections along the pipes are interpreted as a combination of a net volume increase of the host sediment owing to hydrate formation and seismic velocity pull-up effects. Away from the pipes, hydrates do not occur until 65–152 mbsf and are present only at low to moderate concentrations. At this site of focused fluid flow, fluid-escape pipes constitute, by volume, only 7–11% of the gas hydrate occurrence zone. Nevertheless, we predict that they could host between 20 and 50% of the whole hydrate volume. It is therefore likely that, in similar systems, a volumetrically significant portion of the total hydrate reservoir is hosted within fluid-escape pipes. The distribution of these features should thus be considered as a critical parameter for hydrate volume estimates.</p

Relationship between fluid-escape pipes and hydrate distribution in offshore Sabah (NW Borneo)

Fluid-escape pipes represent seismic evidence for the focused cross-stratal migration of fluids. In natural gas hydrate systems, these features serve both as conduits for methane-rich fluids and as preferred locations for the formation of gas hydrates. In this study, 3D seismic, well-log and core data from offshore Sabah (NW Borneo) are used to investigate the controls on the occurrence of fluid-escape pipes and their impact on hydrate distribution in a system dominated by the vertical leakage of thermogenic hydrocarbons. The pipes are observed within a gas hydrate stability zone (GHSZ) that extends 100 m below a bottom simulating reflector (BSR), located at 155 m below the seafloor (mbsf). Pipes are restricted to an area with evidence of free gas-bearing sediments, suggesting a causative link where the free gas promotes the build-up of critical fluid pressures. The stacking of the upper terminus of fluid-escape pipes at discrete stratigraphic intervals suggests that fluid flow to the seabed has been episodically enhanced. Possible triggers for cyclical increases of pore fluid pressures are sea-level and temperature fluctuations, tectonic activity and gas leakage from deep reservoirs. This fluid flow system further impacts the gas hydrate distribution. The fluid-escape pipes can be locations where hydrates occur at high concentrations up to the seafloor if the pipe is presently active. Therefore, the observed up-bending of the stratigraphic reflections along the pipes are interpreted as a combination of a net volume increase of the host sediment owing to hydrate formation and seismic velocity pull-up effects. Away from the pipes, hydrates do not occur until 65–152 mbsf and are present only at low to moderate concentrations. At this site of focused fluid flow, fluid-escape pipes constitute, by volume, only 7–11% of the gas hydrate occurrence zone. Nevertheless, we predict that they could host between 20 and 50% of the whole hydrate volume. It is therefore likely that, in similar systems, a volumetrically significant portion of the total hydrate reservoir is hosted within fluid-escape pipes. The distribution of these features should thus be considered as a critical parameter for hydrate volume estimates.</p