330 research outputs found
Reconfigurable microfluidic circuits for isolating and retrieving cells of interest
Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this problem that exploits the remarkable properties of microfluidic circuits with fluid walls─transparent interfaces between culture media and an immiscible fluorocarbon that are easily pierced with pipets. We provide two proofs of concept in which specific cell subpopulations are isolated and recovered: (i) murine macrophages chemotaxing toward complement component 5a and (ii) bacteria (Pseudomonas aeruginosa) in developing biofilms that migrate toward antibiotics. We build circuits in minutes on standard Petri dishes, add cells, pump in laminar streams so molecular diffusion creates attractant gradients, acquire time-lapse images, and isolate desired subpopulations in real time by building fluid walls around migrating cells with an accuracy of tens of micrometers using 3D printed adaptors that convert conventional microscopes into wall-building machines. Our method allows live cells of interest to be easily extracted from microfluidic devices for downstream analyses
Consistent histories, the quantum Zeno effect, and time of arrival
We present a decomposition of the general quantum mechanical evolution
operator, that corresponds to the path decomposition expansion, and interpret
its constituents in terms of the quantum Zeno effect (QZE). This decomposition
is applied to a finite dimensional example and to the case of a free particle
in the real line, where the possibility of boundary conditions more general
than those hitherto considered in the literature is shown. We reinterpret the
assignment of consistent probabilities to different regions of spacetime in
terms of the QZE. The comparison of the approach of consistent histories to the
problem of time of arrival with the solution provided by the probability
distribution of Kijowski shows the strength of the latter point of view
Quantum Anti-Zeno Effect
We demonstrate that near threshold decay processes may be accelerated by
repeated measurements. Examples include near threshold photodetachment of an
electron from a negative ion, and spontaneous emission in a cavity close to the
cutoff frequency, or in a photon band gap material.Comment: 4 pages, 3 figure
Weisskopf-Wigner Decay Theory for the Energy-Driven Stochastic Schr\"odinger Equation
We generalize the Weisskopf-Wigner theory for the line shape and transition
rates of decaying states to the case of the energy-driven stochastic
Schr\"odinger equation that has been used as a phenomenology for state vector
reduction. Within the standard approximations used in the Weisskopf-Wigner
analysis, and assuming that the perturbing potential inducing the decay has
vanishing matrix elements within the degenerate manifold containing the
decaying state, the stochastic Schr\"odinger equation linearizes. Solving the
linearized equations, we find no change from the standard analysis in the line
shape or the transition rate per unit time. The only effect of the stochastic
terms is to alter the early time transient behavior of the decay, in a way that
eliminates the quantum Zeno effect. We apply our results to estimate
experimental bounds on the parameter governing the stochastic effects.Comment: 29 pages in RevTeX, Added Note, references adde
Projection Postulate and Atomic Quantum Zeno Effect
The projection postulate has been used to predict a slow-down of the time
evolution of the state of a system under rapidly repeated measurements, and
ultimately a freezing of the state. To test this so-called quantum Zeno effect
an experiment was performed by Itano et al. (Phys. Rev. A 41, 2295 (1990)) in
which an atomic-level measurement was realized by means of a short laser pulse.
The relevance of the results has given rise to controversies in the literature.
In particular the projection postulate and its applicability in this experiment
have been cast into doubt. In this paper we show analytically that for a wide
range of parameters such a short laser pulse acts as an effective level
measurement to which the usual projection postulate applies with high accuracy.
The corrections to the ideal reductions and their accumulation over n pulses
are calculated. Our conclusion is that the projection postulate is an excellent
pragmatic tool for a quick and simple understanding of the slow-down of time
evolution in experiments of this type. However, corrections have to be
included, and an actual freezing does not seem possible because of the finite
duration of measurements.Comment: 25 pages, LaTeX, no figures; to appear in Phys. Rev.
Distributed phase-covariant cloning with atomic ensembles via quantum Zeno dynamics
We propose an interesting scheme for distributed orbital state quantum
cloning with atomic ensembles based on the quantum Zeno dynamics. These atomic
ensembles which consist of identical three-level atoms are trapped in distant
cavities connected by a single-mode integrated optical star coupler. These
qubits can be manipulated through appropriate modulation of the coupling
constants between atomic ensemble and classical field, and the cavity decay can
be largely suppressed as the number of atoms in the ensemble qubits increases.
The fidelity of each cloned qubit can be obtained with analytic result. The
present scheme provides a new way to construct the quantum communication
network.Comment: 5 pages, 4 figure
Primordialists and Constructionists: a typology of theories of religion
This article adopts categories from nationalism theory to classify theories of religion. Primordialist explanations are grounded in evolutionary psychology and emphasize the innate human demand for religion. Primordialists predict that religion does not decline in the modern era but will endure in perpetuity. Constructionist theories argue that religious demand is a human construct. Modernity initially energizes religion, but subsequently undermines it. Unpacking these ideal types is necessary in order to describe actual theorists of religion. Three distinctions within primordialism and constructionism are relevant. Namely those distinguishing: a) materialist from symbolist forms of constructionism; b) theories of origins from those pertaining to the reproduction of religion; and c) within reproduction, between theories of religious persistence and secularization. This typology helps to make sense of theories of religion by classifying them on the basis of their causal mechanisms, chronology and effects. In so doing, it opens up new sightlines for theory and research
Measurement of the Charged Multiplicities in b, c and Light Quark Events from Z0 Decays
Average charged multiplicities have been measured separately in , and
light quark () events from decays measured in the SLD experiment.
Impact parameters of charged tracks were used to select enriched samples of
and light quark events, and reconstructed charmed mesons were used to select
quark events. We measured the charged multiplicities:
,
, from
which we derived the differences between the total average charged
multiplicities of or quark events and light quark events: and . We compared
these measurements with those at lower center-of-mass energies and with
perturbative QCD predictions. These combined results are in agreement with the
QCD expectations and disfavor the hypothesis of flavor-independent
fragmentation.Comment: 19 pages LaTex, 4 EPS figures, to appear in Physics Letters
An Isovelocity Dynamometer Method to Determine Monoarticular and Biarticular Muscle Parameters
Characterization of the clinical and immunologic phenotype and management of 157 individuals with 56 distinct heterozygous NFKB1 mutations
Background: An increasing number of NFKB1 variants are being identified in patients with heterogeneous immunologic phenotypes. Objective: To characterize the clinical and cellular phenotype as well as the management of patients with heterozygous NFKB1 mutations. Methods: In a worldwide collaborative effort, we evaluated 231 individuals harboring 105 distinct heterozygous NFKB1 variants. To provide evidence for pathogenicity, each variant was assessed in silico; in addition, 32 variants were assessed by functional in vitro testing of nuclear factor of kappa light polypeptide gene enhancer in B cells (NF-kappa B) signaling. Results: We classified 56 of the 105 distinct NFKB1 variants in 157 individuals from 68 unrelated families as pathogenic. Incomplete clinical penetrance (70%) and age-dependent severity of NFKB1-related phenotypes were observed. The phenotype included hypogammaglobulinemia (88.9%), reduced switched memory B cells (60.3%), and respiratory (83%) and gastrointestinal (28.6%) infections, thus characterizing the disorder as primary immunodeficiency. However, the high frequency of autoimmunity (57.4%), lymphoproliferation (52.4%), noninfectious enteropathy (23.1%), opportunistic infections (15.7%), autoinflammation (29.6%), and malignancy (16.8%) identified NF-kappa B1-related disease as an inborn error of immunity with immune dysregulation, rather than a mere primary immunodeficiency. Current treatment includes immunoglobulin replacement and immunosuppressive agents. Conclusions: We present a comprehensive clinical overview of the NF-kappa B1-related phenotype, which includes immunodeficiency, autoimmunity, autoinflammation, and cancer. Because of its multisystem involvement, clinicians from each and every medical discipline need to be made aware of this autosomal-dominant disease. Hematopoietic stem cell transplantation and NF-kappa B1 pathway-targeted therapeutic strategies should be considered in the future.Peer reviewe
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