Beyond Logic and Norms: A Figurational Critique of Institutional Theory in Organisation Studies

This paper provides a figurational critique of one of the most dominant theoretical frames within organisation studies - institutional theory. Despite its status as the leading theoretical lens for explaining organisational change, institutional theorists continue to struggle with the so called agency-structure issue and remain divided in how to overcome it. Our primary criticisms concern the propensity to invoke or generate dualisms, the reliance on the sociological frames which sustain this, and the failure to engage in any comprehensive way with Elias’s writings on this subject

The identification and heterologous expression of the biosynthetic gene cluster encoding the antibiotic and anticancer agent marinomycin

With the rise in antimicrobial resistance, there is an urgent need for new classes of antibiotic with which to treat infectious disease. Marinomycin, a polyene antibiotic from a marine microbe, has been shown capable of killing methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VREF), as well as having promising activity against melanoma. An attractive solution to the photoprotection of this antibiotic has been demonstrated. Here, we report the identification and analysis of the marinomycin biosynthetic gene cluster (BGC), and the biosynthetic assembly of the macrolide. The marinomycin BGC presents a challenge in heterologous expression due to its large size and high GC content, rendering the cluster prone to rearrangement. We demonstrate the transformation of Streptomyces lividans using a construct containing the cluster, and the heterologous expression of the encoded biosynthetic machinery and production of marinomycin B