Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae

The reliable production of marine fish larvae is one of the major bottlenecks in aquaculture due to high mortalities mainly caused by infectious diseases. To evaluate if the compound poly-β-hydroxybutyrate (PHB) might be a suitable immunoprophylactic measure in fish larviculture, its capacity to improve immunity and performance in European sea bass (Dicentrarchus labrax) yolk-sac larvae was explored. PHB was applied from mouth opening onwards to stimulate the developing larval immune system at the earliest possible point in time. Larval survival, growth, microbiota composition, gene expression profiles and disease resistance were assessed. PHB administration improved larval survival and, furthermore, altered the larva-associated microbiota composition. The bacterial challenge test using pathogenic Vibrio anguillarum revealed that the larval disease resistance was not influenced by PHB. The expression profiles of 26 genes involved e.g. in the immune response showed that PHB affected the expression of the antimicrobial peptides ferritin (fer) and dicentracin (dic), however, the response to PHB was inconsistent and weaker than previously demonstrated for sea bass post-larvae. Hence, the present study highlights the need for more research focusing on the immunostimulation of different early developmental stages for gaining a more comprehensive picture and advancing a sustainable production of high quality fry

What do your eyes reveal about your foreign language? Reading emotional sentences in a native and foreign language

Foreign languages are often learned in emotionally neutral academic environments which differ greatly from the familiar context where native languages are acquired. This difference in learning contexts has been argued to lead to reduced emotional resonance when confronted with a foreign language. In the current study, we investigated whether the reactivity of the sympathetic nervous system in response to emotionally-charged stimuli is reduced in a foreign language. To this end, pupil sizes were recorded while reading aloud emotional sentences in the native or foreign language. Additionally, subjective ratings of emotional impact were provided after reading each sentence, allowing us to further investigate foreign language effects on explicit emotional understanding. Pupillary responses showed a larger effect of emotion in the native than in the foreign language. However, such a difference was not present for explicit ratings of emotionality. These results reveal that the sympathetic nervous system reacts differently depending on the language context, which in turns suggests a deeper emotional processing when reading in a native compared to a foreign language.This research was partially supported by grants from the Spanish Government (PSI2014-52181-P, PSI2015-65689-P, PSI2011-23033 and SEV-2015-0490) (http://www.mineco.gob.es/portal/site/mineco/), from the Catalan Government (SGR-2014SGR1210), from the 7th Framework Programme (AThEME 613465) (http://www.atheme.eu/), and a 2016 BBVA Foundation Grant for Researchers and Cultural Creators (http://www.fbbva.es/) awarded to JAD. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

A model-based approach to associate complexity and robustness in engineering systems

Ever increasing functionality and complexity of products and systems challenge development companies in achieving high and consistent quality. A model-based approach is used to investigate the relationship between system complexity and system robustness. The measure for complexity is based on the degree of functional coupling and the level of contradiction in the couplings. Whilst Suh’s independence axiom states that functional independence (uncoupled designs) produces more robust designs, this study proves this not to be the case for max-/min-is-best requirements, and only to be true in the general sense for nominal-is-best requirements. In specific cases, the independence axiom has exceptions as illustrated with a machining example, showing how a coupled solution is more robust than its uncoupled counterpart. This study also shows with statistical significance, that for max- and min-is-best requirements, the robustness is most affected by the level of contradiction between coupled functional requirements (p = 1.4e−36). In practice, the results imply that if the main influencing factors for each function in a system are known in the concept phase, an evaluation of the contradiction level can be used to evaluate concept robustness.Novo Nordisk Foundatio

Equilibrium moisture content of radiata pine at elevated temperature and pressure reveals measurement challenges

Relatively few studies have been performed on the equilibrium moisture content (EMC) of wood under conditions of elevated temperature and pressure. Eight studies indicated that EMC near saturation decreased between 100 and 150 A degrees C, whilst five studies indicated that EMC increased. The aim of this study was to identify the likely source of the disagreement using radiata pine (Pinus radiata D. Don) sapwood which was conditioned to a moisture content of around 3 % and then exposed for 1 h at 150 A degrees C and relative humidities of either 50, 70 or 90 %. Mean values of EMC, obtained through in situ gravimetric analyses, were 5.7, 7.6 and 12.6 % with 95 % confidence intervals of the order of 1 %. In two further experiments, the humidity was allowed to rise briefly above 90 % and the moisture content after 1 h was found to be > 30 % as in the five studies that indicated EMC increased above 100 A degrees C. The high moisture contents were attributed to condensation of liquid water on the specimen with subsequent evaporation at a rate that was too slow for the moisture content to reach equilibrium before it was measured. Reliable EMC data at elevated temperatures require (1) tight process control of experimental conditions with minimal standard error, (2) specimens with low initial moisture content to avoid unwanted wood mass loss over time, (3) a relative humidity upper limit that avoids drift above 95 %, and (4) extrapolation of data to humidity approaching 100 %

Potential Contribution of SIM2 and ETS2 Functional Polymorphisms in Down Syndrome Associated Malignancies

Proper expression and functioning of transcription factors (TFs) are essential for regulation of different traits and thus could be crucial for the development of complex diseases. Subjects with Down syndrome (DS) have a higher incidence of acute lymphoblastic leukemia (ALL) while solid tumors, like breast cancer (BC) and oral cancer (OC), show rare incidences. Triplication of the human chromosome 21 in DS is associated with altered genetic dosage of different TFs. V-ets erythroblastosis virus E26 oncogene homolog 2 (ETS2) and Single Minded 2 (SIM2) are two such TFs that regulate several downstream genes involved in developmental and neurological pathways. Here we studied functional genetic polymorphisms (fSNP) in ETS2 and SIM2 encoding genes in a group of patients and control subjects to better understand association of these variants with DS phenotypes.We employed an in silico approach to identify potential target pathways of ETS2 and SIM2. fSNPs in genes encoding for these two TFs were identified using available databases. Selected sites were genotyped in individuals with DS, their parents, ALL, BC, OC as well as ethnically matched control individuals. We further analyzed these data by population-based statistical methods.Allelic/genotypic association analysis showed significant (P < 0.03) differences of rs2070530, rs1051476, rs11254, rs711 for DS subjects compared to control. rs711 also exhibited significantly different genotypic distribution pattern in parents of DS probands (P < 0.02) and BC patients (P < 0.02). Interaction analysis revealed independent main effect of rs711 in all the groups, while rs11254 exhibited independent main effect in DS subjects only. High entropy values were noticed for rs461155 in the solid tumor groups. Significant interactive effects of rs2070531 with rs1051475, rs1051476, rs11254 were observed in all the groups except DS. We infer from the present investigation that the difference in frequencies of fSNPs and their independent as well as interactive effects may be the cause for altered expression of SIM2 and ETS2 in DS and malignant groups, which affects different downstream biological pathways. Thus, altered expression of SIM2 and ETS2 could be one of the reasons for variable occurrence of different malignant conditions in DS