When veiled silences speak: reflexivity, trouble and repair as methodological tools for interpreting the unspoken in discourse-based data

Researchers who have attempted to make sense of silence in data have generally considered literal silences or such things as laughter. We consider the analysis of veiled silences where participants speak, but their speaking serves as ‘noise’ that ‘veils’, or masks, their inability or unwillingness to talk about a (potentially sensitive) topic. Extending Lisa Mazzei’s ‘problematic of silence’ by using our performativity-performance analytical method, we propose the purposeful use of ‘unusual conversational moves’, the deployment of researcher reflexivity, and the analysis of trouble and repair as methods to expose taken-for-granted normative frameworks in veiled silences. We illustrate the potential of these research practices through reference to our study on men’s involvement in reproductive decision-making, in which participants demonstrated an inability to engage with the topic. The veiled silence that this produced, together with what was said, pointed to the operation of procreative heteronormativity

Anarchy in the UK: Detailed genetic analysis of worker reproduction in a naturally occurring British anarchistic honeybee, Apis mellifera, colony using DNA microsatellites

Anarchistic behaviour is a very rare phenotype of honeybee colonies. In an anarchistic colony, many workers’ sons are reared in the presence of the queen. Anarchy has previously been described in only two Australian colonies. Here we report on a first detailed genetic analysis of a British anarchistic colony. Male pupae were present in great abundance above the queen excluder, which was clearly indicative of extensive worker reproduction and is the hallmark of anarchy. Seventeen microsatellite loci were used to analyse these male pupae, allowing us to address whether all the males were indeed workers’ sons, and how many worker patrilines and individual workers produced them. In the sample, 95 of 96 of the males were definitely workers’ sons. Given that ≈ 1% of workers’ sons were genetically indistinguishable from queen’s sons, this suggests that workers do not move any queen-laid eggs between the part of the colony where the queen is present to the area above the queen excluder which the queen cannot enter. The colony had 16 patrilines, with an effective number of patrilines of 9.85. The 75 males that could be assigned with certainty to a patriline came from 7 patrilines, with an effective number of 4.21. They were the offspring of at least 19 workers. This is in contrast to the two previously studied Australian naturally occurring anarchist colonies, in which most of the workers’ sons were offspring of one patriline. The high number of patrilines producing males leads to a low mean relatedness between laying workers and males of the colony. We discuss the importance of studying such colonies in the understanding of worker policing and its evolution

Unusual presentation of cactus spines in the flank of an elderly man: a case report

<p>Abstract</p> <p>Introduction</p> <p>Splinters and spines of plant matter are common foreign bodies in skin wounds of the extremities, and often present embedded in the dermis or subcutaneous tissue. Vegetative foreign bodies are highly inflammatory and, if not completely removed, can cause infection, toxic reactions, or granuloma formation. Older patients are at increased risk for infection from untreated plant foreign bodies. The most common error in plant splinter and spine management is failure to detect their presence.</p> <p>Case presentation</p> <p>Here we report a case of cactus spines in an 84-year-old Caucasian man presenting on the right flank as multiple, red papules with spiny extensions. This presentation was unusual both in location and the spinous character of the lesions, and only after punch biopsy analysis was a diagnosis of cactus matter spines made.</p> <p>Conclusions</p> <p>Our patient presented with an unusual case of cactus spines that required histopathology for identification. Skin lesions with neglected foreign bodies are a common cause of malpractice claims. If not removed, foreign bodies of the skin, particularly in elderly individuals, can result in inflammatory and infectious sequela. This report underscores the importance of thoroughly evaluating penetrating skin lesions for the presence of foreign bodies, such as splinters and spines.</p

The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots

Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability. We present the expression characteristics of SlREO, a novel gene isolated from tomato (Solanum lycopersicum L.). This gene was highly expressed in roots but had a very low level of expression in aerial plant organs. A 2.4-kb region representing the SlREO promoter sequence was cloned upstream of the uidA GUS reporter gene and shown to direct expression in the root cortex. In mature, glasshouse-grown plants this strict root specificity was maintained. Furthermore, promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid. The predicted protein sequence of SlREO contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. The novel SlREO promoter has properties ideal for applications requiring strong and specific gene expression in the bulk of tomato root tissue growing in soil, and is also likely to be useful in other Solanaceous crop

Testing the efficacy of voluntary urban greenhouse gas emissions inventories

Drawing from an original dataset of urban metropolitan carbon footprints, we explore the correlations between national level climate change commitments and subnational level inventories. We ask: Does voluntary reporting allow a city to perform better than national average? Does ambitiousness in commitment have an impact on performance in footprint reduction? Does having long-term commitments affect performance in footprint reduction? Do binding national level commitments (such as those under the Kyoto Protocol) affect performance at the city level in terms of footprint reduction? To provide answers, we synthesize data from the largest repository of voluntary sub-national commitments and actions towards footprint reduction and greenhouse gas inventories from around the world, the Carbonn platform. More than 500 cities report at least one action, commitment or inventory to this database. We find, using a subset of this database, perhaps counter intuitively that cities with more ambitious commitments do not necessarily have steeper reductions in emissions. Our data also suggest that having long-term self-reported goals does not make the cities perform better in terms of footprint reduction. This appears to be true for both government and community commitments reported. Lastly, and positively, our data did reveal a statistically significant effect for cities belonging to countries that had committed to the Kyoto Protocol, suggesting the necessity of binding national (and supranational) climate targets

Impacts of Habitat Degradation on Tropical Montane Biodiversity and Ecosystem Services: A Systematic Map for Identifying Future Research Priorities

Tropical montane forests (TMFs) are major centers of evolutionary change and harbor many endemic species with small geographic ranges. In this systematic map, we focus on the impacts of anthropogenic habitat degradation on TMFs globally. We first determine how TMF research is distributed across geographic regions, degradation type (i.e., deforestation, land-use conversion, habitat fragmentation, ecological level (i.e., ecosystem, community, population, genetic) and taxonomic group. Secondly, we summarize the impacts of habitat degradation on biodiversity and ecosystem services, and identify deficiencies in current knowledge. We show that habitat degradation in TMFs impacts biodiversity at all ecological levels and will be compounded by climate change. However, despite montane species being perceived as more extinction-prone due to their restricted geographic ranges, there are some indications of biotic resilience if the impacts to TMFs are less severe. Species richness and key species interactions can be maintained in mildly degraded sites, and gene flow can persist between TMF fragments. As such, minimally degraded areas such as secondary forests and restored areas could play a crucial role in maintaining the meta-community and ecosystem services of TMFs—either via resource provision or by linking patches of pristine forest. Research deficiencies highlighted include poor research representation in Asian and African TMFs, few assessments of population and genetic-level responses to fragmentation, and little assessment of the impacts of habitat fragmentation at all ecological levels. To address these concerns, we present a list of the top research priorities to urgently address the growing threat of habitat degradation in TMF

Impacts of Habitat Degradation on Tropical Montane Biodiversity and Ecosystem Services: A Systematic Map for Identifying Future Research Priorities

Tropical montane forests (TMFs) are major centers of evolutionary change and harbor many endemic species with small geographic ranges. In this systematic map, we focus on the impacts of anthropogenic habitat degradation on TMFs globally. We first determine how TMF research is distributed across geographic regions, degradation type (i.e., deforestation, land-use conversion, habitat fragmentation, ecological level (i.e., ecosystem, community, population, genetic) and taxonomic group. Secondly, we summarize the impacts of habitat degradation on biodiversity and ecosystem services, and identify deficiencies in current knowledge. We show that habitat degradation in TMFs impacts biodiversity at all ecological levels and will be compounded by climate change. However, despite montane species being perceived as more extinction-prone due to their restricted geographic ranges, there are some indications of biotic resilience if the impacts to TMFs are less severe. Species richness and key species interactions can be maintained in mildly degraded sites, and gene flow can persist between TMF fragments. As such, minimally degraded areas such as secondary forests and restored areas could play a crucial role in maintaining the meta-community and ecosystem services of TMFs—either via resource provision or by linking patches of pristine forest. Research deficiencies highlighted include poor research representation in Asian and African TMFs, few assessments of population and genetic-level responses to fragmentation, and little assessment of the impacts of habitat fragmentation at all ecological levels. To address these concerns, we present a list of the top research priorities to urgently address the growing threat of habitat degradation in TMF

A False Start in the Race Against Doping in Sport: Concerns With Cycling’s Biological Passport

Professional cycling has suffered from a number of doping scandals. The sport’s governing bodies have responded by implementing an aggressive new antidoping program known as the biological passport. Cycling’s biological passport marks a departure from traditional antidoping efforts, which have focused on directly detecting prohibited substances in a cyclist’s system. Instead, the biological passport tracks biological variables in a cyclist’s blood and urine over time, monitoring for fluctuations that are thought to indirectly reveal the effects of doping. Although this method of indirect detection is promising, it also raises serious legal and scientific concerns. Since its introduction, the cycling community has debated the reliability of indirect biological-passport evidence and the clarity, consistency, and transparency of its use in proving doping violations. Such uncertainty undermines the legitimacy of finding cyclists guilty of doping based on this indirect evidence alone. Antidoping authorities should address these important concerns before continuing to pursue doping sanctions against cyclists solely on the basis of their biological passports