Green coffee seed residue: A sustainable source of antioxidant compounds.

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A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations

A collaborative effort was carried out by the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) to promote knowledge exchange between associate laboratories interested in the implementation of indel-based methodologies and build allele frequency databases of 38 indels for forensic applications. These databases include populations from different countries that are relevant for identification and kinship investigations undertaken by the participating laboratories. Before compiling population data, participants were asked to type the 38 indels in blind samples from annual GHEP-ISFG proficiency tests, using an amplification protocol previously described. Only laboratories that reported correct results contributed with population data to this study. A total of 5839 samples were genotyped from 45 different populations from Africa, America, East Asia, Europe and Middle East. Population differentiation analysis showed significant differences between most populations studied from Africa and America, as well as between two Asian populations from China and East Timor. Low FST values were detected among most European populations. Overall diversities and parameters of forensic efficiency were high in populations from all continents.RP is supported by a postdoctoral fellowship (SFRH/BPD/81986/2011) awarded by the Portuguese Foundation for Science and Technology (FCT) and co-financed by the European Social Fund (Human Potential Thematic Operational Programme – POPH

A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations

A collaborative effort was carried out by the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) to promote knowledge exchange between associate laboratories interested in the implementation of indel-based methodologies and build allele frequency databases of 38 indels for forensic applications. These databases include populations from different countries that are relevant for identification and kinship investigations undertaken by the participating laboratories. Before compiling population data, participants were asked to type the 38 indels in blind samples from annual GHEP-ISFG proficiency tests, using an amplification protocol previously described. Only laboratories that reported correct results contributed with population data to this study. A total of 5839 samples were genotyped from 45 different populations from Africa, America, East Asia, Europe and Middle East. Population differentiation analysis showed significant differences between most populations studied from Africa and America, as well as between two Asian populations from China and East Timor. Low FST values were detected among most European populations. Overall diversities and parameters of forensic efficiency were high in populations from all continents.Instituto Multidisciplinario de Biología CelularFacultad de Ciencias Naturales y Muse

A second-step splicing activity is conserved from yeast to human

We describe a defective HeLa nuclear extract which is particularly deficient in step 2 of splicing reaction. With this extract we have studied the conservation of a second-step activity from yeast to human cells. We detected a S. cerevisiae second-step splicing activity that allows restoration of step 2 of the defective HeLa nuclear extract, which indicates that yeast purified fraction has a second-step activity that is conserved from yeast to human cells. The activity is a yeast UsnRNP protein(s) since it is purified with anti-tri-methylguanosine by immunoaffinity columns.This work was supported by PGC Grant No. PB92-0004 and an Alexander von Humboldt Foundation grant. C. Codony was the recipient of a fellowship from PGC; R.B. Cicarelli was the recipient from a fellowship from CAPES and Spanish MEC; A. Khaouja was the recipient of a fellowship from the Moroccan government.Peer Reviewe

Presence of autoantibodies against small nuclear ribonucleoprotein epitopes in Chagas' patients' sera

We investigated the possibility that Chagas' patients develop an autoimmune response to human UsnRNPs (small nuclear ribonucleoprotein) or Sm epitopes. Using purified human UsnRNPs, we detected anti-human UsnRNPs antibodies in sera from patients suffering from Chagas' disease. The antibodies were also detected using peptide enzyme-linked immunosorbent assays containing the Sm-motif 1 domain. The latter technique showed that 61% (31/51) of the Chagas' patients' sera contained antibodies against Sm-motif 1. The detection of anti-UsnRNPs autoantibodies in Chagas' patients' sera strongly encourages further studies using animal models to determine how these autoantibodies appear.This work was supported by PGC grant PB92-0004. R.B. Cicarelli was the recipient of fellowships from CAPES and Spanish MEC; D. Bahia was the recipient of a MUTIS fellowship.Peer Reviewe

Genetic population data of 12 STR loci of the PowerPlex (R) Y system in the state of Sao Paulo population (Southeast of Brazil)

Allele frequency distributions and population data for 12 Y-chromosomal short tandem repeats (STRs) included in the PowerPlex (R) Y Systems (Promega) were obtained for a sample of 200 healthy unrelated males living in S (a) over tildeo Paulo State (Southeast of Brazil). A total of 192 haplotypes were identified, of which 184 were unique and 8 were found in 2 individuals. The average gene diversity of the 12 Y-STR was 0.6746 and the haplotype diversity was 0.9996. Pairwise analysis confirmed that our population is more similar with the Italy, North Portugal and Spain, being more distant of the Japan. (c) 2007 Elsevier Ireland Ltd. All rights reserved

Antiprotozoal Sesquiterpene Pyridine Alkaloids from <i>Maytenus ilicifolia</i>

As part of a bioprospecting program aimed at the discovery of antiprotozoal agents from the Brazilian flora, two new sesquiterpene pyridine alkaloids, ilicifoliunines A (<b>1</b>) and B (<b>2</b>), along with the known alkaloids aquifoliunine E-I (<b>3</b>) and mayteine (<b>4</b>), were isolated from the root bark of <i>Maytenus ilicifolia</i>. The structures of <b>1</b> and <b>2</b> were established on the basis of spectroscopic data interpretation. Alkaloid <b>3</b> displayed potent in vitro antiprotozoal activity against <i>Leishmania chagasi</i> and <i>Trypanosoma cruzi</i>, with IC₅₀ values of 1.4 and 41.9 μM, respectively, as well as low cytotoxicity against murine peritoneal macrophages (IC₅₀ of 1.8 mM)

University marketing: perceptions, practices and prospects in the less developed world

The article reports on research conducted in Zimbabwe's 11 universities between 2001 and 2003. The research sought to find our VCs perceptions of marketing, and the extent to which university customers considered the current marketing arrangements to address their choice and decision needs in the diversifying HE market of Zimbabwe. The research used interviews and questionnaires as the basic data gathering approaches. It found a variety of marketing conceptualisations among the chief university executives and that there was a disjuncture between the core business of universities and their marketing orientations. The paper proposes a new curriculum focused model as a sound bais for developing the Marketing orientation in universities in Zimbabwe