Management and recovery of an urbanized catchment.

Divulga os resultados do mapeamento, utilizando geoprocessamento, da Bacia Hidrográfica de Servidão, em Rio Claro-S

Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans

The respiration, membrane potential (Dy), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial Dy respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 µM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 µM) inhibited respiration by 30% and 2 µM antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was partially blocked by 2 mM benzohydroxamic acid, suggesting the presence of an alternative oxidase. Candida albicans mitochondria in situ presented a carboxyatractyloside-insensitive increase of Dy induced by 5 mM ATP and 0.5% BSA, and Dy decrease induced by 10 µM linoleic acid, both suggesting the existence of an uncoupling protein. The presence of this protein was subsequently confirmed by immunodetection and respiration experiments with isolated mitochondria. In conclusion, Candida albicans ATCC 90028 possesses an alternative electron transfer chain and alternative oxidase, both absent in animal cells. These pathways can be exceptional targets for the design of new chemotherapeutic agents. Blockage of these respiratory pathways together with inhibition of the uncoupling protein (another potential target for drug design) could lead to increased production of reactive oxygen species, dysfunction of Candida mitochondria, and possibly to oxidative cell death.1455146

Propriedades do ASIC da siderurgia nacional e possibilidades da sua utilização em obra : uma perspectiva geomecânica

Para avaliar a viabilidade de utilização dos ASIC (Agregado Siderúrgico Inerte para Construção) produzidos pela Siderurgia Nacional, em obras geotécnicas, estudam-se em laboratório as suas propriedades mineralógicas, químicas, geométricas, físicas e mecânicas. Neste trabalho apresentam-se os resultados obtidos para as propriedades físicas e mecânicas, em particular a rigidez estudada através de um equipamento triaxial de precisão. Os resultados são comparados com os obtidos em materiais especificados para as camadas de base dos pavimentos, quer em Portugal, quer em França (agregados granítico 0/31,5 e calcário 0/19, respectivamente). Da comparação dos resultados conclui-se que os ASIC apresentam propriedades mecânicas superiores às dos agregados naturais. Estes resultados enfatizam que os ASIC nacionais podem ser utilizados nas infra-estruturas de transporte

Laboratory and field leaching tests for predicting the environmental impact of Portuguese steel slag

The use of waste in the construction of Portuguese public works is feasible if there are no risks for the environment. The materials that meet these requirements in Portugal are those admissible for landfills of inert waste, their classification being done on the basis of the comparison of the leaching values obtained in standard laboratory leach tests with the leaching limit values established in the Portuguese legislation. This paper addresses the results of the leaching tests performed in laboratory and in the field with materials coming from electrical arc furnace steel slag produced in the Portuguese Iron and Steel Company

Isolamento e seleção de rizóbios de solos de Mato Grosso Do Sul para inoculação em feijoeiro comum

bitstream/item/66207/1/32003.pdfFERTBI

Baixas temperaturas e risco de morte súbita cardíaca na esquizofrenia: desvendando um novo calcanhar de Aquiles?

Universidade Federal de São Paulo (UNIFESP) Laboratory of Experimental NeurologyUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of PhysiologyUniversidade de São Paulo School of Medicine Department and Institute of PsychiatryUNIFESP, Laboratory of Experimental NeurologyUNIFESP, EPM, Department of PhysiologySciEL

Respiration, Oxidative Phosphorylation, And Uncoupling Protein In Candida Albicans.

The respiration, membrane potential (Deltapsi), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 microM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial Deltapsi respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 microM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 microM) inhibited respiration by 30% and 2 micro M antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was partially blocked by 2 mM benzohydroxamic acid, suggesting the presence of an alternative oxidase. Candida albicans mitochondria in situ presented a carboxyatractyloside-insensitive increase of Deltapsi induced by 5 mM ATP and 0.5% BSA, and Deltapsi decrease induced by 10 microM linoleic acid, both suggesting the existence of an uncoupling protein. The presence of this protein was subsequently confirmed by immunodetection and respiration experiments with isolated mitochondria. In conclusion, Candida albicans ATCC 90028 possesses an alternative electron transfer chain and alternative oxidase, both absent in animal cells. These pathways can be exceptional targets for the design of new chemotherapeutic agents. Blockage of these respiratory pathways together with inhibition of the uncoupling protein (another potential target for drug design) could lead to increased production of reactive oxygen species, dysfunction of Candida mitochondria, and possibly to oxidative cell death.371455-6

Risk-assessment algorithm and recommendations for venous thromboembolism prophylaxis in medical patients

The risk for venous thromboembolism (VTE) in medical patients is high, but risk assessment is rarely performed because there is not yet a good method to identify candidates for prophylaxis

Thalamic nuclear abnormalities as a contributory factor in sudden cardiac deaths among patients with schizophrenia

Patients with schizophrenia have a two- to three-fold increased risk of premature death as compared to patients without this disease. It has been established that patients with schizophrenia are at a high risk of developing cardiovascular disease. Moreover, an important issue that has not yet been explored is a possible existence of a “cerebral” focus that could trigger sudden cardiac death in patients with schizophrenia. Along these lines, several structural and functional alterations in the thalamic complex are evident in patients with schizophrenia and have been correlated with the symptoms manifested by these patients. With regard to abnormalities on the cellular and molecular level, previous studies have shown that schizophrenic patients have fewer neuronal projections from the thalamus to the prefrontal cortex as well as a reduced number of neurons, a reduced volume of either the entire thalamus or its subnuclei, and abnormal glutamate signaling. According to the glutamate hypothesis of schizophrenia, hypofunctional corticostriatal and striatothalamic projections are directly involved in the pathophysiology of the disease. Animal and post-mortem studies have provided a large amount of evidence that links the sudden unexpected death in epilepsy (SUDEP) that occurs in patients with schizophrenia and epilepsy to thalamic changes. Based on the results of these prior studies, it is clear that further research regarding the relationship between the thalamus and sudden cardiac death is of vital importance