Clinical changes in cats submitted to experimental hyperthyroidism

A relação do hipertireoidismo experimental com parâmetros clínicos foi avaliada em gatos machos e fêmeas, intactos e mantidos em estado hipertireóideo por um período de 42 dias. Os parâmetros estudados foram consumo diário de água e ração, débito urinário, o volume fecal, peso corporal, temperatura corporal, coloração de membranas mucosas, palpação de linfonodos superficiais, palpação da glândula tireóide, pulso femoral, auscultação cardíaca e respiratória, freqüência cardíaca e respiratória e palpação abdominal. Correlacionou-se as variáveis com os níveis de T3 total, T4 livre e total. O excesso dos hormônios tireoidianos acompanhou aumento da freqüência cardíaca, polifagia, polidipsia, perda de peso e aumento do volume fecal nos gatos estudados.The interrelation between experimental hyperthyroidism andclinical changes was studied in adult intact cats kept under hyperthyroidic state for 42 days. Clinical aspects evaluated were dairy food and water intake, urine output and fecal volume, body weight, temperature, mucous membranes colour, palpable lymphnodes, thyroid gland palpation, abdominal palpation, and cardiac and respiratory frequencies. These characteristics were correlated with free and total T4 and total T3 serum concentrations. Excessive circulant thyroid hormone levels resulted in increased cardiac frequency, polyuria, polidipsia, weight loss and increased fecal volume in the studied cats

Visual or spatial isolation in ruminants.

Livestock production in intensive systems has expanded the world, always agreeing to raise production levels and economic returns, respectively. This vertical integration of livestock has led to some adjustments in management and also on the premises, which has changed the behavior of animals, coming from both visual and spatial constraints of the individual. In dairy farming, the creation of calves in individual stalls is an example of vertical integration, thus making a common practice in many countries. Knowing that the cattle are social animals that show high willingness to form social bonds with other individuals of the same species, some agencies have implemented laws to ensure a minimum of comfort for these animals. A systematic study of social preferences of animals and the calming effects of different animal companion familiarity are needed to document the response to separation. The objective of this review, present and discuss the effect of spatial isolation and/or visual on the productive and behavioral responses of ruminant animals.A produção animal em sistemas intensivos tem se ampliado no mundo, sempre visando à elevação de índices produtivos e retorno econômico, respectivamente. Essa verticalização da pecuária tem provocado algumas adaptações de manejo e também nas instalações, que tem alterado o comportamento dos animais, oriundo das restrições tanto visual como espacial do indivíduo. Na pecuária leiteira, a criação de bezerros em baias individuais é um exemplo dessa verticalização, tornando então uma prática comum em muitos países. Sabendo que os bovinos são animais sociais que mostram alta disponibilidade para formar vínculo social com outros indivíduos da mesma espécie, alguns órgãos têm implantado legislações para garantir um mínimo de conforto para esses animais. Um estudo sistemático das preferências sociais dos animais e os efeitos calmantes do animal companheiro de familiaridade diferente são necessários para documentar a resposta à separação. Objetivou-se com esta revisão, apresentar e discutir o efeito do isolamento espacial e/ou visual sobre as respostas produtivas e comportamentais de animais ruminantes

Targeted massively parallel sequencing panel to diagnose genetic endocrine disorders in a tertiary hospital

Objectives: To analyze the efficiency of a multigenic targeted massively parallel sequencing panel related to endocrine disorders for molecular diagnosis of patients assisted in a tertiary hospital involved in the training of medical faculty. Material and methods: Retrospective analysis of the clinical diagnosis and genotype obtained from 272 patients in the Endocrine unit of a tertiary hospital was performed using a custom panel designed with 653 genes, most of them already associated with the phenotype (OMIM) and some candidate genes that englobes developmental, metabolic and adrenal diseases. The enriched DNA libraries were sequenced in NextSeq 500. Variants found were then classified according to ACMG/AMP criteria, with Varsome and InterVar. Results: Three runs were performed; the mean coverage depth of the targeted regions in panel sequencing data was 249×, with at least 96.3% of the sequenced bases being covered more than 20-fold. The authors identified 66 LP/P variants (24%) and 27 VUS (10%). Considering the solved cases, 49 have developmental diseases, 12 have metabolic and 5 have adrenal diseases. Conclusion: The application of a multigenic panel aids the training of medical faculty in an academic hospital by showing the picture of the molecular pathways behind each disorder. This may be particularly helpful in developmental disease cases. A precise genetic etiology provides an improvement in understanding the disease, guides decisions about prevention or treatment, and allows genetic counseling