Participação em uma pesquisa para avaliar os ciudados primários da saúde entre filiados e filiadas de um plano médico universitário na Argentina

Objetivo: Describir la proporción de localización, participación y respuesta en una encuesta para valorar la atención primaria de la salud de afiliados de una obra social universitaria. Métodos: Estudio piloto descriptivo transversal. Muestreo aleatorio simple de 500 personas adultas de la base de datos de afiliaciones. Se calcularon proporciones de localización, participación, respuesta y respuesta total, luego se analizaron por sexo, edad, tipo de afiliación y modo de administración. Resultados: El 61,8% de afiliados fue localizado, el 84,5% de las personas localizadas aceptó participar y el 64,4% de ellas respondió el cuestionario, alcanzando una proporción de respuesta total del 54,4%. Las personas jóvenes fueron relativamente menos localizadas (52,2%; p<0,01) y la mayor proporción de respuesta total se consiguió en las mayores de 65 años (68,2%; p<0,05). Conclusiones: La localización fue dificultosa pero hubo una buena aceptación del estudio. Los medios informatizados de administración fueron preferidos pero tuvieron una baja respuesta.Aim: To describe the location, participation and response to a survey to assess primary health care in members of a University Health Insurance. Materials and methods: Pilot cross-sectional descriptive study. Simple random sample of 500 adults from members’ database. Proportions of location, participation, response and total response were calculated and analyzed according to sex, age, and type of membership and administration. Results: 61.8% of members were found, 84.5% agreed to participate and 64.4% answered the questionnaire, reaching a total response rate of 54.4%. Young people were less likely to be localized (52.2%, P <0.01) and higher total response rate was achieved in people over 65 (68.2%, P <0.05). Conclusions: Finding the members was difficult but there was a good level of study acceptance. The computerized questionnaire showed strong preference but low response.Objetivo: Descrever a proporção de localização, participação e resposta em uma enquete para avaliar os cuidados de saúde primários dos filiados de um plano médico universitário. Métodos: Estudo piloto descritivo transversal. Amostragem aleatória simples de 500 adultos do banco de dados das filiações. Proporções de localização, a participação, a resposta e a resposta global foram apurados; a seguir foram analisados por sexo, idade, tipo de filiação e modo de administração. Resultados: 61,8 % dos filiados foi localizado, 84,5 % daqueles localizados aceitaram participar e 64,4% deles responderam o questionário, atingindo uma taxa de resposta total de 54,4%. Os jovens foram relativamente menos localizados (52,2%; p <0,01 ) e a maior proporção de resposta completa foi atingida em pessoas com mais de 65 anos (68,2% , p <0,05). Conclusões: A localização foi difícil, mas houve uma boa aceitação da pesquisa. Os meios de administração informatizados foram os preferidos, mas tiveram uma resposta fraca.Fil: Villalba, A.. Universidad Nacional de Cordoba. Facultad de Derecho y Cs.sociales. Escuela de Trabajo Social; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Escuela de Salud Pública; ArgentinaFil: Falá Giroldi, C.. Universidad Nacional de Cordoba. Facultad de Derecho y Cs.sociales. Escuela de Trabajo Social; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Escuela de Salud Pública; ArgentinaFil: Nicora, V.. Universidad Nacional de Cordoba. Facultad de Derecho y Cs.sociales. Escuela de Trabajo Social; ArgentinaFil: Mántaras, J.. Universidad Nacional de Cordoba. Facultad de Derecho y Cs.sociales. Escuela de Trabajo Social; ArgentinaFil: Berra, Silvina del Valle. Universidad Nacional de Córdoba. Facultad de Medicina. Escuela de Salud Pública; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Functional diversification of maize RNA polymerase IV and V subtypes via alternative catalytic subunits.

Made available in DSpace on 2018-06-26T01:00:59Z (GMT). No. of bitstreams: 1 1s2.0S2211124714007591main.pdf: 2595666 bytes, checksum: e310930ff02eb5cd88dc9c632378b987 (MD5) Previous issue date: 2015-01-14bitstream/item/115427/1/1-s2.0-S2211124714007591-main.pd

Evidence supporting dissimilatory and assimilatory lignin degradation in Enterobacter lignolyticus SCF1

Lignocellulosic biofuels are promising as sustainable alternative fuels, but lignin inhibits access of enzymes to cellulose, and by-products of lignin degradation can be toxic to cells. The fast growth, high efficiency and specificity of enzymes employed in the anaerobic litter deconstruction carried out by tropical soil bacteria make these organisms useful templates for improving biofuel production. The facultative anaerobe Enterobacter lignolyticus SCF1 was initially cultivated from Cloud Forest soils in the Luquillo Experimental Forest in Puerto Rico, based on anaerobic growth on lignin as sole carbon source. The source of the isolate was tropical forest soils that decompose litter rapidly with low and fluctuating redox potentials, where bacteria using oxygen-independent enzymes likely play an important role in decomposition. We have used transcriptomics and proteomics to examine the observed increased growth of SCF1 grown on media amended with lignin compared to unamended growth. Proteomics suggested accelerated xylose uptake and metabolism under lignin-amended growth, with up-regulation of proteins involved in lignin degradation via the 4-hydroxyphenylacetate degradation pathway, catalase/peroxidase enzymes, and the glutathione biosynthesis and glutathione S-transferase (GST) proteins. We also observed increased production of NADH-quinone oxidoreductase, other electron transport chain proteins, and ATP synthase and ATP-binding cassette (ABC) transporters. This suggested the use of lignin as terminal electron acceptor. We detected significant lignin degradation over time by absorbance, and also used metabolomics to demonstrate moderately significant decreased xylose concentrations as well as increased metabolic products acetate and formate in stationary phase in lignin-amended compared to unamended growth conditions. Our data show the advantages of a multi-omics approach toward providing insights as to how lignin may be used in nature by microorganisms coping with poor carbon availability

Bio-chronostratigraphic calibration of the Upper Carnian-Lower Norian magnetostratigraphic scale at Pizzo Mondello (Sicani Mountains, Sicily).

Pizzo Mondello section is known since 15 years because of the continuous Late Triassic pelagic record of great significance for the establishment of an integrated chronostratigraphy of the Late Triassic (Gullo et al. 1996; Muttoni et al. 2001, 2004). During the last 4 years, Pizzo Mondello section has been studied in detail to provide a new and high resolution integrated bio-chronostratigraphy for the calibration of the magnetostratigraphy and chemostratigraphy proposed by Muttoni et al. (2001, 2004), and now it is one of the GSSP candidates for the definition of the base of the Norian. The lowest 143 m of the Cherty Limestone, straddling the C/N boundary have been studied in detail. The preliminary data of the ongoing research have been presented in all the meetings of the STS from Albuquerque 2007 and here we summarize the final results. The key correlation to the standard marine Triassic Scale is provided by the ammonoids. They are relatively rare, however the available collections document the Upper Carnian Discotropites plinii and Gonionotites italicus Subzones, from meter 15 to meter 80 from the base of the section. The following 15 meters are poor in ammonoids, while higher up the lower part of the Lower Norian Guembelites jandianus Zone is documented by Dimorphites cf. n. sp.1 of Krystyn, 1980. Conodonts are very abundant and have a great potential as practical tool for global correlations. The abundance of specimens at Pizzo Mondello gave the opportunity to point out clear relationships among the five most widespread Upper Camian/Lower Norian conodont genera (Paragondolella, Carnepigondolella, Metapolygnathus, Epigondolella and Norigondolella) and to identify trends of the generic turnovers (Mazza et al. 2010). The two biomarkers so far proposed as possible marker events for the GSSP were the FAD of E. quadrata (sample FNP88A) and the FAD of M. communisti (sample NA35). However, the FAD of E. quadrata occurs within the Gonionotites italicus Subzone, while the FAD of M. communisti is on its top. Halobiids are extremely common in the Cherty Limestone and they have also a great potential for large scale correlations. Nine species of Halobia have been recognized: Halobia carnica, H. lenticularis, H. simplex, H. superba, H. cf. rugosa, H. radiata, H. austriaca, H. styriaca and H. mediterranea. The best possible marker for the base of the Norian is the first occurrence of Halobia austriaca, that is recorded in the middle of the interval between the record of the Gonionotites italicus Subzone and the Guembelites jandianus Zone. Radiolarians were found in few samples but with high diversity assemblages. In the upper Gonionotites italicus Subzone to the Guembelites jandianus Zone there is an overlap of species previously considered Late Carnian with species usually regarded as Early Norian. About 4 m above the FAD of E. quadrata, in the Gonionotites italicus Subzone, the first assemblage with Capnuchosphaera deweveri Kozur & Mostler, Capnuchosphaera tricornis De Wever, Kahlerosphaera norica Kozur & Mock and Xiphothecaella longa Kozur & Mock, usually referred to Early Norian, occurs. These integrated bio-chronostratigraphic studies lead to identify some possible GSSP marker events especially on conodonts and halobiids, which occur in the upper part of magnetozone PM 4n, within PM 4r and in the lower part of PM 5n. Possibly the most suitable magnetostratigraphic event to recognize the basal Norian is the base of magnetozone PM 5n, as already suggested by Krystyn et al. 2002 and Muttoni et al. 2004

MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling

The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host–pathogen relationships and facilitate the development of new therapies and vaccines

Clinical characteristics of coronavirus disease (COVID-19) early findings from a teaching hospital in Pavia, North Italy, 21 to 28 February 2020

We describe clinical characteristics, treatments and outcomes of 44 Caucasian patients with coronavirus disease (COVID-19) at a single hospital in Pavia, Italy, from 21\u201328 February 2020, at the beginning of the outbreak in Europe. Seventeen patients developed severe disease, two died. After a median of 6 days, 14 patients were discharged from hospital. Predictors of lower odds of discharge were age&gt;65 years, antiviral treatment and for severe disease, lactate dehydrogenase &gt;300 mg/dL

Expanding tropical forest monitoring into Dry Forests: The DRYFLOR protocol for permanent plots

This is the final version. Available on open access from Wiley via the DOI in this recordSocietal Impact Statement Understanding of tropical forests has been revolutionized by monitoring in permanent plots. Data from global plot networks have transformed our knowledge of forests’ diversity, function, contribution to global biogeochemical cycles, and sensitivity to climate change. Monitoring has thus far been concentrated in rain forests. Despite increasing appreciation of their threatened status, biodiversity, and importance to the global carbon cycle, monitoring in tropical dry forests is still in its infancy. We provide a protocol for permanent monitoring plots in tropical dry forests. Expanding monitoring into dry biomes is critical for overcoming the linked challenges of climate change, land use change, and the biodiversity crisis.Newton FundNatural Environment Research Council (NERC)Fundação de Amparo à Pesquisa do Estado de São PauloCYTE

A Search for Photons with Energies Above 2 × 1017^{17} eV Using Hybrid Data from the Low-Energy Extensions of the Pierre Auger Observatory

Ultra-high-energy photons with energies exceeding 10$^{17}$ eV offer a wealth of connections to different aspects of cosmic-ray astrophysics as well as to gamma-ray and neutrino astronomy. The recent observations of photons with energies in the 10$^{15}$ eV range further motivate searches for even higher-energy photons. In this paper, we present a search for photons with energies exceeding 2 × 10$^{17}$ eV using about 5.5 yr of hybrid data from the low-energy extensions of the Pierre Auger Observatory. The upper limits on the integral photon flux derived here are the most stringent ones to date in the energy region between 10$^{17}$ and 10$^{18}$ eV