22,947 research outputs found
Octopamine increases the excitability of neurons in the snail feeding system by modulation of inward sodium current but not outward potassium currents
Background: Although octopamine has long been known to have major roles as both transmitter and modulator in arthropods, it has only recently been shown to be functionally important in molluscs, playing a role as a neurotransmitter in the feeding network of the snail Lymnaea stagnalis. The synaptic potentials cannot explain all the effects of octopamine-containing neurons on the feeding network, and here we test the hypothesis that octopamine is also a neuromodulator. Results: The excitability of the B1 and B4 motoneurons in the buccal ganglia to depolarising current clamp pulses is significantly (P << 0.05) increased by (10 mu M) octopamine, whereas the B2 motoneuron becomes significantly less excitable. The ionic currents evoked by voltage steps were recorded using 2-electrode voltage clamp. The outward current of B1, B2 and B4 motoneurons had two components, a transient I-A current and a sustained I-K delayed-rectifier current, but neither was modulated by octopamine in any of these three buccal neurons. The fast inward current was eliminated in sodium - free saline and so is likely to be carried by sodium ions. 10 mu M octopamine enhanced this current by 33 and 45% in the B1 and B4 motoneurons respectively (P << 0.05), but a small reduction was seen in the B2 neuron. A Hodgkin-Huxley style simulation of the B1 motoneuron confirms that a 33% increase in the fast inward current by octopamine increases the excitability markedly. Conclusion: We conclude that octopamine is also a neuromodulator in snails, changing the excitability of the buccal neurons. This is supported by the close relationship from the voltage clamp data, through the quantitative simulation, to the action potential threshold, changing the properties of neurons in a rhythmic network. The increase in inward sodium current provides an explanation for the polycyclic modulation of the feeding system by the octopamine-containing interneurons, making feeding easier to initiate and making the feeding bursts more intense
Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 Å resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses
The ordered K-theory of a full extension
Let A be a C*-algebra with real rank zero which has the stable weak
cancellation property. Let I be an ideal of A such that I is stable and
satisfies the corona factorization property. We prove that 0->I->A->A/I->0 is a
full extension if and only if the extension is stenotic and K-lexicographic. As
an immediate application, we extend the classification result for graph
C*-algebras obtained by Tomforde and the first named author to the general
non-unital case. In combination with recent results by Katsura, Tomforde, West
and the first author, our result may also be used to give a purely
K-theoretical description of when an essential extension of two simple and
stable graph C*-algebras is again a graph C*-algebra.Comment: Version IV: No changes to the text. We only report that Theorem 4.9
is not correct as stated. See arXiv:1505.05951 for more details. Since
Theorem 4.9 is an application to the main results of the paper, the main
results of this paper are not affected by the error. Version III comments:
Some typos and errors corrected. Some references adde
Search for associations containing young stars (SACY). V. Is multiplicity universal? Tight multiple systems
Context: Dynamically undisrupted, young populations of stars are crucial to
study the role of multiplicity in relation to star formation. Loose nearby
associations provide us with a great sample of close (150 pc) Pre-Main
Sequence (PMS) stars across the very important age range (5-70 Myr) to
conduct such research.
Aims: We characterize the short period multiplicity fraction of the SACY
(Search for Associations Containing Young stars) accounting for any
identifiable bias in our techniques and present the role of multiplicity
fractions of the SACY sample in the context of star formation.
Methods: Using the cross-correlation technique we identified double-lined
spectroscopic systems (SB2), in addition to this we computed Radial Velocity
(RV) values for our subsample of SACY targets using several epochs of FEROS and
UVES data. These values were used to revise the membership of each association
then combined with archival data to determine significant RV variations across
different data epochs characteristic of multiplicity; single-lined multiple
systems (SB1).
Results: We identified 7 new multiple systems (SB1s: 5, SB2s: 2). We find no
significant difference between the short period multiplicity fraction
() of the SACY sample and that of nearby star forming regions
(1-2 Myr) and the field (10%) both as a function of
age and as a function of primary mass, , in the ranges [1:200 day] and
[0.08 -].
Conclusions: Our results are consistent with the picture of universal star
formation, when compared to the field and nearby star forming regions (SFRs).
We comment on the implications of the relationship between increasing
multiplicity fraction with primary mass, within the close companion range, in
relation to star formation.Comment: 14 pages, 18 figures, published, A&A
http://dx.doi.org/10.1051/0004-6361/20142385
Prevalence of Anderson-Fabry disease in male patients with late onset hypertrophic cardiomyopathy
Background-Although studies have suggested that "late-onset" hypertrophic cardiomyopathy (HCM) may be caused by sarcomeric protein gene mutations, the cause of HCM in the majority of patients is unknown. This study determined the prevalence of a potentially treatable cause of hypertrophy, Anderson-Fabry disease, in a HCM referral population.Methods and Results-Plasma alpha-galactosidase A (alpha-Gal) was measured in 79 men with HCM who were diagnosed at greater than or equal to40 years of age (52.9 +/- 7.7 years; range, 40-71 years) and in 74 men who were diagnosed at <40 years (25.9 +/- 9.2 years; range, 8-39 years). Five patients (6.3%) with late-onset disease and 1 patient (1.4%) diagnosed at <40 years had low alpha-Gal activity. Of these 6 patients, 3 had angina, 4 were in New York Heart Association class 2, 5 had palpitations, and 2 had a history of syncope. Hypertrophy was concentric in 5 patients and asymmetric in 1 patient. One patient had left ventricular outflow tract obstruction. All patients with low alpha-Gal activity had alpha-Gal gene mutations.Conclusion-Anderson-Fabry disease should be considered in all cases of unexplained hypertrophy. Its recognition is important given the advent of specific replacement enzyme therapy
Modelling cell motility and chemotaxis with evolving surface finite elements
We present a mathematical and a computational framework for the modelling of cell motility. The cell membrane is represented by an evolving surface, with the movement of the cell determined by the interaction of various forces that act normal to the surface. We consider external forces such as those that may arise owing to inhomogeneities in the medium and a pressure that constrains the enclosed volume, as well as internal forces that arise from the reaction of the cells' surface to stretching and bending. We also consider a protrusive force associated with a reaction-diffusion system (RDS) posed on the cell membrane, with cell polarization modelled by this surface RDS. The computational method is based on an evolving surface finite-element method. The general method can account for the large deformations that arise in cell motility and allows the simulation of cell migration in three dimensions. We illustrate applications of the proposed modelling framework and numerical method by reporting on numerical simulations of a model for eukaryotic chemotaxis and a model for the persistent movement of keratocytes in two and three space dimensions. Movies of the simulated cells can be obtained from http://homepages.warwick.ac.uk/maskae/CV_Warwick/Chemotaxis.html
Paspalum grass
Originally native to Uruguay and Argentina, Paspalum dilatatum was introduced into the U.S.A. about the middle of the 19th century and is now firmly established and cultivated in the Gulf States where it is known as Dallis erass after A. T. Dallis of La Grange, Georgia. It was introduced into Australia by Baron von Mueller about 1880 and since 1898 its cultivation has steadily increased
Some Problems in Liquidating Personal Holding Companies
Ordinarily, distributions by a personal holding company qualify for the dividends paid deduction only if they are dividends under section 316. However, certain distributions in liquidation may also qualify. These liquidating distributions of a personal holding company are divided into two categories: (1) Distributions to the extent of earnings and profits for the taxable year (computed without regard to capital losses) made in complete liquidation of the corporation occurring within 24 months after the adoption of the plan of liquidation; and (2) distributions in liquidation properly chargeable to earnings and profits accumulated after February 28, 1913
Elephant Grass
Elephant Grass, sometimes known as Napier\u27s Fodder, is a hardy, palatable and nutritious plant which under good conditions will give heavy yields. When once established it will remain as a permanent stand so long as it receives good treatment. It grows well in many parts of Western Australia and up to date appears to be free from attack by pests and diseases.
* (Revised from Leaflet No. 600, by H. G. Elliott
Exact solution of a many-fermion system and its associated boson field
Journal ArticleLuttinger's exactly soluble model of a one-dimensional many-fermion system is discussed. We show that he did not solve his model properly because of the paradoxical fact that the density operator commutators [p(p), p(-p')], which always vanish for any finite number of particles, no longer vanish in the field-theoretic limit of a filled Dirac sea. In fact the operators p(p) define a boson field which is ipso facto associated with the Fermi-Dirac field
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