Morphological response to a North Sea bed depression induced by gas mining

Gas mining leads to saucer-like surface depressions. In the North Sea, gas is currently mined at several offshore locations. The associated bed depression has a similar spatial extent as offshore tidal sandbanks, which are large-scale bed patterns covering a significant part of the North Sea bottom. The morphological time scales of bed depressions and tidal sandbanks are similar, so that significant interaction between these features is expected. In this paper we allow the bed depression to become morphologically active. A simple depression model based on a homogeneous soil is tuned with data of a bed depression near the Dutch barrier island of Ameland. Next, this subsidence model is included in a morphodynamic model. We show that this model is able to explain tidal sandbanks, which represent natural bed behavior. Here we approximate the solution by an expansion up to first order. The zeroth-order solution of the model is a flat bed with a spatially uniform, time-independent current. The first-order solution is investigated using a Fourier transformation. In general, we observe significant interaction between the bed depression and the natural sandbank formation process. The process of induced bed depression triggers and intensifies the natural morphological behavior of the offshore seabed. The model also shows essential differences between modeling a morphodynamically active marine bottom depression and a bottom depression below the threshold for sediment motion. The maximum bed level depression in the active case is significantly larger, and the circular shape of depression contours is affected by stretching toward the preferred orientation of the tidal sandbank formation process

Характеристика структурних змін в яєчку при хронічній гіпертермії

Воздействие хронической гипертермии на яички экспериментальных животных приводит к изменению микроциркуляторного русла в виде венозного полнокровия и интерстициального отека. Появляются морфологические признаки замедления процессов сперматогенеза, которые сопровождаются изменениями структур извитых семенных канальцев.Laboratory animal testes influenced by chronic hyperthermia show changes in hemomicrocirculation bed structure manifested as venous hyperemia and interstitial edema. Morphological signs of spermatogenesis slowdown accompanied by changes in convoluted somniferous tubules are observed

Visual Ontology Cleaning: Cognitive Principles and Applicability

In this paper we connect two research areas, the Qualitative Spatial Reasoning and visual reasoning on ontologies. We discuss the logical limitations of the mereotopological approach to the visual ontology cleaning, from the point of view of its formal support. The analysis is based on three different spatial interpretations wich are based in turn on three different spatial interpretations of the concepts of an ontology.Ministerio de Educación y Ciencia TIN2004-0388

Enterobacter cloacae Outbreak and Emergence of Quinolone Resistance Gene in Dutch Hospital

Plasmid-mediated qnrA1 is an emerging resistance trait

Evolution in Quantum Leaps: Multiple Combinatorial Transfers of HPI and Other Genetic Modules in Enterobacteriaceae

Horizontal gene transfer is a key step in the evolution of Enterobacteriaceae. By acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. In Enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages, genomic islands (GIs) and genomic modules (GMs). The High Pathogenicity Island (HPI) is a GI encoding virulence genes that can be transferred between different Enterobacteriaceae. We investigated the HPI because it was present in an Enterobacter hormaechei outbreak strain (EHOS). Genome sequence analysis showed that the EHOS contained an integration site for mobile elements and harbored two GIs and three putative GMs, including a new variant of the HPI (HPI-ICEEh1). We demonstrate, for the first time, that combinatorial transfers of GIs and GMs between Enterobacter cloacae complex isolates must have occurred. Furthermore, the excision and circularization of several combinations of the GIs and GMs was demonstrated. Because of its flexibility, the multiple integration site of mobile DNA can be considered an integration hotspot (IHS) that increases the genomic plasticity of the bacterium. Multiple combinatorial transfers of diverse combinations of the HPI and other genomic elements among Enterobacteriaceae may accelerate the generation of new pathogenic strains

Confocal laser scanning microscopy analysis of S. epidermidis biofilms exposed to farnesol, vancomycin and rifampicin

Staphylococcus epidermidis is the major bacterial species found in biofilm-related infections on indwelling medical devices. Microbial biofilms are communities of bacteria adhered to a surface and surrounded by an extracellular polymeric matrix. Biofilms have been associated with increased antibiotic tolerance to the immune system. This increased resistance to conventional antibiotic therapy has lead to the search for new antimicrobial therapeutical agents. Farnesol, a quorum-sensing molecule in Candida albicans, has been described as impairing growth of several different microorganisms and we have previously shown its potential as an adjuvant in antimicrobial therapy against S. epidermidis. However, its mechanism of action in S. epidermidis is not fully known. In this work we better elucidate the role of farnesol against S: epidermidis biofilms using confocal laser scanning microscopy (CLSM). Findings 24 h biofilms were exposed to farnesol, vancomycin or rifampicin and were analysed by CLSM, after stained with a Live/Dead stain, a known indicator of cell viability, related with cell membrane integrity. Biofilms were also disrupted by sonication and viable and cultivable cells were quantified by colony forming units (CFU) plating. Farnesol showed a similar effect as vancomycin, both causing little reduction of cell viability but at the same time inducing significant changes in the biofilm structure. On the other hand, rifampicin showed a distinct action in S. epidermidis biofilms, by killing a significant proportion of biofilm bacteria. Conclusions While farnesol is not very efficient at killing biofilm bacteria, it damages cell membrane, as determined by the live/dead staining, in a similar way as vancomycin.. Furthermore, farnesol might induce biofilm detachment, as determined by the reduced biofilm biomass, which can partially explain the previous findings regarding its role as a possible chemotherapy adjuvant.(undefined

The commensal infant gut meta-mobilome as a potential reservoir for persistent multidrug resistance integrons

Despite the accumulating knowledge on the development and establishment of the gut microbiota, its role as a reservoir for multidrug resistance is not well understood. This study investigated the prevalence and persistence patterns of an integrase gene (int1), used as a proxy for integrons (which often carry multiple antimicrobial resistance genes), in the fecal microbiota of 147 mothers and their children sampled longitudinally from birth to 2 years. The study showed the int1 gene was detected in 15% of the study population, and apparently more persistent than the microbial community structure itself. We found int1 to be persistent throughout the first two years of life, as well as between mothers and their 2-year-old children. Metagenome sequencing revealed integrons in the gut meta-mobilome that were associated with plasmids and multidrug resistance. In conclusion, the persistent nature of integrons in the infant gut microbiota makes it a potential reservoir of mobile multidrug resistance