Воздействие хронической гипертермии на яички экспериментальных животных 

приводит к изменению микроциркуляторного русла в виде венозного полнокровия 



и интерстициального отека. Появляются морфологические признаки замедления 



процессов сперматогенеза, которые сопровождаются изменениями структур 



извитых семенных канальцев.Laboratory animal testes influenced by chronic hyperthermia show changes in 



hemomicrocirculation bed structure manifested as venous hyperemia and interstitial 



edema. Morphological signs of spermatogenesis slowdown accompanied by changes in 



convoluted somniferous tubules are observed

de Bruin, Alain

Duim, Birgitta

Fluit, Ad C

Glorieux, Sarah

Tulinski, Pawel

van Putten, Jos PM

Wagenaar, Jaap A

PubMed

Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 36108 CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcin

Pawel Tulinski

Ad C. Fluit

Jos P. M. Van Putten

Alain De Bruin

Sarah Glorieux

Jaap A. Wagenaar

Birgitta Duim

CiteSeerX

An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization

Tulinski, P.

Fluit, A.C.

van Putten, J.P.M.

de Bruin, A.

Glorieux, S.

Wagenaar, J.A.

Duim, B.

Utrecht University Repository

Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3×108 CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue

Fluit, Ad C.

van Putten, Jos P.M.

Wagenaar, Jaap A.

NARCIS 

Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3x10(8) CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue

Ghent University Academic Bibliography

English

An ex vivo porcine nasal mucosa explants model to study MRSA colonization

Advances in Veterinary Medicine

Strategic Infection Biology

Tissue Repair

Dep Infectieziekten Immunologie

Faculteit Diergeneeskunde

LS Infectiebiologie (Bacteriologie)

I&I SIB2

Dep Pathobiologie

Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3×10(8) CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue

Ad C Fluit

Jos P M van Putten

Alain de Bruin

Jaap A Wagenaar

Directory of Open Access Journals

PLoS ONE

An ex vivo porcine nasal mucosa explants model to study MRSA colonization.

Jos P. M. van Putten

Crossref

<div>Staphylococcus aureus is an opportunistic pathogen able to colonize the upper respiratory tract and skin surfaces in mammals. Methicillin-resistant S. aureus ST398 is prevalent in pigs in Europe and North America. However, the mechanism of successful pig colonization by MRSA ST398 is poorly understood. To study MRSA colonization in pigs, an ex vivo model consisting of porcine nasal mucosa explants cultured at an air-liquid interface was evaluated. In cultured mucosa explants from the surfaces of the ventral turbinates and septum of the pig nose no changes in cell morphology and viability were observed up to 72 h. MRSA colonization on the explants was evaluated followed for three MRSA ST398 isolates for 180 minutes. The explants were incubated with 3×108 CFU/ml in PBS for 2 h to allow bacteria to adhere to the explants surface. Next the explants were washed and in the first 30 minutes post adhering time, a decline in the number of CFU was observed for all MRSA. Subsequently, the isolates showed either: bacterial growth, no growth, or a further reduction in bacterial numbers. The MRSA were either localized as clusters between the cilia or as single bacteria on the cilia surface. No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue. </div

Pawel Tulinski (103903)

Ad C. Fluit (103909)

Jos P. M. van Putten (103913)

Alain de Bruin (103917)

Sarah Glorieux (103920)

Jaap A. Wagenaar (103922)

Birgitta Duim (103926)

The Francis Crick Institute

An Ex Vivo Porcine Nasal Mucosa Explants Model to Study MRSA Colonization

A human respiratory-tissue organ culture incorporating an air interface.

Adherence of Staphylococcus aureus to cell monolayers.

Bacterial and host factors implicated in nasal carriage of methicillin-resistant Staphylococcus aureus in mice.

Colonization and transmission of methicillin-resistant Staphylococcus aureus ST398 in nursery piglets.

Development and characterization of a Staphylococcus aureus nasal colonization model in mice.

Endocarditis due to meticillin-resistant Staphylococcus aureus originating from pigs.

Experimental colonization of pigs with methicillin-resistant Staphylococcus aureus (MRSA): Insights into the colonization and transmission of livestock-associated MRSA.

In vitro culture of porcine respiratory nasal mucosa explants for studying the interaction of porcine viruses with the respiratory tract.

Livestock-associated methicillin-resistant Staphylococcus aureus in humans,

Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans,

Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans.

Methicillin-resistant Staphylococcus aureus in people living and working in pig farms.

Methicillin-resistant Staphylococcus aureus in pig farming.

Murine nasal septa for respiratory epithelial air-liquid interface cultures.

Persistence of livestock associated MRSA CC398 in humans is dependent on intensity of animal contact.

Quantification of transmission of livestock-associated methicillin resistant Staphylococcus aureus in pigs.

Regulatory adaptation of Staphylococcus aureus during nasal colonization of humans.

The cotton rat as a model for Staphylococcus aureus nasal colonization in humans: Cotton rat S. aureus nasal colonization model.

The emergence of Staphylococcus aureus ST398. Vector Borne Zoonotic Dis

The role of nasal carriage in Staphylococcus aureus infections.

The Staphylococcus aureus surface protein SasG and its homologues promote bacterial adherence to human desquamated nasal epithelial cells.

Ultrastructural characterization of the nasal respiratory epithelium in the piglet.

http://dspace.nbuv.gov.ua/help/2015_Statistic.pdf