Establishment, Impacts, and Current Range of Spotted Knapweed (\u3ci\u3eCentaurea Stoebe\u3c/i\u3e Ssp. \u3ci\u3eMicranthos\u3c/i\u3e) Biological Control Insects in Michigan

Centaurea stoebe L. ssp. micranthos (Gugler) Hayek (spotted knapweed) is an invasive plant that has been the target of classical biological control in North America for more than four decades. Work in the western U.S. and Canada has shown the seedhead-feeding weevils Larinus minutus Gyllenhal and Larinus obtusus Gyllenhal (Coleoptera: Curculionidae) and the root-boring weevil Cyphocleonus achates (Fahraeus) (Coleoptera: Curculionidae) to be the most effective C. stoebe control agents. These three weevils have recently been introduced into the eastern U.S., including sites in Michigan in 2007 and 2009. In 2010, we made additional releases at six sites in Michigan, monitoring them for three years 2011-13. Here we report on the establishment, impact, and cur- rent range of L. minutus, L. obtusus, and C. achates in Michigan. We also report on the initial results of native plant overseeding treatments that were applied to biological control release sites with the aim of supplementing the nectar source C. stoebe provides. We found that L. minutus has established at all of its Michigan release sites and is widespread in the southwestern part of the state, while L. obtusus has established at the single site where it was released in 2007 and is spreading to adjoining counties. We also found C. achates to be present at four sites and established at one additional site in Michigan, but in all cases abundances are low and dispersal has been minimal (\u3c 10 m). In the three years following the 2010 releases, we found no measurable impacts of these biological control agents on C. stoebe growth, demographics, or plant community metrics. We also found little evidence of native flowering plant establishment at seeded sites. These baseline data will be useful in monitoring the spread and potential impacts of biological control agents on C. stoebe in Michigan

Inclusion Of The Spatial Dimension Of Population Data In Developing Policies For The Management Of AnGR –The Case Of The Heritage Sheep Breeds

The sustainable use of farm animal genetic resources is connected with the recognition of their contribution to the society and the environment and the assessment of the threats they are facing. The category of the heritage breeds, which are genetically distinct, geographically concentrated, adapted to their environment, commercially farmed to contribute to the local economy were considered in the frame of the HERITAGESHEEP project. The aim of this project was to deliver the potential of the heritage sheep breeds for a sustainable future for medium to low input production systems, which support local rural communities throughout Europe. This was achieved by addressing the conservation of these breeds, defining the current and future threats and developing new uses and markets for products

Automated CPX support system preliminary design phase

The development of the Distributed Command and Control System (DCCS) is discussed. The development of an automated C2 system stimulated the development of an automated command post exercise (CPX) support system to provide a more realistic stimulus to DCCS than could be achieved with the existing manual system. An automated CPX system to support corps-level exercise was designed. The effort comprised four tasks: (1) collecting and documenting user requirements; (2) developing a preliminary system design; (3) defining a program plan; and (4) evaluating the suitability of the TRASANA FOURCE computer model

Azidotetrakis(trimethylphosphine)nickel(II) Tetrafluoroborate

The title complex, [Ni(N3)(C3H9P)4]BF4, is a nearly perfect trigonal bipyramid with the azide group at an apical position. The metal-azide bond angle, Nil-- NlmN2, of 138.6(5) ° is the largest observed for a terminal azide ligand

The Rab-binding profiles of bacterial virulence factors during infection

Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the roles of most of the effectors are yet to be characterized. Key to understanding the function of effectors is the identification of host proteins they bind during infection. We previously developed a novel tandem-affinity purification (TAP) approach using hexahistidine and BirA-specific biotinylation tags for isolating translocated effector complexes from infected cells whose composition were subsequently deciphered by mass spectrometry. Here we further advanced the workflow for the TAP approach and determined the infection-dependent interactomes of the effectors SidM and LidA, which were previously reported to promiscuously bind multiple Rab GTPases in vitro. In this study we defined a stringent subset of Rab GTPases targeted by SidM and LidA during infection, comprising of Rab1A, 1B, 6, and 10; in addition, LidA targets Rab14 and 18. Taken together, this study illustrates the power of this approach to profile the intracellular interactomes of bacterial effectors during infection

The Rachel Carson Letters and the Making of Silent Spring

Environment, conservation, green, and kindred movements look back to Rachel Carson’s 1962 book Silent Spring as a milestone. The impact of the book, including on government, industry, and civil society, was immediate and substantial, and has been extensively described; however, the provenance of the book has been less thoroughly examined. Using Carson’s personal correspondence, this paper reveals that the primary source for Carson’s book was the extensive evidence and contacts compiled by two biodynamic farmers, Marjorie Spock and Mary T. Richards, of Long Island, New York. Their evidence was compiled for a suite of legal actions (1957-1960) against the U.S. Government and that contested the aerial spraying of dichlorodiphenyltrichloroethane (DDT). During Rudolf Steiner’s lifetime, Spock and Richards both studied at Steiner’s Goetheanum, the headquarters of Anthroposophy, located in Dornach, Switzerland. Spock and Richards were prominent U.S. anthroposophists, and established a biodynamic farm under the tutelage of the leading biodynamics exponent of the time, Dr. Ehrenfried Pfeiffer. When their property was under threat from a government program of DDT spraying, they brought their case, eventually lost it, in the process spent US$100,000, and compiled the evidence that they then shared with Carson, who used it, and their extensive contacts and the trial transcripts, as the primary input for Silent Spring. Carson attributed to Spock, Richards, and Pfeiffer, no credit whatsoever in her book. As a consequence, the organics movement has not received the recognition, that is its due, as the primary impulse for Silent Spring, and it is, itself, unaware of this provenance

Enantioconvergent Alkylations of Amines by Alkyl Electrophiles: Copper-Catalyzed Nucleophilic Substitutions of Racemic α-Halolactams by Indoles

Transition-metal catalysis has the potential to address shortcomings in the classic S_N2 reaction of an amine with an alkyl electrophile, both with respect to reactivity and to enantioselectivity. In this study, we describe the development of a user-friendly method (reaction at room temperature, with commercially available catalyst components) for the enantioconvergent nucleophilic substitution of racemic secondary alkyl halides (α-iodolactams) by indoles. Mechanistic studies are consistent with the formation of a copper(I)–indolyl complex that reacts at different rates with the two enantiomers of the electrophile, which interconvert under the reaction conditions (dynamic kinetic resolution). This investigation complements earlier work on photoinduced enantioconvergent N-alkylation, supporting the premise that this important challenge can be addressed by a range of strategies

Correlations, fluctuations and stability of a finite-size network of coupled oscillators

The incoherent state of the Kuramoto model of coupled oscillators exhibits marginal modes in mean field theory. We demonstrate that corrections due to finite size effects render these modes stable in the subcritical case, i.e. when the population is not synchronous. This demonstration is facilitated by the construction of a non-equilibrium statistical field theoretic formulation of a generic model of coupled oscillators. This theory is consistent with previous results. In the all-to-all case, the fluctuations in this theory are due completely to finite size corrections, which can be calculated in an expansion in 1/N, where N is the number of oscillators. The N -> infinity limit of this theory is what is traditionally called mean field theory for the Kuramoto model.Comment: 25 pages (2 column), 12 figures, modifications for resubmissio

Transcriptional Activation by NFκB Increases Perlecan/HSPG2 Expression in the Desmoplastic Prostate Tumor Microenvironment

Perlecan/HSPG2, a heparan sulfate proteoglycan typically found at tissue borders including those separating epithelia and connective tissue, increases near sites of invasion of primary prostatic tumors as previously shown for other proteins involved in desmoplastic tissue reaction. Studies of prostate cancer cells and stromal cells from both prostate and bone, the major site for prostate cancer metastasis, showed that cancer cells and a subset of stromal cells increased production of perlecan in response to cytokines present in the tumor microenvironment. In silico analysis of the HSPG2 promoter revealed two conserved NFκB binding sites, in addition to the previously reported SMAD3 binding sites. By systematically transfecting cells with a variety of reporter constructs including sequences up to 2.6 kb from the start site of transcription, we identified an active cis element in the distal region of the HSPG2 promoter, and showed that it functions in regulating transcription of HSPG2. Treatment with TNF-α and/or TGFβ1 identified TNF-α as a major cytokine regulator of perlecan production. TNF-α treatment also triggered p65 nuclear translocation and binding to the HSPG2 regulatory region in stromal cells and cancer cells. In addition to stromal induction of perlecan production in the prostate, we identified a matrix-secreting bone marrow stromal cell type that may represent the source for increases in perlecan in the metastatic bone marrow environment. These studies implicate perlecan in cytokine-mediated, innate tissue responses to cancer cell invasion, a process we suggest reflects a modified wound healing tissue response co-opted by prostate cancer cells