Novel colorimetric films based on starch/polyvinyl alcohol incorporated with roselle anthocyanins for fish freshness monitoring

Novel colorimetric films were developed for real-time monitoring of fish freshness based on starch/polyvinyl alcohol (SPVA) incorporated with roselle (Hibiseus sabdariffa L.) anthocyanins (RACNs). Firstly, RACNs were extracted from roselle dehydrated calyxes. Secondly, SPVA aqueous solution was obtained with a mass rate of 2:1 (starch/PVA). Thirdly, the colorimetric films were fabricated by immobilizing 30, 60 and 120 mg RACNs/100 g starch into SPVA matrix with casting/solvent evaporation method. FTIR spectra of the colorimetric films showed that RACNs were successfully immobilized into the SPVA matrix. X-ray diffraction spectra and SEM micrographs indicated that the crystallinity of PVA was reduced during the film-forming process and the compatibility between starch and PVA was improved, owing to the presence of RACNs. The incorporation of RACNs led to a decrease of water content and tensile strength and an increase of elongation at break of the colorimetric films compared with the SPVA film. The color stability test showed that the colorimetric films were stable at refrigeration temperature and room temperature up to 14 days with relative color changes below than 5%. The colorimetric films with lower content of RACNs were found more sensitive towards ammonia. An application trial was conducted to monitor the freshness of silver carp (Hypophthalmichthys molitrix) at refrigeration temperature. The colorimetric films presented visible color changes over time due to a variety of basic volatile amines known as total volatile basic nitrogen (TVB-N). Hence, these colorimetric films can be used to monitor the real-time fish freshness for intelligent packaging

3-d Lattice QCD Free Energy to Four Loops

We compute the expansion of the 3-d Lattice QCD free energy to four loop order by means of Numerical Stochastic Perturbation Theory. The first and second order are already known and are correctly reproduced. The third and fourth order coefficients are new results. The known logarithmic divergence in the fourth order is correctly identified. We comment on the relevance of our computation in the context of dimensionally reduced finite temperature QCD.Comment: 8 pages, 3 figures, latex typeset with JHEP3.cl

Drought events and their effects on vegetation productivity in China

Many parts of the world have experienced frequent and severe droughts during the last few decades. Most previous studies examined the effects of specific drought events on vegetation productivity. In this study, we characterized the drought events in China from 1982 to 2012 and assessed their effects on vegetation productivity inferred from satellite data. We first assessed the occurrence, spatial extent, frequency, and severity of drought using the Palmer Drought Severity Index (PDSI). We then examined the impacts of droughts on China\u27s terrestrial ecosystems using the Normalized Difference Vegetation Index (NDVI). During the period 1982–2012, China\u27s land area (%) experiencing drought showed an insignificant trend. However, the drought conditions had been more severe over most regions in northern parts of China since the end of the 1990s, indicating that droughts hit these regions more frequently due to the drier climate. The severe droughts substantially reduced annual and seasonal NDVI. The magnitude and direction of the detrended NDVI under drought stress varied with season and vegetation type. The inconsistency between the regional means of PDSI and detrended NDVI could be attributed to different responses of vegetation to drought and the timing, duration, severity, and lag effects of droughts. The negative effects of droughts on vegetation productivity were partly offset by the enhancement of plant growth resulting from factors such as lower cloudiness, warming climate, and human activities (e.g., afforestation, improved agricultural management practices)

Interferon-gamma Inducible Protein 10 up-regulated by acute-phase graft injury induced late-phase cisplatin resistance after liver transplantation

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Finite temperature Casimir pistons for electromagnetic field with mixed boundary conditions and its classical limit

In this paper, the finite temperature Casimir force acting on a two-dimensional Casimir piston due to electromagnetic field is computed. It was found that if mixed boundary conditions are assumed on the piston and its opposite wall, then the Casimir force always tends to restore the piston towards the equilibrium position, regardless of the boundary conditions assumed on the walls transverse to the piston. In contrary, if pure boundary conditions are assumed on the piston and the opposite wall, then the Casimir force always tend to pull the piston towards the closer wall and away from the equilibrium position. The nature of the force is not affected by temperature. However, in the high temperature regime, the magnitude of the Casimir force grows linearly with respect to temperature. This shows that the Casimir effect has a classical limit as has been observed in other literatures.Comment: 14 pages, 3 figures, accepted by Journal of Physics

Endophyte Chaetomium globosum D38 Promotes Bioactive Constituents Accumulation and Root Production in Salvia miltiorrhiza

Salvia miltiorrhiza is known for tanshinones and salvianolic acids, which have been shown to have a protective effect against ROS, especially for cardiovascular diseases and other various ailments of human organs. Due to the low yield of tanshinones and their analogs in S. miltiorrhiza, multiple stimulation strategies have been developed to improve tanshinones production in plant tissue cultures. Endophytic fungi have been reported to form different relationships with their host plants, including symbiotic, mutualistic, commensalistic, and parasitic interactions. Thus we take the assumption that endophytic fungi may be a potential microbial tool for secondary metabolism promotion in medicinal plants. We recently isolated Chaetomium globosum D38 from the roots of S. miltiorrhiza and our study aimed to examine the effects of this live endophytic fungus D38 and its elicitor on the accumulation of tanshinones in the hairy root cultures of S. miltiorrhiza. Our results revealed that C. globosum D38 mainly colonized in the intercellular gap of xylem parenchyma cells of S. miltiorrhiza hairy roots during the long term co-existence without any toxicity. Moreover, both of the live fungus and its mycelia extract could increase the production of tanshinones, especially for dihydrotanshinone I and cryptotanshinone. The effect of the mycelia extract was much stronger than that of the live fungus on tanshinones synthesis, which significantly increased the transcriptional activity of those key genes in tanshinone biosynthetic pathway. Furthermore, the live C. globosum D38 could also be made into biotic fertilizer used for S. miltiorrhiza seedlings culture, which not only significantly promoted the growth of the host plant, but also notably enhanced the accumulation of tanshinones and salvianolic acids. We thus speculated that, in the soil environment D38 could form bitrophic and mutual beneficial interactions with the host and enhance the plant growth and its secondary metabolism on the whole so as to have facilitative effects on both tanshinones and salvianolic acids accumulation. In conclusion, Chaetomium globosum D38 was a highly beneficial endophytic fungus for the growth and metabolism of S. miltiorrhiza

Optical character recognition on heterogeneous SoC for HD automatic number plate recognition system

Automatic number plate recognition (ANPR) systems are becoming vital for safety and security purposes. Typical ANPR systems are based on three stages: number plate localization (NPL), character segmentation (CS), and optical character recognition (OCR). Recently, high definition (HD) cameras have been used to improve their recognition rates. In this paper, four algorithms are proposed for the OCR stage of a real-time HD ANPR system. The proposed algorithms are based on feature extraction (vector crossing, zoning, combined zoning, and vector crossing) and template matching techniques. All proposed algorithms have been implemented using MATLAB as a proof of concept and the best one has been selected for hardware implementation using a heterogeneous system on chip (SoC) platform. The selected platform is the Xilinx Zynq-7000 All Programmable SoC, which consists of an ARM processor and programmable logic. Obtained hardware implementation results have shown that the proposed system can recognize one character in 0.63 ms, with an accuracy of 99.5% while utilizing around 6% of the programmable logic resources. In addition, the use of the heterogenous SoC consumes 36 W which is equivalent to saving around 80% of the energy consumed by the PC used in this work, whereas it is smaller in size by 95%