Glycosidases in pear pollen tube development

During the in vitro germination of pear pollen, several hydrolases were released into the medium. They were apparently eluted from the pollen grain, since the activity was the same when germination was inhibited. These enzymes, once released, had no role in tube growth, since resuspension of pollen in fresh medium after 1.5 hr of incubation did not result in a change of the subsequent tube growth. Homogenates of the pollen suspension at different stages of development showed no significant changes in phosphatase, β-glucosidase, or β-galactosidase activity. However, patent β-glucosidase activity measured directly in suspensions of intact pollen did increase after germination in proportion to tube wall development. Nojirimycin, a specific inhibitor of glucosidases, reduced this β-glucosidase activity by 75% at 10−5M and significantly reduced growth rate at 10−4

The Contribution of TP-AGB and RHeB Stars to the Near-IR Luminosity of Local Galaxies: Implications for Stellar Mass Measurements of High Redshift Galaxies

Using high spatial resolution HST WFC3 and ACS imaging of resolved stellar populations, we constrain the contribution of thermally-pulsing asymptotic giant branch (TP-AGB) stars and red helium burning (RHeB) stars to the 1.6 um near-infrared (NIR) luminosities of 23 nearby galaxies. The TP-AGB phase contributes as much as 17% of the integrated F160W flux, even when the red giant branch is well populated. The RHeB population contribution can match or even exceed the TP-AGB contribution, providing as much as 21% of the integrated F160W light. The NIR mass-to-light (M/L) ratio should therefore be expected to vary significantly due to fluctuations in the star formation rate over timescales from 25 Myr to several Gyr. We compare our observational results to predictions based on optically derived star formation histories and stellar population synthesis (SPS) models, including models based on the Padova isochrones (used in popular SPS programs). The SPS models generally reproduce the expected numbers of TP-AGB stars in the sample. The same SPS models, however, give a larger discrepancy in the F160W flux contribution from the TP-AGB stars, over-predicting the flux by a weighted mean factor of 2.3 +/-0.8. This larger offset is driven by the prediction of modest numbers of high luminosity TP-AGB stars at young (<300 Myrs) ages. The best-fit SPS models simultaneously tend to under-predict the numbers and fluxes of stars on the RHeB sequence, typically by a factor of 2.0+/-0.6 for galaxies with significant numbers of RHeBs. Coincidentally, over-prediction of the TP-AGB and under-prediction of the RHeBs result in a NIR M/L ratio largely unchanged for a rapid star formation rate. However, the NIR-to-optical flux ratio of galaxies could be significantly smaller than AGB-rich models would predict, an outcome that has been observed in some intermediate redshift post-starburst galaxies. (Abridged)Comment: 28 Pages, 12 Figures, 5 Tables, Accepted for Publication in the Astrophysical Journa

Resolved Near-infrared Stellar Populations in Nearby Galaxies

We present near-infrared (NIR) color-magnitude diagrams (CMDs) for the resolved stellar populations within 26 fields of 23 nearby galaxies (≲ 4 Mpc), based on images in the F110W and F160W filters taken with the Wide-Field Camera 3 (WFC3) on the Hubble Space Telescope (HST). The CMDs are measured in regions spanning a wide range of star formation histories, including both old dormant and young star-forming populations. We match key NIR CMD features with their counterparts in more familiar optical CMDs, and identify the red core helium-burning (RHeB) sequence as a significant contributor to the NIR flux in stellar populations younger than a few 100 Myr old. The strength of this feature suggests that the NIR mass-to-light ratio can vary significantly on short timescales in star-forming systems. The NIR luminosity of star-forming galaxies is therefore not necessarily proportional to the stellar mass. We note that these individual RHeB stars may also be misidentified as old stellar clusters in images of nearby galaxies. For older stellar populations, we discuss the CMD location of asymptotic giant branch (AGB) stars in the HST filter set and explore the separation of AGB subpopulations using a combination of optical and NIR colors. We empirically calibrate the magnitude of the NIR tip of the red giant branch in F160W as a function of color, allowing future observations in this widely adopted filter set to be used for distance measurements. We also analyze the properties of the NIR red giant branch (RGB) as a function of metallicity, showing a clear trend between NIR RGB color and metallicity. However, based on the current study, it appears unlikely that the slope of the NIR RGB can be used as an effective metallicity indicator in extragalactic systems with comparable data. Finally, we highlight issues with scattered light in the WFC3, which becomes significant for exposures taken close to a bright Earth limb

Social research on neglected diseases of poverty: Continuing and emerging themes

Copyright: © 2009 Manderson et al.Neglected tropical diseases (NTDs) exist and persist for social and economic reasons that enable the vectors and pathogens to take advantage of changes in the behavioral and physical environment. Persistent poverty at household, community, and national levels, and inequalities within and between sectors, contribute to the perpetuation and re-emergence of NTDs. Changes in production and habitat affect the physical environment, so that agricultural development, mining and forestry, rapid industrialization, and urbanization all result in changes in human uses of the environment, exposure to vectors, and vulnerability to infection. Concurrently, political instability and lack of resources limit the capacity of governments to manage environments, control disease transmission, and ensure an effective health system. Social, cultural, economic, and political factors interact and influence government capacity and individual willingness to reduce the risks of infection and transmission, and to recognize and treat disease. Understanding the dynamic interaction of diverse factors in varying contexts is a complex task, yet critical for successful health promotion, disease prevention, and disease control. Many of the research techniques and tools needed for this purpose are available in the applied social sciences. In this article we use this term broadly, and so include behavioral, population and economic social sciences, social and cultural epidemiology, and the multiple disciplines of public health, health services, and health policy and planning. These latter fields, informed by foundational social science theory and methods, include health promotion, health communication, and heath education

The Panchromatic Hubble Andromeda Treasury

The Panchromatic Hubble Andromeda Treasury (PHAT) is an on-going HST Multicycle Treasury program to image ~1/3 of M31's star forming disk in 6 filters, from the UV to the NIR. The full survey will resolve the galaxy into more than 100 million stars with projected radii from 0-20 kpc over a contiguous 0.5 square degree area in 828 orbits, producing imaging in the F275W and F336W filters with WFC3/UVIS, F475W and F814W with ACS/WFC, and F110W and F160W with WFC3/IR. The resulting wavelength coverage gives excellent constraints on stellar temperature, bolometric luminosity, and extinction for most spectral types. The photometry reaches SNR=4 at F275W=25.1, F336W=24.9, F475W=27.9, F814W=27.1, F110W=25.5, and F160W=24.6 for single pointings in the uncrowded outer disk; however, the optical and NIR data are crowding limited, and the deepest reliable magnitudes are up to 5 magnitudes brighter in the inner bulge. All pointings are dithered and produce Nyquist-sampled images in F475W, F814W, and F160W. We describe the observing strategy, photometry, astrometry, and data products, along with extensive tests of photometric stability, crowding errors, spatially-dependent photometric biases, and telescope pointing control. We report on initial fits to the structure of M31's disk, derived from the density of RGB stars, in a way that is independent of the assumed M/L and is robust to variations in dust extinction. These fits also show that the 10 kpc ring is not just a region of enhanced recent star formation, but is instead a dynamical structure containing a significant overdensity of stars with ages >1 Gyr. (Abridged)Comment: 48 pages including 22 pages of figures. Accepted to the Astrophysical Journal Supplements. Some figures slightly degraded to reduce submission siz

Discovery of a Gas-Rich Companion to the Extremely Metal-Poor Galaxy DDO 68

We present HI spectral-line imaging of the extremely metal-poor galaxy DDO 68. This system has a nebular oxygen abundance of only 3% Z$_{\odot}$, making it one of the most metal-deficient galaxies known in the local volume. Surprisingly, DDO 68 is a relatively massive and luminous galaxy for its metal content, making it a significant outlier in the mass-metallicity and luminosity-metallicity relationships. The origin of such a low oxygen abundance in DDO 68 presents a challenge for models of the chemical evolution of galaxies. One possible solution to this problem is the infall of pristine neutral gas, potentially initiated during a gravitational interaction. Using archival HI spectral-line imaging obtained with the Karl G. Jansky Very Large Array, we have discovered a previously unknown companion of DDO 68. This low-mass (M$_{\rm HI}$ $=$ 2.8$\times$10$^{7}$ M$_{\odot}$), recently star-forming (SFR$_{\rm FUV}$ $=$ 1.4$\times$10$^{-3}$ M$_{\odot}$ yr$^{-1}$, SFR$_{\rm H\alpha}$ $<$ 7$\times$10$^{-5}$ M$_{\odot}$ yr$^{-1}$) companion has the same systemic velocity as DDO 68 (V$_{\rm sys}$ $=$ 506 km s$^{-1}$; D $=$ 12.74$\pm$0.27 Mpc) and is located at a projected distance of 42 kpc. New HI maps obtained with the 100m Robert C. Byrd Green Bank Telescope provide evidence that DDO 68 and this companion are gravitationally interacting at the present time. Low surface brightness HI gas forms a bridge between these objects.Comment: Accepted for publication in the Astrophysical Journal Letter

Is there a metallicity ceiling to form carbon stars? - A novel technique reveals a scarcity of C stars in the inner M31 disk

We use medium-band near-infrared (NIR) Hubble Space Telescope WFC3 photometry with model NIR spectra of asymptotic giant branch (AGB) stars to develop a new tool for efficiently distinguishing carbon-rich (C-type) AGB stars from oxygen-rich (M-type) AGB stars in galaxies at the edge of and outside the Local Group. We present the results of a test of this method on a region of the inner disk of M31, where we find a surprising lack of C stars, contrary to the findings of previous C star searches in other regions of M31. We find only one candidate C star (plus up to six additional, less certain C star candidates), resulting in an extremely low ratio of C to M stars (C/M = (3.3^(+20)_(-0.1)) x 10^(-4)) that is one to two orders of magnitude lower than other C/M estimates in M31. The low C/M ratio is likely due to the high metallicity in this region which impedes stars from achieving C/O > 1 in their atmospheres. These observations provide stringent constraints to evolutionary models of metal-rich AGB stars and suggest that there is a metallicity threshold above which M stars are unable to make the transition to C stars, dramatically affecting AGB mass loss and dust production and, consequently, the observed global properties of metal-rich galaxies

The ALMA detection of CO rotational line emission in AGB stars in the Large Magellanic Cloud

Context. Low- and intermediate-mass stars lose most of their stellar mass at the end of their lives on the asymptotic giant branch (AGB). Determining gas and dust mass-loss rates (MLRs) is important in quantifying the contribution of evolved stars to the enrichment of the interstellar medium. Aims: This study attempts to spectrally resolve CO thermal line emission in a small sample of AGB stars in the Large Magellanic Cloud (LMC). Methods: The Atacama Large Millimeter Array was used to observe two OH/IR stars and four carbon stars in the LMC in the CO J = 2-1 line. Results: We present the first measurement of expansion velocities in extragalactic carbon stars. All four C stars are detected and wind expansion velocities and stellar velocities are directly measured. Mass-loss rates are derived from modelling the spectral energy distribution and Spitzer/IRS spectrum with the DUSTY code. The derived gas-to-dust ratios allow the predicted velocities to agree with the observed gas-to-dust ratios. The expansion velocities and MLRs are compared to a Galactic sample of well-studied relatively low MLRs stars supplemented with extreme C stars with properties that are more similar to the LMC targets. Gas MLRs derived from a simple formula are significantly smaller than those derived from dust modelling, indicating an order of magnitude underestimate of the estimated CO abundance, time-variable mass loss, or that the CO intensities in LMC stars are lower than predicted by the formula derived for Galactic objects. This could be related to a stronger interstellar radiation field in the LMC. Conclusions: Although the LMC sample is small and the comparison to Galactic stars is non-trivial because of uncertainties in their distances (hence luminosities), it appears that for C stars the wind expansion velocities in the LMC are lower than in the solar neighbourhood, while the MLRs appear to be similar. This is in agreement with dynamical dust-driven wind models