Cessation of Nightly Voluntary Wheel Running Activity Following Exposure to a Mouse Model of Posttraumatic Stress

Regular physical activity (PA) is well known to positively impact physical and mental health outcomes. In our work to examine cardiovascular benefits of PA in a mouse model of posttraumatic stress, we stumbled upon the reciprocal relationship between PA and stress exposure, wherein stress significantly reduced healthy levels of routine PA. The aim of the present studies was to define the parameters of our paradigm. C67BL/6J male mice were divided into four groups (n=8/group): sedentary/control, voluntary running/control, sedentary/stress, and voluntary running/stress. Voluntary running groups were given unlimited access to a running wheel for 9 weeks. Stress groups were then exposed to a 5-day resident-intruder social stress that models human posttraumatic stress. Running behavior essentially ceased following stress. Habituation to stress occurred, as running distance increased by the 5th day of stress but remained significantly low. A separate study examined a single exposure to resident-intruder social stress. Plasma corticosterone significantly increased while nightly running dropped significantly but returned to normal by the 3rd night post-stress. These studies show the sensitivity of habitual running behavior to stress exposure and suggest the utility of this mouse model in exploring the means by which stress negatively impacts routine PA

Quantification of transport across the boundary of the lower stratospheric vortex during Arctic winter 2002/2003

Strong perturbations of the Arctic stratosphere during the winter 2002/2003 by planetary waves led to enhanced stretching and folding of the vortex. On two occasions the vortex in the lower stratosphere split into two secondary vortices that re-merged after some days. As a result of these strong disturbances the role of transport in and out of the vortex was stronger than usual. An advection and mixing simulation with the Chemical Lagrangian Model of the Stratosphere (CLaMS) utilising a suite of inert tracers tagging the original position of the air masses has been carried out. The results show a variety of synoptic and small scale features in the vicinity of the vortex boundary, especially long filaments peeling off the vortex edge and being slowly mixed into the mid latitude environment. The vortex folding events, followed by re-merging of different parts of the vortex led to strong filamentation of the vortex interior. During January, February, and March 2003 flights of the Russian high-altitude aircraft Geophysica were performed in order to probe the vortex, filaments and in one case the merging zone between the secondary vortices. Comparisons between CLaMS results and observations obtained from the Geophysica flights show in general good agreement. Several areas affected by both transport and strong mixing could be identified, allowing explanation of many of the structures observed during the flights. Furthermore, the CLaMS simulations allow for a quantification of the air mass exchange between mid latitudes and the vortex interior. The simulation suggests that after the formation of the vortex was completed, its interior remaind relatively undisturbed. Only during the two re-merging events were substantial amounts of extra-vortex air transported into the polar vortex. When in March the vortex starts weakening additional influence from lower latitudes becomes apparent in the model results. In the lower stratosphere export of vortex air leads only to a fraction of about 5% polar air in mid latitudes by the end of March. An upper limit for the contribution of ozone depleted vortex air on mid-latitude ozone loss is derived, indicating that the maximum final impact of dilution is on the order of 50%

Drag reduction induced by superhydrophobic surfaces in turbulent pipe flow

The drag reduction induced by superhydrophobic surfaces is investigated in a turbulent pipe flow. Wetted superhydrophobic surfaces are shown to trap gas bubbles in their asperities. This stops the liquid from coming in direct contact with the wall in that location, allowing the flow to slip over the air bubbles. We consider a well-defined texture with streamwise grooves at the walls in which the gas is expected to be entrapped. This configuration is modeled with alternating no-slip and shear-free boundary conditions at the wall. With respect to the classical turbulent pipe flow, a substantial drag reduction is observed which strongly depends on the grooves’ dimension and on the solid fraction, i.e., the ratio between the solid wall surface and the total surface of the pipe’s circumference. The drag reduction is due to the mean slip velocity at the wall which increases the flow rate at a fixed pressure drop. The enforced boundary conditions also produce peculiar turbulent structures which on the contrary decrease the flow rate. The two concurrent effects provide an overall flow rate increase as demonstrated by means of the mean axial momentum balance. This equation provides the balance between the mean pressure gradient, the Reynolds stress, the mean flow rate, and the mean slip velocity contribution

Dislocation core field. I. Modeling in anisotropic linear elasticity theory

Aside from the Volterra field, dislocations create a core field, which can be modeled in linear anisotropic elasticity theory with force and dislocation dipoles. We derive an expression of the elastic energy of a dislocation taking full account of its core field and show that no cross term exists between the Volterra and the core fields. We also obtain the contribution of the core field to the dislocation interaction energy with an external stress, thus showing that dislocation can interact with a pressure. The additional force that derives from this core field contribution is proportional to the gradient of the applied stress. Such a supplementary force on dislocations may be important in high stress gradient regions, such as close to a crack tip or in a dislocation pile-up

Amyloid-based nanosensors and nanodevices

This journal is © The Royal Society of Chemistry 2014Self-assembling amyloid-like peptides and proteins give rise to promising biomaterials with potential applications in many fields. Amyloid structures are formed by the process of molecular recognition and self-assembly, wherein a peptide or protein monomer spontaneously self-associates into dimers and oligomers and subsequently into supramolecular aggregates, finally resulting in condensed fibrils. Mature amyloid fibrils possess a quasi-crystalline structure featuring a characteristic fiber diffraction pattern and have well-defined properties, in contrast to many amorphous protein aggregates that arise when proteins misfold. Core sequences of four to seven amino acids have been identified within natural amyloid proteins. They are capable to form amyloid fibers and fibrils and have been used as amyloid model structures, simplifying the investigations on amyloid structures due to their small size. Recent studies have highlighted the use of self-assembled amyloid-based fibers as nanomaterials. Here, we discuss the latest advances and the major challenges in developing amyloids for future applications in nanotechnology and nanomedicine, with the focus on development of sensors to study protein–ligand interactions.This work is supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore). Dr Ivo C. Martins acknowledges the funding from the Portuguese Foundation for Science and Technology (project PTDC/SAU-ENB/117013/2010 and program Investigador FCT, Research Contract IF/00772/2013)

Channel flow with large longitudinal ribs

We present data from direct numerical simulations of flow through channels containing large, longitudinal, surface-mounted, rectangular ribs at various spanwise spacings, which lead to secondary flows. It is shown that appropriate modifications to the classical log-law, predicated on a greater wetted surface area than in a plane channel, lead to a log-law-like region in the spanwise-averaged axial mean velocity profiles, even though local profiles may be very different. The secondary flows resulting from the presence of the ribs are examined and their effects discussed. Comparing our results with the literature we conclude that the sense of the secondary flows is largely independent of the particular rib spacing whether normalised by channel depth or rib width. The strength of the secondary flows, however, is shown to depend on the ratio of rib spacing to rib width and on Reynolds number. Topological features of the secondary flow structure are illustrated via a critical point analysis and shown to be characterised in all cases by a free stagnation point above the centre of the rib. Finally, we show that if the domain size is chosen as a ‘minimal channel’ size, rather than a size which allows adequate development of the usual outer layer flow structures, the secondary flows can be affected and this leads inevitably to differences in the near-rib flows so that for ribbed channels, unlike plain channels, it is unwise to use minimal domains to identify details of the near-wall flow

Translation of Anticancer Efficacy From Nonclinical Models to the Clinic

Mouse cancer models have provided critical insights into tumor biology; however, clinical translation of these findings has been challenging. This perspective posits that factors impacting on successful translation start with limitations in capturing human cancer pathophysiology and end with challenges in generating robust translatable preclinical end points. A comprehensive approach that considers clinically relevant mouse models with both an integrated biomarker strategy and a complementary modeling and simulation effort will strengthen the current oncology drug development paradigm