The use and effectiveness of behavioural modification techniques in achieving and maintaining normal weight and fitness – the lifestyle changes for adults in Cyprus

BACKGROUND and AIM: Research showed that behavioural modification techniques were the most effective way to achieve and maintain a healthy weight compared to diet and exercise alone. If behavioural modification was the most effective technique, then at least the two thirds of the candidates (Weight Loss Candidate with Behaviour Modification-WLCB) who used the specific behavioural modification techniques regarding eating and physical activity would achieve a weight loss of average ½ -1kg per week for the 18 weeks of the treatment and then maintain it or continue to lose after treatment termination with a total treatment period of 36 weeks. The treatment was efficient and long-lasting compared to the control candidates who followed a diet plan only. Furthermore, if the use of behavioural modification was the most effective way for diet and exercise, then the weight loss and maintenance would be more efficient and long-lasting. The principal aim of this project was to develop behaviour modification techniques (protocol and guidelines) in order to promote effective weight management for sedentary and active Cypriot adults, concentrating on nutrition and exercise. STUDY DESIGN: The study was quantitative and qualitative in nature using an action research approach. Furthermore, it was observational and longitudinal using structured methods and a representative sample of adults 19-50 years of age is investigated for 36 weeks. METHODS: Anthropometric data and health and diet history were taken using standard methods from 315 (the control and intervention group (1:1) Cypriot adults from rural and urban areas. Individualised diet plans, personalised physical activities as well as behavioural modification parameters were assessed with the aid of a questionnaire, interview, food and exercise diary and behavioural checklist. Obesity and overweight were defined using the current International Obesity Task Force definition. The validated questionnaires for Physical Activity (IPAQ) and the nutritional assessment (National Integrated Health Associates) were used for initial assessment and the behavioural checklists were used for the follow up. Behavioural checklists were completed by the candidates on a bi-weekly basis along with the food and exercise diary. The questionnaire reliability (test-retest), internal consistency and ability to differentiate obese vs. normal-weight adult behaviours were tested using a pilot population of 50 adults not enrolled in a formal weight loss programme. Its reliability (checklists, food and exercise diary) and predictive validity were tested using a selective population: 265 adults of the observational group enrolled in behavioural modification treatment vs. diet. OBJECTIVES OF THE STUDY: Τhe objectives of the research were a) The study of every aspect of weight management and thus be capable of making suggestions for the development of guidelines and protocols for behavioural modification to health professionals working with the public. b) The identification and adjustment of eating and exercise behaviour related to body weight in order to achieve an efficient weight management in Cypriot overweight/obese adults. c) The evaluation of the effectiveness of the modest lifestyle changes in weight management induced by short-term energy/calorie restriction and behaviour modification in overweight and obese Greek Cypriots with Body Mass Index (BMI) above 25 or 30 respectively, or body weight 20% or above of Ideal Body weight (IBW), or body fat above normal levels. d) The development of education and counselling protocols and guidelines for professionals for weight management protocols based on the Mediterranean Diet and the eating and exercise habits of Cypriot Adults. e) The implementation of the protocols to intervention groups in order to identify the efficiency. OUTCOMES: The outcomes of the project was the development of an education and counselling protocol/manual which included teaching tools and guidelines for professionals for weight management with the title “Protocol and Guidelines for professional users for weight management for Adults: assessment, categorization, therapy/ follow up of overweight and obesity”. The development of an eating behaviour and physical activity protocol/manual based on the Mediterranean Diet and the demonstrated eating and exercise habits of Cypriot Adults can be used by the stakeholders and the public for efficient and effective weight management. RESULTS: The average length of weight loss treatment was 18 weeks for the intervention and the control groups, and the average weight loss for the intervention group was 11.96kg (t-test, p-value ≤ 0.001) and for the control group was 5.56kg (t-test, p-value≤ 0.002). During the 18 weeks of the maintenance period the 50% of the intervention group continued to lose while, an additional 48% of them maintained the weight and only 2% of them gained weight. CONCLUSIONS: To conclude, the prevalence of obesity worldwide has reached epidemic proportions. Physical inactivity and poor diet have been identified as primary contributors to the leading causes of death in developed countries including Cyprus. It is unfortunate that more emphasis is given to calorie restriction dieting rather than behavioural modification for diet and physical activity in achieving and maintaining weight loss. The fact is that incorporating appropriate behavioural modification techniques for diet and sufficient physical activity into one’s life is an essential component of achieving a healthy body weight. The research project aimed at developing a behavioural approach and behavioural modification techniques for the treatment of obesity and weight management adjusted to the needs of the Cypriot adult population. The difference of this approach underlines the emphasis that has to be given to the follow-up programme for the maintenance of the weight loss through behavioural modification. This approach evolved from the environmental control of eating behaviour to a broader approach characterized by systematic manipulation of all factors associated with eating and exercise patterns. The need for the project derives from the fact that obesity is a chronic condition with a substantial potential for relapse; therefore long–term treatments are needed. The expected outcomes of this project are the development of guidelines and protocols to be used by health professionals. The ultimate goal is the reduction of the obesity epidemic in Cyprus

Generalized Heisenberg algebras and k-generalized Fibonacci numbers

It is shown how some of the recent results of de Souza et al. [1] can be generalized to describe Hamiltonians whose eigenvalues are given as k-generalized Fibonacci numbers. Here k is an arbitrary integer and the cases considered by de Souza et al. corespond to k=2.Comment: 8 page

Probabilistic Mobility Models for Mobile and Wireless Networks

International audienceIn this paper we present a probabilistic broadcast calculus for mobile and wireless networks whose connections are unreliable. In our calculus, broadcasted messages can be lost with a certain probability, and due to mobility the connection probabilities may change. If a network broadcasts a message from a location, it will evolve to a network distribution depending on whether nodes at other locations receive the message or not. Mobility of nodes is not arbitrary but guarded by a probabilistic mobility function (PMF), and we also define the notion of a weak bisimulation given a PMF. It is possible to have weak bisimular networks which have different probabilistic connectivity information. We furthermore examine the relation between our weak bisimulation and a minor variant of PCTL* [1]. Finally, we apply our calculus on a small example called the Zeroconf protocol [2]

Roles of fibrin α- and γ-chain specific cross-linking by FXIIIa in fibrin structure and function

Factor XIII is responsible for the cross-linking of fibrin γ-chains in the early stages of clot formation, whilst α-chain cross-linking occurs at a slower rate. Although γ- and α-chain cross-linking was previously shown to contribute to clot stiffness, the role of cross-linking of both chains in determining clot structure is currently unknown. Therefore, the aim of this study was to determine the role of individual α- and γ-chain cross-linking during clot formation, and its effects on clot structure. We made use of a recombinant fibrinogen (γQ398N/Q399N/K406R), which does not allow for γ-chain cross-linking. In the absence of cross-linking, intact D-D interface was shown to play a potential role in fibre appearance time, clot stiffness and elasticity. Cross-linking of the fibrin α-chain played a role in the thickening of the fibrin fibres over time, and decreased lysis rate in the absence of α2-antiplasmin. We also showed that α-chain cross-linking played a role in the timing of fibre appearance, straightening fibres, increasing clot stiffness and reducing clot deformation. Cross-linking of the γ-chain played a role in fibrin fibre appearance time and fibre density. Our results show that α- and γ-chain cross-linking play independent and specific roles in fibrin clot formation and structure

ARIADNE - A novel optical LArTPC: technical design report and initial characterisation using a secondary beam from the CERN PS and cosmic muons

ARIADNE is a 1-ton (330 kg fiducial mass) dual-phase liquid argon (LAr) time projection chamber (TPC) featuring a novel optical readout. Four electron-multiplying charge-coupled device (EMCCD) cameras are mounted externally, and these capture the secondary scintillation light produced in the holes of a thick electron gas multiplier (THGEM). Track reconstruction using this novel readout approach is demonstrated. Optical readout has the potential to be a cost effective alternative to charge readout in future LArTPCs. In this paper, the technical design of the detector is detailed. Results of mixed particle detection using a secondary beam from the CERN PS (representing the first ever optical images of argon interactions in a dual-phase LArTPC at a beamline) and cosmic muon detection at the University of Liverpool are also presented.Comment: 58 pages, 40 figures. Changes from previous version based on pre-publication review: improved quality of various figures, improved clarity of some definitions and reduced longer sentences for better readability, fixed typos and formatting error

The interaction between fibrinogen and zymogen FXIII-A₂B₂ is mediated by fibrinogen residues γ390-396 and the FXIII-B subunits

Coagulation transglutaminase factor XIII (FXIII) exists in circulation as heterotetrameric proenzyme FXIII-A2B2. Effectively all FXIII-A2B2 circulates bound to fibrinogen, and excess FXIII-B2 circulates in plasma. The motifs that mediate interaction of FXIII-A2B2 with fibrinogen have been elusive. We recently detected reduced binding of FXIII-A2B2 to murine fibrinogen that has γ-chain residues 390-396 mutated to alanines (Fibγ390-396A). Here, we evaluated binding features using human components, including recombinant fibrinogen variants, FXIII-A2B2, and isolated FXIII-A2 and -B2 homodimers. FXIII-A2B2 coprecipitated with wild-type (γA/γA), alternatively-spliced (γ′/γ′), and αC-truncated (Aα251) fibrinogens, whereas coprecipitation with human Fibγ390-396A was reduced by 75% (P < .0001). Surface plasmon resonance showed γA/γA, γ′/γ′, and Aα251 fibrinogens bound FXIII-A2B2 with high affinity (nanomolar); however, Fibγ390-396A did not bind FXIII-A2B2. These data indicate fibrinogen residues γ390-396 comprise the major binding motif for FXIII-A2B2. Compared with γA/γA clots, FXIII-A2B2 activation peptide release was 2.7-fold slower in Fibγ390-396A clots (P < .02). Conversely, activation of recombinant FXIII-A2 (lacking FXIII-B2) was similar in γA/γA and Fibγ390-396A clots, suggesting fibrinogen residues γ390-396 accelerate FXIII-A2B2 activation in a FXIII-B2–dependent mechanism. Recombinant FXIII-B2 bound γA/γA, γ′/γ′, and Aα251 with similar affinities as FXIII-A2B2, but did not bind or coprecipitate with Fibγ390-396A. FXIII-B2 also coprecipitated with fibrinogen from FXIII-A–deficient mouse and human plasmas. Collectively, these data indicate that FXIII-A2B2 binds fibrinogen residues γ390-396 via the B subunits, and that excess plasma FXIII-B2 is not free, but rather circulates bound to fibrinogen. These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations

Reversibility in Chemical Reactions

open access bookIn this chapter we give an overview of techniques for the modelling and reasoning about reversibility of systems, including outof- causal-order reversibility, as it appears in chemical reactions. We consider the autoprotolysis of water reaction, and model it with the Calculus of Covalent Bonding, the Bonding Calculus, and Reversing Petri Nets. This exercise demonstrates that the formalisms, developed for expressing advanced forms of reversibility, are able to model autoprotolysis of water very accurately. Characteristics and expressiveness of the three formalisms are discussed and illustrated

Large-scale analysis of Drosophila core promoter function using synthetic promoters

The core promoter plays a central role in setting metazoan gene expression levels, but how exactly it “computes” expression remains poorly understood. To dissect its function, we carried out a comprehensive structure–function analysis in Drosophila. First, we performed a genome-wide bioinformatic analysis, providing an improved picture of the sequence motifs architecture. We then measured synthetic promoters’ activities of ~3,000 mutational variants with and without an external stimulus (hormonal activation), at large scale and with high accuracy using robotics and a dual luciferase reporter assay. We observed a strong impact on activity of the different types of mutations, including knockout of individual sequence motifs and motif combinations, variations of motif strength, nucleosome positioning, and flanking sequences. A linear combination of the individual motif features largely accounts for the combinatorial effects on core promoter activity. These findings shed new light on the quantitative assessment of gene expression in metazoans

Submesothelial deposition of carbon nanoparticles after toner exposition: Case report

Inhalation of carbon nanoparticles (CNP) from toner dust has been shown to have impact on the respiratory health of persons exposed. Office printers are known emitters of CNP. We report about a female open office worker who developed weight loss and diarrhoea. Laparoscopy done for suspected endometriosis surprisingly revealed black spots within the peritoneum. Submesothelial aggregates of CNP with a diameter of 31-67 nm were found by scanning and transmission electron microscopy in these tissue specimens. Colon biopsies showed inflammatory bowel disease with typically signs of Crohn disease, but no dust deposits. Transport of CNP via lymphatic and blood vessels after inhalation in the lungs has to be assumed. In this case respiratory symptoms were not reported, therefore no lung function tests were done. We have shown that workers with toner dust exposure from laser printers can develop submesothelial deposition of CNP in the peritoneum. Impact of toner dust exposure on the respiratory health of office workers, as suspected in other studies, has to be evaluated further