3,104 research outputs found
Erupted Complex Odontoma Mimicking a Mandibular Second Molar
Complex odontoma (CO) is considered one of the most common odontogenic lesions, composed by a miscellaneous of dental tissue such as enamel, dentin, pulp and sometimes cementum. They may interfere with the eruption of an associated tooth, being more prevalent in the posterior mandible. CO has been rarely reported as erupted, being considered an intraosseous lesion. This is a case report of a 17-year-old male with a benign fibro-osseous lesion consistent with CO that was located at the left second molar region, above the crown of the impacted mandibular second molar tooth. The lesion was surgically removed, and the tooth had to be extracted, since there was no indication that it could erupt naturally or with orthodontic traction. The histopathological examination confirmed the diagnosis of CO and after 6 months complete bone formation was observed radiographically. An early diagnosis will provide a better treatment option, avoiding tooth extraction or a more damaging surgery
Histologic and Histomorphometric Analysis of Posterior Region of the Human Temporomandibular Disc
Objective The aim of this study was to analyze histologic and histomorphometric features of the articular disc in groups with and without disc displacement. Study design A sample of 39 temporomandibular joints TMJs (31 case specimens, 8 control specimens) from 28 patients (mean age 31.2 years) were recruited for this study. The patients were considered to be affected and treated surgically with disc repositioning when presenting painful clinical signs of disc displacement after unsuccessful nonsurgical treatment for at least 6 months. Of the control patients, 4 presented condyle fracture which required opening to be reduced for treatment, and 4 displayed active condyle hyperplasia. The posterior region of the disc was removed and sent for histologic and histomorphometric analysis. Histologic (hematoxylin-eosin) and histomorphometric (picro-Sirius red) analyses were performed. Statistically significant differences between the analyzed groups were accessed through the chi-squared test (P ≤ .05). The Mann-Whitney U test was used to observe the differences between mean values when variables did not present normal distribution [Kolmogorov-Smirnov(a) test]. Results There were no significant differences between the groups in relation to the parameters studied by histologic and histomorphometric analysis (using or not using polarization). Conclusions To the limits of this study, there were no significant histologic and histomorphometric differences in the articular disc between groups with and without TMJ dysfunction
FasL Expression in Articular Discs of Human Temporomandibular Joint and Association with Osteoarthrosis
Background
Apoptosis is a programme of cell death which does not induce an inflammatory response. Recent previous research has suggested a correlation between temporomandibular internal derangement and apoptosis. Fas ligand (FasL) is an apoptosis‐inducing factor, known to trigger apoptosis through distinct signal pathways. This study aims to examine, by immunohistochemistry, the expression of FasL in temporomandibular joint (TMJ) articular discs of patients with anterior disc displacement with reduction (ADDwR) and without reduction (ADDwoR) in patients with and without osteoarthrosis (OA). Methods
Forty‐two (n = 42) TMJ articular discs were divided into two cut‐offs: (i) 8 control, 17 ADDwR, 17 ADDwoR, and (ii) without OA (n = 25) and with OA (n = 17). The area of immunostaining was compared statistically between groups (P \u3c 0.05). Results
Statistically significant differences were found in the expression of FasL in TMJ discs between the three groups (P = 0.001). ADDwR presented significant higher FasL expression when compared with ADDwoR (P \u3c 0.001). Significant higher FasL expression was observed in the group without OA (P = 0.001). All patients without OA presented ADDwR, while all the patients with OA presented ADDwoR. Conclusion
A higher area of in situ immunostaining of FasL was found in temporomandibular discs with reduction, which is the less severe condition. Moreover, a reduced expression of FasL in the discs of patients with osteoarthrosis was found, suggesting that some aspects of apoptosis might underlie the progression of TMJ disorders
Biodiversidade, população e economia: uma região de mata atlântica [Biodiversity, Population, and Economy: a region of atlantic forest]
Minas Gerais; Rio Doce; mata atlântica; atlantic forest; sustainable development; conservation; regional development; environment
A Leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of Leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasis
Background: LiHyV is an antigenic hypothetical protein present in both promastigote and amastigote stages of Leishmania infantum, which was recently identified by an immunoproteomic approach. A recombinant version of this protein (rLiHyV) was evaluated as a diagnostic marker for canine VL (CVL). In addition, the prophylactic efficacy of the rLiHyV protein, and two of its CD8+ T cell epitopes, has been analyzed in a murine model of visceral leishmaniasis (VL).
Methods: Initially, the rLiHyV protein was evaluated by an ELISA technique for the serodiagnosis of CVL. Secondly, vaccines composed of the recombinant protein and both chemically synthesized peptides, combined with saponin as an adjuvant; were administered subcutaneously into BALB/c mice. The cellular and humoral responses generated by vaccination were evaluated. In addition, the parasite burden and immune response were studied 10 weeks after L. infantum infection.
Results: The rLiHyV protein was recognized by antibodies of VL dogs. No cross-reactivity was obtained with sera from dogs vaccinated with a Brazilian commercial vaccine, with sera from animals infected with Trypanosoma cruzi, Babesia canis and Ehrlichia canis, or those from non-infected animals living in an endemic area for leishmaniasis. After challenge with L. infantum, spleen cells of BALB/c mice vaccinated with rLiHyV/saponin stimulated with parasite antigens showed
a higher production of IFN-γ, IL-12 and GM-CSF, than the same cells obtained from mice vaccinated with the individual peptides, or mice from control (inoculated with saline or saponin) groups. This Th1-type cellular response observed in rLiHyV/saponin vaccinated mice was accompanied by the induction of parasite-specific IgG2a isotype antibodies. Animals immunized with rLiHyV/saponin showed significant reductions in the parasite burden in the liver, spleen, bone marrow and in the lymph nodes draining the paws relative to control mice.
Conclusions: The present study showed for the first time that the L. infantum LiHyV protein could be considered as a vaccine candidate against L. infantum infection, as well as a diagnostic marker for CVL.This work was supported by grants from Instituto Nacional de Ciência e Tecnologia em Nanobiofarmacêutica (INCT-Nanobiofar), FAPEMIG (CBB-APQ-00819-12), and CNPq (APQ-472090/2011-9, RHAE-456287/2012-4, APQ-482976/2012-8, and APQ-488237/2013-0). MACF is a grant recipient of FAPEMIG/CAPES. EAFC and APF are grant recipient of CNPq.Peer Reviewe
WRONG WAY HOME: AN INFANT SOUTHERN ELEPHANT SEAL (MIROUNGA LEONINA) ARRIVAL ON SOUTHEASTERN BRAZILIAN BEACHES
ABSTRACTRecords of southern elephant seals (Mirounga leonina) as vagrants along the SE Brazilian coast date back the late 50’s and have been relatively common over the last decades. These large marine mammals usually call much attention when they arrive on tropical beaches worldwide and are generally treated as ‘occasional visitors’ and ‘vagrants’. This note reports on sightings of a youngelephant seal along SE Brazil in the summer and autumn of 2020. We also reviewed records in both the literature and open sources, totaling eight records of infant southern elephant seals known since the late 70’s along the Brazilian coast. It was noted that the arrival of an infant in February of 2020is coincident with a previous cyclonic activity off the SE and NE Brazilian coast. The connection of such unlikely records of pinnipeds on tropical beaches and extreme weather events associated to ocean currents should be better evaluated in the context of climatic change RESUMOÉ reportada uma ocorrência recente de um infante de elefante-marinho (Mirounga leonina) na costa do estado do Rio de Janeiro com um intervalo aproximado de um mês entre as avistagens do mesmo indivíduo. A comparação das fotografias obtidas em ambos os registros permitiu a comparação e confirmação. Em adição, se discute a presença de infantes na costa brasileira, que totalizam oito casos, mas que não apontam uma sazonalidade marcada, mas uma tendência aos registros serem reportados em junho, seguido por outubro e novembro. Como o presente registro se deu em janeiro, pico do verão, fatores climáticos de larga escala podem estar atuando para deslocar alguns indivíduos muito ao longe das suas colônias.Palavras-chave: Mirounga leonina; Movimentos; Vagante; Atlântico Sul
Desenvolvimento, caracterização e validação clínica de um novo ensaio sensível para a dosagem da tiroglobulina sérica
OBJECTIVE: In the last decade, data published stressed the role of highly-sensitive thyroglobulin (Tg) assays in the follow-up of differentiated thyroid carcinoma (DTC) patients. The present study describes a new, highly-sensitive Tg assay, compares it with an available commercial assay, and validates it in the follow-up of DTC patients. SUBJECTS AND METHODS: The immunofluorometric high-sensitivity Tg assay is based on monoclonal and polyclonal antibodies produced at our laboratories. It was validated in 100 samples of 87 patients with DTC submitted to total thyroidectomy, 87% of whom also received radioiodine. For correlation, all samples were also tested using a commercial Tg assay (Beckman Access) with functional sensitivity (FS) of 0.1 ng/mL. RESULTS: The new method showed FS of 0.3 ng/mL. The correlation between the two methods was good (r = 0.74; p < 0.0001). The diagnostic sensitivity was 88.9%, and it was increased to 100% when combined with neck US. CONCLUSION: This new, high-sensitivity Tg assay presented a good correlation with Beckman Access assay and with the clinical outcome of the patients. The continuous availability of a validated assay is an additional advantage for long term follow-up of DTC patients. Arq Bras Endocrinol Metab. 2012;56(9):658-65OBJETIVO: Na última década, estudos mostraram a importância dos ensaios de tiroglobulina (Tg) com melhor sensibilidade funcional no seguimento dos pacientes com carcinoma diferenciado de tiroide (CDT). Neste estudo, descrevemos o desenvolvimento de um novo ensaio de Tg de alta sensibilidade, que foi validado no seguimento de pacientes com CDT e correlacionado com um ensaio comercialmente disponível. SUJEITOS E MÉTODOS: O ensaio imunofluorométrico de Tg baseia-se em anticorpos, um monoclonal e um policlonal desenvolvidos em nosso laboratório. Avaliamos 100 amostras de soro de 87 pacientes com CDT submetidos à tiroidectomia total, sendo que 87% deles também receberam 131I. A Tg foi dosada também em ensaio comercial (Beckman Access). RESULTADOS: A correlação entre os dois métodos foi de 0,74 (p < 0,0001). O novo ensaio mostrou uma sensibilidade funcional de 0,3 ng/mL. A sensibilidade diagnóstica foi de 88,9%, que aumentou para 100% quando associada ao ultrassom cervical (US). CONCLUSÃO: O novo método de dosagem de Tg mostra boa correlação com o ensaio comercial Beckman Access e com a evolução clínica dos pacientes. O novo ensaio será fundamental no seguimento dos nossos pacientes com CDT. Arq Bras Endocrinol Metab. 2012;56(9):658-65Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaUNIFESP, EPM, Depto. de MedicinaSciEL
DECOMPOSITION OF LEGUME TREE Gliricidia sepium AND Acacia angustissima IN AGROFORESTRY SYSTEMS
Os Sistemas Agroflorestais (SAFs) trazem benef\uedcios m\ufaltiplos
e constituem uma alternativa para minimizar a degrada\ue7\ue3o
ambiental e alcan\ue7ar um desenvolvimento sustent\ue1vel, devido
\ue0 diversidade de esp\ue9cies que os comp\uf5e. O presente
estudo avaliou a contribui\ue7\ue3o das leguminosas arb\uf3reas,
Gliricidia sepium e Acacia angustissima , cultivadas em aleias
intercaladas com banana (Musa sp.) e a\ue7a\ued ( Euterpe oleraceae
), na implanta\ue7\ue3o de Sistema Agroflorestal. Comparou-se a
produ\ue7\ue3o de biomassa e ciclagem de nutrientes no SAF, com a
leguminosa herb\ue1cea Pueraria phaseoloides e aduba\ue7\ue3o
nitrogenada. O SAF foi implantado em maio de 2004, no Campo
Experimental da Embrapa Agrobiologia, em Serop\ue9dica - RJ. O
delineamento experimental foi de blocos casualizados com cinco
tratamentos, constitu\ueddos das leguminosas
ac\ue1cia-angust\uedssima (Acacia angustissima), kudzu-tropical (
Pueraria phaseoloides, gliric\ueddia (Gliricidia sepium) dispostas
nas entrelinhas da banana e do a\ue7a\ued; al\ue9m de
aduba\ue7\ue3o nitrogenada com ureia, e cobertura viva oriunda da
vegeta\ue7\ue3o espont\ue2nea. Para quantificar a
produ\ue7\ue3o de biomassa e libera\ue7\ue3o de N, P, Ca, Mg e
K in situ foram cortadas as leguminosas e ro\ue7ado o kudzu-tropical
e a vegeta\ue7\ue3o espont\ue2nea. As determina\ue7\uf5es da
mat\ue9ria seca remanescente, libera\ue7\ue3o de nutrientes,
constantes de decomposi\ue7\ue3o e os tempos de meia-vida dos
res\uedduos vegetais foram realizadas acondicionando-se 50 g de
material fresco em litterbags, dispostos na superf\uedcie do solo,
tomando-se amostras aos 0, 5, 10, 15, 20, 25, 30, 40, 60 e 75 ap\uf3s
o in\uedcio do experimento. Acacia angustissima e kudzu-tropical
apresentaram maior produ\ue7\ue3o de biomassa seca, igual a 10,8 Mg
ha-1. Os res\uedduos da gliric\ueddia resultaram em maiores
constantes de decomposi\ue7\ue3o, nas duas esta\ue7\uf5es do
ano.The Agroforestry Systems (SAFs) bring multiple benefits and they are an
alternative to minimize environmental degradation, and to achieve a
sustainable development, due to greatest diversity of species. This
study evaluated the contribution of the leguminous trees,
Gliric\ueddia sepium and Acacia angustissima , grown in alley
cropping of banana (Musa sp.) and \u201ca\ue7a\ued\u201d palm (
Euterpe oleraceae ) used as green manure in the implantation of an
Agroforestry Systems. They were compared the production of biomass,
nutrients cycling, nitrogen intake, activity and diversity of soil
fauna, and banana productivity in the SAF, and with the usage of the
legume Pueraria phaseoloides and nitrogen fertilization. The SAF
implantation occurred in May 2004, at the Research Center of Embrapa
Agrobiologia, in Serop\ue9dica, Rio de Janeiro State. The following
year it was planted the forest African mahogany specie ( Kaya
senegalensis ), at the centre of the legumes alleys. The experimental
design was of randomized blocks with five treatments and four
repetitions. The treatments consisted of the leguminous trees arranged
between the lines of bananas and the \u201ca\ue7a\ued\u201d palm,
and they were: acacia angust\uedssima (Acacia angustissima), tropical
kudzu (Pueraria phaseoloides), and gliric\ueddia (Gliric\ueddia
sepium); besides application of nitrogen as urea and spontaneous
vegetation. To quantify the production of biomass, and the release of
N, P, Ca, Mg and K, the legumes branches were cut and the kudzu
tropical and spontaneous vegetation were mowed, in the rainy and dry
seasons. The determination of remaining dry matter, releasing of
nutrients, decomposition rates, and half life time of plant residues
were held to 50 grams of fresh material from litterbags, placed on the
soil surface, sampled at 0, 5, 10, 15, 20, 25, 30, 40, 60 and 75 days
after the installation of the experiment. Acacia angustissima and kudzu
tropical showed higher dry biomass, 9.5 and 10.8 Mg ha-1, respectively.
The gliric\ueddia residues showed the highest decomposition rates, in
the two year seasons
Pulsed ultrasound associated with gold nanoparticle gel reduces oxidative stress parameters and expression of pro-inflammatory molecules in an animal model of muscle injury
MarinEye - A tool for marine monitoring
This work presents an autonomous system for marine integrated physical-chemical and biological monitoring – the MarinEye system. It comprises a set of sensors providing diverse and relevant information for oceanic environment characterization and marine biology studies. It is constituted by a physicalchemical water properties sensor suite, a water filtration and sampling system for DNA collection, a plankton imaging
system and biomass assessment acoustic system. The MarinEye system has onboard computational and
logging capabilities allowing it either for autonomous operation or for integration in other marine observing systems (such as Observatories or robotic vehicles. It was designed in order to collect integrated multi-trophic monitoring data. The validation in operational environment on 3 marine observatories: RAIA, BerlengasWatch and Cascais on the coast of Portugal is also discussed.info:eu-repo/semantics/publishedVersio
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