Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct

We tested whether severe congestive heart failure (CHF), a condition associated with excess free-water retention, is accompanied by altered regulation of the vasopressin-regulated water channel, aquaporin-2 (AQP2), in the renal collecting duct. CHF was induced by left coronary artery ligation. Compared with sham-operated animals, rats with CHF had severe heart failure with elevated left ventricular end-diastolic pressures (LVEDP): 26.9 ± 3.4 vs. 4.1 ± 0.3 mmHg, and reduced plasma sodium concentrations (142.2 ± 1.6 vs. 149.1 ± 1.1 mEq/liter). Quantitative immunoblotting of total kidney membrane fractions revealed a significant increase in AQP2 expression in animals with CHF (267 ± 53%, n=12) relative to sham-operated controls (100 ± 13%, n=14). In contrast, immunoblotting demonstrated a lack of an increase in expression of AQP1 and AQP3 water channel expression, indicating that the effect on AQP2 was selective.Furthermore, postinfarction animals without LVEDP elevation or plasma Na reduction showed no increase in AQP2 expression (121 ± 28% of sham levels, n=6). Immunocytochemistry and immunoelectron microscopy demonstrated very abundant labeling of the apical plasma membrane and relatively little labeling of intracellular vesicles in collecting duct cells from rats with severe CHF, consistent with enhanced trafficking of AQP2 to the apical plasma membrane. The selective increase in AQP2 expression and enhanced plasma membrane targeting provide an explanation for the development of water retention and hyponatremia in severe CHF

Archaeological Investigations of Areas Slated for Expansion at Fort Sam Houston National Cemetery, San Antonio, Texas

During April 1982, archaeologists from the Center for Archaeological Research I at The University of Texas at San Antonio conducted a cultural resource survey and evaluation of 31.68 acres slated as an expansion area for the Fort Sam Houston National Cemetery in San Antonio, Texas. This was accomplished through two research methods: (1) a literature and archival search supplemented by interviews of knowledgeable persons; followed by (2) a planned subsurface archaeological testing program. One badly disturbed prehistoric site (41 BX 346) of unknown function and unknown chronological association was discovered as a result of these activities. Because the site is so severely mixed and displaced, it is not considered a significant cultural resource, and no further work is recommended. The legal basis for this cultural resource survey and evaluation is described in the federal legislation of the National Historic Preservation Act of 1966, as amended, the Archeological and Historical Preservation Act of 1974 and Executive Order 11593

Reconstruction Control of Magnetic Properties during Epitaxial Growth of Ferromagnetic Mn_3-δGa on Wurtzite GaN(0001)

Binary ferromagnetic Mn_3-δGa (1.2<3-δ≤1.5) crystalline thin films have been epitaxially grown on wurtzite GaN(0001) surfaces using rf N-plasma molecular beam epitaxy. The film structure is face-centered tetragonal with CuAu type-I (L1_0) ordering with (111) orientation. The in-plane epitaxial relationship to GaN is nearly ideal with [11̅ 0]_MnGa∥[11̅ 00]_GaN and [112̅ ]_MnGa∥[112̅ 0]_GaN. We observe magnetic anisotropy along both the in-plane and out-of-plane directions. The magnetic moments are found to depend on the Mn/(Mn+Ga) flux ratio and can be controlled by observation of the surface reconstruction during growth, which varies from 1×1 to 2×2 with increasing Mn stoichiometry

Applicability of remote sensor data to geologic analysis of the Bonanza test site Colorado

There are no author-identified significant results in this report

Village Water Ozonation System

The Village Water Ozonation System (VWOS) team’s core mission statement is to provide economically sustainable and culturally sensitive drinking water solutions for communities, to empower communities with the ability to properly maintain their drinking water supply, and to transform people’s lives by decreasing the occurrences of waterborne diseases. Currently, the VWOS team is partnering with Friends in Action to design and implement two drinking water treatment systems for the community living on Rama Cay, an island in the Bluefields Lagoon on the eastern coastline of Nicaragua. The wells on the island are contaminated with E. coli and other bacteria and contain high levels of salt that cause the water to be unhealthy, distasteful, and corrosive to metal equipment in the system. The team hopes to design a system that will disinfect the water, remove salinity from the well water with a safe and efficient disposal of all byproducts, and decrease corrosion agents. VWOS is partnering with Forward Edge International for the third time (Nicaragua 2009 and Mexico 2016) to design water treatment systems for communities in Oaxaca, Mexico and Kijabe, Kenya. The system for Oaxaca is ready for implementation and awaits availability to travel. The system for Kijabe is in the initial stage of communicating with the client on specifics for the design.https://mosaic.messiah.edu/engr2021/1018/thumbnail.jp

Key Lessons Learned from Moffitt's Molecular Tumor Board: The Clinical Genomics Action Committee Experience

The increasing practicality of genomic sequencing technology has led to its incorporation into routine clinical practice. Successful identification and targeting of driver genomic alterations that provide proliferative and survival advantages to tumor cells have led to approval and ongoing development of several targeted cancer therapies. Within many major cancer centers, molecular tumor boards are constituted to shepherd precision medicine into clinical practice

The Lantern Vol. 15, No. 1, Fall 1946

• Public, Speaking • Concept • The Storm • Yes Sir! • Messengers of Death • The Anonymous Letter • Best Trust the Happy Moments • Disillusionment • The Man With the Water-Brown Eyes • Poetry • Who Knows?https://digitalcommons.ursinus.edu/lantern/1040/thumbnail.jp

Multi-scale assembly with robot teams

In this paper we present algorithms and experiments for multi-scale assembly of complex structures by multi-robot teams. We also focus on tasks where successful completion requires multiple types of assembly operations with a range of precision requirements. We develop a hierarchical planning approach to multi-scale perception in support of multi-scale manipulation, in which the resolution of the perception operation is matched with the required resolution for the manipulation operation. We demonstrate these techniques in the context of a multi-step task where robots assemble large box-like objects, inspired by the assembly of an airplane wing. The robots begin by transporting a wing panel, a coarse manipulation operation that requires a wide field of view, and gradually shifts to a narrower field of view but with more accurate sensors for part alignment and fastener insertion. Within this framework we also provide for failure detection and recovery: upon losing track of a feature, the robots retract to using wider field of view systems to re-localize. Finally, we contribute collaborative manipulation algorithms for assembling complex large objects. First, the team of robots coordinates to transport large assembly parts which are too heavy for a single robot to carry. Second, the fasteners and parts are co-localized for robust insertion and fastening. We implement these ideas using four KUKA youBot robots and present experiments where our robots successfully complete all 80 of the attempted fastener insertion operations