831 research outputs found
Atmospheric Calorimetry above 10 eV: Shooting Lasers at the Pierre Auger Cosmic-Ray Observatory
The Pierre Auger Cosmic-Ray Observatory uses the earth's atmosphere as a
calorimeter to measure extensive air-showers created by particles of
astrophysical origin. Some of these particles carry joules of energy. At these
extreme energies, test beams are not available in the conventional sense. Yet
understanding the energy response of the observatory is important. For example,
the propagation distance of the highest energy cosmic-rays through the cosmic
microwave background radiation (CMBR) is predicted to be strong function of
energy. This paper will discuss recently reported results from the observatory
and the use of calibrated pulsed UV laser "test-beams" that simulate the
optical signatures of ultra-high energy cosmic rays. The status of the much
larger 200,000 km companion detector planned for the northern hemisphere
will also be outlined.Comment: 6 pages, 11 figures XIII International Conference on Calorimetry in
High Energy Physic
Description of Atmospheric Conditions at the Pierre Auger Observatory Using Meteorological Measurements and Models
Atmospheric conditions at the site of a cosmic ray observatory must be known
well for reconstructing observed extensive air showers, especially when
measured using the fluorescence technique. For the Pierre Auger Observatory, a
sophisticated network of atmospheric monitoring devices has been conceived.
Part of this monitoring was a weather balloon program to measure atmospheric
state variables above the Observatory. To use the data in reconstructions of
air showers, monthly models have been constructed. Scheduled balloon launches
were abandoned and replaced with launches triggered by high-energetic air
showers as part of a rapid monitoring system. Currently, the balloon launch
program is halted and atmospheric data from numerical weather prediction models
are used. A description of the balloon measurements, the monthly models as well
as the data from the numerical weather prediction are presented
An update on the genetics of age-related macular degeneration
Age-related macular degeneration (AMD) is a genetically complex disorder of the photoreceptor-RPE-Bruch's membrane-choriocapillaris complex. Family and twin studies have shown that the susceptibility for this disease is genetically influenced. The heritability has been estimated to be up to 71%. Linkage and association studies have identified several chromosomal regions that are likely to contain susceptibility loci with strongest evidence found on chromosome 1q31 and 10q26. Variants in the complement factor H (CFH) gene have been shown by several independent studies to be associated with an increased risk for AMD in Caucasian populations. These findings imply that the innate immune system may play a significant role in AMD pathogenesis. The LOC387715/HTRA1 locus within 10q26 has been identified as a second major locus contributing to AMD pathogenesis. The two late forms of AMD, choroidal neovascularization and geographic atrophy, have not been found to be different in risk allele distribution. Variants within CFH and LOC387715/HTRA1 may contribute to the increased risk of late AMD largely through their impact on precursors, such as drusen and/or other RPE/Bruch's membrane changes. Considering variants at CFH, LOC387715/HTRA1 and complement component 2-complement factor B (C2-FB), high-risk homozygotes at all three loci may have a 250-fold increased risk compared to baseline. However, the identification of genetic factors has not resulted in therapeutic strategies to modify the disease so far and additional genetic and environmental factors are yet to be discovered in order to influence the onset and the progression of AMD
Air fluorescence measurements in the spectral range 300-420 nm using a 28.5 GeV electron beam
Measurements are reported of the yield and spectrum of fluorescence, excited
by a 28.5 GeV electron beam, in air at a range of pressures of interest to
ultra-high energy cosmic ray detectors. The wavelength range was 300 - 420 nm.
System calibration has been performed using Rayleigh scattering of a nitrogen
laser beam. In atmospheric pressure dry air at 304 K the yield is 20.8 +/- 1.6
photons per MeV.Comment: 29 pages, 10 figures. Submitted to Astroparticle Physic
A novel method for the absolute fluorescence yield measurement by AIRFLY
One of the goals of the AIRFLY (AIR FLuorescence Yield) experiment is to
measure the absolute fluorescence yield induced by electrons in air to better
than 10% precision. We introduce a new technique for measurement of the
absolute fluorescence yield of the 337 nm line that has the advantage of
reducing the systematic uncertainty due to the detector calibration. The
principle is to compare the measured fluorescence yield to a well known process
- the Cerenkov emission. Preliminary measurements taken in the BFT (Beam Test
Facility) in Frascati, Italy with 350 MeV electrons are presented. Beam tests
in the Argonne Wakefield Accelerator at the Argonne National Laboratory, USA
with 14 MeV electrons have also shown that this technique can be applied at
lower energies.Comment: presented at the 5th Fluorescence Workshop, El Escorial - Madrid,
Spain, 16 - 20 September 200
Temperature and Humidity Dependence of Air Fluorescence Yield measured by AIRFLY
The fluorescence detection of ultra high energy cosmic rays requires a
detailed knowledge of the fluorescence light emission from nitrogen molecules
over a wide range of atmospheric parameters, corresponding to altitudes typical
of the cosmic ray shower development in the atmosphere. We have studied the
temperature and humidity dependence of the fluorescence light spectrum excited
by MeV electrons in air. Results for the 313.6 nm, 337.1 nm, 353.7 nm and 391.4
nm bands are reported in this paper. We found that the temperature and humidity
dependence of the quenching process changes the fluorescence yield by a
sizeable amount (up to 20%) and its effect must be included for a precise
estimation of the energy of ultra high energy cosmic rays.Comment: presented at the 5th Fluorescence Workshop, El Escorial - Madrid,
Spain, 16 - 20 September 2007, to appear in Nuclear Instruments and Methods
Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest differentiation
Replacement of canonical histones with specialized histone variants promotes altering of chromatin structure and function. The essential histone variant H2A.Z affects various DNA-based processes via poorly understood mechanisms. Here, we determine the comprehensive interactome of H2A.Z and identify PWWP2A as a novel H2A.Z-nucleosome binder. PWWP2A is a functionally uncharacterized, vertebrate-specific protein that binds very tightly to chromatin through a concerted multivalent binding mode. Two internal protein regions mediate H2A.Z-specificity and nucleosome interaction, whereas the PWWP domain exhibits direct DNA binding. Genome-wide mapping reveals that PWWP2A binds selectively to H2A.Z-containing nucleosomes with strong preference for promoters of highly transcribed genes. In human cells, its depletion affects gene expression and impairs proliferation via a mitotic delay. While PWWP2A does not influence H2A.Z occupancy, the C-terminal tail of H2A.Z is one important mediator to recruit PWWP2A to chromatin. Knockdown of PWWP2A in Xenopus results in severe cranial facial defects, arising from neural crest cell differentiation and migration problems. Thus, PWWP2A is a novel H2A.Z-specific multivalent chromatin binder providing a surprising link between H2A.Z, chromosome segregation, and organ development
PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex.
Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels
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