Overestimation of the 25OHD serum concentration with the automated IDS EIA kit.

We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit

Statistical practices of educational researchers: An analysis of their ANOVA, MANOVA, and ANCOVA analyses

Articles published in several prominent educational journals were examined to investigate the use of data-analysis tools by researchers in four research paradigms: between-subjects univariate designs, between-subjects multivariate designs, repeated measures designs, and covariance designs. In addition to examining specific details pertaining to the research design (e.g., sample size, group size equality/inequality) and methods employed for data analysis, we also catalogued whether: (a) validity assumptions were examined, (b) effect size indices were reported, (c) sample sizes were selected based on power considerations, and (d) appropriate textbooks and/or articles were cited to communicate the nature of the analyses that were performed. Our analyses imply that researchers rarely verify that validity assumptions are satisfied and accordingly typically use analyses that are nonrobust to assumption violations. In addition, researchers rarely report effect size statistics, nor do they routinely perform power analyses to determine sample size requirements. We offer many recommendations to rectify these shortcomings.Social Sciences and Humanities Research Counci

The opposite of Dante's hell? The transfer of ideas for social housing at international congresses in the 1850s–1860s

With the advent of industrialization, the question of developing adequate housing for the emergent working classes became more pressing than before. Moreover, the problem of unhygienic houses in industrial cities did not stop at the borders of a particular nation-state; sometimes literally as pandemic diseases spread out 'transnationally'. It is not a coincidence that in the nineteenth century the number of international congresses on hygiene and social topics expanded substantially. However, the historiography about social policy in general and social housing in particular, has often focused on individual cases because of the different pace of industrial and urban development and is thus dominated by national perspectives. In this paper, I elaborate on transnational exchange processes and local adaptations and transformations. I focus on the transfer of the housing model of SOMCO in Mulhouse, (a French house building association) during social international congresses. I examine whether cross-national networking enabled and facilitated the implementation of ideas on the local scale. I will elaborate on the transmission and the local adaptation of the Mulhouse-model in Belgium. Convergences, divergences, and different factors that influenced the local transformations (personal choice, political situation, socioeconomic circumstances) will be taken into accoun

Physical activity mass media campaigns and their evaluation: a systematic review of the literature 2003–2010

Internationally, mass media campaigns to promote regular moderate-intensity physical activity have increased recently. Evidence of mass media campaign effectiveness exists in other health areas, however the evidence for physical activity is limited. The purpose was to systematically review the literature on physical activity mass media campaigns, 2003–2010. A focus was on reviewing evaluation designs, theory used, formative evaluation, campaign effects and outcomes. Literature was searched resulting in 18 individual adult mass media campaigns, mostly in high-income regions and two in middle-income regions. Designs included: quasi experimental (n = 5); non experimental (n = 12); a mixed methods design (n = 1). One half used formative research. Awareness levels ranged from 17 to 95%. Seven campaigns reported significant increases in physical activity levels. The review found that beyond awareness raising, changes in other outcomes were measured, assessed but reported in varying ways. It highlighted improvements in evaluation, although limited evidence of campaign effects remain. It provides an update on the evaluation methodologies used in the adult literature. We recommend optimal evaluation design should include: (1) formative research to inform theories/frameworks, campaign content and evaluation design; (2) cohort study design with multiple data collection points; (3) sufficient duration; (4) use of validated measures; (5) sufficient evaluation resources

Expression profiling of blood samples from an SU5416 Phase III metastatic colorectal cancer clinical trial: a novel strategy for biomarker identification

BACKGROUND: Microarray-based gene expression profiling is a powerful approach for the identification of molecular biomarkers of disease, particularly in human cancers. Utility of this approach to measure responses to therapy is less well established, in part due to challenges in obtaining serial biopsies. Identification of suitable surrogate tissues will help minimize limitations imposed by those challenges. This study describes an approach used to identify gene expression changes that might serve as surrogate biomarkers of drug activity. METHODS: Expression profiling using microarrays was applied to peripheral blood mononuclear cell (PBMC) samples obtained from patients with advanced colorectal cancer participating in a Phase III clinical trial. The PBMC samples were harvested pre-treatment and at the end of the first 6-week cycle from patients receiving standard of care chemotherapy or standard of care plus SU5416, a vascular endothelial growth factor (VEGF) receptor tyrosine kinase (RTK) inhibitor. Results from matched pairs of PBMC samples from 23 patients were queried for expression changes that consistently correlated with SU5416 administration. RESULTS: Thirteen transcripts met this selection criterion; six were further tested by quantitative RT-PCR analysis of 62 additional samples from this trial and a second SU5416 Phase III trial of similar design. This method confirmed four of these transcripts (CD24, lactoferrin, lipocalin 2, and MMP-9) as potential biomarkers of drug treatment. Discriminant analysis showed that expression profiles of these 4 transcripts could be used to classify patients by treatment arm in a predictive fashion. CONCLUSIONS: These results establish a foundation for the further exploration of peripheral blood cells as a surrogate system for biomarker analyses in clinical oncology studies

Analysis of parcel-based image classification methods for monitoring the activities of the Land Bank of Galicia (Spain)

[EN] The abandonment of agricultural plots entails a low economic productivity of the land and a higher vulnerability to wildfires and degradation of affected areas. In this sense, the local government of Galicia is promoting new methodologies based on high-resolution images in order to classify the territory in basic and generic land uses. This procedure will be used to control the sustainable management of plots belonging to the Land Bank. This paper presents an application study for maintaining and updating land use/land cover geospatial databases using parcel-oriented classification. The test is performed over two geographic areas of Galicia, in the northwest of Spain. In this region, forest and shrublands in mountain environments are very heterogeneous with many private unproductive plots, some of which are in a high state of abandonment. The dataset is made of high spatial resolution multispectral imagery, cadastral cartography employed to define the image objects (plots), and field samples used to define evaluation and training samples. A set of descriptive features is computed quantifying different properties of the objects, i.e. spectral, texture, structural, and geometrical. Additionally, the effect on the classification and updating processes of the historical land use as a descriptive feature is tested. Three different classification methodologies are analyzed: linear discriminant analysis, decision trees, and support vector machine. The overall accuracies of the classifications obtained are always above 90 % and support vector machine method is proved to provide the best performance. Forest and shrublands areas are especially undefined, so the discrimination between these two classes is low. The results enable to conclude that the use of automatic parcel-oriented classification techniques for updating tasks of land use/land cover geospatial databases, is effective in the areas tested, particularly when broad and well defined classes are required.The authors appreciate the collaboration and support provided by Xunta de Galicia, Sociedade para o Desenvolvemento Comarcal de Galícia, and Banco de Terras de Galicia. The financial support provided by the Spanish Ministerio de Ciencia e Innovación in the framework of the projects CGL2010-19591/BTE and CGL2009-14220 is also acknowledged.Hermosilla, T.; Díaz Manso, J.; Ruiz Fernández, LÁ.; Recio Recio, JA.; Fernández-Sarría, A.; Ferradáns Nogueira, P. (2012). Analysis of parcel-based image classification methods for monitoring the activities of the Land Bank of Galicia (Spain). Applied Geomatics. 4(4):245-255. https://doi.org/10.1007/s12518-012-0087-zS24525544Arikan M (2004) Parcel-based crop mapping through multi-temporal masking classification of landsat 7 images in Karacabey, Turkey. Int Arch Photogramm Remote Sens Spat Inf Sci 35:1085–1090Balaguer A, Ruiz LA, Hermosilla T, Recio JA (2010) Definition of a comprehensive set of texture semivariogram features and their evaluation for object-oriented image classification. Comput Geosci 36(2):231–240Balaguer-Besser A, Hermosilla T, Recio JA, Ruiz LA (2011) Semivariogram calculation optimization for object-oriented image classification. Model Sci Educ Learn 4(7):91–104Blaschke T (2010) Object based image analysis for remote sensing. ISPRS J Photogramm 65(1):2–16Cohen Y, Shoshany M (2000) Integration of remote sensing, GIS and expert knowledge in national knowledge-based crop recognition in Mediterranean environment. Int Arch Photogramm Remote Sens 33(Part B7):280–286Congalton R (1991) A review of assessing the accuracy of classifications of remotely sensed data. Remote Sens Environ 37(1):35–46Dadhwal VK, Singh RP, Dutta S, Parihar JS (2002) Remote sensing based crop inventory: a review of Indian experience. Trop Ecol 43(1):107–122De Wit AJW, Clevers JGPW (2004) Efficiency and accuracy of per-field classification for operational crop mapping. Int J Remote Sens 25:4091–4112Del Frate F, Pacifici F, Solimini D (2008) Monitoring urban land cover in Rome, Italy, and its changes by single-polarization multitemporal SAR images. IEEE J Sel Top Appl Earth Obs Remote Sens 1:87–97Díaz-Manso JM, Ferradáns-Nogueira P (2011) Modelo de uso actual da terra. In: Cobelle-Rico EJ, Diaz-Manso JM, Crecente-Maseda R, Martínez-Rivas EM (eds) Mercado e Mobilidade de Terras en Galícia, 1st edn. Servizo de Publicacións e Intercambio Científico, Santiago de Compostela, Spain, pp 31–44Dupas CA (2000) SAR and LANDSAT TM image fusion for land cover classification in the Brazilian Atlantic Forest Domain. Int Arch Photogramm Remote Sens XXXIII(Part B1):96–103El Kady M, Mack CB (1992) Remote sensing for crop inventory of Egypt’s old agricultural lands. Int Arch Photogramm Remote Sens 29:176–185Everitt BS, Dunn G (2001) Applied multivariate data analysis, 2nd edn. Edward Arnold, LondonHaralick RM, Shanmugam K, Dinstein I (1973) Texture features for image classification. IEEE Transact Syst Man Cybern 3(6):610–622Hermosilla T, Almonacid J, Fernández-Sarría A, Ruiz LA, Recio JA (2010) Combining features extracted from imagery and lidar data for object-oriented classification of forest areas. Int Arch Photogramm Remote Sens Spat Inf Sci 38(4/C7)Hernández Orallo J, Ramírez Quintana MJ, Ferri Ramírez C (2004) Introducción a la minería de datos. Pearson Educación S.A, MadridHomer C, Huang C, Yang L, Wylie B, Coan M (2004) Development of a 2001 National Land-Cover Database for the United States. Photogramm Eng Remote Sens 70:829–840Huberty CJ (1994) Applied discriminant analysis. Wiley, New YorkLaws KI (1985) Goal-directed texture image segmentation. Appl Artif Intel II, SPIE 548:19–26Ormeci C, Alganci U, Sertel E (2010) Identification of crop areas using SPOT-5 data, FIG Congress 2010 Facing the Challenges—building the capacity. Sydney, Australia, pp 11–16Peled A, Gilichinsky M (2004) GIS-driven analyses of remotely sensed data for quality assessment of existing land cover classification. Int Arch Photogramm Remote Sens Spat Inf Sci 35Peled A, Gilichinsky M (2010) Knowledge-based classification of land cover for the quality assessment of GIS database. Int Arch Photogramm Remote Sens Spat Inf Sci 38:217–222Perveen F, Nagasawa R, Ali S, Husnain (2008) Evaluation of ASTER spectral bands for agricultural land cover mapping using pixel-based and object-based classification approaches. Int Arch Photogramm Remote Sens Spat Inf Sci 37(4-C1)Petit CC, Lambin EF (2002) Impact of data integration technique on historical land-use/land-cover change: comparing historical maps with remote sensing data in the Belgian Ardennes. Landsc Ecol 17:117–132Quinlan JR (1993) C4.5: Programs for machine learning. Kaufmann, San FranciscoRabe A, van der Linden S, Hostert P (2010) imageSVM, Version 2.1. www.hu-geomatics.deRecio JA, Hermosilla T, Ruiz LA, Fernández-Sarría A (2011) Historical land use as a feature for image classification. Photogramm Eng Remote Sens 77(4):377–387Ruiz LA, Fernández-Sarría A, Recio JA (2004) Texture feature extraction for classification of remote sensing data using wavelet decomposition: a comparative study. Int Arch Photogramm Remote Sens Spat Inf Sci 35(B4):1109–1115Ruiz LA, Recio JA, Hermosilla T, Fdez. Sarriá A (2009) Identification of agricultural and land cover database changes using object-oriented classification techniques. 33rd International Symposium on Remote Sensing of Environment, May 4–8, Stresa (Italy)Ruiz LA, Recio JA, Fernández-Sarría A, Hermosilla T (2011) A feature extraction software tool for agricultural object-based image analysis. Comput Electron Agric 76(4):284–296Tansey K, Chambers I, Anstee A, Denniss A, Lamb A (2009) Object-oriented classification of very high resolution airborne imagery for the extraction of hedgerows and field margin cover in agricultural areas. Appl Geogr 29(2):145–157van der Linden S, Rabe A, Wirth F, Suess S, Okujeni A, Hostert P (2010) imageSVM regression, application manual: imageSVM version 2.1. Humboldt-Universität zu Berlin, GermanyVapnik VN (1998) Statistical learning theory. Wiley, New YorkWalsh SJ, McCleary AL, Mena CF, Shao Y, Tuttle JP, Gonzalez A, Atkinson R (2008) QuickBird and Hyperion data analysis of an invasive plant species in the Galapagos Islands of Ecuador: implications for control and land use management. Remote Sens Environ 112(5):1927–1941Walter V (2004) Object-based classification of remote sensing data for change detection. ISPRS J Photogramm Remote Sens 58:225–238Walter V (2005) Object-based evaluation of lidar and multiespectral data for automatic change detection in GIS databases. Geo-Inf Syst 18:10–15Zaragozí, B, Rabasa, A, Rodríguez-Sala, JJ, Navarro, JT, Belda, A, Ramón, A (2012) Modelling farmland abandonment: A study combining GIS and data mining techniques. Agric Ecosys Environ 155:124–132Zhang S, Liu X (2005) Realization of data mining model for expert classification using multi-scale spatial data. Int Arch Photogramm Remote Sens Spat Inf Sci 26(4/W6):107–11

Community led active schools programme (CLASP) exploring the implementation of health interventions in primary schools: headteachers’ perspectives

Background: Schools are repeatedly utilised as a key setting for health interventions. However, the translation of effective research findings to the school setting can be problematic. In order to improve effective translation of future interventions, it is imperative key challenges and facilitators of implementing health interventions be understood from a school's perspective. Methods: Nineteen semi-structured interviews were conducted in primary schools (headteachers n = 16, deputy headteacher n = 1, healthy school co-ordinator n = 2). Interviews were transcribed verbatim and analysed using thematic analysis. Results: The main challenges for schools in implementing health interventions were; government-led academic priorities, initiative overload, low autonomy for schools, lack of staff support, lack of facilities and resources, litigation risk and parental engagement. Recommendations to increase the application of interventions into the school setting included; better planning and organisation, greater collaboration with schools and external partners and elements addressing sustainability. Child-centred and cross-curricular approaches, inclusive whole school approaches and assurances to be supportive of the school ethos were also favoured for consideration. Conclusions: This work explores schools' perspectives regarding the implementation of health interventions and utilises these thoughts to create guidelines for developing future school-based interventions. Recommendations include the need to account for variability between school environments, staff and pupils. Interventions with an element of adaptability were preferred over the delivery of blanket fixed interventions. Involving schools in the developmental stage would add useful insights to ensure the interventions can be tailored to best suit each individual schools' needs and improve implementation.11 page(s

Tuberculosis incidence in foreign-born people residing in European countries in 2020.

BackgroundEuropean-specific policies for tuberculosis (TB) elimination require identification of key populations that benefit from TB screening.AimWe aimed to identify groups of foreign-born individuals residing in European countries that benefit most from targeted TB prevention screening.MethodsThe Tuberculosis Network European Trials group collected, by cross-sectional survey, numbers of foreign-born TB patients residing in European Union (EU) countries, Iceland, Norway, Switzerland and the United Kingdom (UK) in 2020 from the 10 highest ranked countries of origin in terms of TB cases in each country of residence. Tuberculosis incidence rates (IRs) in countries of residence were compared with countries of origin.ResultsData on 9,116 foreign-born TB patients in 30 countries of residence were collected. Main countries of origin were Eritrea, India, Pakistan, Morocco, Romania and Somalia. Tuberculosis IRs were highest in patients of Eritrean and Somali origin in Greece and Malta (both > 1,000/100,000) and lowest among Ukrainian patients in Poland (3.6/100,000). They were mainly lower in countries of residence than countries of origin. However, IRs among Eritreans and Somalis in Greece and Malta were five times higher than in Eritrea and Somalia. Similarly, IRs among Eritreans in Germany, the Netherlands and the UK were four times higher than in Eritrea.ConclusionsCountry of origin TB IR is an insufficient indicator when targeting foreign-born populations for active case finding or TB prevention policies in the countries covered here. Elimination strategies should be informed by regularly collected country-specific data to address rapidly changing epidemiology and associated risks