Disorders of the immune status in patients with chronic cerebral ischemia; differentiated pharmacological correction

The objective of the study is to establish the immune disorder patterns in patients with CCI I-II associated with arterial hypertension and to develop differentiated pharmacological methods for their correctio

Bacterial Carriage of Pathogenic Antibiotic-resistant Staphylococci Among Conditionally Healthy Infants and Preschool-age Children

Staphylococci are related to opportunistic microorganisms and are part of normal human microflora. However, there are varieties that can cause pyoinflammatory diseases in organisms with weakened immune status. In the event of an inadequate therapeutic approach, they are preserved in the body, and the person becomes a bacterial carrier and a source of dissemination of pathogenic microorganisms. The formation of antibiotic resistance also plays a significant role in the emergence of bacterial carriage. The situation is especially serious in organized children’s groups, where children are in close contact with one another. Thus, the study of the prevalence rate of antibiotic resistance among pathogenic strains of staphylococci in children’s groups is topical and worthy of attention. The purpose of this work is to identify carriers of pathogenic and antibiotic resistant staphylococci isolated from the microflora of the oral cavity of preschool children considered healthy. The results of the research can prove useful in practical healthcare and, particularly, in pediatric practice. Keywords: staphylococci, pathogenic cocci, bacterial carriage, antibiotic resistance, infant morbidity rat

Desarrollo de habilidades creativas de los estudiantes en instituciones de educación superior

main purpose of vocational training in higher education is training of a competitive highly qualified graduate. The requirements for his professional and creative level of development increase with increasing role of creative abilities in professional activities implementation. The purpose of the article is to describe experience of students’ creative abilities development. The authors identified the stages of students’ creative abilities development: organizational and preparatory; activity-evaluation; designing, during which students develop original projects, develop creative abilities and readiness for creative activity. The article describes the experience of project training in the branch of Tyumen industrial University in Surgut. The stages of project preparation "Drilling rig, combined with installation of a rod depth pump (hereinafter –RSP) are analyzed in detail. The paper also provides examples of project tasks performed by students in the study of pedagogical courses. Criteria for evaluating creative abilities were developed. In order to achieve high results, students plan their own individual activities and team activities in detail, make non-standard decisions during the project, show flexibility in resolving conflicts and originality of ideas. Modern high school, acting in terms of competence approach, using project activities, contributes to developing an independent and creative personality of future professional.El objetivo principal de la formación profesional en la educación superior es la formación de un graduado competitivo altamente calificado. Los requisitos para su nivel de desarrollo profesional y creativo aumentan con el papel cada vez mayor de las habilidades creativas en la implementación de actividades profesionales. El propósito del artículo es describir la experiencia del desarrollo de habilidades creativas de los estudiantes. Los autores identificaron las etapas del desarrollo de las habilidades creativas de los estudiantes: organizacionales y preparatorias; evaluación de actividad; diseño, durante el cual los estudiantes desarrollan proyectos originales, desarrollan habilidades creativas y preparación para la actividad creativa. El artículo describe la experiencia de capacitación en proyectos en la rama de la Universidad industrial de Tyumen en Surgut. Las etapas de preparación del proyecto "Plataforma de perforación, combinadas con la instalación de una bomba de profundidad de varilla (en adelante, RSP) se analizan en detalle. El documento también proporciona ejemplos de tareas de proyectos realizadas por estudiantes en el estudio de cursos pedagógicos. Criterios para evaluar habilidades creativas se desarrollaron. Con el fin de lograr altos resultados, los estudiantes planean sus propias actividades individuales y actividades de equipo en detalle, toman decisiones no estándar durante el proyecto, muestran flexibilidad para resolver conflictos y originalidad de ideas. Preparatoria moderna, actuando en términos de competencia El enfoque, utilizando actividades de proyecto, contribuye al desarrollo de una personalidad independiente y creativa del futuro profesional

Organization of virtual interaction in the context of the coronavirus pandemic

The organization of the educational process should be carried out taking into account the epidemiological situation and following the requirements that are due to quarantine measures. The leadership of vocational schools is looking for ways to conduct classes in a safe environment, assessing the potential risks for teachers and students. Higher schools implement the possibilities of electronic technologies as a factor in ensuring the safety of subjects of the educational process for communication between students and teachers at a distance. The purpose of the article is to review the experience of organizing virtual interaction between students and teachers within the framework of e-learning during the spread of coronavirus infection. The article reveals the possibilities of electronic technologies that allow communicating at a remote distance. The preparation of students in critical conditions is carried out using various electronic means that promote active interaction. The results of the study reflect the opinion of students of higher educational institutions about the use of virtual interaction tools. The results obtained using the Likert scale allow us to say that the majority of the respondents are active participants in virtual interaction and are ready to continue to communicate using the presented means despite the existing difficulties

Capacitación interna para empleados de empresas de servicios que utilizan tecnología de la información

The article reveals the structure of the content of in-house training and retraining of employees, reflects the principles on which training is based and the functions that are performed by in-house professional training. Throughout the world and in Russia in particular, the subject of increased attention is the introduction of information technology. Therefore, the authors consider the in-house training of employees of the service company using the designated technologies. The agency Registration Agency, a limited liability company, was considered in the article. In 2018, among its employees, a survey was conducted that revealed the desire of employees to improve their skills through electronic courses provided by Nizhny Novgorod State Pedagogical University. It was attended by 30 respondents. After the meeting, employees were asked to undergo training on the organization of personal effectiveness. The training was conducted successfully and, based on the data obtained, we concluded that there is a need for further closer cooperation between organizations, therefore, we presented a plan of interaction between Nizhny Novgorod State Pedagogical University named after Kozma Minin and the Registration Agency limited liability company, the result of which in the future should be the achievement of benefits for both parties, that is, an increase in the intensification of the organization’s activities "and The effectiveness of the training of future graduates of the “Service” field of study.El artículo revela la estructura del contenido de la capacitación interna y el reciclaje de los empleados, refleja los principios en los que se basa la capacitación y las funciones que realiza la capacitación profesional interna. En todo el mundo y en Rusia en particular, el tema de mayor atención es la introducción de la tecnología de la información. Por lo tanto, los autores consideran la capacitación interna de los empleados de la empresa de servicios utilizando las tecnologías designadas. La agencia, la Agencia de Registro, una compañía de responsabilidad limitada, fue considerada en el artículo. En 2018, entre sus empleados, se realizó una encuesta que reveló el deseo de los empleados de mejorar sus habilidades a través de cursos electrónicos proporcionados por la Universidad Pedagógica Estatal de Nizhny Novgorod. Asistieron 30 encuestados. Después de la reunión, se pidió a los empleados que recibieran capacitación sobre la organización de la efectividad personal. La capacitación se realizó con éxito y, en base a los datos obtenidos, concluimos que existe la necesidad de una cooperación más estrecha entre las organizaciones, por lo tanto, presentamos un plan de interacción entre la Universidad Pedagógica del Estado de Nizhny Novgorod, cuyo nombre es Kozma Minin y la Agencia de Registro limitada empresa de responsabilidad, cuyo resultado en el futuro debería ser el logro de beneficios para ambas partes, es decir, un aumento en la intensificación de las actividades de la organización "y la efectividad de la capacitación de futuros graduados del campo de estudio" Servicio"

Pharmacological correction of L-NAME-induced oxide deficiency with derivatives of 3-(2,2,2-trimethylhydrazinium) propionate

This paper deals with the study of correction of L-NAME-induced endothelial dysfunction by means of 3-(2,2,2-trimethylhydrazinium) propionate derivative

Calcium Signaling Initiated by Agonists in Mesenchymal Stromal Cells from the Human Adipose Tissue

Mesenchymal stromal cells (MSCs) from different sources represent a heterogeneous population of proliferating non-differentiated cells that contain multipotent stem cells capable of originating a variety of mesenchymal cell lineages. By using Ca2+ imaging and the Ca2+ dye Fluo-4, we studied MSCs from the human adipose tissue and examined Ca2+ signaling initiated by a variety of GPCR ligands, focusing primarily on adrenergic and purinergic agonists. Being characterized by a relative change of Fluo-4 fluorescence, agonist-induced Ca2+ responses were generated in an “all-or-nothing” fashion. Specifically, at relatively low doses, agonists elicited undetectable responses but initiated quite similar Ca2+ transients at all concentrations above the threshold. The inhibitory analysis and Ca2+/IP3 uncaging pointed at the phosphoinositide cascade as a pivotal pathway responsible for agonist transduction and implicated Ca2+-induced Ca2+ release (CICR) in shaping agonists-dependent Ca2+ signals. Altogether, our data suggest that agonist transduction in MSCs includes two fundamentally different stages: an agonist initially triggers a local, gradual, and relatively small Ca2+ signal, which next stimulates CICR to accomplish transduction with a large and global Ca2+ transient. By involving the trigger-like mechanism CICR, a cell is capable of generating Ca2+ responses of virtually universal shape and magnitude at different agonist concentrations above the threshold

CORRELATION ANALYSIS BETWEEN LABORATORY IMMUNE AND METABOLIC PARAMETERS IN CHRONIC BRAIN ISCHEMIA STAGES I AND II ALONG WITH HYPERTENSION AFTER TREATMENT

Determination of interrelationships between impairments of laboratory parameters of immune and metabolic status in patients with chronic cerebral ischemia of I-II stages was carried out. The study included 104 patients, of which 76 were female and 28 males, with CCI on the background of II degree hypertension, of which 52 patients were with stage I and 52 with stage II at the age of 50 ± 5 years. Also, clinical and laboratory parameters were studied in 22 healthy donors of the same age. Evaluation of clinical and laboratory data was carried out at the beginning of treatment and 2 weeks after its end. The sorption capacity of erythrocytes and the sorption capacity of the glycocalyx (SEG), the activity of lipid peroxidation processes, the state of the antioxidant system were determined in blood plasma and erythrocytes, the level of stable metabolites of nitric oxide (SMNO), neopterin, C-reactive protein, cytokines (TNFα, IL- 1β, IL-8, IFNγ, IL-18, G-CSF, IL-4, IL-10), immunoglobulins (IgM, IgG, IgA), complement system components (C3, C4, C5, C5А), the phagocytic and oxygen-dependent activity of polymorphonuclear blood leukocytes. Comparative assessment of the results of correlation, factorial and cluster analyzes for assessing the parameters of the immune and metabolic status in patients with stage I CCI revealed the most significant laboratory parameters necessary for determination in the clinic for objective assessment of the severity of immune and metabolic disorders: TNFα, IL-8, IL-10, SMNO and NEG. In patients with CCI stage II, to objectively assess the severity of immune and metabolic disorders, TNFα, IL-8, IL-17, IL-10, the phagocytic number of neutrophils and SEG are recommended

PERSONALIZED PHARMACOLOGICAL CORRECTION OF IMMUNE SYSTEMS, METABOLIC AND NEUROPSYCHIC PARAMETERS IN CHRONIC BRAIN ISCHEMIA OF STAGE I AND II ON THE BACKGROUND OF HYPERTENSION DISEASE

The study aimed to develop a personalized pharmacological correction of immune, metabolic and neuropsychiatric disorders in chronic cerebral ischemia (CCI) stages I and II. The study included 104 patients, of which 76 were female and 28 were male, with CCI on the background of grade II hypertension, of which 52 patients were with stage I and 52 with stage II at the age of 50±5 years. Clinical and laboratory parameters were studied in 22 healthy donors of the same age who formed a control group. Patients with CCI were randomized according to gender, age, treatment method, concomitant pathology, and duration of the disease. Evaluation of clinical and laboratory data was carried out at the beginning of treatment and 2 weeks after its end. The sorption capacity of erythrocytes and the sorption capacity of the glycocalyx (SEG), the activity of lipid peroxidation processes, the state of the antioxidant system were determined in blood plasma and erythrocytes, the level of stable metabolites of nitric oxide (SMNO), neopterin, C-reactive protein, cytokines (TNFα, IL-1β, IL-8, IFNγ, IL-18, G-CSF, IL-4, IL-10), immunoglobulins (IgM, IgG, IgA), complement system components (C3, C4, C5, C5A), phagocytic and oxygen-dependent activity of polymorphonuclear blood leukocytes. It has been established that for patients with CCI I with high concentrations of IL-8, IL-10, SMNO and a low SEG index, the intake of Cereton and Actovegin or Ceraxon and Mexicor will be insufficient for effective correction of immunometabolic disorders, which requires additional administration of an immunomodulator. Patients with CCI II, who have a higher plasma level of TNFα, IL-10 and low SEG values, need to prescribe Ceraxon, Mexicor and Glutoxim or Ceraxon, Mexicor and Polyoxidonium in order to obtain the maximum clinical and laboratory positive effect